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Smc1a (NM_019710) Mouse Tagged ORF Clone
CAT＃: MR221566
- TrueORF®
Smc1a (Myc-DDK-tagged) - Mouse structural maintenance of chromosomes 1A (Smc1a)
ORF Plasmid: tGFP
"NM_019710" in other vectors (4)
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CNY 12,260.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 5830426I24Rik; mKIAA0178; Sb1.8; SMC-1A; Smc1; Smc1alpha; Smc1l1; Smcb |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR221566 representing NM_019710
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGTTTCCTGAAACTGATTGAGATCGAGAACTTTAAATCGTACAAGGGTCGACAGATTATCGGACCAT TTCAGAGGTTTACCGCCATCATTGGACCCAATGGCTCTGGTAAGTCAAATCTCATGGATGCCATCAGCTT TGTGCTTGGTGAAAAGACCAGCAACCTGAGGGTAAAGACCCTGCGGGACCTGATACATGGAGCACCTGTG GGCAAGCCGGCTGCCAACCGAGCCTTTGTCAGCATGGTCTACTCGGAGGAGGGTGCTGAGGACCGTACCT TTGCCCGAGTCATTGTAGGGGGCTCTTCTGAGTACAAGATCAACAACAAAGTTGTCCAGCTACATGAATA CAGTGAAGAGTTAGAGAAGTTGGGCATTCTCATCAAAGCTCGTAACTTCCTCGTCTTCCAGGGTGCAGTG GAGTCTATTGCCATGAAGAACCCCAAGGAGAGGACAGCTCTGTTTGAAGAAATTAGTCGCTCTGGGGAAC TTGCACAGGAGTATGACAAGCGAAAGAAAGAAATGGTGAAGGCTGAAGAAGACACACAGTTTAATTATCA TCGCAAGAAAAATATTGCAGCTGAACGAAAGGAGGCCAAACAAGAAAAGGAAGAGGCGGACCGCTATCAG CGCCTGAAGGATGAGGTAGTACGAGCACAGGTACAGTTGCAGCTCTTTAAACTCTACCACAACGAAGTGG AGATTGAGAAGCTCAACAAGGAACTGGCCTCGAAGAACAAGGAAATTGAGAAGGACAAGAAGCGGATGGA CAAGGTAGAGGATGAGCTGAAGGAGAAGAAGAAAGAGCTGGGAAAAATGATGCGGGAACAGCAGCAGATT GAGAAGGAGATCAAGGAAAAGGACTCGGAATTGAATCAGAAGAGGCCTCAGTACATCAAAGCAAAAGAGA ATACATCCCACAAAATCAAGAAGCTCGAAGCAGCCAAGAAGTCTCTTCAAAATGCTCAGAAACATTATAA GAAACGTAAAGGTGACATGGATGAGCTGGAGAAGGAGATGCTGTCAGTGGAGAAGGCCCGACAGGAGTTT GAAGAACGGATGGAGGAAGAGAGTCAGAGTCAGGGCAGAGACTTGACCCTGGAGGAGAACCAGGTAAAGA AATACCACCGATTGAAAGAAGAAGCCAGCAAGAGAGCAGCTACTTTGGCTCAGGAGCTGGAGAAGTTCAA TCGAGACCAGAAAGCTGACCAGGACCGTCTAGATCTGGAAGAGCGGAAGAAAGTAGAAACAGAGGCCAAG ATCAAGCAAAAACTGCGGGAGATTGAAGAGAATCAGAAACGGATTGAGAAGCTAGAAGAATATATTACCA CTAGCAAGCAGTCTCTAGAGGAGCAGAAGAAGCTAGAGGGAGAACTGACGGAAGAAGTAGAGATGGCCAA GCGTCGTATTGATGAGATCAATAAGGAGCTGAACCAGGTGATGGAGCAATTAGGGGATGCACGAATTGAC CGACAGGAGAGTAGCCGCCAGCAGCGAAAGGCAGAAATAATGGAAAGCATCAAGCGTCTCTACCCTGGCT CTGTGTATGGCCGCCTCATTGACCTCTGCCAGCCTACACAAAAGAAGTATCAGATTGCTGTGACCAAGGT TTTAGGGAAGAACATGGATGCTATTATTGTAGACTCAGAGAAGACAGGTCGGGACTGTATTCAGTATATC AAGGAGCAACGTGGAGAGCCTGAGACCTTCTTGCCTCTTGACTACCTGGAGGTGAAACCTACTGATGAGA AACTCCGGGAGCTGAAGGGGGCCAAGCTAGTGATTGATGTAATTCGTTATGAACCACCTCACATTAAAAA AGCTCTGCAGTATGCTTGTGGCAATGCCCTTGTTTGTGATAATGTGGAGGATGCTCGACGCATTGCCTTT GGAGGTCACCAACGCCACAAGACAGTGGCGTTGGATGGCACCCTGTTCCAGAAGTCAGGAGTTATCTCTG GTGGAGCCAGTGATCTCAAGGCAAAAGCACGGCGCTGGGATGAGAAAGCAGTAGACAAACTGAAAGAAAA AAAAGAACGTTTGACAGAGGAGCTGAAAGAGCAGATGAAGGCAAAACGGAAAGAGGCAGAACTGCGTCAA GTGCAGTCTCAGGCCCACGGGCTTCAAATGCGGCTGAAGTACTCACAGAGTGACCTAGAACAGACTAAGA CAAGGCATCTTGCCCTGAATCTCCAGGAAAAGTCTAAGCTGGAGAGTGAACTAGCCAACTTCGGGCCTCG CATTAATGATATCAAGAGAATCATACAGAGCCGAGAGAGGGAAATGAAAGACTTGAAGGAGAAGATGAAC CAGGTGGAAGATGAGGTGTTTGAAGAGTTTTGTCGGGAAATTGGTGTGCGTAACATCCGAGAATTTGAGG AAGAAAAGGTGAAGCGGCAGAATGAAATTGCCAAGAAACGCTTGGAATTTGAGAATCAGAAGACTCGCTT GGGCATCCAATTGGATTTTGAAAAGAATCAGTTGAAGGAGGACCAGGATAAAGTACACATGTGGGAACAG ACGGTGAAAAAGGATGAAAATGAGATAGAAAAGCTTAAGAAGGAGGAACAAAGACACATGAAGATAATAG ATGAAACCATGGCTCAGCTTCAAGACCTTAAGAATCAGCACCTGGCCAAAAAGTCAGAAGTAAACGACAA GAATCATGAGATGGAAGAGATTCGCAAGAAGCTTGGGGGTGCCAACAAGGAAATGACTCATTTACAGAAG GAAGTGACAGCCATTGAGACCAAACTTGAACAGAAGCGCAGTGACCGCCACAACCTGCTACAGGCCTGTA AGATGCAGGACATCAAACTGCCACTGTCAAAGGGCACCATGGATGATATTAGTCAGGAAGAAGGTAGCTC CCAGGGAGAAGAGTCAGTGAGTGGTTCCCAGAGAACATCCAGTATCTATGCTCGAGAGGCCCTCATTGAG ATTGACTACGGTGACCTGTGTGAAGATCTGAAGGATGCACAGGCGGAGGAAGAAATCAAGCAAGAAATGA ACACACTCCAGCAAAAGCTGAATGAGCAGCAGAGTGTGCTTCAGCGGATTGCTGCCCCTAACATGAAGGC CATGGAAAAGCTGGAGAGTGTCCGAGACAAGTTCCAGGAGACCTCAGATGAGTTTGAAGCAGCACGAAAG CGAGCAAAGAAGGCCAAGCAGGCATTTGAACAGATAAAGAAGGAGCGCTTCGACCGATTCAATGCTTGCT TTGAATCTGTGGCTACCAACATTGATGAGATCTATAAGGCTCTGTCCCGTAACAGCAGTGCCCAGGCATT CTTGGGCCCTGAGAATCCTGAAGAGCCCTATTTGGATGGCATCAACTACAACTGTGTAGCTCCTGGCAAA CGTTTCCGGCCTATGGACAATTTGTCAGGCGGGGAGAAGACAGTTGCAGCTCTGGCCTTGCTTTTTGCCA TCCATAGCTACAAGCCAGCCCCCTTCTTCGTCCTGGATGAGATTGACGCTGCCTTGGATAACACCAACAT TGGCAAGGTGGCAAATTACATCAAGGAACAGTCAACTTGCAACTTCCAGGCCATTGTTATCTCTCTCAAG GAGGAATTCTACACCAAGGCTGAGAGCCTCATTGGAGTCTATCCTGAGCAAGGGGACTGTGTGATCAGCA AAGTCCTGACTTTCGACCTCACCAAGTACCCAGATGCCAACCCCAACCCCAATGAACAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR221566 representing NM_019710
Red=Cloning site Green=Tags(s) MGFLKLIEIENFKSYKGRQIIGPFQRFTAIIGPNGSGKSNLMDAISFVLGEKTSNLRVKTLRDLIHGAPV GKPAANRAFVSMVYSEEGAEDRTFARVIVGGSSEYKINNKVVQLHEYSEELEKLGILIKARNFLVFQGAV ESIAMKNPKERTALFEEISRSGELAQEYDKRKKEMVKAEEDTQFNYHRKKNIAAERKEAKQEKEEADRYQ RLKDEVVRAQVQLQLFKLYHNEVEIEKLNKELASKNKEIEKDKKRMDKVEDELKEKKKELGKMMREQQQI EKEIKEKDSELNQKRPQYIKAKENTSHKIKKLEAAKKSLQNAQKHYKKRKGDMDELEKEMLSVEKARQEF EERMEEESQSQGRDLTLEENQVKKYHRLKEEASKRAATLAQELEKFNRDQKADQDRLDLEERKKVETEAK IKQKLREIEENQKRIEKLEEYITTSKQSLEEQKKLEGELTEEVEMAKRRIDEINKELNQVMEQLGDARID RQESSRQQRKAEIMESIKRLYPGSVYGRLIDLCQPTQKKYQIAVTKVLGKNMDAIIVDSEKTGRDCIQYI KEQRGEPETFLPLDYLEVKPTDEKLRELKGAKLVIDVIRYEPPHIKKALQYACGNALVCDNVEDARRIAF GGHQRHKTVALDGTLFQKSGVISGGASDLKAKARRWDEKAVDKLKEKKERLTEELKEQMKAKRKEAELRQ VQSQAHGLQMRLKYSQSDLEQTKTRHLALNLQEKSKLESELANFGPRINDIKRIIQSREREMKDLKEKMN QVEDEVFEEFCREIGVRNIREFEEEKVKRQNEIAKKRLEFENQKTRLGIQLDFEKNQLKEDQDKVHMWEQ TVKKDENEIEKLKKEEQRHMKIIDETMAQLQDLKNQHLAKKSEVNDKNHEMEEIRKKLGGANKEMTHLQK EVTAIETKLEQKRSDRHNLLQACKMQDIKLPLSKGTMDDISQEEGSSQGEESVSGSQRTSSIYAREALIE IDYGDLCEDLKDAQAEEEIKQEMNTLQQKLNEQQSVLQRIAAPNMKAMEKLESVRDKFQETSDEFEAARK RAKKAKQAFEQIKKERFDRFNACFESVATNIDEIYKALSRNSSAQAFLGPENPEEPYLDGINYNCVAPGK RFRPMDNLSGGEKTVAALALLFAIHSYKPAPFFVLDEIDAALDNTNIGKVANYIKEQSTCNFQAIVISLK EEFYTKAESLIGVYPEQGDCVISKVLTFDLTKYPDANPNPNEQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_019710 |
| ORF Size | 3699 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_019710.2, NP_062684.2 |
| RefSeq Size | 3980 bp |
| RefSeq ORF | 3702 bp |
| Locus ID | 24061 |
| UniProt ID | Q9CU62 |
| MW | 143.2 kDa |
| Gene Summary | Involved in chromosome cohesion during cell cycle and in DNA repair. Involved in DNA repair via its interaction with BRCA1 and its related phosphorylation by ATM, and works as a downstream effector in the ATM/NBS1 branch of S-phase checkpoint (By similarity). Central component of cohesin complex. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Involved in DNA repair via its interaction with BRCA1 and its related phosphorylation by ATM, or via its phosphorylation by ATR. Works as a downstream effector both in the ATM/NBS1 branch and in the ATR/MSH2 branch of S-phase checkpoint.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC224011 | Smc1a (untagged) - Mouse structural maintenance of chromosomes 1A (Smc1a), (10ug) |
CNY 15,770.00 |
|
| MG221566 | Smc1a (tGFP-tagged) - Mouse structural maintenance of chromosomes 1A (Smc1a), (10ug) |
CNY 13,490.00 |
|
| MR221566L3 | Lenti ORF clone of Smc1a (Myc-DDK-tagged) - Mouse structural maintenance of chromosomes 1A (Smc1a) |
CNY 14,160.00 |
|
| MR221566L4 | Lenti ORF clone of Smc1a (mGFP-tagged) - Mouse structural maintenance of chromosomes 1A (Smc1a) |
CNY 14,160.00 |
