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Recql5 (NM_130454) Mouse Tagged ORF Clone
CAT＃: MR222846
- TrueORF®
Recql5 (Myc-DDK-tagged) - Mouse RecQ protein-like 5 (Recql5)
ORF Plasmid: tGFP
"NM_130454" in other vectors (4)
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CNY 7,192.00
CNY 8,650.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | RecQ5; Recq5b; Recql5b |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR222846 representing NM_130454
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCGCCCGTCCTTTCTCCACTCCCTTCGACCGGGAGCGGCGAGTCCGGAGCACCCTGAAGAAAGTCT TTGGGTTTGATTCTTTTAAAACACCTTTACAAGAGAGTGCGACCATGGCTGTAGTGAAAGGTGCCGAGGA TGTGTTTGTGTGCATGCCCACAGGGGCAGGGAAGTCTCTGTGCTATCAACTCCCTGCCCTGTTGGCCAGT GGGATCACCATTGTAGTCTCTCCTCTCATTGCCTTGATTCAGGACCAGGTAGACCATTTGCTGGCCTTGA AGGTACAAGTTAGTTCCCTGAACTCAAAGCTCTCGGTACAGGAGAGGAAGGAGCTGCTGTCTGACCTGGA GCGAGACAAACCTCGGACCAAACTTTTGTACATCACCCCAGAGATGGCAGCTTCAGCCTCCTTCCAGCCC ACCCTGAATTCCCTGGTGTCCCGAAACCTGCTCTCCTACTTGGTGGTGGATGAAGCTCATTGCGTTTCCC AGTGGGGACATGACTTCCGTCCTGACTATTTGCGTCTGGGTGCCCTACGTTCCCGCTTGGCACATGCCCC GTGCGTGGCTCTGACGGCTACAGCCACCCCACAGGTTCAAGAGGATGTGTTTGCTGCCCTGCACTTGAAG CAGCCAGTCGCCTCTTTCAAGACTCCCTGTTTCCGGGCCAACCTCTTCTATGATGTGCAGTTCAAGGAAC TGATTCCCGACGTCTATGGCAATCTGAGGGACTTCTGCCTTAAGGCTCTTGGACAGAAGGCTGAGAACGG GTCGTCCTCTGGCTGTGGCATTGTCTACTGCAGGACTAGAGAAGCTTGTGAACAACTGGCCATTGAGCTC AGCAGCAGGGGTGTGAACGCCAAGGCTTACCACGCAGGCCTGAAGGCATCTGATAGAACACAGGTACAGA ACGAGTGGATGGAGGAGAAGGTCCCTGTAATTGTTGCGACCATCAGTTTTGGGATGGGAGTAGACAAAGC TAATGTCCGGTTTGTTGCTCATTGGAATATTGCCAAGTCCATGGCTGGCTACTACCAGGAGTCTGGCCGT GCTGGCAGGGATGGCAAACCATCTTGGTGCCGCCTGTATTACTCTAGGAATGACCGAGACCAAGTCAGCT TCCTTATCAGGAAGGAACTAGCCAAACTCCAGGAAAAGAGGGGGAACAAACCGTCTGATAAGGCCACCCT GCTGGCCTTTGATGCCCTGGTGACCTTCTGTGAAGAAGTGGGGTGCCGCCATGCTGCCATTGCTAAGTAC TTTGGGGATGCACCCCCCGCCTGCGCCAAAGGCTGTGACTACTGCCAGAATCCTGCAGCCATAACGAAGA AGCTGGATGCCCTGGAGCGCAGCAGCAGCTGGAGCAAGACCTGCATCGGGCCCTCACAGGGGAATGGCTT CGATCCTGAGCTGTATGAGGGAGGCCGCAGGGGCTATGGTGGCTTCAGCAGGTATGATGAAGGTTCTGGA GGTAGCGGTGATGAGGGCAGAGATGAGGCCCACAAGCGGGAATGGAATCTTTTCTATCAGAAACAGATGA GCCTGCGCAAGGGCAAAGAGCCCAAGATAGAAGAATTCACGCCCCCAGATGAAGACTGTCCCCTGAGAGA GGCTTCAAGCAGGAAGATCCCGAAGCTCACAGTGAAGGCCCGTGAGCACTGCCTGCGACTTCTGGAGGAG GCTCTGATCAGTAACCATCAGGCTGCAGGCTCCACTCATGGAGCTGACCTGCAGGCCAAGGCTGTGGAAC TGGAGCATGAGACATTCCGGAATGCCAAGATGGTCAATCTGTACAAGGCCAGTGTCCTGAAGAAGGTGGC CGAGATCCACAAAGCCTCCAAGGATGGTCAGCTCTATGACATGGAAAGCGGCACCAAGAGCTGTGGGGCC GCAGCTGAGTTCTCGGAGCCCAGTGACTATGACATCCCGCCAACCTCACATGTGTACTCGCTCAAACCCA AGAGAGTAGGAGCTGGCTTCTCCAAAGGCCCCTGCTCATTCCAGACAGCCACAGAACTGTTGGGGAAGTC TCACTCCCAGAAGCAAGCCCCAGAGGCTATGCTAGAGGGTGGACAGGAGCCCCCAGGCTGGGTCTGTGAC CTTCAAGATGAAGACAGGAGCAAGCCCCACCCTGGGTACCAAGAAAAGGCTCTAGGAAGCAGTGTTAATT GTGGGGACCCCTCGCCTGAGAAGAAGACGAAAGGGTCTTCCCAGGGCAGTGCCAAAGCTAGAGCCAGCAA GAAACAACAGCTCCTAGCCACAGCAGCCCGCAAGGATTCCCAGAATATCACTCGATTCCTCTGTCAGAGG ACAGAATCTCCACCTCTCCCTGCTTCAGTTCCAAGATCAGAAGATGCCAGCCCCTCCTGTGGGGATGTCC CAGGGAAGTGCACACAAGAGGTTGGAGCTCAGGGACATTTGGTTGCTGTATTTCAGACTGAGGGTCCCAG GGAGAGGCCAAGCACCTGCTCACTCAGAGACCAGAGCTTCCCTGAAGGTCAGCCTAGCCCTCTAAAGGAA ACCCAGGCAGAGAAGCGGCCCAGACCACAGCAGGGCAACCCAGAGAGAAGGGCTCAAAAGAGGCTTCGCC CCTCAACCAAGTCCTCCATCTTGGCTGAGGCCAAGGACAGTACCTTGGCCAGTGATCGCAGCACTGAAAA CAAAGTGGCTCAAGAACCCTGCCAGCTCTCAGCATCTGGCACCTCCCTGAGGGAGGCCGCAGACATTGTG GTCAGGCACCTGACCCCCTTCTACAAGGAGGGCAGGTTTATATCCAAGGACCTGTTTAAGGGCTTTGCCC GCCACCTCTCACATTTGCTGGCTCAGCAGCTCTCTCCTGGAAGGAGTGTGAAGGAAGAGGCCCAGAGCCT CATCAAGCAGTTCTTCCACAATCGTGCCCGCTGTGAGAGTGAAGCTGACTGGCACAGCCTGCGTGGCCCA CAGCGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR222846 representing NM_130454
Red=Cloning site Green=Tags(s) MSARPFSTPFDRERRVRSTLKKVFGFDSFKTPLQESATMAVVKGAEDVFVCMPTGAGKSLCYQLPALLAS GITIVVSPLIALIQDQVDHLLALKVQVSSLNSKLSVQERKELLSDLERDKPRTKLLYITPEMAASASFQP TLNSLVSRNLLSYLVVDEAHCVSQWGHDFRPDYLRLGALRSRLAHAPCVALTATATPQVQEDVFAALHLK QPVASFKTPCFRANLFYDVQFKELIPDVYGNLRDFCLKALGQKAENGSSSGCGIVYCRTREACEQLAIEL SSRGVNAKAYHAGLKASDRTQVQNEWMEEKVPVIVATISFGMGVDKANVRFVAHWNIAKSMAGYYQESGR AGRDGKPSWCRLYYSRNDRDQVSFLIRKELAKLQEKRGNKPSDKATLLAFDALVTFCEEVGCRHAAIAKY FGDAPPACAKGCDYCQNPAAITKKLDALERSSSWSKTCIGPSQGNGFDPELYEGGRRGYGGFSRYDEGSG GSGDEGRDEAHKREWNLFYQKQMSLRKGKEPKIEEFTPPDEDCPLREASSRKIPKLTVKAREHCLRLLEE ALISNHQAAGSTHGADLQAKAVELEHETFRNAKMVNLYKASVLKKVAEIHKASKDGQLYDMESGTKSCGA AAEFSEPSDYDIPPTSHVYSLKPKRVGAGFSKGPCSFQTATELLGKSHSQKQAPEAMLEGGQEPPGWVCD LQDEDRSKPHPGYQEKALGSSVNCGDPSPEKKTKGSSQGSAKARASKKQQLLATAARKDSQNITRFLCQR TESPPLPASVPRSEDASPSCGDVPGKCTQEVGAQGHLVAVFQTEGPRERPSTCSLRDQSFPEGQPSPLKE TQAEKRPRPQQGNPERRAQKRLRPSTKSSILAEAKDSTLASDRSTENKVAQEPCQLSASGTSLREAADIV VRHLTPFYKEGRFISKDLFKGFARHLSHLLAQQLSPGRSVKEEAQSLIKQFFHNRARCESEADWHSLRGP QR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_130454 |
| ORF Size | 2946 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_130454.2, NP_569721.1 |
| RefSeq Size | 4001 bp |
| RefSeq ORF | 2949 bp |
| Locus ID | 170472 |
| UniProt ID | Q8VID5 |
| MW | 108.2 kDa |
| Gene Summary | DNA helicase that plays an important role in DNA replication, transcription and repair. Inhibits elongation of stalled transcripts at DNA damage sites by binding to the RNA polymerase II subunit POLR2A and blocking the TCEA1 binding site. Required for mitotic chromosome separation after cross-over events and cell cycle progress. Required for efficient DNA repair, including repair of inter-strand cross-links. Stimulates DNA decatenation mediated by TOP2A. Prevents sister chromatid exchange and homologous recombination (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC222962 | Recql5 (untagged) - Mouse RecQ protein-like 5 (Recql5), (10ug) |
CNY 12,640.00 |
|
| MG222846 | Recql5 (tGFP-tagged) - Mouse RecQ protein-like 5 (Recql5), (10ug) |
CNY 9,500.00 |
|
| MR222846L3 | Lenti ORF clone of Recql5 (Myc-DDK-tagged) - Mouse RecQ protein-like 5 (Recql5) |
CNY 10,550.00 |
|
| MR222846L4 | Lenti ORF clone of Recql5 (mGFP-tagged) - Mouse RecQ protein-like 5 (Recql5) |
CNY 10,550.00 |
