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Rab31 (NM_133685) Mouse Tagged ORF Clone
CAT＃: MR218479
- TrueORF®
Rab31 (Myc-DDK-tagged) - Mouse RAB31, member RAS oncogene family (Rab31)
ORF Plasmid: tGFP
"NM_133685" in other vectors (4)
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CNY 2,400.00
CNY 3,705.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 1700093E07Rik; AI415285; Rab22B |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR218479 representing NM_133685
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGGCGATACGGGAGCTCAAAGTGTGTCTTCTCGGGGACACGGGGGTTGGGAAATCCAGCATTGTGT GTCGTTTTGTCCAGGATCACTTTGACCACAACATCAGCCCCACTATTGGGGCATCTTTCATGACCAAAAC CGTGCCTTGTGGAAATGAACTTCACAAATTCCTCATCTGGGACACCGCTGGCCAGGAGCGGTTCCACTCC CTGGCTCCTATGTACTACCGAGGATCTGCTGCAGCCGTCATCGTCTATGACATCACTAAGCAGGATTCAT TTCATACCTTGAAAAAATGGGTCAAGGAGCTGAAGGAGCACGGCCCAGAAAACATTGTGATGGCGATTGC TGGGAACAAGTGTGACCTCTCTGATATTAGGGAGGTGCCCCTGAAGGACGCTAAGGAGTACGCTGAATCC ATAGGTGCCATCGTGGTGGAGACCAGCGCGAAGAATGCCATTAACATCGAGGAGCTCTTTCAAGGAATCA GCCGCCAGATCCCACCCTTGGGCCCTCAGGAAAATGGAAACAGCGGCGGGATCAAGCTTGGGAATCAGAG CTTGCAAGCCAGCCGTCGGTGCTGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR218479 representing NM_133685
Red=Cloning site Green=Tags(s) MMAIRELKVCLLGDTGVGKSSIVCRFVQDHFDHNISPTIGASFMTKTVPCGNELHKFLIWDTAGQERFHS LAPMYYRGSAAAVIVYDITKQDSFHTLKKWVKELKEHGPENIVMAIAGNKCDLSDIREVPLKDAKEYAES IGAIVVETSAKNAINIEELFQGISRQIPPLGPQENGNSGGIKLGNQSLQASRRCC TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_133685 |
| ORF Size | 585 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_133685.2, NP_598446.2 |
| RefSeq Size | 3476 bp |
| RefSeq ORF | 588 bp |
| Locus ID | 106572 |
| UniProt ID | Q921E2 |
| MW | 21.5 kDa |
| Gene Summary | The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. Required for the integrity and for normal function of the Golgi apparatus and the trans-Golgi network. Plays a role in insulin-stimulated translocation of GLUT4 to the cell membrane. Plays a role in the maturation of phagosomes that engulf pathogens, such as S.aureus and Mycobacterium (By similarity). Plays a role in M6PR transport from the trans-Golgi network to endosomes. Plays a role in the internalization of EGFR from the cell membrane into endosomes.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC212128 | Rab31 (untagged) - Mouse RAB31, member RAS oncogene family (Rab31), (10ug) |
CNY 3,990.00 |
|
| MG218479 | Rab31 (tGFP-tagged) - Mouse RAB31 member RAS oncogene family (Rab31), (10ug) |
CNY 2,850.00 |
|
| MR218479L3 | Lenti ORF clone of Rab31 (Myc-DDK-tagged) - Mouse RAB31, member RAS oncogene family (Rab31) |
CNY 4,750.00 |
|
| MR218479L4 | Lenti ORF clone of Rab31 (mGFP-tagged) - Mouse RAB31, member RAS oncogene family (Rab31) |
CNY 4,750.00 |
