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Eprs (NM_029735) Mouse Tagged ORF Clone
CAT＃: MR218237
- TrueORF®
Eprs (Myc-DDK-tagged) - Mouse glutamyl-prolyl-tRNA synthetase (Eprs)
ORF Plasmid: tGFP
"NM_029735" in other vectors (4)
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CNY 15,520.00
| Cited in 2 publications. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 2410081F06Rik; 3010002K18Rik; C79379; Qprs |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR218237 representing NM_029735
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCGCTGTGCCTCACGGTGAACGCCGGAAACCCTCCGCTGGAAGCTCTGCTGGCGGTGGAACATG TGAAAGGTGATGTCAGCATTTCTGTGGAAGAAGGGAAAGAGAATCTTCTTCGGGTTTCTGAGACTGTGGC ATTCACTGACGTGAATTCAATCCTGCGCTACCTGGCTAGAATTGCAACTACGTCTGGGCTGTATGGGACT AACCTGATGGAGCACACTGAGATTGATCATTGGTTGGAGTTTAGTGCTACAAAGTTGTCTTCGTGTGATA GGCTGACTTCTGCAATCAACGAGCTTAATCATTGCCTGTCTCTGAGAACATACCTAGTTGGGAACTCCTT GACCTTAGCAGATCTATGTGTTTGGGCCACCCTAAAAGGAAGTGCTGCATGGCAGGAACACTTGAAACAG AACAAAACTCTAGTCCACGTTAAACGCTGGTTTGGCTTTCTTGAGGCCCAGCAGGCCTTCCGCTCAGTTG GCACCAAGTGGGATGTTTCAGGAAACAGAGCTACAGTGGCACCTGACAAAAAACAAGATGTTGGGAAATT CGTGGAGCTTCCAGGTGCAGAGATGGGAAAGGTGACAGTCAGATTCCCCCCAGAGGCTAGTGGTTACTTA CACATTGGGCATGCGAAAGCTGCTCTTCTGAACCAGCACTACCAGGTTAACTTTAAAGGGAAACTGATCA TGAGATTTGATGACACAAACCCAGAAAAGGAAAAGGAAGATTTTGAGAAGGTCATCTTGGAAGATGTTGC AATGCTGCATATTAAACCAGATCAGTTTACTTACACTTCAGATCATTTTGAAACTATAATGAAGTATGCA GAGAAATTAATTCAAGAAGGGAAGGCTTATGTGGATGACACTCCTGCAGAACAGATGAAGGCGGAACGTG AACAGAGGACAGAATCCAAACACAGGAAGAACTCTGTTGAGAAGAATCTACAGATGTGGGAAGAAATGAA AAAAGGAAGCCAGTTTGGTCAGTCCTGTTGCCTGCGAGCAAAAATTGACATGAGTAGTAACAATGGGTGT ATGAGAGACCCGACCCTTTACCGATGCAAAATCCAGCCCCACCCAAGGACTGGGAATAAATACAATGTTT ATCCAACATATGACTTTGCCTGCCCTATAGTGGACAGCATTGAAGGTGTTACTCATGCCTTGAGGACAAC AGAGTACCACGACCGAGATGAACAATTTTATTGGATCATTGAAGCTTTAGGCATAAGGAAACCATACATC TGGGAGTACAGTCGCTTAAATCTCAACAACACCGTGCTGTCCAAAAGAAAGCTCACGTGGTTTGTCAATG AAGGACTGGTAGATGGATGGGATGACCCACGGTTTCCTACAGTTCGTGGAGTTCTGAGAAGAGGGATGAC AGTTGAGGGGCTGAAGCAGTTCATTGCTGCTCAGGGCTCCTCAAGGTCTGTTGTGAACATGGAATGGGAC AAAATCTGGGCGTTCAACAAAAAGGTTATTGACCCAGTGGCTCCACGGTACGTTGCATTGCTGAAGAAGG AAGTCGTCCCAGTGAATGTCCTGGATGCGCAGGAGGAGATGAAGGAAGTAGCCAGACACCCCAAGAATCC TGATGTTGGTTTGAAGCCTGTGTGGTACAGTCCCAAGGTTTTCATAGAAGGGGCCGATGCAGAGACTTTC TCAGAGGGTGAGATGGTCACATTTATAAACTGGGGCAACATCAACATTACTAAAATACACAAAAATGCAG ATGGAAAAATTACATCTCTAGATGCAAAATTGAATTTGGAAAACAAAGACTACAAGAAAACCACCAAGAT CACTTGGCTTGCTGAGAGCACACACGCTCTCTCGATCCCAGCCGTCTGTGTCACTTATGAGCACTTGATC ACAAAGCCAGTGCTGGGAAAAGATGAGGACTTCAAGCAGTACATCAACAAAGACAGTAAGCATGAAGAGC TAATGCTAGGAGATCCTTGCCTTAAAGATCTGAAAAAAGGAGACATCATACAACTGCAGAGGAGAGGGTT CTTCATATGCGACCAGCCATACGAACCTGTCAGCCCATATAGTTGCAGAGAAGCTCCATGCATCCTGATA TACATACCTGATGGGCACACAAAGGAAATGCCAACATCCGGATCAAAGGAAAAGACCAAAGTGGAAATCT CAAAAAAGGAGACAAGCTCTGCTCCTAAAGAAAGACCAGCACCTGCTGTGAGTTCTACTTGTGCTACAGC TGAGGATTCCTCCGTCCTCTACAGCAGAGTGGCGGTGCAGGGGGATGTGGTTCGTGAATTGAAAGCCAAG AAAGCGCCAAAGGAAGATATAGACGCAGCTGTGAAGCAGCTTTTGACTTTGAAAGCTGAATATAAAGAGA AAACTGGGCAGGAGTATAAACCTGGGAACCCCTCTGCTGCAGCAGTACAGACTGTTTCTACAAAGTCATC ATCAAACACTGTGGAAAGTACATCTTTATACAATAAAGTCGCTGCCCAAGGGGAGGTGGTCCGAAAGCTG AAAGCGGAAAAGGCTCCTAAGGCTAAAGTAACTGAAGCTGTGGAATGTTTGCTGTCCCTGAAAGCTGAGT ATAAAGAAAAGACTGGGAAGGACTATGTACCTGGCCAGCCCCCAGCATCGCAGAACTCACATTCAAACCC TGTCAGTAATGCTCAGCCTGCTGGAGCGGAGAAACCAGAAGCCAAAGTGCTATTTGACAGAGTGGCTTGT CAAGGAGAAGTAGTTCGAAAACTTAAAGCTGAAAAGGCCTCTAAGGATCAAGTGGATTCGGCGGTACAAG AACTTCTTCAGCTGAAGGCACAGTACAAGTCGCTGACAGGAATCGAGTATAAGCCTGTGTCTGCTACTGG GGCAGAAGACAAAGATAAAAAGAAGAAAGAAAAAGAAAATAAATCTGAAAAGCAGAACAAGCCTCAGAAA CAAAATGATGGCCAAGGGAAAGACTCTTCCAAAAGCCAAGGCAGTGGCCTGTCATCAGGTGGAGCAGGAG AAGGGCAGGGGCCTAAGAAACAGACCAGATTGGGTCTTGAAGCAAAAAAAGAAGAAAATCTTGCCGAATG GTACTCTCAAGTCATCACAAAGTCCGAGATGATTGAATACTACGATGTGAGTGGCTGCTATATCCTCCGT CCCTGGTCGTACTCCATCTGGGAGTCCATTAAGGACTTCTTTGACGCTGAGATCAAGAAGCTCGGTGTTG AGAACTGCTACTTCCCCATATTTGTGTCTCAGGCTGCGTTAGAGAAAGAGAAGAATCACATCGAAGACTT TGCTCCTGAGGTTGCTTGGGTTACAAGATCAGGAAAAACAGAGTTGGCAGAGCCAATTGCCATTCGTCCT ACTAGTGAAACAGTGATGTATCCGGCATATGCTAAATGGGTGCAGTCCCACAGAGACCTGCCCGTCAGGC TCAACCAGTGGTGCAATGTGGTGCGCTGGGAGTTCAAACACCCACAGCCTTTCCTCCGAACTCGGGAGTT CCTGTGGCAGGAGGGGCACAGCGCCTTTGCCACCTTTGAGGAAGCAGCTGATGAGGTTTTACAGATCCTT GAGTTATATGCTCGAGTATATGAAGAGCTCTTAGCCATTCCTGTTGTGAGAGGAAGGAAGACTGAAAAGG AGAAGTTTGCAGGAGGAGACTACACAACCACAATAGAAGCCTTCATATCTGCCAGCGGACGCGCCATTCA GGGAGCAACATCACACCATTTAGGCCAGAATTTCTCCAAAATGTGTGAAATAGTTTTTGAAGATCCCAAG ACACCAGGAGAAAAGCAGTTTGCATACCAGTGCTCCTGGGGCCTGACAACACGAACCATCGGTGTGATGG TCATGGTCCATGGGGACAACATGGGCCTAGTGCTTCCACCCCGAGTAGCATCTGTCCAGGTGGTAGTCAT TCCCTGTGGAATCACAAATGCCCTGTCTGAAGAAGACAGAGAGGCACTGATGGCAAAATGCAACGAGTAC AGAAGGCGGCTGCTTGGTGCTAACATCCGAGTCCGAGTCGATCTACGAGACAACTACTCCCCAGGCTGGA AGTTCAACCACTGGGAACTCAAGGGAGTTCCAGTCAGACTTGAGGTGGGGCCACGTGATATGAAGAGCTG TCAGTTTGTAGCAGTCAGACGAGATACTGGAGAGAAACTGACAATTGCTGAGAAGGAGGCTGAGGCTAAG CTGGAAAAGGTTTTGGAAGACATCCAGCTGAACCTTTTCACAAGGGCTTCTGAAGACCTTAAAACCCACA TGGTTGTGTCTAATACACTGGAGGACTTCCAGAAGGTGCTAGATGCTGGAAAGGTTGCACAGATTCCATT TTGTGGGGAAATTGACTGTGAGGACTGGATCAAAAAGATGACTGCCAGGGATCAAGATGTGGAACCTGGT GCTCCATCCATGGGAGCCAAAAGCCTTTGCATTCCTTTCAACCCTCTGTGTGAGCTGCAGCCCGGAGCCA TGTGTGTCTGTGGCAAGAATCCTGCCAAGTTCTACACCTTGTTTGGTCGGAGTTAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR218237 representing NM_029735
Red=Cloning site Green=Tags(s) MAALCLTVNAGNPPLEALLAVEHVKGDVSISVEEGKENLLRVSETVAFTDVNSILRYLARIATTSGLYGT NLMEHTEIDHWLEFSATKLSSCDRLTSAINELNHCLSLRTYLVGNSLTLADLCVWATLKGSAAWQEHLKQ NKTLVHVKRWFGFLEAQQAFRSVGTKWDVSGNRATVAPDKKQDVGKFVELPGAEMGKVTVRFPPEASGYL HIGHAKAALLNQHYQVNFKGKLIMRFDDTNPEKEKEDFEKVILEDVAMLHIKPDQFTYTSDHFETIMKYA EKLIQEGKAYVDDTPAEQMKAEREQRTESKHRKNSVEKNLQMWEEMKKGSQFGQSCCLRAKIDMSSNNGC MRDPTLYRCKIQPHPRTGNKYNVYPTYDFACPIVDSIEGVTHALRTTEYHDRDEQFYWIIEALGIRKPYI WEYSRLNLNNTVLSKRKLTWFVNEGLVDGWDDPRFPTVRGVLRRGMTVEGLKQFIAAQGSSRSVVNMEWD KIWAFNKKVIDPVAPRYVALLKKEVVPVNVLDAQEEMKEVARHPKNPDVGLKPVWYSPKVFIEGADAETF SEGEMVTFINWGNINITKIHKNADGKITSLDAKLNLENKDYKKTTKITWLAESTHALSIPAVCVTYEHLI TKPVLGKDEDFKQYINKDSKHEELMLGDPCLKDLKKGDIIQLQRRGFFICDQPYEPVSPYSCREAPCILI YIPDGHTKEMPTSGSKEKTKVEISKKETSSAPKERPAPAVSSTCATAEDSSVLYSRVAVQGDVVRELKAK KAPKEDIDAAVKQLLTLKAEYKEKTGQEYKPGNPSAAAVQTVSTKSSSNTVESTSLYNKVAAQGEVVRKL KAEKAPKAKVTEAVECLLSLKAEYKEKTGKDYVPGQPPASQNSHSNPVSNAQPAGAEKPEAKVLFDRVAC QGEVVRKLKAEKASKDQVDSAVQELLQLKAQYKSLTGIEYKPVSATGAEDKDKKKKEKENKSEKQNKPQK QNDGQGKDSSKSQGSGLSSGGAGEGQGPKKQTRLGLEAKKEENLAEWYSQVITKSEMIEYYDVSGCYILR PWSYSIWESIKDFFDAEIKKLGVENCYFPIFVSQAALEKEKNHIEDFAPEVAWVTRSGKTELAEPIAIRP TSETVMYPAYAKWVQSHRDLPVRLNQWCNVVRWEFKHPQPFLRTREFLWQEGHSAFATFEEAADEVLQIL ELYARVYEELLAIPVVRGRKTEKEKFAGGDYTTTIEAFISASGRAIQGATSHHLGQNFSKMCEIVFEDPK TPGEKQFAYQCSWGLTTRTIGVMVMVHGDNMGLVLPPRVASVQVVVIPCGITNALSEEDREALMAKCNEY RRRLLGANIRVRVDLRDNYSPGWKFNHWELKGVPVRLEVGPRDMKSCQFVAVRRDTGEKLTIAEKEAEAK LEKVLEDIQLNLFTRASEDLKTHMVVSNTLEDFQKVLDAGKVAQIPFCGEIDCEDWIKKMTARDQDVEPG APSMGAKSLCIPFNPLCELQPGAMCVCGKNPAKFYTLFGRSY TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_029735 |
| ORF Size | 4536 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_029735.2 |
| RefSeq Size | 4850 bp |
| RefSeq ORF | 4539 bp |
| Locus ID | 107508 |
| UniProt ID | Q8CGC7 |
| MW | 170.5 kDa |
| Gene Summary | Multifunctional protein which is primarily part of the aminoacyl-tRNA synthetase multienzyme complex, also know as multisynthetase complex, that catalyzes the attachment of the cognate amino acid to the corresponding tRNA in a two-step reaction: the amino acid is first activated by ATP to form a covalent intermediate with AMP and is then transferred to the acceptor end of the cognate tRNA (By similarity). The phosphorylation of EPRS, induced by interferon-gamma, dissociates the protein from the aminoacyl-tRNA synthetase multienzyme complex and recruits it to the GAIT complex that binds to stem loop-containing GAIT elements in the 3' UTR of diverse inflammatory mRNAs (such as ceruplasmin), suppressing their translation. Interferon-gamma can therefore redirect, in specific cells, the EPRS function from protein synthesis to translation inhibition (PubMed:23071094). Also functions as an effector of the mTORC1 signaling pathway by promoting, through SLC27A1, the uptake of long-chain fatty acid by adipocytes. Thereby, it also plays a role in fat metabolism and more indirectly influences lifespan (PubMed:28178239).[UniProtKB/Swiss-Prot Function] |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Hypomyelinating Leukodystrophy 15 (HLD15)-Associated Mutation of EPRS1 Leads to Its Polymeric Aggregation in Rab7-Positive Vesicle Structures, Inhibiting Oligodendroglial Cell Morphological Differentiation
,null,
Polymers
,PubMed ID 33805425
[Eprs]
|
|
Structural control of caspase-generated glutamyl-tRNA synthetase by appended noncatalytic WHEP domains
,Halawani, D;Gogonea, V;DiDonato, JA;Pipich, V;Yao, P;China, A;Topbas, C;Vasu, K;Arif, A;Hazen, SL;Fox, PL;,
J. Biol. Chem.
,PubMed ID 29643180
[EPRS]
|
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC224631 | Eprs (untagged) - Mouse glutamyl-prolyl-tRNA synthetase (Eprs), (10ug) |
CNY 19,380.00 |
|
| MG218237 | Eprs (tGFP-tagged) - Mouse glutamyl-prolyl-tRNA synthetase (Eprs), (10ug) |
CNY 16,630.00 |
|
| MR218237L3 | Lenti ORF clone of Eprs (Myc-DDK-tagged) - Mouse glutamyl-prolyl-tRNA synthetase (Eprs) |
CNY 17,010.00 |
|
| MR218237L4 | Lenti ORF clone of Eprs (mGFP-tagged) - Mouse glutamyl-prolyl-tRNA synthetase (Eprs) |
CNY 17,010.00 |
