Plxnb3 (NM_019587) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	AI451018; Plxn6
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR219689 representing NM_019587 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGACTGACTTCCTCCAGGCTCCTGTGATGGCTCCCTGGTCTCCCTTCAGCCTCCACCTGCTGCTGC TGTTCCTGCCGCTGCTGCCTTTGACCAGGGTCCATCGCTTCTCTGTACCAAATACCAGCTTCAACCACCT GGTGCTGGCACCAGATCAGGGCAAACTCTACGTGGGTGCAGTGAATCACCTCTTTCAGCTCAGCCCTGAG CTGAAGATGGAGTCTGTGGCTGTCACTGGCCCTGTCATTGACAGTCCTGATTGTGTGCCTTTCCGTGACC TGGCTGAGTGCCCACAGGCCCAGCTCACTGACAATGCCAACCAGCTCTTGTTGGTGAGCAGCAGGACACA GGAACTGGTGGCTTGTGGGCAAGTGAAGCAGGGTGTATGTGAGAAGCGGCGCCTTGGGGATGTGACTCAA GTGTTGTACCAGGCTGAGGACCCTGGTGATGGGCAGTTTGTGGCTGCCAATACCCTGGGAGTGACCACAG TAGGCCTAGTGGTGCCCTTGCCAGGCCGAGACCTCCTGCTAGTGGCCAGAGGCCTGGCAGGCAAGCTGTC AGCAGGAGTGCCACCCCTAACTGTGCGCCAGCTGGCTGGGCCACAGCCTTTCTCCAGTGAAGGACTGGGA AGACTTGTGGTCGGTGACTTTTCAGACTACAACAATAGCTATGTAGGAGCTTTTTCTGATGCTCACTCTG CCTACTTTGTGTTCCGTCGCCGTGGGGCTCGGGCCCAGACTGAGTATCGCTCCTATGTAGCTCGTGTCTG CCTCAGAGATGTCAACCTTTATTCCTATGTGGAAATGCCACTTACCTGCCATGGCCAGGGCCTTATCCAG GCTGCCTTCCTTACTCCAGACACTTTGCTAGGTGCATTTTCAGCAGGCACAAGTCAGGCACAGGCGGCCC TGTGTGCCTTTCCCTTAGCTGACCTGGACAGGAGTATGGAACAAGCCAGGCGACTTTGCTATACTACTGG TGGTCAGGGTCCTAGCGGCATGGAAGAAGCCACTGTGGAGTATGGTGTCACATCCCGTTGCGTCACTTTG CCTCCTGACTCCCCCGAGTCATACCCCTGTGGTGATGAACACACCCCCAGCCCCATCGCAGGCCGCCAAC CCCTGGAGGCCCAGCCTCTGCTGCAACTGGGACAGTCCATCAGTGCAGTGGCTGCTCTCCAGACAGATGG GCACACGATAGCCTTCCTGGGAGACACCCAAGGCCAGCTACATAAGGTCTTTCTAAACAGCTCCCATGGC CAGGTATACCATTCCCAGCAAGTGGGGCCCCCAGGCTCAGCCATCAGTCCAGATCTGCTGGTAGACAGCA ATGGAGACCACCTCTATGTCTTGACTGCCCAGCAGGTAGACCGGATACTTGTGGCAGCCTGTCCTCAGTT TCCTAACTGCACTACCTGTCTCCAGGCCAGGGACCCACTATGTGGTTGGTGCATCCTTCAGGGAAGGTGT ACTCGGAGAGGCGAGTGTGGACGGGCAGCCCAGCCAAACCACTGGCTGTGGAGCTACGAAGACAACCACT GCCCTTACATCCAGAGCCTGCTACCAGCCCAACACCCCCGTCAGGAACAGGGTCAGATCATTCTGTCTGT TCCTCGGCTGCCCACCCTGGCCATGGATGAATACTTCCACTGTGCTTTTGGGGGCTACAACAGTCTAGCT CAGGTGGAAGAACCCCATGTGGTCTGTACCACCCCTCCTCAAGATCAGATGCCACCTAATCCTCCAGGCT CAGATCATGTCACCTTGCCCCTGGCCCTAATGTTTGAGGATGTGGTATTGACCGCCACCACATTCTCCTT CTATGACTGCAGTGCTGTTCAGGCCTTGGAAGTGGCTGCCCCGTGCCGCGCTTGTGTGAGCAGCCTGTGG CGGTGCCACTGGTGCCCCCAGAGTAGCCACTGTATATATGGAGAGCACTGCCCAGAGGGTGAAAAGGCTG TCTACAGTGCCCAGGAGGTGGACATCCTGGTTCGTGGCCCAGAGGCTTGCCCCCAGGTTGAGGGTCTAGC AAGTCCCCAGTTGGTGCCTGTGGGTTGGGAGAGCCATGTGACCCTGCACATTCAGAACCTTCACTATTTC CAAGGTCTGCCTGCCTTGTACCACTGCTGGCTAGAGCTGCCTGGAAAACTGCAAAAGCTACCTGCCTCCT TAGAGGAGACATCCAGGGACTCTGGCCTCATTCACTGCCAGGCCCAGCAGTTCTACCCCTCTATGTCTCA GTGGGAGCTCCCAGTGCCCATCTATGTAACCAGGGGTGAGATCCAGCGGCTGGACAATGCTGGTGATCTT CATGTGACTCTATATGACTGTGCCATGGGTCACCCTGACTGCAGCCACTGCCAGGCGGCCAATGGGAGCC TGAGCTGTTTGTGGTGTGGTGATGGCCAGCCTGCCTGCCGCTATGGGCCTTTGTGCCCACCAGGTGCTGT GGAACAGCTATGCCCTATACCCAGCATTGATGTGATTGAACCCCTGACTGGTCCCCCAGAAGGTGGCTTG GCCATCACCATCCTGGGTTCCAACCTGGGCCAGGCCTTTAATGATGTGCGAAATGCTGTAACTGTGGCTG GCCAGCCCTGCAACCCTGACCCTTCTCTCTACCGCATCTCTGCTCGGATTGTATGTGTGACATCTCCTGC TCCCAATGGCACTGCTGGGCCAGTCCAAGTGGCCATTAAGAGTCGTCCTCCTGGCATCTCAACCCAGAAC TTCACCTACCAGGACCCTGTCCTGCTGAGCCTGAATCCTCAGTGGGGCCCCCAGGCAGGGGGCACCCAGC TCACCATCCATGGGCAGTATCTCCAGACAGGAGGCAACATCAGTGTCTTTGTGGGTGACCAACCTTGCCC TATCCAGGAGCCTGTGTGTCCTGAAGCCATCATATGCCACACCATGCCCCAGACTGAGCCAGGAGAAGCA GTGGTCCTCATAGTCTTTGGGCATGTTGAACGCAAACTGCTCACCACCCCTTTTCGATACACAGCCAATC CCCAACTGGTGGAAGCAGAGCCTAGTGTCAGCTTCCGGGGAGGTGGACGAGTGATTCGAGTCAGGGGTAC AGGCTTAGACGTTGTGTGGCAGCCCTTGCTGTCTGTGTGGCTGGAGGATGAGCCCAAAGTAAAGGCCTTG GGGGTTCAGGCCCAGGATGCGAACCCAAGGAGGAGCTGTGGTGCCCCTGCTGCAGATCCTCAGGCCTGTA TCCATCTTGAGAGTGGCTTACTACAGTGTTCCACGCTCTGCTCTGTCAACTCATCCAGCCTCCTCCTGTG CCACAGTCCAGCAGTGCCAGATGGGGCCCTCCCAAAGCGAGTCTTCTTTGCCCTAGACAACATGCAAGTA GACTTTGCCAGTGCTAGTGGGGGCCAGGGATTCCTATACCAGCCTAACCCTCGCCTGGCTCCACTTAGTC ATGAGGGGATTACTCACCCCTACCACCTCAAGCCAGGCCATGTCCTAGATGTGGAGGGCGAGGGCCTCAA TCTGGGGATCAGCAAGGAGGAGGTGCAGGTGCATATTGGAGATGGAGAGTGCCTGGTGAAGACACTGACG CTTACTCACTTGTACTGTGAGCCACCACCCCAAGCCCCTCAACCCACCAATGGTTCAGGCACCCTGCCGC AGTTTGTGGTGCAGATGGGCAACCTGCGCCTGGCTCTAGGCCCTGTCCAGTACGAAGCAGAGTCCATGAT GTCCACCTTTCCTGTGGAGGCCCAGTTGGGCCTGGGCATGGGTGCTGCCGTGCTGATTGCTGCGGTGCTC CTCCTCACCCTCATGTACAGGCACAAGAGCAAGAAGGCCTTGCGAGACTATCAGAAGGTGCTGGTGCAGT TGGAGAACCTGGAGACTGGTGTGGGTGACCAGTGCCGCAAGGAGTTCACAGACTTGATGACTGAGATGAC AGACCTCACCAGTGACCTAGAGGCCAGTGGGATTCCCTTCCTGGACTACCGAACATATGCTGAGCGTGCC TTCTTCCCCGGCCATGTTGGCTGCCCACTGCAGCCAGGGCTCGAGGGGCTTGGGGAAGAGGGTCGCAGTG TCACCGTGCGCCAGGGTCTCACACAACTCTCCAATTTGCTCAACAGCAAGCTCTTCCTTCTCACACTCAT CCACACCTTAGAGGAGCAGCCAAGCTTCTCCCAGCGGGACCGCTGCCATGTGGCTTCACTACTTTCCCTG GCTCTGCACAGCAAGCTTGAGTATTTGACCGACATCATGAGAACTCTGCTTGGTGACCTAGCGGCCCATT ATGTACACAAGAACCCCAAGCTCATGTTACGCAGGACGGAGACCATGGTAGAGAAGCTGCTCACCAACTG GCTATCTATCTGTCTCTACACCTTCTTGAAGGAAGTGGCTGGCGAGCCACTGTACATGCTTTTCCGAGCC ATCAAGTACCAGGTGGACAAGGGCCCTGTGGACGCTGTGACAGGCAAGGCAAAACGAACTCTGAATGACA GCCACCTGCTACGAGAGGATGTGGAGTTCCAGCCCCTGACCCTGATGGCACTGGTGGGACCAGAGGCTGA TAGGGCAGCAGGGAACAGTGGAGTGCATCGAGTGCCAGCCAGGGTGCTTGATACAGACACCATCACCCAA GTCAAGGAGAAGGTGTTGGACCAAATCTACAAGGGAACCCCCTTCTCCCAGAGGCCTTCAGTGCACTCCC TAGATCTTGAGTGGCGCTCAGGCCTAGCTGGTCACCTAACCCTGTCAGATGAGGACTTGACCTCTGTGAC TCAAAACCACTGGAAGAGACTCAACACCCTACAACACTATAAGGTCCCAGATGGAGCAACAGTGGTACTC ATTCCTCAGGTGCACAATGGTGGCACTGTTTCCCAGAGTCTGGGCCAGACTGGCTGCCCTTCTGGAGAGA ACACTCCCATGCTGGAAGATGGTGAGGAAGGTGGAGTTCGGCTCTGGCACCTGGTAAAGGCCACTGAGGA GGCAGAAGGGGCCAAAGTGCGGCGCAGCAGCCTCCGAGATCGAGAACGAGAACGGTCACGAGCCAAGGCC ATTCCTGAAATCTATCTTACTCGCTTACTTTCAATGAAGGGCACCCTGCAGAAGTTTGTGGATGACACCT TCCAGGCCATTCTTAGCATGAATCGGCCTGTGCCTATTGCTGTTAAGTACCTATTTGACTTCCTGGATGA GCTGGCAGAGAAACATGGCATTGAAGATCCAGAGACCCTGCACATTTGGAAGACAAACAGCCTACTTCTG CGATTCTGGGTGAATGTCTTGAAGAACCCACAGCTCATCTTTGATGTACAAGTGTCAGACAACGAGGATG CCATCTTGGCTGTCATTGCACAGACTTTCATCGATTCCTGCATGGTCTCGGAGCATAAAGTGGGCCGGGA TTCCCCAGTGAACAAACTGCTCTATGCCCGGGAGATCCCTCGCTACAAGCAGATGGTGGAGAAGTACTAT GCGGACATTCGCCAGAGCTCTCCAGCAAGCTATCAAGAGATGAACTCAGCTCTGGCTGAACTCTCTGGGA ATTATTCCTCTGCTCCCCACTGCCTGGAGGCTCTTCGAGAACTTTACAACCACATCCACAGGTATTATGA CCAGATTATCAGTGCCCTGGAGGAGGACCCTGTGGCCCAGAAGATGCAGCTGGCTTGCCGCCTGCAGCAG GTGGCTGCCTTGGTGGAATACAAAGTGACTGACCTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR219689 representing NM_019587 Red=Cloning site Green=Tags(s) MLTDFLQAPVMAPWSPFSLHLLLLFLPLLPLTRVHRFSVPNTSFNHLVLAPDQGKLYVGAVNHLFQLSPE LKMESVAVTGPVIDSPDCVPFRDLAECPQAQLTDNANQLLLVSSRTQELVACGQVKQGVCEKRRLGDVTQ VLYQAEDPGDGQFVAANTLGVTTVGLVVPLPGRDLLLVARGLAGKLSAGVPPLTVRQLAGPQPFSSEGLG RLVVGDFSDYNNSYVGAFSDAHSAYFVFRRRGARAQTEYRSYVARVCLRDVNLYSYVEMPLTCHGQGLIQ AAFLTPDTLLGAFSAGTSQAQAALCAFPLADLDRSMEQARRLCYTTGGQGPSGMEEATVEYGVTSRCVTL PPDSPESYPCGDEHTPSPIAGRQPLEAQPLLQLGQSISAVAALQTDGHTIAFLGDTQGQLHKVFLNSSHG QVYHSQQVGPPGSAISPDLLVDSNGDHLYVLTAQQVDRILVAACPQFPNCTTCLQARDPLCGWCILQGRC TRRGECGRAAQPNHWLWSYEDNHCPYIQSLLPAQHPRQEQGQIILSVPRLPTLAMDEYFHCAFGGYNSLA QVEEPHVVCTTPPQDQMPPNPPGSDHVTLPLALMFEDVVLTATTFSFYDCSAVQALEVAAPCRACVSSLW RCHWCPQSSHCIYGEHCPEGEKAVYSAQEVDILVRGPEACPQVEGLASPQLVPVGWESHVTLHIQNLHYF QGLPALYHCWLELPGKLQKLPASLEETSRDSGLIHCQAQQFYPSMSQWELPVPIYVTRGEIQRLDNAGDL HVTLYDCAMGHPDCSHCQAANGSLSCLWCGDGQPACRYGPLCPPGAVEQLCPIPSIDVIEPLTGPPEGGL AITILGSNLGQAFNDVRNAVTVAGQPCNPDPSLYRISARIVCVTSPAPNGTAGPVQVAIKSRPPGISTQN FTYQDPVLLSLNPQWGPQAGGTQLTIHGQYLQTGGNISVFVGDQPCPIQEPVCPEAIICHTMPQTEPGEA VVLIVFGHVERKLLTTPFRYTANPQLVEAEPSVSFRGGGRVIRVRGTGLDVVWQPLLSVWLEDEPKVKAL GVQAQDANPRRSCGAPAADPQACIHLESGLLQCSTLCSVNSSSLLLCHSPAVPDGALPKRVFFALDNMQV DFASASGGQGFLYQPNPRLAPLSHEGITHPYHLKPGHVLDVEGEGLNLGISKEEVQVHIGDGECLVKTLT LTHLYCEPPPQAPQPTNGSGTLPQFVVQMGNLRLALGPVQYEAESMMSTFPVEAQLGLGMGAAVLIAAVL LLTLMYRHKSKKALRDYQKVLVQLENLETGVGDQCRKEFTDLMTEMTDLTSDLEASGIPFLDYRTYAERA FFPGHVGCPLQPGLEGLGEEGRSVTVRQGLTQLSNLLNSKLFLLTLIHTLEEQPSFSQRDRCHVASLLSL ALHSKLEYLTDIMRTLLGDLAAHYVHKNPKLMLRRTETMVEKLLTNWLSICLYTFLKEVAGEPLYMLFRA IKYQVDKGPVDAVTGKAKRTLNDSHLLREDVEFQPLTLMALVGPEADRAAGNSGVHRVPARVLDTDTITQ VKEKVLDQIYKGTPFSQRPSVHSLDLEWRSGLAGHLTLSDEDLTSVTQNHWKRLNTLQHYKVPDGATVVL IPQVHNGGTVSQSLGQTGCPSGENTPMLEDGEEGGVRLWHLVKATEEAEGAKVRRSSLRDRERERSRAKA IPEIYLTRLLSMKGTLQKFVDDTFQAILSMNRPVPIAVKYLFDFLDELAEKHGIEDPETLHIWKTNSLLL RFWVNVLKNPQLIFDVQVSDNEDAILAVIAQTFIDSCMVSEHKVGRDSPVNKLLYAREIPRYKQMVEKYY ADIRQSSPASYQEMNSALAELSGNYSSAPHCLEALRELYNHIHRYYDQIISALEEDPVAQKMQLACRLQQ VAALVEYKVTDL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_019587
ORF Size	5706 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_019587.2, NP_062533.2
RefSeq Size	6024 bp
RefSeq ORF	5709 bp
Locus ID	140571
UniProt ID	Q9QY40
MW	208.4 kDa
Gene Summary	Receptor for SEMA5A that plays a role in axon guidance, invasive growth and cell migration. Stimulates neurite outgrowth and mediates Ca(2+)/Mg(2+)-dependent cell aggregation. In glioma cells, SEMA5A stimulation of PLXNB3 results in the disassembly of F-actin stress fibers, disruption of focal adhesions and cellular collapse as well as inhibition of cell migration and invasion through ARHGDIA-mediated inactivation of RAC1 (By similarity). Seem to be non-essential for normal development and function of the central nervous system.[UniProtKB/Swiss-Prot Function]

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