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Clasp1 (NM_177548) Mouse Tagged ORF Clone
CAT＃: MR217833
- TrueORF®
Clasp1 (Myc-DDK-tagged) - Mouse CLIP associating protein 1 (Clasp1), transcript variant 2
ORF Plasmid: tGFP
"NM_177548" in other vectors (4)
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CNY 14,630.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 1700030C23Rik; 5730583A19Rik; B130045P17Rik; mKIAA0622 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR217833 representing NM_177548
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAACCGAGAATGGAGTCCTGTCTGGCCCAGGTGCTGCAGAAGGATGTGGGAAAGCGGCTGCAGGTTG GCCAGGAACTCATAGACTATTTCTCAGACAGACAGAAGTCTGCTGACCTTGAGCACGACCAGACCCTGTT GGATAAGCTTGTGGATGGACTCGCTACCTCTTGGGTGAACTCTAGCAATTACAAGGTGGTTCTCTTGGGC ATGGACATCCTGTCGGCACTGGTGACTAGGCTGCAGGACCGGTTCAAGGCGCAAATCGGCACAGTGTTGC CAAGTCTAATAGACAGACTGGGAGATGCTAAAGACTCCGTGAGGGAGCAAGACCAAACCCTGCTGCTAAA GATCATGGATCAAGCTGCTAATCCCCAGTATGTGTGGGACAGAATGCTCGGAGGCTTCAAACACAAGAAC TTCCGCACAAGAGAGGGCATCTGCCTCTGCCTTATTGCAACACTCAATGCCTCTGGGGCCCAGACTCTAA CACTAAGCAAGATTGTGCCACATATATGTAACCTACTGGGAGATCCCAACAGCCAGGTTCGAGACGCAGC AATAAACAGTCTGGTGGAGATTTATAGACATGTAGGTGAACGTGTGAGGGCAGACCTCAGTAAGAAAGGA CTGCCACAGTCCCGGTTGAATGTCATTTTTACAAAATTTGATGAAGTCCAAAAGTCTGGAAATATGATAC AGTCTGCAAATGAAAAAAATTTTGATGATGAAGATTCTGTGGATGGCAACAGGCCTTCTTCTGCCAGCTC CTCATCATCCAAGGCCCCATCAAGTTCCCGGAGGAATGTTAACCTGGGGACCACCCGTAGGCTCATGTCA TCCAGTCTTGGATCTAAGTCTTCAGCTGCAAAAGAAGGCGCTGGTGCTGTGGATGAAGAGGATTTTATTA AAGCCTTTGATGATGTACCTGTAGTGCAGATTTACTCCAGCCGAGACCTCGAGGAATCCATAAACAAAAT CAGAGAAATCCTGTCAGATGACAAGCATGACTGGGAGCAGAGAGTAAATGCTCTAAAAAAGATTAGATCG TTACTCTTGGCTGGGGCTGCTGAGTACGATAACTTCTTTCAACACTTGCGTCTTCTGGACGGGGCCTTTA AACTCTCTGCTAAGGACCTGCGGTCTCAGGTAGTGCGGGAAGCTTGTATCACATTGGGGCATCTGTCATC AGTTCTGGGAAATAAGTTTGATCATGGAGCTGAAGCCATTATGCCAACTATCTTTAATTTAATCCCGAAC AGTGCCAAAATTATGGCTACTTCTGGTGTTGTAGCTGTTAGGCTAATCATTCGGCACACTCACATCCCTC GGCTGATCCCTGTCATAACCAGCAACTGTACCTCTAAGTCTGTCGCCGTCAGAAGGCGCTGTTTTGAATT TTTAGATTTGCTTTTACAAGAATGGCAGACACATTCACTAGAAAGACATATATCAGTATTAGCTGAAACA ATAAAGAAAGGAATACACGACGCTGATTCTGAAGCGAGAATAGAAGCCAGGAAGTGCTACTGGGGTTTCC ACAGTCACTTCAGCCGAGAAGCAGAACACCTGTACCACACTTTGGAGTCCTCGTATCAGAAGGCCCTACA GTCCCACTTGAAGAACTCGGACAGCATCGTGTCTCTGCCCCAGTCAGACCGATCCTCTTCCAGCTCTCAA GAGAGTCTCAACCGGCCACTTTCAGCCAAAAGAAGTCCCACTGGCAGCACTGCATCCAGAGGCTCTACAG TTAGTACCAAATCTGTGTCGACGACAGGATCTCTCCAGCGATCTCGAAGTGATATTGATGTGAACGCAGC AGCCAGTGCCAAATCCAAAGTCTCCTCATCCTCAGGATCCCCCGCCTTCAGCTCTGCAGCAGCACTGCCC CCAGGATCCTATGCGTCCCTAGAGTCCAGACACATGAGGGAAGACTTGGAGTACGTAGGCCTGGATGCAG GTCGGATCCGCACGAGACGGCAGAGCTCGGGGAGCACCACCAATGTCGCCTCCACACCCTCCGACAGTCG GGGCCGCAGTCGCGCCAAAGTGGTTTCACAGTCTCAGCCTGGCAGCCGGTCAAGTTCCCCTGGGAAGCTT TTGGGAAGTGGACTTGCTGGGGGTTCCTCCAGAGGCCCACCGGTAACACCATCCTCAGAAAAACGGAGCA AGATCCCCAGGAGTCAGGGATGTAGCCGAGAAACAAGTCCTAACCGGATTGGATTAGATCGGTTTGGGCT GGGCCAGTCAGGACGAATCCCTGGTTCTGTGAACGCCATGAGAGTCTTGAGTACCAGCACTGACCTGGAA GCAGCAGTGGCTGACGCTCTGAAGAAGCCTGTGAGAAGGAGATACGAGCCCTATGGAATGTACTCTGATG ATGATGCCAACAGTGATGCCTCCAGCGTGTGCTCTGAGCGCTCATATGGCTCCAGGAATGGTGGCATTCC CCATTATCTGCGGCAGACTGAAGATGTAGCAGAGGTTCTCAACCATTGTGCCAGTTCCAACTGGTCAGAA CGGAAAGAGGGGCTCCTGGGCCTGCAGAACTTACTGAAGAGCCAAAGAACACTGAGTCGAGTAGAATTGA AGAGACTGTGTGAGATTTTCACCCGAATGTTTGCCGACCCTCACAGCAAGAGAGTTTTCAGTATGTTTTT GGAGACCCTCGTGGATTTTATAATCATTCATAAGGATGACTTGCAAGACTGGCTCTTCGTCCTTCTCACA CAACTACTTAAGAAAATGGGAGCAGACTTACTTGGATCTGTGCAAGCAAAAGTTCAGAAGGCTCTCGATG TCACCAGGGATTCCTTTCCATTTGATCAACAATTTAACATTTTGATGAGATTTATTGTGGATCAGACTCA AACTCCAAACCTCAAGGTCAAAGTAGCAATCCTGAAATACATCGAATCTCTAGCCAGACAGATGGACCCC ACAGATTTTGTAAATTCCAGCGAAACAAGACTAGCTGTTTCTAGGATCATAACATGGACTACAGAACCAA AGAGTTCAGACGTGAGAAAGGCAGCACAAATTGTGCTCATCTCTCTGTTTGAACTGAACACGCCTGAATT TACCATGTTACTTGGTGCCTTGCCAAAAACATTCCAGGATGGTGCCACCAAACTCCTGCATAACCACCTC AAGAACTCCAGTAACACCGGTGTGGGATCTCCAAGCAATACAATTGGCCGGACACCTTCCCGCCACCCCA GCAGCAGGACCAGCCCCCTGACCTCACCCACCAACTGTTCCCATGGGGGACTATCTCCAAGCATGCTGGA CTATGATACAGAGAACCTGAACTCTGAAGAAATCTACAGCTCTTTGCGTGGAGTTACAGAAGCCATTGAA AAGTTCAGCTTCCGAAGCCAGGAGGATCTAAATGAGCCAATCAAACGAGATGGCAAGAAGGATTGTGATA TCGTGTCCCGAGATGGGGGAGCAGCCTCACCTGCCACCGAGGGCCGGGGAGGTAGTGAGATAGAAGGAGG CAGGATGGCTTTGGACAACAAGACCTCCCTGCTCAACACGCAGCCTCCACGTGCCTTTCCGGGGCCAAGA GCACGGGAATATAACCCGTATCCCTACTCCGACACCATCAACACCTATGACAAGACGGCTCTGAAGGAAG CAGTGTTTGACGATGACATGGAGCAGCTCCGAGATGTGCCCATTGACCACTCAGACCTGGTGGCTGACTT GCTGAAAGAGCTATCTAACCACAACGAGCGTGTGGAGGAGCGGAAGGGCGCACTGCTGGAGTTGCTCAAG ATCACCAGGGAGGACAGCCTGGGCGTGTGGGAGGAGCACTTCAAGACCATCCTGCTGCTGCTGCTGGAAA CTCTCGGAGACAAAGACCATTCCATTCGAGCTCTGGCACTGAGAGTTTTACGGGAAATTCTGAGAAACCA GCCAGCAAGATTCAAAAACTATGCAGAACTGACGATCATGAAGACTCTGGAAGCCCACAAAGACTCCCAC AAGGAGGTGGTGAGAGCGGCCGAGGAAGCTGCATCCACGCTAGCCAGCTCAATCCACCCAGAGCAGTGCA TCAAAGTGCTGTGTCCAATCATCCAGACAGCCGACTACCCCATCAACCTGGCTGCTATCAAGATGCAGAC AAAGGTGGTGGAGAGGATCACCAAGGAGTCCTTGCTGCAGCTCCTCGTCGACATCATCCCCGGCCTGCTG CAGGGTTACGACAACACCGAGAGCAGTGTACGGAAAGCCAGTGTGTTTTGCTTAGTGGCAATCTATTCCG TAATCGGAGAAGATCTGAAACCTCACCTCGCACAGCTCACGGGGAGCAAGATGAAGCTGCTGAACTTATA TATAAAGAGGGCCCAGACTACCAACAGCAACAGCAGCTCTTCCTCTGATGTGTCCACACACAGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR217833 representing NM_177548
Red=Cloning site Green=Tags(s) MEPRMESCLAQVLQKDVGKRLQVGQELIDYFSDRQKSADLEHDQTLLDKLVDGLATSWVNSSNYKVVLLG MDILSALVTRLQDRFKAQIGTVLPSLIDRLGDAKDSVREQDQTLLLKIMDQAANPQYVWDRMLGGFKHKN FRTREGICLCLIATLNASGAQTLTLSKIVPHICNLLGDPNSQVRDAAINSLVEIYRHVGERVRADLSKKG LPQSRLNVIFTKFDEVQKSGNMIQSANEKNFDDEDSVDGNRPSSASSSSSKAPSSSRRNVNLGTTRRLMS SSLGSKSSAAKEGAGAVDEEDFIKAFDDVPVVQIYSSRDLEESINKIREILSDDKHDWEQRVNALKKIRS LLLAGAAEYDNFFQHLRLLDGAFKLSAKDLRSQVVREACITLGHLSSVLGNKFDHGAEAIMPTIFNLIPN SAKIMATSGVVAVRLIIRHTHIPRLIPVITSNCTSKSVAVRRRCFEFLDLLLQEWQTHSLERHISVLAET IKKGIHDADSEARIEARKCYWGFHSHFSREAEHLYHTLESSYQKALQSHLKNSDSIVSLPQSDRSSSSSQ ESLNRPLSAKRSPTGSTASRGSTVSTKSVSTTGSLQRSRSDIDVNAAASAKSKVSSSSGSPAFSSAAALP PGSYASLESRHMREDLEYVGLDAGRIRTRRQSSGSTTNVASTPSDSRGRSRAKVVSQSQPGSRSSSPGKL LGSGLAGGSSRGPPVTPSSEKRSKIPRSQGCSRETSPNRIGLDRFGLGQSGRIPGSVNAMRVLSTSTDLE AAVADALKKPVRRRYEPYGMYSDDDANSDASSVCSERSYGSRNGGIPHYLRQTEDVAEVLNHCASSNWSE RKEGLLGLQNLLKSQRTLSRVELKRLCEIFTRMFADPHSKRVFSMFLETLVDFIIIHKDDLQDWLFVLLT QLLKKMGADLLGSVQAKVQKALDVTRDSFPFDQQFNILMRFIVDQTQTPNLKVKVAILKYIESLARQMDP TDFVNSSETRLAVSRIITWTTEPKSSDVRKAAQIVLISLFELNTPEFTMLLGALPKTFQDGATKLLHNHL KNSSNTGVGSPSNTIGRTPSRHPSSRTSPLTSPTNCSHGGLSPSMLDYDTENLNSEEIYSSLRGVTEAIE KFSFRSQEDLNEPIKRDGKKDCDIVSRDGGAASPATEGRGGSEIEGGRMALDNKTSLLNTQPPRAFPGPR AREYNPYPYSDTINTYDKTALKEAVFDDDMEQLRDVPIDHSDLVADLLKELSNHNERVEERKGALLELLK ITREDSLGVWEEHFKTILLLLLETLGDKDHSIRALALRVLREILRNQPARFKNYAELTIMKTLEAHKDSH KEVVRAAEEAASTLASSIHPEQCIKVLCPIIQTADYPINLAAIKMQTKVVERITKESLLQLLVDIIPGLL QGYDNTESSVRKASVFCLVAIYSVIGEDLKPHLAQLTGSKMKLLNLYIKRAQTTNSNSSSSSDVSTHS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_177548 |
| ORF Size | 4404 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_177548.2, NP_808216.2 |
| RefSeq Size | 7623 bp |
| RefSeq ORF | 4407 bp |
| Locus ID | 76707 |
| MW | 162.6 kDa |
| Gene Summary | Microtubule plus-end tracking protein that promotes the stabilization of dynamic microtubules. Involved in the nucleation of noncentrosomal microtubules originating from the trans-Golgi network (TGN). Required for the polarization of the cytoplasmic microtubule arrays in migrating cells towards the leading edge of the cell. May act at the cell cortex to enhance the frequency of rescue of depolymerizing microtubules by attaching their plus-ends to cortical platforms composed of ERC1 and PHLDB2. This cortical microtubule stabilizing activity is regulated at least in part by phosphatidylinositol 3-kinase signaling. Also performs a similar stabilizing function at the kinetochore which is essential for the bipolar alignment of chromosomes on the mitotic spindle (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC224518 | Clasp1 (untagged) - Mouse CLIP associating protein 1 (Clasp1), transcript variant 2, (10ug) |
CNY 18,810.00 |
|
| MG217833 | Clasp1 (tGFP-tagged) - Mouse CLIP associating protein 1 (Clasp1) transcript variant 2, (10ug) |
CNY 16,060.00 |
|
| MR217833L3 | Lenti ORF clone of Clasp1 (Myc-DDK-tagged) - Mouse CLIP associating protein 1 (Clasp1), transcript variant 2 |
CNY 16,530.00 |
|
| MR217833L4 | Lenti ORF clone of Clasp1 (mGFP-tagged) - Mouse CLIP associating protein 1 (Clasp1), transcript variant 2 |
CNY 16,530.00 |
