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Clasp1 (NM_029709) Mouse Tagged ORF Clone
CAT＃: MR216324
- TrueORF®
Clasp1 (Myc-DDK-tagged) - Mouse CLIP associating protein 1 (Clasp1), transcript variant 3
ORF Plasmid: tGFP
"NM_029709" in other vectors (4)
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CNY 14,540.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 1700030C23Rik; 5730583A19Rik; B130045P17Rik; CLASP1alpha |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR216324 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAACCGAGAATGGAGTCCTGTCTGGCCCAGGTGCTGCAGAAGGATGTGGGAAAGCGGCTGCAGGTTG GCCAGGAACTCATAGACTATTTCTCAGACAGACAGAAGTCTGCTGACCTTGAGCACGACCAGACCCTGTT GGATAAGCTTGTGGATGGACTCGCTACCTCTTGGGTGAACTCTAGCAATTACAAGGTGGTTCTCTTGGGC ATGGACATCCTGTCGGCACTGGTGACTAGGCTGCAGGACCGGTTCAAGGCGCAAATCGGCACAGTGTTGC CAAGTCTAATAGACAGACTGGGAGATGCTAAAGACTCCGTGAGGGAGCAAGACCAAACCCTGCTGCTAAA GATCATGGATCAAGCTGCTAATCCCCAGTATGTGTGGGACAGAATGCTCGGAGGCTTCAAACACAAGAAC TTCCGCACAAGAGAGGGCATCTGCCTCTGCCTTATTGCAACACTCAATGCCTCTGGGGCCCAGACTCTAA CACTAAGCAAGATTGTGCCACATATATGTAACCTACTGGGAGATCCCAACAGCCAGGTTCGAGACGCAGC AATAAACAGTCTGGTGGAGATTTATAGACATGTAGGTGAACGTGTGAGGGCAGACCTCAGTAAGAAAGGA CTGCCACAGTCCCGGTTGAATGTCATTTTTACAAAATTTGATGAAGTCCAAAAGTCTGGAAATATGATAC AGTCTGCAAATGAAAAAAATTTTGATGATGAAGATTCTGTGGATGGCAACAGGCCTTCTTCTGCCAGCTC CTCATCATCCAAGGCCCCATCAAGTTCCCGGAGGAATGTTAACCTGGGGACCACCCGTAGGCTCATGTCA TCCAGTCTTGGATCTAAGTCTTCAGCTGCAAAAGAAGGCGCTGGTGCTGTGGATGAAGAGGATTTTATTA AAGCCTTTGATGATGTACCTGTAGTGCAGATTTACTCCAGCCGAGACCTCGAGGAATCCATAAACAAAAT CAGAGAAATCCTGTCAGATGACAAGCATGACTGGGAGCAGAGAGTAAATGCTCTAAAAAAGATTAGATCG TTACTCTTGGCTGGGGCTGCTGAGTACGATAACTTCTTTCAACACTTGCGTCTTCTGGACGGGGCCTTTA AACTCTCTGCTAAGGACCTGCGGTCTCAGGTAGTGCGGGAAGCTTGTATCACATTGGGGCATCTGTCATC AGTTCTGGGAAATAAGTTTGATCATGGAGCTGAAGCCATTATGCCAACTATCTTTAATTTAATCCCGAAC AGTGCCAAAATTATGGCTACTTCTGGTGTTGTAGCTGTTAGGCTAATCATTCGGCACACTCACATCCCTC GGCTGATCCCTGTCATAACCAGCAACTGTACCTCTAAGTCTGTCGCCGTCAGAAGGCGCTGTTTTGAATT TTTAGATTTGCTTTTACAAGAATGGCAGACACATTCACTAGAAAGACATATATCAGTATTAGCTGAAACA ATAAAGAAAGGAATACACGACGCTGATTCTGAAGCGAGAATAGAAGCCAGGAAGTGCTACTGGGGTTTCC ACAGTCACTTCAGCCGAGAAGCAGAACACCTGTACCACACTTTGGAGTCCTCGTATCAGAAGGCCCTACA GTCCCACTTGAAGAACTCGGACAGCATCGTGTCTCTGCCCCAGTCAGACCGATCCTCTTCCAGCTCTCAA GAGAGTCTCAACCGGCCACTTTCAGCCAAAAGAAGTCCCACTGGCAGCACTGCATCCAGAGGCTCTACAG TTAGTACCAAATCTGTGTCGACGACAGGATCTCTCCAGCGATCTCGAAGTGATATTGATGTGAACGCAGC AGCCAGTGCCAAATCCAAAGTCTCCTCATCCTCAGGATCCCCCGCCTTCAGCTCTGCAGCAGCACTGCCC CCAGGATCCTATGCGTCCCTAGGTCGGATCCGCACGAGACGGCAGAGCTCGGGGAGCACCACCAATGTCG CCTCCACACCCTCCGACAGTCGGGGCCGCAGTCGCGCCAAAGTGGTTTCACAGTCTCAGCCTGGCAGCCG GTCAAGTTCCCCTGGGAAGCTTTTGGGAAGTGGACTTGCTGGGGGTTCCTCCAGAGGCCCACCGGTAACA CCATCCTCAGAAAAACGGAGCAAGATCCCCAGGAGTCAGGGATGTAGCCGAGAAACAAGTCCTAACCGGA TTGGATTAGATCGGTTTGGGCTGGGCCAGTCAGGACGAATCCCTGGTTCTGTGAACGCCATGAGAGTCTT GAGTACCAGCACTGACCTGGAAGCAGCAGTGGCTGACGCTCTGCTGTTAGGAGACGCCAGGAGCAAGAAG AAGCCTGTGAGAAGGAGATACGAGCCCTATGGAATGTACTCTGATGATGATGCCAACAGTGATGCCTCCA GCGTGTGCTCTGAGCGCTCATATGGCTCCAGGAATGGTGGCATTCCCCATTATCTGCGGCAGACTGAAGA TGTAGCAGAGGTTCTCAACCATTGTGCCAGTTCCAACTGGTCAGAACGGAAAGAGGGGCTCCTGGGCCTG CAGAACTTACTGAAGAGCCAAAGAACACTGAGTCGAGTAGAATTGAAGAGACTGTGTGAGATTTTCACCC GAATGTTTGCCGACCCTCACAGCAAGAGAGTTTTCAGTATGTTTTTGGAGACCCTCGTGGATTTTATAAT CATTCATAAGGATGACTTGCAAGACTGGCTCTTCGTCCTTCTCACACAACTACTTAAGAAAATGGGAGCA GACTTACTTGGATCTGTGCAAGCAAAAGTTCAGAAGGCTCTCGATGTCACCAGGGATTCCTTTCCATTTG ATCAACAATTTAACATTTTGATGAGATTTATTGTGGATCAGACTCAAACTCCAAACCTCAAGGTCAAAGT AGCAATCCTGAAATACATCGAATCTCTAGCCAGACAGATGGACCCCACAGATTTTGTAAATTCCAGCGAA ACAAGACTAGCTGTTTCTAGGATCATAACATGGACTACAGAACCAAAGAGTTCAGACGTGAGAAAGGCAG CACAAATTGTGCTCATCTCTCTGTTTGAACTGAACACGCCTGAATTTACCATGTTACTTGGTGCCTTGCC AAAAACATTCCAGGATGGTGCCACCAAACTCCTGCATAACCACCTCAAGAACTCCAGTAACACCGGTGTG GGATCTCCAAGCAATACAATTGGCCGGACACCTTCCCGCCACCCCAGCAGCAGGACCAGCCCCCTGACCT CACCCACCAACTGTTCCCATGGGGGACTATCTCCAAGCATGCTGGACTATGATACAGAGAACCTGAACTC TGAAGAAATCTACAGCTCTTTGCGTGGAGTTACAGAAGCCATTGAAAAGTTCAGCTTCCGAAGCCAGGAG GATCTAAATGAGCCAATCAAACGAGATGGCAAGAAGGATTGTGATATCGTGTCCCGAGATGGGGGAGCAG CCTCACCTGCCACCGAGGGCCGGGGAGGTAGTGAGATAGAAGGAGGCAGGATGGCTTTGGACAACAAGAC CTCCCTGCTCAACACGCAGCCTCCACGTGCCTTTCCGGGGCCAAGAGCACGGGAATATAACCCGTATCCC TACTCCGACACCATCAACACCTATGACAAGACGGCTCTGAAGGAAGCAGTGTTTGACGATGACATGGAGC AGCTCCGAGATGTGCCCATTGACCACTCAGACCTGGTGGCTGACTTGCTGAAAGAGCTATCTAACCACAA CGAGCGTGTGGAGGAGCGGAAGGGCGCACTGCTGGAGTTGCTCAAGATCACCAGGGAGGACAGCCTGGGC GTGTGGGAGGAGCACTTCAAGACCATCCTGCTGCTGCTGCTGGAAACTCTCGGAGACAAAGACCATTCCA TTCGAGCTCTGGCACTGAGAGTTTTACGGGAAATTCTGAGAAACCAGCCAGCAAGATTCAAAAACTATGC AGAACTGACGATCATGAAGACTCTGGAAGCCCACAAAGACTCCCACAAGGAGGTGGTGAGAGCGGCCGAG GAAGCTGCATCCACGCTAGCCAGCTCAATCCACCCAGAGCAGTGCATCAAAGTGCTGTGTCCAATCATCC AGACAGCCGACTACCCCATCAACCTGGCTGCTATCAAGATGCAGACAAAGGTGGTGGAGAGGATCACCAA GGAGTCCTTGCTGCAGCTCCTCGTCGACATCATCCCCGGCCTGCTGCAGGGTTACGACAACACCGAGAGC AGTGTACGGAAAGCCAGTGTGTTTTGCTTAGTGGCAATCTATTCCGTAATCGGAGAAGATCTGAAACCTC ACCTCGCACAGCTCACGGGGAGCAAGATGAAGCTGCTGAACTTATATATAAAGAGGGCCCAGACTACCAA CAGCAACAGCAGCTCTTCCTCTGATGTGTCCACACACAGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR216324 protein sequence
Red=Cloning site Green=Tags(s) MEPRMESCLAQVLQKDVGKRLQVGQELIDYFSDRQKSADLEHDQTLLDKLVDGLATSWVNSSNYKVVLLG MDILSALVTRLQDRFKAQIGTVLPSLIDRLGDAKDSVREQDQTLLLKIMDQAANPQYVWDRMLGGFKHKN FRTREGICLCLIATLNASGAQTLTLSKIVPHICNLLGDPNSQVRDAAINSLVEIYRHVGERVRADLSKKG LPQSRLNVIFTKFDEVQKSGNMIQSANEKNFDDEDSVDGNRPSSASSSSSKAPSSSRRNVNLGTTRRLMS SSLGSKSSAAKEGAGAVDEEDFIKAFDDVPVVQIYSSRDLEESINKIREILSDDKHDWEQRVNALKKIRS LLLAGAAEYDNFFQHLRLLDGAFKLSAKDLRSQVVREACITLGHLSSVLGNKFDHGAEAIMPTIFNLIPN SAKIMATSGVVAVRLIIRHTHIPRLIPVITSNCTSKSVAVRRRCFEFLDLLLQEWQTHSLERHISVLAET IKKGIHDADSEARIEARKCYWGFHSHFSREAEHLYHTLESSYQKALQSHLKNSDSIVSLPQSDRSSSSSQ ESLNRPLSAKRSPTGSTASRGSTVSTKSVSTTGSLQRSRSDIDVNAAASAKSKVSSSSGSPAFSSAAALP PGSYASLGRIRTRRQSSGSTTNVASTPSDSRGRSRAKVVSQSQPGSRSSSPGKLLGSGLAGGSSRGPPVT PSSEKRSKIPRSQGCSRETSPNRIGLDRFGLGQSGRIPGSVNAMRVLSTSTDLEAAVADALLLGDARSKK KPVRRRYEPYGMYSDDDANSDASSVCSERSYGSRNGGIPHYLRQTEDVAEVLNHCASSNWSERKEGLLGL QNLLKSQRTLSRVELKRLCEIFTRMFADPHSKRVFSMFLETLVDFIIIHKDDLQDWLFVLLTQLLKKMGA DLLGSVQAKVQKALDVTRDSFPFDQQFNILMRFIVDQTQTPNLKVKVAILKYIESLARQMDPTDFVNSSE TRLAVSRIITWTTEPKSSDVRKAAQIVLISLFELNTPEFTMLLGALPKTFQDGATKLLHNHLKNSSNTGV GSPSNTIGRTPSRHPSSRTSPLTSPTNCSHGGLSPSMLDYDTENLNSEEIYSSLRGVTEAIEKFSFRSQE DLNEPIKRDGKKDCDIVSRDGGAASPATEGRGGSEIEGGRMALDNKTSLLNTQPPRAFPGPRAREYNPYP YSDTINTYDKTALKEAVFDDDMEQLRDVPIDHSDLVADLLKELSNHNERVEERKGALLELLKITREDSLG VWEEHFKTILLLLLETLGDKDHSIRALALRVLREILRNQPARFKNYAELTIMKTLEAHKDSHKEVVRAAE EAASTLASSIHPEQCIKVLCPIIQTADYPINLAAIKMQTKVVERITKESLLQLLVDIIPGLLQGYDNTES SVRKASVFCLVAIYSVIGEDLKPHLAQLTGSKMKLLNLYIKRAQTTNSNSSSSSDVSTHS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_029709 |
| ORF Size | 4380 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_029709.2, NP_083985.2 |
| RefSeq Size | 7675 bp |
| RefSeq ORF | 4383 bp |
| Locus ID | 76707 |
| UniProt ID | Q80TV8 |
| MW | 161.1 kDa |
| Gene Summary | Microtubule plus-end tracking protein that promotes the stabilization of dynamic microtubules. Involved in the nucleation of noncentrosomal microtubules originating from the trans-Golgi network (TGN). Required for the polarization of the cytoplasmic microtubule arrays in migrating cells towards the leading edge of the cell. May act at the cell cortex to enhance the frequency of rescue of depolymerizing microtubules by attaching their plus-ends to cortical platforms composed of ERC1 and PHLDB2. This cortical microtubule stabilizing activity is regulated at least in part by phosphatidylinositol 3-kinase signaling. Also performs a similar stabilizing function at the kinetochore which is essential for the bipolar alignment of chromosomes on the mitotic spindle (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC224501 | Clasp1 (untagged) - Mouse CLIP associating protein 1 (Clasp1), transcript variant 3, (10ug) |
CNY 18,720.00 |
|
| MG216324 | Clasp1 (tGFP-tagged) - Mouse CLIP associating protein 1 (Clasp1) transcript variant 3, (10ug) |
CNY 15,960.00 |
|
| MR216324L3 | Lenti ORF clone of Clasp1 (Myc-DDK-tagged) - Mouse CLIP associating protein 1 (Clasp1), transcript variant 3 |
CNY 16,440.00 |
|
| MR216324L4 | Lenti ORF clone of Clasp1 (mGFP-tagged) - Mouse CLIP associating protein 1 (Clasp1), transcript variant 3 |
CNY 16,440.00 |
