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Piwil1 (NM_021311) Mouse Tagged ORF Clone
CAT＃: MR222848
- TrueORF®
Piwil1 (Myc-DDK-tagged) - Mouse piwi-like homolog 1 (Drosophila) (Piwil1)
ORF Plasmid: tGFP
"NM_021311" in other vectors (4)
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CNY 6,320.00
CNY 7,130.00
Product images
CNY 300.00
热销推荐Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | MIWI |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR222848 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACTGGCCGAGCCCGAGCTCGGGCCCGCGGCAGGGCACGAGGTCAGGAGACGGTGCAGCATGTTGGGG CTGCTGCGAGCCAGCAACCTGGGTACATCCCACCGAGACCTCAACAGTCCCCCACAGAGGGGGACTTGGT TGGCCGAGGACGACAGAGGGGGATGGTAGTCGGAGCCACATCCAAGTCACAAGAACTGCAGATCTCAGCT GGGTTTCAGGAGCTGTCACTGGCAGAGAGAGGAGGGCGTCGCCGAGACTTCCATGACCTTGGTGTGAACA CCAGACAGAACCTTGACCATGTCAAAGAGTCAAAGACAGGCTCCTCTGGCATCATTGTGAAGCTGAGCAC CAACCACTTCCGGCTGACCTCGCGCCCACAGTGGGCCCTGTATCAGTACCACATCGACTACAATCCCCTG ATGGAGGCCCGAAGGCTTCGCTCCGCACTGCTCTTCCAGCATGAAGACCTCATTGGAAGGTGTCATGCTT TCGATGGGACAATATTGTTTTTACCTAAGAGACTACAGCACAAGGTCACAGAAGTATTCAGTCAGACTCG GAATGGGGAACACGTGAGGATCACCATCACCCTGACCAACGAGCTGCCGCCCACCTCGCCCACCTGCCTG CAGTTCTATAACATCATCTTCAGGAGGCTCTTGAAAATCATGAATTTGCAACAAATTGGACGGAATTATT ACAATCCAAGTGACCCGATTGATATTCCAAACCACAGGTTGGTGATCTGGCCCGGCTTCACCACCTCCAT CCTTCAGTATGAGAACAACATCATGCTCTGCACAGACGTCAGCCACAAGGTGCTCCGCAGCGAGACTGTC CTAGACTTCATGTTCAATCTATACCAGCAGACAGAGGAGCACAAGTTCCAGGAGCAAGTGTCGAAGGAGC TCATAGGCCTCATCGTTCTCACCAAGTACAATAACAAGACCTACCGGGTGGATGACATTGACTGGGACCA GAATCCAAAGAGCACCTTCAAGAAGGCGGATGGCTCGGAGGTCAGCTTCCTGGAGTACTACAGGAAGCAA TACAACCAGGAGATCACGGACCTGAAGCAGCCGGTGCTGGTGAGCCAACCCAAGCGGAGGAGAGGCCCCG GCGGCACCCTGCCTGGCCCAGCTATGCTCATCCCTGAACTCTGCTATCTCACAGGCCTGACTGATAAAAT GCGCAATGATTTCAATGTGATGAAGGACCTGGCAGTGCACACGCGGCTGACCCCTGAGCAGCGGCAGCGG GAGGTGGGCCGCCTCATCGACTACATCCACAAGGATGACAATGTGCAGAGAGAGCTTCGAGACTGGGGCC TGAGCTTCGACTCAAACTTGCTGTCCTTCTCTGGAAGAATCTTACAATCTGAGAAGATCCACCAGGGCGG AAAGACGTTTGATTACAACCCACAATTTGCAGACTGGTCCAAAGAAACAAGAGGCGCGCCGCTGATCAGC GTGAAGCCATTGGATAACTGGCTGCTGATCTATACCCGCAGGAATTATGAAGCAGCCAACTCACTGATAC AGAACCTGTTCAAAGTGACTCCAGCCATGGGCATCCAGATGAAAAAGGCAATCATGATCGAGGTGGATGA CAGAACAGAAGCTTATCTGAGAGCCTTGCAGCAGAAGGTGACGTCAGACACTCAGATAGTTGTCTGTCTC TTGTCAAGTAATCGGAAGGACAAATATGATGCCATCAAGAAGTACTTGTGTACAGACTGCCCCACCCCAA GTCAGTGTGTGGTGGCCCGGACCCTGGGCAAGCAGCAAACAGTCATGGCCATTGCCACCAAGATCGCCCT GCAGATGAACTGCAAGATGGGAGGCGAGCTCTGGCGGGTGGACATGCCCCTGAAACTGGCAATGATCGTG GGCATCGACTGTTACCATGACACCACAGCTGGGCGGAGGTCCATCGCAGGATTCGTCGCCAGCATCAATG AAGGGATGACCCGCTGGTTCTCCCGCTGCGTCTTTCAGGACCGCGGGCAGGAGCTGGTGGATGGTCTCAA GGTGTGCTTGCAAGCTGCTCTGAGGGCTTGGAGTGGCTGCAATGAATACATGCCCAGCCGTGTCATCGTG TACCGAGACGGTGTGGGGGACGGGCAGCTGAAGACCCTGGTCAATTATGAGGTCCCACAGTTCCTAGATT GCCTCAAGTCAGTCGGGAGAGGTTACAACCCAAGACTGACTGTAATCGTGGTGAAGAAGCGTGTCAATGC CAGGTTTTTTGCTCAGTCTGGGGGAAGACTTCAGAACCCTCTTCCAGGGACAGTCATCGATGTGGAAGTC ACCAGACCAGAGTGGTATGACTTTTTCATCGTGAGCCAGGCAGTGAGAAGCGGGAGTGTGTCCCCAACAC ACTACAATGTCATCTATGACAGCAGTGGCCTGAAGCCCGACCACATCCAGCGGCTGACATACAAGCTCTG CCACGTGTACTATAATTGGCCTGGAGTCATCCGAGTCCCTGCACCTTGCCAGTATGCACACAAGCTGGCC TTCCTCGTGGGCCAGAGCATCCACAGAGAGCCAAACCTCTCCCTGTCCAACCGCCTCTACTACCTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR222848 protein sequence
Red=Cloning site Green=Tags(s) MTGRARARARGRARGQETVQHVGAAASQQPGYIPPRPQQSPTEGDLVGRGRQRGMVVGATSKSQELQISA GFQELSLAERGGRRRDFHDLGVNTRQNLDHVKESKTGSSGIIVKLSTNHFRLTSRPQWALYQYHIDYNPL MEARRLRSALLFQHEDLIGRCHAFDGTILFLPKRLQHKVTEVFSQTRNGEHVRITITLTNELPPTSPTCL QFYNIIFRRLLKIMNLQQIGRNYYNPSDPIDIPNHRLVIWPGFTTSILQYENNIMLCTDVSHKVLRSETV LDFMFNLYQQTEEHKFQEQVSKELIGLIVLTKYNNKTYRVDDIDWDQNPKSTFKKADGSEVSFLEYYRKQ YNQEITDLKQPVLVSQPKRRRGPGGTLPGPAMLIPELCYLTGLTDKMRNDFNVMKDLAVHTRLTPEQRQR EVGRLIDYIHKDDNVQRELRDWGLSFDSNLLSFSGRILQSEKIHQGGKTFDYNPQFADWSKETRGAPLIS VKPLDNWLLIYTRRNYEAANSLIQNLFKVTPAMGIQMKKAIMIEVDDRTEAYLRALQQKVTSDTQIVVCL LSSNRKDKYDAIKKYLCTDCPTPSQCVVARTLGKQQTVMAIATKIALQMNCKMGGELWRVDMPLKLAMIV GIDCYHDTTAGRRSIAGFVASINEGMTRWFSRCVFQDRGQELVDGLKVCLQAALRAWSGCNEYMPSRVIV YRDGVGDGQLKTLVNYEVPQFLDCLKSVGRGYNPRLTVIVVKKRVNARFFAQSGGRLQNPLPGTVIDVEV TRPEWYDFFIVSQAVRSGSVSPTHYNVIYDSSGLKPDHIQRLTYKLCHVYYNWPGVIRVPAPCQYAHKLA FLVGQSIHREPNLSLSNRLYYL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_021311 |
| ORF Size | 2589 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_021311.3, NP_067286.1 |
| RefSeq Size | 3944 bp |
| RefSeq ORF | 2589 bp |
| Locus ID | 57749 |
| UniProt ID | Q9JMB7 |
| MW | 98.6 kDa |
| Gene Summary | Endoribonuclease that plays a central role in postnatal germ cells by repressing transposable elements and preventing their mobilization, which is essential for the germline integrity (PubMed:11578866, PubMed:22121019, PubMed:21237665). Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons (PubMed:11578866, PubMed:22121019, PubMed:21237665). Directly binds methylated piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements (PubMed:11578866, PubMed:22121019, PubMed:21237665). Strongly prefers a uridine in the first position of their guide (g1U preference, also named 1U-bias) (PubMed:24757166). Not involved in the piRNA amplification loop, also named ping-pong amplification cycle (PubMed:22121019). Acts as an endoribonuclease that cleaves transposon messenger RNAs (PubMed:22121019). Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation (PubMed:16938833). Probable component of some RISC complex, which mediates RNA cleavage and translational silencing (PubMed:16938833). Also plays a role in the formation of chromatoid bodies and is required for some miRNAs stability (PubMed:16787948). Required to sequester RNF8 in the cytoplasm until late spermatogenesis; RNF8 being released upon ubiquitination and degradation of PIWIL1 (PubMed:28552346).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC222266 | Piwil1 (untagged) - Mouse piwi-like homolog 1 (Drosophila) (Piwil1), (10ug) |
CNY 6,320.00 |
|
| MG222848 | Piwil1 (tGFP-tagged) - Mouse piwi-like homolog 1 (Drosophila) (Piwil1), (10ug) |
CNY 7,890.00 |
|
| MR222848L3 | Lenti ORF clone of Piwil1 (Myc-DDK-tagged) - Mouse piwi-like homolog 1 (Drosophila) (Piwil1) |
CNY 9,030.00 |
|
| MR222848L4 | Lenti ORF clone of Piwil1 (mGFP-tagged) - Mouse piwi-like homolog 1 (Drosophila) (Piwil1) |
CNY 9,030.00 |
