Cacna1d (NM_001083616) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	8430418G19Rik; C79217; Cach3; Cacn; Cacn4; Cacnl1a2; Cav1.3; Cchl; Cchl1; Cchl1a; Cchl1a2; D-LTC; D-LTCC
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR222926 representing NM_001083616 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGATGATGATGATGATGAAAAAAATGCAACATCAACGGCAGCACCAAGAGGACCATGCGAACGAGG CAAACTATGCAAGAGGCACCAGACTCCCTATTTCTGGTGAAGGACCAACTTCTCAGCCCAACAGCTCCAA GCAAACTGTCCTATCTTGGCAAGCTGCAATCGATGCTGCTAGACAGGCCAAGGCCGCCCAGACCATGAGC ACCTCTGCACCCCCACCTGTAGGATCTCTCTCCCAAAGAAAACGTCAGCAATACGCCAAGAGCAAAAAAC AGGGTAACTCGTCTAACAGCCGACCTGCCCGTGCCCTTTTCTGTTTATCCCTCAATAACCCCATCCGAAG AGCCTGCATTAGTATAGTGGAATGGAAACCATTTGACATATTTATATTATTGGCTATTTTTGCCAATTGT GTGGCCTTAGCTATTTACATCCCATTCCCTGAAGATGATTCTAATTCAACAAATCATAACTTGGAAAAAG TAGAATATGCCTTCCTGATTATTTTTACAGTGGAGACATTTCTGAAGATTATAGCGTATGGACTGTTGCT GCATCCTAATGCTTACGTTAGGAATGGATGGAATTTACTGGATTTTGTTATAGTAATTGTAGGATTGTTT AGTGTAATTCTGGAACAATTAACCAAAGAAACAGAAGGCGGGAACCACTCTAGTGGCAAATCAGGAGGCT TTGATGTCAAAGCCCTCCGTGCCTTCCGAGTGTTAAGACCACTTCGACTTGTATCAGGAGTGCCCAGTTT ACAAGTTGTTCTGAACTCCATTATAAAAGCCATGGTCCCCCTCCTTCACATAGCCCTTTTGGTATTATTT GTAATTATCATCTATGCTATTATAGGATTGGAACTTTTTATTGGAAAAATGCACAAAACATGTTTTTTTG CTGACTCAGATATAGTAGCTGAAGAGGACCCAGCTCCGTGTGCGTTCTCAGGGAATGGACGCCAGTGCAC CGCCAATGGCACGGAATGTAGGAGTGGCTGGGTTGGCCCCAATGGAGGCATCACTAACTTTGATAACTTT GCCTTTGCCATGCTCACTGTGTTCCAGTGCATTACCATGGAGGGCTGGACAGATGTGCTCTACTGGGTTA ATGATGCGATAGGATGGGAATGGCCATGGGTGTATTTTGTTAGTCTGATCATCCTTGGCTCATTTTTCGT CCTTAACCTGGTTCTTGGTGTCCTTAGTGGAGAATTCTCCAAGGAAAGAGAGAAGGCTAAAGCTCGTGGA GATTTCCAGAAGCTTCGGGAAAAACAGCAGCTGGAAGAGGACCTCAAGGGCTACCTGGACTGGATCACCC AGGCAGAAGACATCGATCCCGAGAACGAGGAGGAGGGTGGCGAGGAAGGCAAACGAAACACTAGCATGCC CACCAGCGAGACTGAATCTGTCAACACAGAGAACGTAAGTGGTGAAGGCGAGACCCAGGGATGCTGTGGA ACTCTCTGTCAGGCCATCTCGAAATCCAAACTCAGCCGTCGATGGCGTCGCTGGAACCGGTTCAATCGCA GGAGGTGCAGGGCTGCCGTGAAGTCTGTCACGTTTTACTGGCTGGTGATTGTCCTGGTGTTTCTCAACAC TTTAACTATTTCCTCTGAGCATTACAATCAGCCAGACTGGCTGACACAGATTCAAGACATCGCAAACAAA GTCCTCTTGGCTCTGTTCACCTGTGAGATGCTGGTGAAGATGTACAGCCTGGGCCTCCAGGCTTACTTTG TCTCCCTCTTCAACCGCTTCGATTGCTTTGTGGTGTGTGGGGGCATCACCGAAACCATCCTGGTGGAACT GGAGCTCATGTCTCCCCTGGGGGTCTCCGTGTTCCGGTGTGTGCGCCTCCTGAGAATCTTCAAAGTGACC AGGCACTGGACCTCCCTGAGCAACTTGGTGGCATCCTTGTTAAACTCCATGAAGTCCATCGCCTCTCTGC TGTTACTCCTCTTCCTCTTCATCATCATCTTCTCCTTGCTGGGGATGCAGTTGTTTGGTGGGAAGTTTAA TTTTGACGAGACTCAAACCAAGCGAAGCACCTTTGACAACTTTCCGCAAGCACTCCTGACGGTGTTCCAG ATCCTGACAGGTGAAGACTGGAATGCGGTGATGTATGACGGCATCATGGCCTATGGAGGCCCTTCCTCTT CCGGGATGATTGTCTGTATCTACTTCATCATCCTTTTCATCTGTGGTAACTATATCCTGCTGAATGTCTT CTTGGCCATCGCTGTGGACAATTTGGCTGATGCTGAAAGTCTGAACACTGCTCAGAAAGAGGAAGCTGAA GAAAAAGAAAGGAAAAAGATTGCCAGAAAAGAAAGCCTAGAAAACAAAAAGAACAACAAACCAGAAGTCA ACCAGATAGCCAACAGTGACAACAAGGTTACAATTGATGATTATCAGGAAGATGCTGAAGACAAGGACCC TTACCCGCCCTGTGATGTGCCAGTAGGTGAAGAGGAAGAGGAGGAGGAAGAGGATGAGCCCGAAGTTCCT GCTGGTCCCCGCCCTCGCAGAATCTCAGAGTTGAACATGAAGGAGAAGATTGCGCCCATCCCAGAAGGAA GCGCCTTCTTCATTCTTAGCAAGACCAACCCGATACGTGTGGGTTGCCACAAGCTCATTAACCACCACAT CTTCACCAACCTTATCCTGGTCTTCATCATGCTGAGCAGCGCTGCCCTGGCTGCAGAGGACCCCATCCGT AGCCACTCATTCCGCAACACGATACTGGGCTACTTTGACTATGCCTTCACAGCCATCTTTACGGTTGAAA TCCTGTTAAAGATGACAACTTTTGGAGCCTTCTTGCACAAAGGGGCTTTCTGCAGGAACTACTTCAATTT GCTGGACATGCTGGTTGTTGGGGTGTCGCTGGTGTCCTTTGGGATTCAGTCCAGCGCCATCTCCGTTGTG AAGATTCTGAGGGTTTTGAGGGTCTTGCGGCCTCTCAGAGCAATCAACAGAGCAAAGGGACTTAAGCACG TGGTCCAGTGTGTCTTTGTGGCCATCCGAACCATTGGCAACATCATGATCGTCACGACCCTGCTCCAGTT CATGTTTGCATGCATTGGGGTCCAGCTGTTCAAGGGGAAGTTCTACCGTTGCACAGATGAAGCCAAAAGT AACCCTGAGGAGTGCAGGGGGCTTTTCATTCTTTATAAGGACGGCGATGTTGACAGTCCTGTGGTCCGTG AGAGGATCTGGCAAAATAGTGATTTCAATTTTGACAATGTCCTTTCGGCTATGATGGCGCTGTTCACAGT CTCAACTTTTGAGGGCTGGCCCGCGTTGCTGTACAAAGCTATTGATTCAAACGGTGAGAACGTTGGTCCT GTCTACAACTACCGTGTGGAGATCTCCATCTTCTTCATCATCTACATCATCATCGTTGCCTTCTTCATGA TGAACATCTTTGTGGGCTTCGTCATCGTCACCTTCCAGGAACAGGGAGAAAAAGAGTATAAGAACTGTGA GCTGGACAAAAATCAGCGTCAGTGTGTTGAATATGCCTTGAAAGCACGCCCCTTAAGGAGATACATCCCC AAAAACCCATACCAGTACAAGTTCTGGTATGTGGTGAACTCCTCGCCTTTCGAATACATGATGTTCGTCC TCATCATGCTCAACACGCTCTGCCTGGCCATGCAGCACTATGAGCAGTCCAAGATGTTCAATGATGCCAT GGACATTCTGAACATGGTCTTCACAGGGGTCTTCACTGTTGAGATGGTTTTGAAAGTCATTGCTTTCAAG CCCAAGGGGTATTTTAGTGACGCCTGGAACACGTTTGACTCCCTCATCGTAATCGGCAGCATTATAGACG TGGCCCTCAGCGAAGCCGACAACTCTGAAGAGAGCAATAGAATCTCCATCACCTTTTTCCGTCTTTTCCG AGTGATGCGGTTGGTGAAACTTCTCAGCAGGGGGGAAGGCATCCGGACACTGCTGTGGACCTTCATCAAG TCCTTTCAGGCGCTGCCGTATGTTGCCCTCCTCATTGCCATGCTCTTCTTCATCTACGCTGTCATAGGCA TGCAGATGTTTGGGAAAGTTGCCATGAGAGATAACAACCAGATCAATAGGAACAACAACTTCCAGACCTT TCCTCAGGCAGTGCTCCTACTCTTCAGGTGTGCAACAGGGGAAGCCTGGCAGGAGATCATGCTTGCCTGC CTCCCAGGAAAGCTGTGCGACCCAGACTCCGATTACAACCCAGGAGAGGAGTACACTTGTGGGAGCAACT TTGCCATTGTCTACTTCATCAGCTTTTACATGCTCTGTGCATTCCTGATCATCAACCTCTTTGTGGCTGT CATCATGGACAATTTTGACTATCTGACTCGGGACTGGTCTATTCTGGGGCCTCACCACTTGGACGAATTC AAAAGAATATGGTCAGAATATGACCCTGAAGCAAAGGGAAGGATAAAACACCTTGATGTGGTCACTCTGC TCCGGCGGATCCAGCCTCCCCTGGGGTTTGGAAAATTATGCCCACACCGAGTAGCATGTAAGAGATTGGT TGCCATGAACATGCCTCTCAACAGTGATGGGACAGTCATGTTCAATGCAACCCTGTTTGCTTTGGTCCGG ACAGCTCTCAAGATCAAGACTGAAGGGAACCTGGAGCAAGCTAATGAAGAACTCCGAGCTGTGATCAAGA AAATCTGGAAGAAGACAAGCATGAAGCTGCTTGACCAAGTTGTCCCTCCAGCTGGTGATGATGAGGTAAC CGTGGGGAAGTTCTATGCCACTTTCCTGATACAGGACTACTTTAGGAAATTCAAGAAACGGAAAGAGCAA GGACTGGTGGGGAAGTACCCTGCGAAGAACACCACGATTGCCCTACAGGCGGGATTAAGGACCCTGCATG ACATTGGGCCAGAAATCCGACGGGCTATATCCTGTGACTTGCAAGATGACGAGCCAGAAGACTCCAAACC AGAAGAAGAAGATGTATTCAAAAGAAATGGTGCCCTGCTTGGAAACCATGTCAATCATGTTAATAGTGAT AGGAGAGATTCCCTTCAGCAGACCAATACCACCCACCGTCCCCTGCATGTCCAAAGGCCTTCAATGCCAC CTGCAAGTGATACTGAGAAACCGCTGTTTCCTCCAGCAGGAAATTCGGGGTGTCATAACCATCATAACCA TAATTCCATAGGGAAGCAAGCTCCCACCTCAACAAATGCCAATCTCAATAATGCCAATATGTCCAAAGCT GCCCACGGAAAGCCGCCCAGCATTGGGAACCTTGAGCATGTGTCTGAAAATGGGCATTACTCCTGCAAGC ATGATCGAGAGCTTCAAAGAAGGTCCAGTATTAAAAGAACCCGCTATTACGAGACTTACATTAGGTCTGA GTCAGGAGATGAGCAATTTCCAACCATTTGCCGTGAAGATCCTGAGATACACGGCTACTTCCGAGACCCC CGCTGCTTGGGGGAACAGGAGTATTTCAGTAGCGAGGAGTGCTGTGAGGACGACAGCTCTCCCACCTGGA GCAGGCAAAACTACAACTACTACAACAGGTACCCAGGCAGCTCCATGGACTTTGAGAGGCCCCGAGGCTA CCACCACCCCCAAGGCTTCTTAGAAGATGATGACTCTCCCACTGGCTATGATTCACGGAGATCTCCGAGG AGACGTCTTCTACCTCCTACCCCACCATCCCATCGGAGGTCCTCCTTCAACTTTGAGTGTCTGCGCAGGC AAAGCAGCCAAGACGATGTCCTTCCATCGCCTGCCCTGCCTCACCGCGCTGCCCTGCCCCTGCACCTGAT GCAGCAGCAGATCATGGCCGTTGCAGGCCTAGATTCCAGTAAAGCCCAGAAGTACTCACCGAGCCACTCT ACCCGGTCGTGGGCCACCCCTCCAGCAACCCCTCCATACCGAGACTGGAGCCCATGCTACACCCCTTTGA TCCAGGTGGACCGCTCAGAATCTATGGACCAGGTCAATGGCAGCCTGCCATCCCTACACCGAAGCTCCTG GTACACAGATGAACCCGACATCTCCTATAGGACTTTCACGCCAGCTAGTCTGACTGTCCCCAGCAGCTTC CGGAACAAGAACAGCGACAAGCAGAGGAGTGCAGACAGCCTGGTGGAGGCAGTCCTGATATCCGAAGGCT TGGGACGCTACGCGAGGGACCCCAAATTTGTCTCAGCGACAAAACATGAGATTGCCGATGCCTGTGACCT AACCATAGACGAGATGGAAAGTGCTGCCAGCACCCTGCTCAATGGCAGTGTGTGTCCTCGAGCCAATGGG GACATGGGCCCCATCTCCCACCGCCAGGACTATGAGCTCCAGGACTTTGGTCCTGGCTACAGTGACGAGG AGCCAGACCCTGGGCGAGAGGAAGAGGACCTGGCAGATGAGATGATTTGCATTACCACCTTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR222926 representing NM_001083616 Red=Cloning site Green=Tags(s) MMMMMMMKKMQHQRQHQEDHANEANYARGTRLPISGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMS TSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANC VALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLF SVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLF VIIIYAIIGLELFIGKMHKTCFFADSDIVAEEDPAPCAFSGNGRQCTANGTECRSGWVGPNGGITNFDNF AFAMLTVFQCITMEGWTDVLYWVNDAIGWEWPWVYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARG DFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEEGKRNTSMPTSETESVNTENVSGEGETQGCCG TLCQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANK VLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELELMSPLGVSVFRCVRLLRIFKVT RHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQ ILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAE EKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYQEDAEDKDPYPPCDVPVGEEEEEEEEDEPEVP AGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIR SHSFRNTILGYFDYAFTAIFTVEILLKMTTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVV KILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKS NPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGP VYNYRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIP KNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFK PKGYFSDAWNTFDSLIVIGSIIDVALSEADNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIK SFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLAC LPGKLCDPDSDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEF KRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVR TALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQ GLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEDSKPEEEDVFKRNGALLGNHVNHVNSD RRDSLQQTNTTHRPLHVQRPSMPPASDTEKPLFPPAGNSGCHNHHNHNSIGKQAPTSTNANLNNANMSKA AHGKPPSIGNLEHVSENGHYSCKHDRELQRRSSIKRTRYYETYIRSESGDEQFPTICREDPEIHGYFRDP RCLGEQEYFSSEECCEDDSSPTWSRQNYNYYNRYPGSSMDFERPRGYHHPQGFLEDDDSPTGYDSRRSPR RRLLPPTPPSHRRSSFNFECLRRQSSQDDVLPSPALPHRAALPLHLMQQQIMAVAGLDSSKAQKYSPSHS TRSWATPPATPPYRDWSPCYTPLIQVDRSESMDQVNGSLPSLHRSSWYTDEPDISYRTFTPASLTVPSSF RNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASTLLNGSVCPRANG DMGPISHRQDYELQDFGPGYSDEEPDPGREEEDLADEMICITTL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001083616
ORF Size	6432 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001083616.2, NP_001077085.1
RefSeq Size	9079 bp
RefSeq ORF	6435 bp
Locus ID	12289
UniProt ID	Q99246
MW	243.6 kDa
Gene Summary	This gene encodes a pore-forming subunit of the L-type, voltage-activated calcium channel family. These channels have been found to play a role in heart and smooth muscle contraction and in the transmission of auditory information. Homozygous knockout mice for this gene exhibit deafness and heart defects. These channels have also been linked to mitochondrial oxidative stress in a mouse model of Parkinson's disease. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Nov 2014]

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