Hcfc1 (NM_008224) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	AW060991; HCF; HCF1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR212109 representing NM_008224 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTTCGGCTGTGTCTCCCGCAAACTTGCCAGCGGTGCTTCTGCAGCCCCGCTGGAAACGGGTGGTGG GCTGGTCGGGTCCCGTGCCCCGACCCCGCCACGGCCACCGTGCAGTGGCTATCAAGGAGCTTATAGTGGT GTTTGGCGGCGGCAACGAGGGGATAGTGGACGAACTACACGTGTACAACACTGCAACCAACCAGTGGTTC ATCCCAGCTGTGAGAGGGGATATCCCTCCAGGGTGTGCAGCCTATGGCTTTGTGTGTGATGGTACTCGCC TATTGGTGTTTGGTGGAATGGTAGAGTATGGAAAATACAGCAACGACCTCTATGAACTCCAGGCAAGTCG TTGGGAATGGAAGAGACTGAAGGCAAAGACACCCAAAAATGGGCCTCCTCCATGTCCTCGGCTTGGACAT AGCTTCTCCCTTGTGGGCAACAAATGTTACCTGTTTGGGGGTCTGGCCAATGATAGTGAGGACCCCAAGA ACAACATTCCGAGGTACCTGAATGACTTATATATTCTCGAACTACGGCCAGGCTCTGGAGTGGTAGCTTG GGACATCCCCATCACTTACGGTGTCCTGCCTCCACCCCGGGAGTCACATACTGCTGTGGTCTACACTGAA AAAGATAACAAGAAATCCAAGCTGGTGATCTATGGAGGGATGAGTGGCTGCAGGCTAGGGGACCTTTGGA CCCTGGACATTGAGACACTGACATGGAATAAGCCCAGCCTTAGTGGGGTGGCACCCCTTCCTCGCAGCCT CCACTCTGCAACCACCATAGGAAACAAAATGTATGTATTTGGTGGCTGGGTGCCCCTTGTCATGGACGAT GTCAAAGTGGCCACACACGAGAAGGAGTGGAAGTGTACCAACACACTGGCTTGTCTCAACCTGGATACCA TGGCCTGGGAAACCATCCTGATGGATACATTGGAGGACAACATTCCTCGAGCTCGAGCAGGCCACTGTGC TGTTGCCATCAATACTCGTCTGTATATTTGGAGTGGCCGTGATGGCTACCGCAAGGCCTGGAACAACCAG GTCTGCTGCAAGGACTTGTGGTATTTGGAGACAGAAAAGCCACCACCCCCAGCCCGAGTACAACTAGTAC GAGCCAACACCAACTCACTGGAGGTTAGCTGGGGTGCAGTGGCAACAGCTGACAGTTACCTTCTACAACT CCAGAAATATGACATTCCTGCCACAGCTGCTACGGCTACCTCCCCCACTCCCAATCCAGTCCCGTCTGTG CCTGCCAACCCTCCCAAGAGCCCTGCGCCAGCAGCAGCTGCACCTGCTGTACAGCCACTGACCCAAGTAG GCATCACACTTGTGCCCCAGGCTGCCACTGCACCCCCAAGCACAACCACCATCCAGGTCTTGCCGACAGT GCCAGGCAGCTCCATTTCTGTGCCCACTGCAGCCAGGACTCAAGGTGTCCCTGCTGTTCTCAAAGTGACT GGTCCTCAAGCTACAACAGGAACACCACTGGTTACCATGAGACCTGCAAGCCAGGCTGGAAAAGCTCCTG TCACTGTGACTTCCCTGCCTGCCAGTGTTCGAATGGTTGTACCCACACAGAGTGCCCAGGGGACGGTGAT CGGCAGCAACCCACAGATGAGTGGGATGGCCGCATTGGCTGCTGCTGCTGCTGCCACACAGAAAATCCCT CCATCCTCAGCACCCACGGTGCTGAGTGTCCCAGCAGGGACCACCATCGTCAAGACAGTGGCTGTGACAC CTGGCACGACCACTCTTCCAGCCACTGTGAAGGTGGCCTCCTCCCCTGTCATGGTGAGCAACCCAGCCAC TCGAATGCTAAAGACTGCAGCTGCCCAAGTGGGGACATCTGTGTCCTCTGCTGCCAACACATCTACTCGC CCTATCATCACAGTACACAAATCAGGAACTGTAACAGTGGCCCAGCAAGCCCAGGTGGTGACCACGGTGG TAGGTGGAGTCACCAAGACCATCACCCTAGTGAAGAGCCCCATCTCTGTCCCAGGAGGCAGTGCTCTGAT TTCCAATCTGGGAAAAGTGATGTCGGTGGTCCAGACCAAACCAGTTCAGACATCAGCAGTGACAGGCCAA GCATCTACAGGTCCTGTGACTCAGATCATCCAGACCAAAGGACCCCTGCCAGCGGGGACTATCCTGAAGC TGGTGACATCAGCAGATGGCAAGCCCACAACCATCATTACCACCACACAGGCTAGTGGGGCAGGGACCAA GCCCACTATCCTGGGCATCAGTAGTGTTTCTCCCAGCACCACCAAACCTGGCACAACTACCATTATTAAG ACCATTCCTATGTCGGCCATTATCACCCAGGCAGGTGCCACAGGTGTTACCAGCAGTCCTGGCATTAAGT CCCCAATTACAATTATCACCACCAAAGTGATGACTTCAGGAACAGGAGCGCCTGCTAAAATCATCACTGC TGTCCCCAAGATTGCTACTGGCCATGGGCAACAAGGAGTGACCCAGGTGGTGCTAAAGGGGGCCCCTGGA CAACCAGGCACCATCCTCCGTACTGTGCCTATGGGCGGCGTTCGCCTGGTCACCCCTGTCACCGTCTCTG CTGTCAAGCCAGCTGTCACCACATTGGTTGTGAAGGGTACCACAGGTGTTACAACGCTAGGCACAGTGAC AGGCACTGTCTCCACCAGCCTGGCCGGAGCTGGGGCACATAGCACCAGTGCTTCCCTGGCTACACCTATC ACTACCTTGGGCACTATTGCTACGCTCTCAAGCCAGGTGATCAACCCTACTGCTATCACAGTGTCAGCTG CACAGACTACACTAACAGCTGCTGGTGGGCTTACCACACCCACAATCACAATGCAGCCTGTCTCCCAGCC TACCCAGGTCACTCTGATTACAGCACCCAGTGGGGTTGAAGCACAGCCTGTACATGACCTTCCTGTATCC ATTTTGGCCTCACCTACTACAGAGCAGCCCACAGCAACAGTCACCATCGCTGACTCAGGCCAGGGTGATG TGCAGCCCGGCACTGTGACACTGGTGTGTTCCAACCCACCCTGTGAAACCCATGAAACAGGCACCACCAA CACAGCTACCACCACTGTTGTGGCTAACCTTGGTGGACATCCTCAACCTACCCAGGTGCAGTTTGTTTGT GACAGACAGGAGACAGCTGCTTCACTTGTGACCTCAGCTGTAGGACAACAGAATGGTAATGTGGTCCGTG TCTGTTCAAACCCCCCCTGTGAGACCCATGAGACGGGCACTACCAACACTGCCACAACAGCCACCTCCAA CATGGCTGGGCAGCATGGCTGCTCGAACCCCCCCTGTGAGACTCATGAGACAGGCACCACCAGCACTGCC ACTACAGCAATGTCCAGCATGGGCACTGGGCAGCAGCGAGACACTCGTCGTACCACTAACACCCCCACTG TAGTGCGGATCACTGTGGCTCCTGGGGCATTGGAGAGAGTCCAGGGTACCGTGAAGCCTCAGTGCCAAAC CCAGCAGACCAACATGACCACCACCACCATGACTGTGCAGGCCACTGGAGCTCCATGCTCAGCTGGCCCC CTGCTTAGGCCAAGTGTGGCACTGGAGTCTGGGAGCCACAGCCCTGCCTTTGTGCAACTAGCCCTTCCAA GTGTCAGAGTTGGGCTAAGTGGCCCCAGCAGCAAGGACATGCCCACAGGGCGCCAACCAGAGACATATCA TACTTACACAACTAATACCCCAACCACAACCCGCTCTATCATGGTTGCTGGGGAGCTTGGTGCAGCTCGG GTGGTCCCCACATCTACATATGAGAGCCTCCAGGCAAGCTCTCCTAGCAGCACCATGACTATGACAGCCC TAGAGGCACTGCTGTGCCCTTCGGCTACTGTCACCCAAGTCTGCTCCAACCCGCCATGTGAGACCCATGA GACGGGTACCACCAACACCGCCACTACCTCCAATGCGGGCAGTGCTCAGCGAGTATGCTCCAACCCGCCT TGTGAGACTCATGAGACGGGCACCACACACACAGCTACCACTGCCACATCAAATGGAGGTGCAGGCCAGC CTGAGGGTGGACAACAGCCTGCCAGTGGCCATCCCTGCGAGACACACCAGACCACTTCCACTGGCACCAC TATGTCAGTCAGTGTGGGTACCCTGATTCCTGATGCTACTTCCTCTCATGGAACCCTGGAGTCGGGCTTA GAGGTGGTAGCAGTGCCCACTGTCACCTCCCAGGCTGGTTCCACATTGCTGGCCTCTTTCCCAACACAGA GGGTATGCTCCAACCCTCCTTGCGAGACCCACGAGACAGGTACCACGCACACAGCCACCACTGTCACCTC TAACATGAGCTCAAACCAAGACCCTCCACCAGCTGCCAGTGACCAAGGAGAGGTGGCAAGCACCCAAGGT GACAGCACAAATATCACCAGTGCCAGTGCTATCACTACAAGTGTGTCTTCTACATTGCCACGAGCAGTGA CCACTGTGACACAGTCTACACCAGTCCCTGGTCCCTCTGTGCCGCCCCCAGAGGAACTCCAGGTCTCACC AGGGCCTCGCCAGCAGCTGCCTCCACGGCAACTCCTGCAGTCTGCCTCCACACCCCTGATGGGGGAGTCT ACTGAGGTCCTGTCAGCCTCCCAGACCCCTGAGCTCCAGGCCGCCGTGGATCTGAGCAGCACTGGGGACC CATCTTCAGGCCAGGAGCCTACCACCTCTGCTGTCGTGGCCACTGTGGTGGTCCAACCACCCCCACCCAC ACAGTCTGAAGTAGACCAGTTATCACTTCCCCAAGAGCTGATGGCTGAAGCCCAGGCGGGCACCACAACC CTTATGGTAACAGGGCTCACTCCAGAGGAGCTGGCAGTGACTGCTGCTGCTGAAGCAGCTGCTCAAGCTG CAGCCACTGAAGAAGCCCAAGCCTTGGCCATCCAGGCTGTGCTCCAGGCCGCACAGCAGGCTGTCATGGG CACTGGGGAGCCCATGGATACATCTGAAGCAGCAGCAGCAGTGACACAAGCAGAACTGGGTCACCTTTCA GCTGAAGGCCAAGAGGGTCAGGCTACCACCATACCCATTGTGTTGACACAGCAGGAGCTTGCAGCCCTGG TGCAGCAGCAGCAGCAGCTCCAGGAGGCTCAAGCTCAAGCCCAGCAACAGCACCATCTTCCCACTGAGGC TCTGGCCCCAGCTGACAGTCTCAATGACCCATCCATCGAGAGCAACTGCCTCAACGAGTTAGCTAGTGCT GTCCCAAGCACCGTGGCTTTGCTACCCTCAACAGCTACCGAGAGCCTGGCTCCATCTAACACATTTGTGG CTCCCCAGCCTGTTGTAGCTAGTCCAGCAAAGATGCAGGCTGCAGCTACCCTTACTGAAGTGGCCAATGG CATTGAGTCCCTGGGTGTGAAACCGGACTTGCCACCCCCACCCAGCAAAGCCCCTGTGAAAAAGGAGAAC CAGTGGTTTGATGTGGGGGTCATTAAGGGTACCAGTGTAATGGTGACACACTATTTTCTGCCACCAGATG ATGCTGTTCAGTCAGATGATGACTCAGGCACGGTCCCAGACTATAACCAGCTAAAGAAGCAGGAGCTACA GCCAGGCACGGCTTACAAATTTCGAGTTGCTGGAATCAATGCTTGTGGCCGGGGACCCTTCAGTGAGATC TCAGCCTTTAAGACTTGTCTGCCTGGGTTCCCAGGGGCTCCTTGTGCTATTAAAATCAGCAAGAGCCCAG ATGGTGCTCACCTCACCTGGGAGCCACCGTCTGTGACCTCCGGCAAGATCATCGAGTACTCTGTGTACCT GGCCATCCAGAGCTCACAGGCCAGTGGTGAGCCAAAGAGCTCCACCCCAGCCCAGCTGGCCTTCATGCGA GTGTACTGTGGGCCTAGCCCTTCCTGCCTAGTGCAGTCCTCCAGCCTCTCCAACGCCCACATTGACTATA CTACAAAGCCTGCCATCATCTTCCGCATTGCTGCCCGCAATGAAAAGGGCTACGGCCCTGCCACACAAGT GAGGTGGTTGCAAGAAACTAGTAAAGACAGCTCTGGCACCAAGCCGGCCAGCAAGCGGCCCATGTCGTCT CCAGAAATGAAATCTGCTCCAAAGAAGTCTAAGGCTGATGGTCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR212109 representing NM_008224 Red=Cloning site Green=Tags(s) MASAVSPANLPAVLLQPRWKRVVGWSGPVPRPRHGHRAVAIKELIVVFGGGNEGIVDELHVYNTATNQWF IPAVRGDIPPGCAAYGFVCDGTRLLVFGGMVEYGKYSNDLYELQASRWEWKRLKAKTPKNGPPPCPRLGH SFSLVGNKCYLFGGLANDSEDPKNNIPRYLNDLYILELRPGSGVVAWDIPITYGVLPPPRESHTAVVYTE KDNKKSKLVIYGGMSGCRLGDLWTLDIETLTWNKPSLSGVAPLPRSLHSATTIGNKMYVFGGWVPLVMDD VKVATHEKEWKCTNTLACLNLDTMAWETILMDTLEDNIPRARAGHCAVAINTRLYIWSGRDGYRKAWNNQ VCCKDLWYLETEKPPPPARVQLVRANTNSLEVSWGAVATADSYLLQLQKYDIPATAATATSPTPNPVPSV PANPPKSPAPAAAAPAVQPLTQVGITLVPQAATAPPSTTTIQVLPTVPGSSISVPTAARTQGVPAVLKVT GPQATTGTPLVTMRPASQAGKAPVTVTSLPASVRMVVPTQSAQGTVIGSNPQMSGMAALAAAAAATQKIP PSSAPTVLSVPAGTTIVKTVAVTPGTTTLPATVKVASSPVMVSNPATRMLKTAAAQVGTSVSSAANTSTR PIITVHKSGTVTVAQQAQVVTTVVGGVTKTITLVKSPISVPGGSALISNLGKVMSVVQTKPVQTSAVTGQ ASTGPVTQIIQTKGPLPAGTILKLVTSADGKPTTIITTTQASGAGTKPTILGISSVSPSTTKPGTTTIIK TIPMSAIITQAGATGVTSSPGIKSPITIITTKVMTSGTGAPAKIITAVPKIATGHGQQGVTQVVLKGAPG QPGTILRTVPMGGVRLVTPVTVSAVKPAVTTLVVKGTTGVTTLGTVTGTVSTSLAGAGAHSTSASLATPI TTLGTIATLSSQVINPTAITVSAAQTTLTAAGGLTTPTITMQPVSQPTQVTLITAPSGVEAQPVHDLPVS ILASPTTEQPTATVTIADSGQGDVQPGTVTLVCSNPPCETHETGTTNTATTTVVANLGGHPQPTQVQFVC DRQETAASLVTSAVGQQNGNVVRVCSNPPCETHETGTTNTATTATSNMAGQHGCSNPPCETHETGTTSTA TTAMSSMGTGQQRDTRRTTNTPTVVRITVAPGALERVQGTVKPQCQTQQTNMTTTTMTVQATGAPCSAGP LLRPSVALESGSHSPAFVQLALPSVRVGLSGPSSKDMPTGRQPETYHTYTTNTPTTTRSIMVAGELGAAR VVPTSTYESLQASSPSSTMTMTALEALLCPSATVTQVCSNPPCETHETGTTNTATTSNAGSAQRVCSNPP CETHETGTTHTATTATSNGGAGQPEGGQQPASGHPCETHQTTSTGTTMSVSVGTLIPDATSSHGTLESGL EVVAVPTVTSQAGSTLLASFPTQRVCSNPPCETHETGTTHTATTVTSNMSSNQDPPPAASDQGEVASTQG DSTNITSASAITTSVSSTLPRAVTTVTQSTPVPGPSVPPPEELQVSPGPRQQLPPRQLLQSASTPLMGES TEVLSASQTPELQAAVDLSSTGDPSSGQEPTTSAVVATVVVQPPPPTQSEVDQLSLPQELMAEAQAGTTT LMVTGLTPEELAVTAAAEAAAQAAATEEAQALAIQAVLQAAQQAVMGTGEPMDTSEAAAAVTQAELGHLS AEGQEGQATTIPIVLTQQELAALVQQQQQLQEAQAQAQQQHHLPTEALAPADSLNDPSIESNCLNELASA VPSTVALLPSTATESLAPSNTFVAPQPVVASPAKMQAAATLTEVANGIESLGVKPDLPPPPSKAPVKKEN QWFDVGVIKGTSVMVTHYFLPPDDAVQSDDDSGTVPDYNQLKKQELQPGTAYKFRVAGINACGRGPFSEI SAFKTCLPGFPGAPCAIKISKSPDGAHLTWEPPSVTSGKIIEYSVYLAIQSSQASGEPKSSTPAQLAFMR VYCGPSPSCLVQSSSLSNAHIDYTTKPAIIFRIAARNEKGYGPATQVRWLQETSKDSSGTKPASKRPMSS PEMKSAPKKSKADGQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_008224
ORF Size	6135 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_008224.2
RefSeq Size	8093 bp
RefSeq ORF	6138 bp
Locus ID	15161
UniProt ID	Q61191
MW	210.9 kDa
Gene Summary	This gene encodes a transcription cofactor that regulates the progression of cell cycle and maintain the ability of embryonic stem cells to self-renew. The encoded protein is a large precursor that undergoes site-specific proteolytic cleavage to yield N- and C-terminal chains that form a non-covalent heterodimer. The encoded protein has been implicated in the regulation of expression of immediate early genes after herpes simplex virus infection and glucose-stimulated secretion of insulin by pancreatic beta cells. [provided by RefSeq, Aug 2015]

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