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Lnx1 (NM_001159577) Mouse Tagged ORF Clone
CAT＃: MR219190
- TrueORF®
Lnx1 (Myc-DDK-tagged) - Mouse ligand of numb-protein X 1 (Lnx1), transcript variant 1
ORF Plasmid: tGFP
"NM_001159577" in other vectors (4)
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CNY 5,336.00
CNY 5,800.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Lnx |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR219190 representing NM_001159577
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACCAACCGGACCTTGCAGATGACCCAGATCCCAGTCCCGAGCCTCTGTGCATAGTGTGTGGGCAAA ACCACTCCCCAGAGGAAAACCACTTCTACACGTACACAGAGGATGTAGATGACGACCTCATATGCCACAT CTGTCTGCAAGCATTGCTGGACCCTCTGGACACTCCATGTGGACACACCTACTGCACCCTGTGTCTCACC AACTTCCTGGTGGAGAAGGACTTCTGCCCTGTGGATCGAAAGCCTGTGGTTCTGCAGCACTGCAAGAAAT CCAGTATCTTGGTCAACAAGCTTCTCAATAAGCTGTTGGTCACCTGCCCGTTCACGGAGCACTGCACTGA GGTGCTACAGCGCTGTGATCTCCAGCATCATTTCCAGACAAGCTGCAAAGGGGCCTCCCACTATGGCCTG ACCAAGGACAGGAAAAGGCGCTCTCAAGATGGCTGTCCTGATGGTTGCGCCAGCCTCATGGCAACGACGC TCTCCCCAGAGGTTTCTGCAGCCGCCACCATCTCTTTAATGACAGATGAGCCTGGCCTAGACAACCCTGC CTACGTGTCCTCGGTGGAGGATGGTGAGCCAGTAGCCAACTCCTCAGACTCTGGCCGGAGCAACCGGACT AGGGCACGGCCCTTTGAGCGCTCCACTATGAGAAGCCGGTCTTTCAAGAAGATCAACCGAGCTCTAAGTG CTCTCCGGAGGACGAAGAGCGGGAGTGTCGTTGCCAACCATGTTGACCAGGGCAGGGACAACTCTGAGAA CACCACTGTCCCCGAAGTCTTCCCAAGGTTGTTTCACCTGATTCCAGATGGTGAAATTACAAGCATCAAA ATCAACAGAGCGGATCCCAGCGAAAGCCTCTCCATAAGGCTGGTGGGGGGCAGCGAGACGCCGCTGGTCC ACATCATCATCCAGCATATTTATCGCGATGGAGTGATTGCCAGAGATGGACGGCTGCTGCCAGGAGACAT CATTCTCAAGGTCAACGGCATGGACATCAGCAATGTCCCTCATAACTATGCCGTGCGGCTCCTGCGGCAG CCCTGCCAGGTGCTGCGGCTAACGGTGCTGCGGGAGCAGAAGTTCCGTAGCAGAAGCAATGCGCACGTGC CGGACTCCTATGGACCTCGGGATGACAGCTTCCATGTAATCCTCAACAAAAGCAGCCCCGAGGAGCAGCT TGGCATAAAGCTGGTGCGCCGGGTGGATGAGCCCGGCGTGTTCATCTTCAACGTGCTAAATGGGGGTGTC GCCGACCGACACGGCCAGCTGGAGGAGAATGACCGTGTGCTGGCCATCAACGGGCATGACCTTCGATTTG GCAGTCCAGAGAGTGCAGCTCATCTGATTCAGGCCAGTGAAAGACGCGTCCATCTCGTCGTGTCCCGCCA GGTTCGACAGTCCAGCCCAGACATCTTTCAAGAAGCTGGCTGGATCAGCAATGGCCAGCAATCCCCAGGC CCAGGAGAGAGGAACACAGCTTCTAAGCCTGCAGCCACCTGTCATGAGAAGGTTGTAAGTGTCTGGAAAG ACCCCAGCGAGTCTCTCGGCATGACCGTCGGAGGGGGAGCATCCCACAGGGAATGGGACTTGCCCATCTA TGTCATCAGTGTTGAACCCGGCGGAGTCATAAGCAGAGACGGGAGAATAAAAACAGGTGACATTTTATTG AATGTGAATGGGATTGAGCTCACAGAGGTCAGCCGGACAGAGGCCGTTGCCATACTGAAGAGCGCACCCT CCTCAGTGGTACTCAAAGCCTTGGAAGTCAAGGAACAGGAGGCCCAGGAAGACTGCAGCCCAGCAGCCCT GGACTCCAACCACAACGTGACCCCGCCTGGTGACTGGTCCCCATCCTGGGTCATGTGGCTGGAATTACCA CAGTACTTATGTAACTGTAAAGATGTGATACTGCGAAGGAACACAGCTGGAAGCCTGGGCTTCTGCATTG TGGGAGGTTATGAAGAATACAGTGGGAACAAACCTTTTTTTATCAAGTCCATTGTTGAAGGAACACCTGC ATACAATGACGGAAGAATCAGATGTGGTGACATTCTTCTCGCTGTCAACGGTAGAAGTACATCGGGTATG ATACACGCTTGCCTGGCCAGGATGCTCAAGGAACTTAAAGGGAGAATTACTCTGACCATTGCTTCTTGGC CTGGTACTTTTTTA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >MR219190 representing NM_001159577
Red=Cloning site Green=Tags(s) MNQPDLADDPDPSPEPLCIVCGQNHSPEENHFYTYTEDVDDDLICHICLQALLDPLDTPCGHTYCTLCLT NFLVEKDFCPVDRKPVVLQHCKKSSILVNKLLNKLLVTCPFTEHCTEVLQRCDLQHHFQTSCKGASHYGL TKDRKRRSQDGCPDGCASLMATTLSPEVSAAATISLMTDEPGLDNPAYVSSVEDGEPVANSSDSGRSNRT RARPFERSTMRSRSFKKINRALSALRRTKSGSVVANHVDQGRDNSENTTVPEVFPRLFHLIPDGEITSIK INRADPSESLSIRLVGGSETPLVHIIIQHIYRDGVIARDGRLLPGDIILKVNGMDISNVPHNYAVRLLRQ PCQVLRLTVLREQKFRSRSNAHVPDSYGPRDDSFHVILNKSSPEEQLGIKLVRRVDEPGVFIFNVLNGGV ADRHGQLEENDRVLAINGHDLRFGSPESAAHLIQASERRVHLVVSRQVRQSSPDIFQEAGWISNGQQSPG PGERNTASKPAATCHEKVVSVWKDPSESLGMTVGGGASHREWDLPIYVISVEPGGVISRDGRIKTGDILL NVNGIELTEVSRTEAVAILKSAPSSVVLKALEVKEQEAQEDCSPAALDSNHNVTPPGDWSPSWVMWLELP QYLCNCKDVILRRNTAGSLGFCIVGGYEEYSGNKPFFIKSIVEGTPAYNDGRIRCGDILLAVNGRSTSGM IHACLARMLKELKGRITLTIASWPGTFL SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001159577 |
| ORF Size | 2184 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001159577.1, NP_001153049.1 |
| RefSeq Size | 2571 bp |
| RefSeq ORF | 2187 bp |
| Locus ID | 16924 |
| UniProt ID | O70263 |
| MW | 80.2 kDa |
| Gene Summary | E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of NUMB. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Mediates ubiquitination of isoform p66 and isoform p72 of NUMB, but not that of isoform p71 or isoform p65.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC221080 | Lnx1 (untagged) - Mouse ligand of numb-protein X 1 (Lnx1), transcript variant 1, (10ug) |
CNY 9,310.00 |
|
| MG219190 | Lnx1 (tGFP-tagged) - Mouse ligand of numb-protein X 1 (Lnx1) transcript variant 1, (10ug) |
CNY 6,370.00 |
|
| MR219190L3 | Lenti ORF clone of Lnx1 (Myc-DDK-tagged) - Mouse ligand of numb-protein X 1 (Lnx1), transcript variant 1 |
CNY 7,700.00 |
|
| MR219190L4 | Lenti ORF clone of Lnx1 (mGFP-tagged) - Mouse ligand of numb-protein X 1 (Lnx1), transcript variant 1 |
CNY 7,700.00 |
