United States
Germany
Japan
United Kingdom
China
Jarid2 (NM_021878) Mouse Tagged Lenti ORF Clone
CAT＃: MR222802L3
- LentiORF®
Lenti ORF clone of Jarid2 (Myc-DDK-tagged) - Mouse jumonji, AT rich interactive domain 2 (Jarid2), transcript variant 2
"NM_021878" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 11,500.00
Product images
CNY 600.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Jmj; jumonji |
| Vector | pLenti-C-Myc-DDK-P2A-Puro |
| E. coli Selection | Chloramphenicol (34 ug/mL) |
| Mammalian Cell Selection | Puromycin |
| Sequence Data |
The ORF insert of this clone is exactly the same as(MR222802).
|
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_021878 |
| ORF Size | 3702 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_021878.3, NP_068678.1 |
| RefSeq Size | 5716 bp |
| RefSeq ORF | 3705 bp |
| Locus ID | 16468 |
| UniProt ID | Q62315 |
| Gene Summary | Regulator of histone methyltransferase complexes that plays an essential role in embryonic development, including heart and liver development, neural tube fusion process and hematopoiesis. Acts by modulating histone methyltransferase activity and promoting the recruitment of histone methyltransferase complexes to their target genes. Binds DNA and mediates the recruitment of the PRC2 complex to target genes in embryonic stem cells. Does not have histone demethylase activity but regulates activity of various histone methyltransferase complexes. In embryonic stem cells, it associates with the PRC2 complex and inhibits trimethylation of 'Lys-27' of histone H3 (H3K27me3) by the PRC2 complex, thereby playing a key role in differentiation of embryonic stem cells and normal development. In cardiac cells, it is required to repress expression of cyclin-D1 (CCND1) by activating methylation of 'Lys-9' of histone H3 (H3K9me) by the GLP1/EHMT1 and G9a/EHMT2 histone methyltransferases. Also acts as a transcriptional repressor of ANF via its interaction with GATA4 and NKX2-5. Participates in the negative regulation of cell proliferation signaling.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC224007 | Jarid2 (untagged) - Mouse jumonji, AT rich interactive domain 2 (Jarid2), transcript variant 2, (10ug) |
CNY 15,770.00 |
|
| MG222802 | Jarid2 (tGFP-tagged) - Mouse jumonji AT rich interactive domain 2 (Jarid2), (10ug) |
CNY 10,550.00 |
|
| MR222802 | Jarid2 (Myc-DDK-tagged) - Mouse jumonji, AT rich interactive domain 2 (Jarid2), transcript variant 2 |
CNY 8,592.00 |
|
| MR222802L4 | Lenti ORF clone of Jarid2 (mGFP-tagged) - Mouse jumonji, AT rich interactive domain 2 (Jarid2), transcript variant 2 |
CNY 11,500.00 |
