Ly75 (NM_013825) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	CD205; DEC-205; DEC205
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR217172 representing NM_013825 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGGACGGGCCGGGTGACCCCGGGCCTGGCGGCGGGGCTACTCCTGCTGTTGCTGCGGTCCTTCGGGC TTGTGGAGCCTTCTGAGAGCTCAGGTAATGATCCATTCACCATCGTCCATGAAAACACTGGCAAGTGCAT CCAGCCGCTGTCTGACTGGGTAGTGGCCCAGGACTGTAGCGGAACTAACAACATGTTGTGGAAGTGGGTG TCCCAGCACCGCCTCTTTCACCTGGAATCCCAGAAGTGCCTCGGCCTCGATATTACCAAAGCCACGGACA ACCTGCGAATGTTCAGCTGTGACTCCACCGTCATGCTGTGGTGGAAATGTGAGCACCATTCGCTGTACAC CGCTGCCCAGTACAGGCTAGCTCTGAAAGATGGATATGCCGTAGCCAATACGAATACATCTGATGTCTGG AAGAAGGGAGGCTCCGAGGAAAACCTTTGTGCCCAGCCTTATCATGAGATATACACCAGAGATGGGAATT CCTACGGGAGACCTTGTGAATTCCCTTTCTTGATTGGTGAGACATGGTACCATGACTGCATTCATGATGA AGATCATAGTGGGCCATGGTGTGCCACTACCCTAAGTTATGAATATGATCAAAAGTGGGGCATCTGCCTA CTACCAGAAAGTGGCTGTGAAGGTAACTGGGAAAAGAATGAGCAGATTGGAAGTTGCTACCAATTTAATA ATCAGGAAATTCTGTCTTGGAAAGAAGCTTATGTTTCCTGTCAGAACCAAGGAGCTGACTTACTGAGCAT CCACAGTGCTGCCGAATTAGCCTACATTACGGGAAAAGAGGACATTGCTAGACTTGTTTGGCTTGGACTG AATCAGCTCTATTCTGCGAGAGGTTGGGAATGGTCAGACTTCAGGCCACTCAAATTTCTTAACTGGGATC CAGGCACGCCCGTTGCACCTGTGATTGGTGGGTCAAGCTGTGCCAGAATGGACACAGAGTCCGGGCTGTG GCAAAGTGTTTCCTGTGAATCTCAGCAGCCTTACGTCTGCAAGAAGCCACTGAACAACACGCTGGAGCTC CCAGATGTTTGGACTTACACAGATACCCACTGCCATGTGGGCTGGCTGCCAAATAATGGGTTTTGCTATC TGCTGGCGAATGAAAGTAGTTCCTGGGATGCAGCACATTTGAAATGCAAAGCCTTCGGTGCAGACCTCAT CAGCATGCACTCCTTAGCAGATGTGGAGGTGGTTGTCACGAAACTCCATAATGGGGATGTCAAAAAAGAA ATATGGACAGGCCTTAAAAACACAAACAGCCCTGCTTTGTTCCAGTGGTCGGACGGAACGGAAGTTACTC TAACGTACTGGAATGAGAATGAGCCGAGTGTTCCCTTCAACAAGACTCCCAACTGTGTTTCCTATTTAGG AAAGTTAGGTCAGTGGAAAGTCCAGTCCTGTGAGAAGAAACTCAGATATGTATGCAAGAAAAAGGGAGAA ATAACTAAGGATGCAGAGTCGGATAAGCTGTGTCCGCCAGACGAGGGCTGGAAGAGACATGGAGAAACCT GTTACAAGATTTATGAGAAAGAGGCCCCTTTCGGAACGAACTGCAACCTGACCATCACTAGCAGGTTCGA GCAGGAATTCTTGAATTATATGATGAAGAACTATGATAAGTCCCTTCGGAAGTACTTCTGGACTGGCCTG AGAGACCCTGACTCTCGAGGAGAATACAGTTGGGCCGTTGCTCAGGGAGTAAAGCAGGCTGTGACCTTTT CCAACTGGAATTTTCTTGAACCGGCGTCTCCAGGCGGGTGCGTGGCTATGTCTACTGGAAAGACTCTTGG CAAGTGGGAAGTGAAGAACTGCAGAAGCTTCCGTGCTCTTTCAATATGCAAGAAAGTGAGCGAACCCCAG GAGCCTGAAGAAGCAGCCCCCAAGCCCGACGACCCCTGTCCTGAAGGCTGGCACACTTTCCCCTCCAGCC TTTCTTGTTATAAGGTGTTCCATATAGAAAGAATCGTAAGAAAGAGGAACTGGGAAGAAGCCGAAAGGTT CTGCCAAGCCCTTGGAGCTCACCTACCCAGCTTCAGTCGTAGAGAGGAAATTAAGGACTTTGTGCATTTG TTAAAGGACCAGTTCAGTGGGCAGCGTTGGTTGTGGATTGGTCTGAATAAGAGAAGCCCTGATTTACAAG GGTCCTGGCAGTGGAGTGACCGGACACCAGTGTCTGCTGTGATGATGGAGCCGGAGTTTCAACAGGATTT TGACATCAGAGACTGTGCTGCCATCAAGGTCCTTGATGTACCTTGGCGAAGAGTCTGGCATCTCTATGAG GACAAGGACTATGCTTACTGGAAACCTTTTGCTTGTGATGCCAAGCTTGAGTGGGTGTGCCAGATTCCAA AAGGTAGCACTCCCCAGATGCCAGACTGGTATAATCCAGAGCGCACTGGAATTCATGGGCCCCCAGTTAT AATTGAAGGAAGTGAATACTGGTTTGTTGCTGATCCCCACTTAAACTACGAAGAAGCCGTCTTATACTGT GCTAGCAATCACAGCTTTCTTGCCACGATAACATCGTTCACAGGACTAAAAGCTATCAAAAACAAACTAG CAAATATTTCTGGCGAGGAACAGAAGTGGTGGGTGAAAACGAGTGAGAATCCAATTGATCGTTACTTTCT AGGCTCGCGCCGCCGCCTGTGGCACCATTTCCCCATGACGTTTGGAGATGAATGTTTGCACATGTCAGCC AAGACGTGGCTTGTTGACTTAAGTAAACGAGCGGACTGTAATGCCAAGTTGCCCTTCATCTGTGAAAGAT ACAATGTCTCTTCATTAGAGAAATACAGCCCAGATCCTGCAGCCAAAGTACAGTGCACTGAGAAGTGGAT TCCTTTTCAAAATAAGTGCTTCCTAAAGGTCAACTCTGGGCCCGTTACGTTTTCTCAAGCAAGCGGCATT TGTCATTCCTACGGCGGCACCCTTCCTTCCGTGCTGAGCCGGGGTGAACAAGATTTCATTATATCCTTGC TTCCTGAAATGGAAGCTAGTCTATGGATTGGTCTGCGCTGGACTGCCTACGAAAGGATAAACAGATGGAC AGACAACAGAGAGCTGACCTACAGCAACTTTCACCCACTGCTGGTCGGTCGGAGGCTGAGCATACCAACG AATTTCTTTGATGATGAGTCCCACTTCCACTGCGCCTTGATTCTTAATCTCAAAAAGTCACCGCTTACTG GGACCTGGAATTTTACTTCCTGTTCAGAACGACACTCTCTGTCTCTCTGTCAAAAATACTCAGAGACTGA AGACGGACAGCCCTGGGAGAACACTTCAAAAACAGTGAAGTATCTAAATAACCTATACAAAATCATCTCG AAGCCCCTGACGTGGCACGGCGCTCTGAAGGAGTGCATGAAAGAGAAGATGAGGTTGGTGAGCATCACAG ACCCTTACCAGCAGGCCTTCCTCGCAGTGCAGGCCACCCTGCGCAACAGCTCCTTCTGGATCGGACTCTC CAGTCAAGATGATGAACTCAACTTTGGTTGGTCAGATGGGAAACGTCTTCAATTTAGTAACTGGGCTGGA AGCAATGAGCAACTTGATGACTGCGTGATATTAGACACAGATGGATTCTGGAAAACAGCTGACTGTGATG ATAACCAGCCTGGCGCCATTTGCTACTATCCAGGAAATGAGACTGAGGAGGAGGTCAGAGCACTGGACAC TGCTAAATGCCCGTCTCCTGTACAGAGCACCCCATGGATACCATTCCAGAACTCCTGCTACAATTTCATG ATTACCAACAACAGGCATAAGACAGTCACACCGGAGGAAGTGCAGTCCACGTGCGAGAAGCTGCATTCGA AAGCACACAGTCTGAGCATTCGGAATGAGGAGGAGAATACCTTTGTTGTGGAACAGCTTCTGTACTTCAA TTATATTGCCTCATGGGTCATGTTAGGAATAACCTATGAAAACAATTCTTTGATGTGGTTTGATAAAACT GCATTGTCCTACACACACTGGAGAACGGGAAGACCAACTGTGAAAAATGGCAAATTTTTGGCTGGTCTAA GTACTGATGGATTCTGGGATATTCAGTCTTTCAATGTTATTGAAGAAACACTTCATTTTTACCAGCACAG TATTTCTGCTTGTAAAATTGAAATGGTTGACTATGAGGACAAACACAATGGCACCCTGCCACAGTTCATT CCATATAAGGACGGCGTCTACAGCGTTATTCAGAAGAAGGTGACGTGGTATGAAGCATTGAACGCGTGCT CTCAAAGTGGGGGAGAGTTGGCCAGTGTTCACAACCCAAATGGGAAGCTCTTTCTGGAAGACATTGTGAA CCGTGACGGATTCCCTCTCTGGGTTGGGCTCTCAAGTCATGATGGAAGCGAATCGAGTTTCGAATGGTCC GATGGCAGAGCATTTGACTATGTCCCATGGCAGAGCCTACAATCTCCCGGAGACTGTGTCGTCTTATATC CAAAAGGAATTTGGAGACGTGAAAAATGCCTGTCTGTTAAGGATGGTGCTATTTGTTACAAGCCTACAAA AGATAAAAAGCTGATCTTTCATGTAAAATCATCAAAATGTCCAGTGGCAAAGAGGGATGGTCCCCAGTGG GTCCAGTATGGGGGCCACTGTTACGCTTCGGACCAGGTACTGCACAGCTTCTCAGAGGCCAAACAAGTGT GTCAAGAGCTTGATCATTCGGCAACTGTTGTCACCATAGCAGATGAAAATGAGAATAAGTTTGTGAGCAG ACTGATGAGGGAGAACTATAATATTACTATGAGAGTTTGGCTTGGCCTGTCTCAGCATTCACTCGATCAG TCTTGGAGTTGGCTCGATGGATTAGATGTGACATTTGTCAAATGGGAAAATAAAACTAAGGATGGTGATG GGAAATGTAGCATTTTAATAGCTTCAAATGAAACCTGGAGAAAAGTCCATTGCTCACGTGGCTATGCAAG AGCTGTCTGCAAAATTCCTCTGAGCCCGGACTACACAGGCATAGCCATCCTGTTTGCCGTGCTGTGCCTC TTAGGGCTCATCAGCTTGGCGATTTGGTTCCTCTTGCAACGATCCCATATCCGCTGGACCGGCTTCTCCT CGGTTCGGTATGAACATGGAACCAACGAAGACGAGGTGATGCTCCCTTCTTTCCACGAC AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR217172 representing NM_013825 Red=Cloning site Green=Tags(s) MRTGRVTPGLAAGLLLLLLRSFGLVEPSESSGNDPFTIVHENTGKCIQPLSDWVVAQDCSGTNNMLWKWV SQHRLFHLESQKCLGLDITKATDNLRMFSCDSTVMLWWKCEHHSLYTAAQYRLALKDGYAVANTNTSDVW KKGGSEENLCAQPYHEIYTRDGNSYGRPCEFPFLIGETWYHDCIHDEDHSGPWCATTLSYEYDQKWGICL LPESGCEGNWEKNEQIGSCYQFNNQEILSWKEAYVSCQNQGADLLSIHSAAELAYITGKEDIARLVWLGL NQLYSARGWEWSDFRPLKFLNWDPGTPVAPVIGGSSCARMDTESGLWQSVSCESQQPYVCKKPLNNTLEL PDVWTYTDTHCHVGWLPNNGFCYLLANESSSWDAAHLKCKAFGADLISMHSLADVEVVVTKLHNGDVKKE IWTGLKNTNSPALFQWSDGTEVTLTYWNENEPSVPFNKTPNCVSYLGKLGQWKVQSCEKKLRYVCKKKGE ITKDAESDKLCPPDEGWKRHGETCYKIYEKEAPFGTNCNLTITSRFEQEFLNYMMKNYDKSLRKYFWTGL RDPDSRGEYSWAVAQGVKQAVTFSNWNFLEPASPGGCVAMSTGKTLGKWEVKNCRSFRALSICKKVSEPQ EPEEAAPKPDDPCPEGWHTFPSSLSCYKVFHIERIVRKRNWEEAERFCQALGAHLPSFSRREEIKDFVHL LKDQFSGQRWLWIGLNKRSPDLQGSWQWSDRTPVSAVMMEPEFQQDFDIRDCAAIKVLDVPWRRVWHLYE DKDYAYWKPFACDAKLEWVCQIPKGSTPQMPDWYNPERTGIHGPPVIIEGSEYWFVADPHLNYEEAVLYC ASNHSFLATITSFTGLKAIKNKLANISGEEQKWWVKTSENPIDRYFLGSRRRLWHHFPMTFGDECLHMSA KTWLVDLSKRADCNAKLPFICERYNVSSLEKYSPDPAAKVQCTEKWIPFQNKCFLKVNSGPVTFSQASGI CHSYGGTLPSVLSRGEQDFIISLLPEMEASLWIGLRWTAYERINRWTDNRELTYSNFHPLLVGRRLSIPT NFFDDESHFHCALILNLKKSPLTGTWNFTSCSERHSLSLCQKYSETEDGQPWENTSKTVKYLNNLYKIIS KPLTWHGALKECMKEKMRLVSITDPYQQAFLAVQATLRNSSFWIGLSSQDDELNFGWSDGKRLQFSNWAG SNEQLDDCVILDTDGFWKTADCDDNQPGAICYYPGNETEEEVRALDTAKCPSPVQSTPWIPFQNSCYNFM ITNNRHKTVTPEEVQSTCEKLHSKAHSLSIRNEEENTFVVEQLLYFNYIASWVMLGITYENNSLMWFDKT ALSYTHWRTGRPTVKNGKFLAGLSTDGFWDIQSFNVIEETLHFYQHSISACKIEMVDYEDKHNGTLPQFI PYKDGVYSVIQKKVTWYEALNACSQSGGELASVHNPNGKLFLEDIVNRDGFPLWVGLSSHDGSESSFEWS DGRAFDYVPWQSLQSPGDCVVLYPKGIWRREKCLSVKDGAICYKPTKDKKLIFHVKSSKCPVAKRDGPQW VQYGGHCYASDQVLHSFSEAKQVCQELDHSATVVTIADENENKFVSRLMRENYNITMRVWLGLSQHSLDQ SWSWLDGLDVTFVKWENKTKDGDGKCSILIASNETWRKVHCSRGYARAVCKIPLSPDYTGIAILFAVLCL LGLISLAIWFLLQRSHIRWTGFSSVRYEHGTNEDEVMLPSFHD SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_013825
ORF Size	5169 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_013825.4
RefSeq Size	5172 bp
RefSeq ORF	5172 bp
Locus ID	17076
UniProt ID	Q60767
MW	197.8 kDa
Gene Summary	Acts as an endocytic receptor to direct captured antigens from the extracellular space to a specialized antigen-processing compartment. Causes reduced proliferation of B lymphocytes (By similarity).[UniProtKB/Swiss-Prot Function]

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