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Klrk1 (NM_001083322) Mouse Tagged ORF Clone
CAT＃: MR222529
- TrueORF®
Klrk1 (Myc-DDK-tagged) - Mouse killer cell lectin-like receptor subfamily K, member 1 (Klrk1), transcript variant 2
ORF Plasmid: tGFP
"NM_001083322" in other vectors (3)
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CNY 2,190.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | D6H12S2489E; NKG2-D; Nkg2d |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR222529 representing NM_001083322
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCAAATGCCATAATTACGACCTCAAGCCAGCAAAGTGGGATACTTCTCAAGAACAACAGAAACAAA GATTAGCACTAACTACCAGTCAACCTGGAGAAAATGGTATCATAAGAGGAAGATACCCTATAGAAAAACT CAAAATATCTCCAATGTTCGTTGTTCGAGTCCTTGCTATAGCCTTGGCAATTCGATTCACCCTTAACACA TTGATGTGGCTTGCCATTTTCAAAGAGACGTTTCAGCCAGTATTGTGCAACAAGGAAGTCCCAGTTTCCT CAAGAGAGGGCTACTGTGGCCCATGCCCTAACAACTGGATATGTCACAGAAACAACTGTTACCAATTTTT TAATGAAGAGAAAACCTGGAACCAGAGCCAAGCTTCCTGTTTGTCTCAAAATTCCAGCCTTCTGAAGATA TACAGTAAAGAAGAACAGGATTTCTTAAAGCTGGTTAAGTCCTATCACTGGATGGGACTGGTCCAGATCC CAGCAAATGGCTCCTGGCAGTGGGAAGATGGCTCCTCTCTCTCATACAATCAGTTAACTCTGGTGGAAAT ACCAAAAGGATCCTGTGCTGTCTATGGCTCAAGCTTTAAGGCTTACACAGAAGACTGTGCAAATCTAAAC ACGTACATCTGCATGAAAAGGGCGGTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR222529 representing NM_001083322
Red=Cloning site Green=Tags(s) MSKCHNYDLKPAKWDTSQEQQKQRLALTTSQPGENGIIRGRYPIEKLKISPMFVVRVLAIALAIRFTLNT LMWLAIFKETFQPVLCNKEVPVSSREGYCGPCPNNWICHRNNCYQFFNEEKTWNQSQASCLSQNSSLLKI YSKEEQDFLKLVKSYHWMGLVQIPANGSWQWEDGSSLSYNQLTLVEIPKGSCAVYGSSFKAYTEDCANLN TYICMKRAV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001083322 |
| ORF Size | 657 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001083322.2 |
| RefSeq Size | 3158 bp |
| RefSeq ORF | 660 bp |
| Locus ID | 27007 |
| UniProt ID | O54709 |
| MW | 25.6 kDa |
| Gene Summary | Function as an activating and costimulatory receptor involved in immunosurveillance upon binding to various cellular stress-inducible ligands displayed at the surface of autologous tumor cells and virus-infected cells. Provides both stimulatory and costimulatory innate immune responses on activated killer (NK) cells, leading to cytotoxic activity. Acts as a costimulatory receptor for T-cell receptor (TCR) in CD8(+) T-cell-mediated adaptive immune responses by amplifying T-cell activation. Stimulates perforin-mediated elimination of ligand-expressing tumor cells. Signaling involves calcium influx, culminating in the expression of TNF-alpha. Participates in NK cell-mediated bone marrow graft rejection. May play a regulatory role in differentiation and survival of NK cells. Binds to ligands belonging to various subfamilies of MHC class I-related glycoproteins including RAET1A, RAET1B, RAET1C, RAET1D, RAET1E, H60 and MULT1.[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Nanoscale colocalization of NK cell activating and inhibitory receptors controls signal integration
,Tomaz, D;Pereira, P;Guerra, N;Dyson, J;Henriques, R;Gould, K;,
bioRxiv
[KLRK1]
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Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG222529 | Klrk1 (tGFP-tagged) - Mouse killer cell lectin-like receptor subfamily K member 1 (Klrk1) transcript variant 2, (10ug) |
CNY 2,380.00 |
|
| MR222529L3 | Lenti ORF clone of Klrk1 (Myc-DDK-tagged) - Mouse killer cell lectin-like receptor subfamily K, member 1 (Klrk1), transcript variant 2 |
CNY 4,090.00 |
|
| MR222529L4 | Lenti ORF clone of Klrk1 (mGFP-tagged) - Mouse killer cell lectin-like receptor subfamily K, member 1 (Klrk1), transcript variant 2 |
CNY 4,090.00 |
