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Polr1e (NM_022811) Mouse Tagged ORF Clone
CAT＃: MR219108
- TrueORF®
Polr1e (Myc-DDK-tagged) - Mouse polymerase (RNA) I polypeptide E (Polr1e)
ORF Plasmid: tGFP
"NM_022811" in other vectors (4)
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CNY 4,370.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 53kDa; AU042259; D030019D19Rik; Paf53; Praf1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR219108 representing NM_022811
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTACTCTGGAGTCTCCTGGGATGGATGACCAGGCTGGAGACACGGAGACAGAGGCGCTACAGAGTG CAAGGTGGCTGTACTGTGGAGAGCCCGACGACAGGCAGAAAGCTGTGCTGGTCCAGTTCTCCAATGGGAA GCTGCAGAATCCGGGCGACATGCGCTTCACCTTGTACAACAGCACCGACTTAGTAAACCCCAGGCAGAGG AGTCACCGGATCGTGGCAGCTGAAACAGATAGGCTCTCCTATGTCGGAAACAATTTTGGGACCGGTGCCC TCAAATGCAATGCTCTTTGCAGACACTTTGTGGGAATTCTGAATAAGACCTCTGGCCAGATGGAAGTCTA TGATGCCGAACTGTTCAACATGCAGCCGCTGTTTGCTGACGATGCTATTGAGCGAGAGCCTCCTCTGGAG AATCAGAACAAGACTTTCAGGGACAAGTTGGATTCTTGCATTGAAGCCTTTGGAAGCACCAAGCAGAAGC GGTCCCTAAACTCCAGGCGGATGAACAAAGTTGGCAGCGAGTCTCTGAATCTCAGTGTGGCGAAAGCCGC GGAGAGTATTATTGATACAAAGGGTGTGAATGCGCTGGTCAGCGATGCCATGCAGGATGACTTACAAGAT GGCGTCCTCTACCTTCCTCCCTGCTACGCTGACGCAGCCAAGCCGGAAGACGTGTATAGATTTGAAGACA TTCTGTCCCCGGCCGAGTATGATGCTCTTGAGAGCCCATCCGAAGCCTTCAGAAAGGTCACATCGGAAGA CATACTGAAGATGATTGAAGAGAACAGCCACTGCTCCTACGTCATAGAAATGCTGAAGTCTCTGCCCATA GATGAGGTCCACCGGAACCGGCAAGCCCGGAGCATCTGGTTCCTAGACGCCCTCATCAGATTCCGAGCTC AGAAAGTGATAAAGGGGAAAAGAGCCTTAGGGCCTGGAATCCCGCACATTATCAACACCAAGCTGCTGAA ACAGTTCACCTGCTTGACCTACAACAACGGCAGGTTACAGAACCTCATTTCAAGTTCTATGAGGGCCAAG ATCACTTCGTATGCGATTATACTTGCCCTGCACATAAATAACTTCCAGGTTGACCTGACAGCGTTGCAGA AGGACTTGAAGCTGAGTGAGAAAAGGATGATTGAGATAGCGAAAGCCATGAGGCTGAAGATCTCCAAACA AAAGGTGTCCCTGGCAGATGGCAGGGAAGAGAGTCACAGACTGGGCACTCTGTCTGTCCCACTGCCTCCA GCCCAGAACTCTGATCGACAGTCAAAGCGGAGGAAAATGAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR219108 representing NM_022811
Red=Cloning site Green=Tags(s) MATLESPGMDDQAGDTETEALQSARWLYCGEPDDRQKAVLVQFSNGKLQNPGDMRFTLYNSTDLVNPRQR SHRIVAAETDRLSYVGNNFGTGALKCNALCRHFVGILNKTSGQMEVYDAELFNMQPLFADDAIEREPPLE NQNKTFRDKLDSCIEAFGSTKQKRSLNSRRMNKVGSESLNLSVAKAAESIIDTKGVNALVSDAMQDDLQD GVLYLPPCYADAAKPEDVYRFEDILSPAEYDALESPSEAFRKVTSEDILKMIEENSHCSYVIEMLKSLPI DEVHRNRQARSIWFLDALIRFRAQKVIKGKRALGPGIPHIINTKLLKQFTCLTYNNGRLQNLISSSMRAK ITSYAIILALHINNFQVDLTALQKDLKLSEKRMIEIAKAMRLKISKQKVSLADGREESHRLGTLSVPLPP AQNSDRQSKRRKMN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_022811 |
| ORF Size | 1302 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_022811.3, NP_073722.1 |
| RefSeq Size | 2944 bp |
| RefSeq ORF | 1305 bp |
| Locus ID | 64424 |
| UniProt ID | Q8K202 |
| MW | 49.3 kDa |
| Gene Summary | DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors. Appears to be involved in the formation of the initiation complex at the promoter by mediating the interaction between Pol I and UBTF/UBF.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC215729 | Polr1e (untagged) - Mouse polymerase (RNA) I polypeptide E (Polr1e), (10ug) |
CNY 5,610.00 |
|
| MG219108 | Polr1e (tGFP-tagged) - Mouse polymerase (RNA) I polypeptide E (Polr1e), (10ug) |
CNY 4,850.00 |
|
| MR219108L3 | Lenti ORF clone of Polr1e (Myc-DDK-tagged) - Mouse polymerase (RNA) I polypeptide E (Polr1e) |
CNY 6,270.00 |
|
| MR219108L4 | Lenti ORF clone of Polr1e (mGFP-tagged) - Mouse polymerase (RNA) I polypeptide E (Polr1e) |
CNY 6,270.00 |
