Col11a1 (NM_007729) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	C530001D20Rik; cho
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR212085 representing NM_007729 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCCCTGGTCCAGGTGGAAAACGAAACGGTGGATCTGGGATCTCACCATCTCAACCCTCGCCTTGA CTTTTCTCTTCCAAGCTAGAGAGGTCAGAGGAGCTGCTCCAGTTGATATACTAAAAGCATTAGATTTTCA CAATTCTCCAGTGGGAATATCTAAAACAACAGGATTTTGCACAAACAGAAAGAATTCTAAAGATCCAGAT GTTGCTTATAGAGTTACGGAGGAAGCACAAATCAGCGCCCCAACAAAGCAGCTATTTCCAGGAGGAATTT TTCCACAAGATTTTTCAATACTATTTACAATAAAACCCAAAAAAGGAACTCAAGCTTTCCTCTTGTCCCT CTACAATGAACATGGCATTCAGCAACTCGGTGTTGAGGTTGGGAGATCGCCCGTTTTTCTATTTGAAGAC CACACTGGAAAACCCACACCGGAAAACTATCCTCTCTTCTCAACAGTTAACATTGCTGATGGCAAGTGGC ACCGGGTCGCAATCAGTGTGGAGAAGAAAACTGTGACAATGATTGTTGATTGTAAGAAGAAAATCACAAA ACCCCTCGATAGAAGTGAGAGATCGATAGTCGATACCAATGGAATCATGGTATTTGGAACAAGAATTTTA GAAACAGATGTTTTCCAGGGTGATATTCAGCAGTTCCTGATCACAGGAGACCCCAAGGCAGCATACGACT ACTGTGACCATTACAGTCCAGATTGTGACTTAACATCCAAGGCTGCCCAGGCTCAGGAGCCTCACATTGA TGAGTATGCACCTGAGGATATAATCGAGTATGACTATGAATATGGGGAGACAGACTATAAAGAGGCTGAG AGTGTAACAGAGATGCCTACTTTTACTGAAGAAACAGTAGCACAAACAGAGGCAAATATTGTGGATGATT TTCAAGACTACAACTATGGAACAATGGAACCTTACCAGACTGAGACTCCTAGGCGGGTATCAGGATCGAA TGAGCCAAATCCAGTTGAAGAAGGTTTTACTGAAGAATATCTAACCGGAGAGGATTATGATGTCCAGAGA AACACTTCCGAGGATATTCTGTACGGGAACAAAGGAGTAGACGGCAGGGATTCTGATCTCCTGGTAGATG GAGATTTAGGTGAATATGATTTTTATGAATACAAAGAATATGAAGAAAGGACTACGACCTCCCCTAATGA AGAATTTGGCCCAGGTGTCCCAGCAGAAACTGATTTCACAGAAACAAGCATAAATGGACATGGTGCATAT GGAGAGAAAGGGCAGAAGGGGGAACCGGCTGTGGTTGAACCCGGGATGCTTGTTGAAGGACCACCTGGGC CAGCAGGACCAGCGGGTCTTATGGGTCCTCCAGGGCTACAAGGTCCTTCTGGGCTTCCCGGCGACCCTGG GGATAGGGGCCCACCAGGACGTCCTGGCTTACCAGGGGCTGATGGTTTACCTGGACCTCCTGGAACCATG CTGATGTTACCATTCCGCTATGGGGGTGACGGCTCCAAAGGACCAACAATCTCTGCACAGGAAGCCCAAG CTCAGGCGATTCTTCAGCAGGCTCGGATTGCTCTGAGAGGCCCTCCTGGCCCAATGGGTCTTACTGGAAG ACCAGGTCCTGTGGGGGGTCCTGGTTCAGCTGGAGCCAAAGGTGAGAGTGGGGATCCAGGTCCTCAGGGT CCTCGAGGTGTCCAAGGTCCTCCTGGTCCAACGGGAAAGCCTGGAAAGAGGGGCCGTCCAGGTGCTGATG GAGGAAGAGGCATGCCAGGAGAATCTGGGTCTAAGGGAGACCGAGGGTTTGATGGACTTCCAGGTCTGCC TGGTGACAAAGGTCACAGGGGAGAAAGAGGTCCACAAGGTCCACCTGGCCTCCCTGGCGATGATGGAATG AGGGGAGAAGATGGGGAGATCGGGCCCAGGGGTCTTCCAGGTGAAGCTGGTCCAAGAGGCTTGCTGGGAC CAAGGGGAACTCCAGGACCCCCTGGGCAGCCTGGTATCGGAGGTATAGATGGCCCCCAAGGACCAAAAGG AAACATGGGTCCCCAAGGGGAGCCTGGACCACCAGGTCAGCAAGGAAATCCAGGACCTCAAGGACTTCCT GGTCCACAAGGCCCAATTGGCCCTCCAGGAGAAAAAGGGCCACAAGGAAAACCAGGGCTTGCGGGACTTC CTGGTGCTGATGGACCTCCTGGTCATCCTGGGAAAGAAGGCCAGTCTGGAGAAAAGGGTGCCCTGGGTCC TCCTGGTCCTCAGGGGCCTATTGGTTATCCTGGTCCCCGTGGAGTAAAGGGAGCAGATGGTGTCAGAGGT CTCAAGGGCTCTAAAGGCGAAAAGGGTGAAGATGGCTTTCCAGGATTCAAAGGTGACATGGGTCTTAAAG GTGACAGAGGCGAGGTGGGTCAAGTTGGCCCAAGAGGAGAAGATGGCCCTGAAGGCCCCAAAGGCCGTGC AGGCCCAACTGGAGACCCAGGTCCCTCTGGCCAAGCAGGAGAGAAGGGAAAACTTGGGGTTCCAGGATTG CCGGGATATCCAGGAAGACAAGGTCCAAAGGGATCCACTGGATTTCCTGGATTTCCAGGTGCCAACGGGG AGAAAGGTGCCCGGGGAATTGCTGGCAAACCAGGCCCCCGGGGACAGCGTGGTCCAACGGGTCCTCGAGG TTCCAGAGGCGCCAGAGGTCCCACGGGAAAACCTGGTCCGAAGGGTACTTCAGGTGGTGATGGCCCTCCT GGTCCTCCAGGCGAAAGGGGCCCTCAAGGACCTCAAGGTCCAGTTGGATTCCCTGGACCAAAAGGCCCCC CTGGCCCTGCTGGGAAAGATGGGCTACCAGGACACCCTGGGCAGCGTGGTGAGACTGGATTTCAAGGCAA GACAGGTCCCCCTGGACCAGGAGGTGTGGTAGGACCACAGGGACCAACTGGTGAGACTGGTCCTATAGGT GAACGTGGCCATCCCGGCCCTCCTGGTCCTCCTGGCGAGCAAGGACTTCCCGGTGCTGCAGGGAAAGAAG GTGCAAAGGGTGATCCCGGGCCTCAAGGTATCTCAGGAAAAGATGGACCAGCAGGAATACGCGGTTTCCC AGGTGAAAGAGGCCTTCCTGGAGCCCAGGGTGCACCTGGGCTGAAAGGAGGAGAAGGTCCCCAGGGCCCA CAGGGTCCAGTTGGCTCTCCTGGAGAGCGAGGCTCAGCAGGCACAGCAGGCCCAATTGGTTTGCCAGGAC GTCCAGGCCCCCAGGGTCCTCCTGGTCCAGCTGGAGAAAAAGGTGCTCCTGGAGAAAAGGGTCCCCAGGG TCCTGCAGGTAGAGATGGTGTCCAAGGCCCTGTGGGTCTCCCAGGACCTGCTGGTCCTGCTGGCTCTCCT GGAGAAGATGGAGACAAGGGTGAAATTGGTGAACCAGGCCAAAAGGGCAGCAAAGGTGACAAAGGCGAAA ATGGTCCTCCTGGTCCCCCCGGACTTCAAGGACCTGTTGGTGCTCCTGGAATCGCTGGAGGCGATGGTGA GCCAGGTCCCCGAGGACAGCAGGGAATGTTTGGACAAAAAGGTGATGAGGGCGCACGAGGTTTCCCAGGA CTTCCTGGCCCCATAGGTCTTCAGGGGTTGCCAGGCCCACCAGGTGAAAAGGGCGAAAATGGAGACGTTG GCCCCATGGGTCCACCAGGTCCTCCTGGCCCACGAGGCCCTCAAGGTCCCAATGGAGCTGATGGACCACA AGGACCCCCAGGCTCCATTGGTTCAGTTGGTGTTGTGGGAGACAAGGGTGAACCTGGAGAAGCAGGGAAC CCAGGGCCCCCTGGGGAAGCTGGCTCTGGTGGTCTCAAAGGAGAGAGAGGAGAGAAAGGAGAAGCTGGGC CCCCTGGTGCTGCAGGTCCTGCTGGTATTAAGGGGCCACCAGGTGATGATGGGCCCAAGGGGAACCCGGG ACCCGTTGGGTTTCCTGGAGATCCTGGTCCACCTGGAGAACCTGGTCCTGCAGGGCAAGATGGTGTTGGA GGTGACAAGGGTGAAGATGGAGATCCAGGACAACCAGGTCCCCCTGGTCCATCTGGTGAAGCTGGCCCTC CAGGTCCTCCTGGGAAGAGAGGTCCGCCTGGAGCTTCAGGTTCAGAAGGAAGGCAAGGAGAAAAAGGTGC TAAGGGAGAAGCTGGTGCTGAAGGACCTCCTGGGAAAACTGGCCCTGTCGGACCTCAGGGACCTTCTGGA AAGCCTGGTCCAGAAGGTCTTCGAGGCATCCCTGGTCCTGTGGGAGAACAAGGTCTCCCAGGTGCTGCTG GCCAAGATGGACCTCCTGGTCCTCTGGGACCTCCTGGTTTACCTGGTCTCAAAGGTGACCCTGGATCCAA GGGTGAAAAGGGACATCCTGGTTTAATTGGATTGATTGGTCCTCCAGGAGAACAAGGGGAAAAAGGTGAC AGAGGACTTCCTGGGACTCAAGGTTCTCCAGGAGCCAAAGGCGATGGGGGCATCCCAGGTCCTGCTGGTC CCATAGGTCCCCCTGGTCCTCCAGGATTACCAGGTCCCGCTGGCCCGAAGGGTAACAAAGGATCATCTGG ACCTACTGGCCAGAAGGGTGACAGTGGTATGCCAGGGCCTCCCGGGCCTCCAGGTCCTCCTGGAGAAGTG ATACAGCCTTTGCCTATTTTGTCACCAAAAAAGACCAGAAGACACACTGAAAGCATCCAGGGTGATGCAG GAGATAATATTCTTGACTACTCAGATGGCATGGAGGAGATATTTGGTTCCCTCAATTCTCTGAAACAAGA CATTGAACACATGAAGTTTCCCATGGGCACACAGACCAACCCAGCACGAACATGCAAAGACCTGCAACTC AGCCATCCCGACTTCCCAGATGGTGAATATTGGATTGATCCTAACCAAGGTTGTTCAGGTGATTCCTTCA AAGTTTACTGTAATTTCACAGCTGGTGGTGAGACATGCATCTATCCGGATAAAAAATCTGAGGGAGTAAG AATTTCATCGTGGCCAAAGGAGAAACCAGGAAGTTGGTACAGTGAATTTAAGAGAGGAAAACTGCTTTCA TATTTAGATGTGGAAGGCAATTCCATAAATATGGTACAAATGACATTCCTGAAGCTCCTGACTGCCTCTG CCCGGCAAAACTTCACCTACAATTGCCATCAGTCAGCTGCCTGGTATGACGTATTATCAGGAAGTTATGA CAAAGCACTTCGATTTCTGGGATCAAATGATGAGGAAATGTCCTATGAGAACAACCCACACATCAAAGCT TTGTATGATGGCTGTGCGTCTCGAAAAGGCTATGAAAAGACAGTGATTGAGATCAATACTCCGAAAATTG ATCAGGTACCCATCATTGATGTAATGATCAATGATTTTGGTGATCAGAACCAGAAGTTTGGATTTGAAGT CGGTCCAGCTTGCTTTCTTGGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR212085 representing NM_007729 Red=Cloning site Green=Tags(s) MEPWSRWKTKRWIWDLTISTLALTFLFQAREVRGAAPVDILKALDFHNSPVGISKTTGFCTNRKNSKDPD VAYRVTEEAQISAPTKQLFPGGIFPQDFSILFTIKPKKGTQAFLLSLYNEHGIQQLGVEVGRSPVFLFED HTGKPTPENYPLFSTVNIADGKWHRVAISVEKKTVTMIVDCKKKITKPLDRSERSIVDTNGIMVFGTRIL ETDVFQGDIQQFLITGDPKAAYDYCDHYSPDCDLTSKAAQAQEPHIDEYAPEDIIEYDYEYGETDYKEAE SVTEMPTFTEETVAQTEANIVDDFQDYNYGTMEPYQTETPRRVSGSNEPNPVEEGFTEEYLTGEDYDVQR NTSEDILYGNKGVDGRDSDLLVDGDLGEYDFYEYKEYEERTTTSPNEEFGPGVPAETDFTETSINGHGAY GEKGQKGEPAVVEPGMLVEGPPGPAGPAGLMGPPGLQGPSGLPGDPGDRGPPGRPGLPGADGLPGPPGTM LMLPFRYGGDGSKGPTISAQEAQAQAILQQARIALRGPPGPMGLTGRPGPVGGPGSAGAKGESGDPGPQG PRGVQGPPGPTGKPGKRGRPGADGGRGMPGESGSKGDRGFDGLPGLPGDKGHRGERGPQGPPGLPGDDGM RGEDGEIGPRGLPGEAGPRGLLGPRGTPGPPGQPGIGGIDGPQGPKGNMGPQGEPGPPGQQGNPGPQGLP GPQGPIGPPGEKGPQGKPGLAGLPGADGPPGHPGKEGQSGEKGALGPPGPQGPIGYPGPRGVKGADGVRG LKGSKGEKGEDGFPGFKGDMGLKGDRGEVGQVGPRGEDGPEGPKGRAGPTGDPGPSGQAGEKGKLGVPGL PGYPGRQGPKGSTGFPGFPGANGEKGARGIAGKPGPRGQRGPTGPRGSRGARGPTGKPGPKGTSGGDGPP GPPGERGPQGPQGPVGFPGPKGPPGPAGKDGLPGHPGQRGETGFQGKTGPPGPGGVVGPQGPTGETGPIG ERGHPGPPGPPGEQGLPGAAGKEGAKGDPGPQGISGKDGPAGIRGFPGERGLPGAQGAPGLKGGEGPQGP QGPVGSPGERGSAGTAGPIGLPGRPGPQGPPGPAGEKGAPGEKGPQGPAGRDGVQGPVGLPGPAGPAGSP GEDGDKGEIGEPGQKGSKGDKGENGPPGPPGLQGPVGAPGIAGGDGEPGPRGQQGMFGQKGDEGARGFPG LPGPIGLQGLPGPPGEKGENGDVGPMGPPGPPGPRGPQGPNGADGPQGPPGSIGSVGVVGDKGEPGEAGN PGPPGEAGSGGLKGERGEKGEAGPPGAAGPAGIKGPPGDDGPKGNPGPVGFPGDPGPPGEPGPAGQDGVG GDKGEDGDPGQPGPPGPSGEAGPPGPPGKRGPPGASGSEGRQGEKGAKGEAGAEGPPGKTGPVGPQGPSG KPGPEGLRGIPGPVGEQGLPGAAGQDGPPGPLGPPGLPGLKGDPGSKGEKGHPGLIGLIGPPGEQGEKGD RGLPGTQGSPGAKGDGGIPGPAGPIGPPGPPGLPGPAGPKGNKGSSGPTGQKGDSGMPGPPGPPGPPGEV IQPLPILSPKKTRRHTESIQGDAGDNILDYSDGMEEIFGSLNSLKQDIEHMKFPMGTQTNPARTCKDLQL SHPDFPDGEYWIDPNQGCSGDSFKVYCNFTAGGETCIYPDKKSEGVRISSWPKEKPGSWYSEFKRGKLLS YLDVEGNSINMVQMTFLKLLTASARQNFTYNCHQSAAWYDVLSGSYDKALRFLGSNDEEMSYENNPHIKA LYDGCASRKGYEKTVIEINTPKIDQVPIIDVMINDFGDQNQKFGFEVGPACFLG TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_007729
ORF Size	5412 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_007729.3, NP_031755.2
RefSeq Size	7705 bp
RefSeq ORF	5415 bp
Locus ID	12814
UniProt ID	Q61245
MW	181 kDa
Gene Summary	This gene encodes the alpha-1 subunit of type XI collagen, one of the low abundance fibrillar collagens that is essential for normal embryonic skeletal development and the cohesive properties of cartilage. The encoded protein, in association with the alpha-1 subunit of type II collagen, forms a heterotrimeric type XI procollagen that undergoes proteolytic processing. Mice lacking the encoded protein develop severe chondrodysplasia and die at birth. [provided by RefSeq, Dec 2015]

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