Cacna1e (NM_009782) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	A430040I15; alp; alpha1E; BII; Cach6; Cacnl1a6; Cav2; Cav2.3; Cchr; Cchra1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR223012 representing NM_009782 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTCGCTTCGGGGAGGCGGTGGTCGTTGGCAGGCCAGGCTCAGGCGATGGAGACTCGGACCAGAGCA GGAACCGACAAGGAACCCCCGTCCCGGCCTCGGGGCCGGCGGCCGCCTACAAGCAGTCAAAAGCGCAGAG GGCGCGGACTATGGCTTTGTACAACCCCATACCAGTCCGGCAGAACTGTTTCACGGTCAACAGATCCCTG TTCATCTTCGGAGAAGATAACATTGTCAGGAAATACGCCAAGAAGCTCATCGATTGGCCGCCATTTGAGT ACATGATCCTGGCCACCATCATTGCCAACTGCATCGTCTTGGCCCTGGAGCAGCATCTTCCTGAGGATGA CAAGACCCCAATGTCTCGAAGACTGGAGAAGACAGAACCATATTTCATTGGGATCTTCTGCTTTGAAGCT GGGATCAAAATTGTGGCTCTAGGGTTCATCTTCCATAAGGGTTCATACCTCCGTAATGGCTGGAATGTCA TGGACTTCATCGTGGTCCTTAGTGGCATCCTGGCCACTGCGGGAACCCACTTCAACACCCACGTGGACCT GCGGACCCTCCGGGCTGTGCGTGTCCTGAGGCCTTTAAAGCTGGTATCAGGAATACCTAGCCTGCAGATC GTGCTGAAGTCTATCATGAAGGCCATGGTGCCTCTTCTGCAGATTGGCCTTCTGCTCTTCTTTGCCATCC TCATGTTTGCTATCATTGGCTTGGAGTTCTACAGTGGCAAGTTGCATCGAGCATGCTTCATGAACAATTC AGGTATTCTAGAGGGGTTTGATCCTCCTCACCCGTGTGGTGTGCAAGGCTGCCCGGCTGGTTATGAATGT AAGGACTGGATCGGCCCCAATGACGGGATCACCCAGTTTGACAACATCCTTTTTGCCGTGCTGACTGTCT TCCAATGCATCACTATGGAAGGGTGGACCACTGTGCTGTACAATACCAATGATGCCTTAGGAGCCACCTG GAACTGGCTGTACTTCATCCCTCTCATCATCATCGGATCCTTCTTTGTTCTCAACCTCGTCCTGGGAGTG CTTTCCGGGGAATTTGCCAAAGAAAGAGAGAGAGTGGAGAACCGAAGAGCGTTCATGAAGCTCCGGCGCC AGCAACAGATTGAGCGAGAATTGAACGGCTACCGTGCCTGGATAGACAAAGCAGAGGAAGTCATGCTTGC TGAAGAAAATAAAAACTCGGGAACATCAGCCTTGGAAGTGCTTCGAAGGGCAACCATCAAAAGGAGCCGG ACGGAGGCCATGACCCGAGACTCCAGCGATGAGCACTGCGTTGATATCTCTTCAGTGGGCACACCTCTTG CCAGAGCCAGTATCAAGAGCACAAAAGTAGATGGGGCCTCCTATTTCCGGCACAAGGAACGGCTTCTGCG CATCTCTATCCGCCACATGGTCAAGTCCCAAGTCTTTTACTGGATCGTCCTGAGTGTTGTGGCTCTTAAT ACTGCCTGTGTGGCCATTGTTCATCACAACCAGCCACAGTGGCTCACTCACCTCCTCTACTATGCAGAGT TTTTGTTTCTGGGACTCTTCCTGTTGGAGATGTCCCTGAAGATGTATGGCATGGGGCCACGCCTCTATTT TCACTCATCCTTCAACTGCTTTGATTTTGGGGTCACAGTGGGCAGCATCTTTGAGGTTGTCTGGGCAATC TTCAGACCTGGCACATCTTTTGGAATCAGTGTCTTACGAGCTCTCCGGCTCCTAAGGATATTTAAAATAA CCAAGTATTGGGCATCCCTCCGGAATCTGGTTGTCTCCTTGATGAGCTCCATGAAGTCTATCATCAGCTT ACTCTTCCTTCTCTTCCTCTTCATCGTTGTCTTTGCTCTCCTGGGAATGCAGCTCTTTGGAGGCAGGTTT AACTTTAATGATGGGACTCCTTCAGCTAATTTTGACACCTTCCCTGCAGCTATCATGACAGTGTTCCAGA TCCTGACAGGGGAAGACTGGAATGAGGTGATGTACAATGGCATTCGCTCCCAGGGTGGGGTCAGCTCAGG CATGTGGTCTGCCATCTACTTCATTGTGCTCACCTTGTTTGGAAACTACACGCTGCTAAATGTATTCTTG GCCATCGCTGTGGACAATCTGGCCAATGCCCAGGAACTGACCAAGGATGAGCAGGAGGAAGAAGAAGCCT TCAACCAGAAACATGCACTGCAGAAGGCCAAGGAGGTCAGCCCGATGTCTGCTCCCAACATGCCTTCCAT TGAAAGAGACAGAAGGAGAAGACACCACATGTCGATGTGGGAGCCACGCAGCAGCCACCTGAGGGAGCGG AGGCGCCGGCACCACATGTCTGTGTGGGAACAACGCACCAGCCAGCTGAGGAGGCACATGCAAATGTCTA GCCAAGAAGCCCTCAACAAAGAGGAGGCCCCGCCAATGAACCCCCTCAATCCACTCAACCCACTGAGTCC TCTCAACCCCCTCAATGCTCACCCCAGCCTTTATCGGAGGCCCAGGCCCATTGAGGGCCTAGCCCTAGGT CTGGGTCTTGAAAAGTGTGAAGAAGAACGCATCAGTCGTGGGGGCTCCCTCAAGGGGGACATAGGGGGCC TGACTAGCGCCCTGGACAACCAGAGGAGCCCCTTGTCCTTGGGCAAACGGGAGCCACCGTGGCTGCCTAG ATCCTGTCATGGAAACTGTGACCCAATCCAGCAGGAGGCTGGGGGAGGAGAGACTGTGGTGACTTTTGAG GACCGGGCCAGGCACAGGCAGAGTCAGAGGCGAAGCCGGCATCGCCGAGTCAGGACTGAGGGCAAGGACT CTGCCTCTGCATCCCGAAGCAGGTCTGCCAGCCAGGAGCGGAGTCTGGATGAAGGGGTGTCCGTTGAGGG GGAGAAGGAACATGAGCCTCACAGCAGCCACAGGAGCAAAGAGCCGACCATCCACGAAGAGGAGAGAACC CAGGACTTAAGGAGGACCAACAGTCTGATGGTCCCCAGGGGCTCTGGACTGGTAGGGGCTCTTGATGAAG CAGAAACACCTCTAGTCCAGCCCCAACCTGAGCTGGAAGTGGGGAAGGATGCAGCTCTGACAGAGCAGGA GGCTGAAGGCAGCAGTGAACAAGCCCTTCTTGGGGACGTACAGCTGGATGTGGGCCGGGGCATTAGCCAG AGTGAACCGGATCTCTCCTGCATGACAGCCAACATGGACAAGGCTACCACGGAGAGCACCAGTGTCACGG TCGCCATCCCTGATGTGGACCCCTTGGTGGACTCGACAGTGGTGAACATTAGCAACAAGACGGATGGAGA AGCCAGTCCCTTGAAGGAGGCAGAGACTAAAGAGGAAGAAGAAGAGGTAGAGAAGAAGAAGAAGCAGAAG AAGGAGAAGCGAGAGACTGGAAAAGCCATGGTGCCCCATAGCTCTATGTTCATCTTCAGCACCACTAACC CGATCCGCAGGGCCTGCCACTACATTGTGAACCTACGCTACTTTGAAATGTGCATTCTCTTGGTGATTGC GGCCAGCAGCATTGCCCTGGCAGCCGAGGACCCTGTACTAACTAACTCGGAGCGTAACAAAGTCCTGAGA TATTTCGACTATGTTTTCACAGGAGTGTTTACCTTTGAGATGGTTATAAAGATGATAGACCAAGGCTTGA TCCTGCAGGATGGATCATACTTCAGAGACCTCTGGAACATCCTGGACTTTGTAGTAGTGGTTGGTGCATT GGTAGCATTTGCTCTGGCGAACGCTTTGGGAACCAACAAGGGGAGGGATATCAAGACTATCAAGTCTCTG AGGGTACTTCGAGTTCTGCGGCCACTGAAAACCATCAAGCGCTTGCCCAAGCTGAAGGCTGTGTTTGACT GCGTGGTGACCTCCTTGAAGAATGTCTTCAACATACTCATTGTCTACAAGCTCTTCATGTTCATCTTTGC CGTCATCGCTGTTCAGCTATTCAAAGGAAAGTTCTTTTATTGCACTGACAGTTCCAAGGACACAGAGAAG GAGTGCATAGGCAACTATGTAGACCACGAGAAAAACAAGATGGAGGTGAAGGGCCGGGAATGGAAGCGTC ATGAATTCCACTATGACAACATCATCTGGGCCCTGCTTACCCTCTTCACAGTCTCTACAGGGGAAGGATG GCCTCAGGTTCTGCAGCACTCTGTAGATGTGACAGAGGAGGACAGAGGCCCAAGTCGCAGCAACCGCATG GAGATGTCCATCTTTTATGTGGTCTACTTTGTGGTCTTCCCTTTCTTCTTTGTCAATATCTTCGTGGCTC TCATTATCATCACCTTCCAGGAGCAAGGGGACAAGATGATGGAGGAGTGTAGCCTGGAGAAGAATGAGCG GGCATGCATCGACTTCGCCATTAGTGCCAAACCTCTCACCCGCTACATGCCACAGAACAGGCACACCTTC CAGTACCGTGTGTGGCACTTTGTAGTCTCCCCATCCTTTGAGTATACCATCATGGCCATGATTGCCTTGA ACACTGTCGTGCTGATGATGAAGTACTACTCTGCTCCTTGCACCTATGAGCTCGCCTTAAAGTACCTGAA CATCGCCTTCACTATGGTGTTTTCCCTGGAGTGTGTCCTAAAGGTCATTGCTTTCGGCTTCCTGAACTAT TTCCGAGATACCTGGAACATCTTTGACTTCATCACGGTGATCGGCAGTATCACAGAAATTATCCTGACAG ATAGCAAGCTGGTGAACACCAGTGGCTTCAATATGAGCTTTCTGAAGCTCTTCCGAGCTGCACGGCTCAT AAAGCTTCTGAGACAGGGCTACACCATCCGCATCTTACTGTGGACCTTCGTGCAGTCCTTTAAGGCTCTT CCCTACGTCTGCCTTCTCATTGCCATGCTTTTCTTCATCTACGCCATCATTGGGATGCAGGTGTTTGGAA ACATAAAGTTAGATGAGGAGAGTCACATCAACCGGCACAACAACTTCCGGAGTTTCTTTGGATCCCTCAT GCTGCTTTTCAGGAGTGCCACGGGGGAGGCTTGGCAGGAGATTATGCTGTCCTGTCTCGGTGAGAAGGGT TGTGAGCCTGACACCACTGCACCCTCAGGGCAGAATGAGAGTGAGCGCTGTGGCACTGACCTGGCCTACG TTTATTTTGTCTCCTTCATCTTCTTCTGCTCCTTCCTGATGCTCAACCTGTTCGTGGCTGTCATCATGGA CAACTTTGAGTACCTGACTCGAGATTCCTCCATCCTGGGGCCTCACCACTTAGATGAGTTTGTCCGTGTC TGGGCAGAATATGACAGAGCAGCATGTGGCCGCATCCATTACACTGAGATGTATGAAATGCTGACTCTCA TGTCACCACCGCTAGGCCTCGGCAAGAGATGTCCCTCCAAAGTGGCCTATAAGAGGCTGGTCCTGATGAA CATGCCAGTGGCTGAGGACATGACAGTCCACTTCACCTCCACACTTATGGCTCTGATCCGGACAGCTCTG GACATTAAAATCGCCAAAGGTGGTGCAGACAGACAGCAGCTAGACTCGGAGCTGCAAAAAGAGACCCTGG CCATTTGGCCTCACCTGTCCCAAAAGATGTTGGATCTGCTTGTGCCCATGCCCAAAGCCTCTGACCTGAC TGTGGGCAAGATCTATGCAGCAATGATGATCATGGACTACTATAAACAGAGTAAGGTAAAGAAACAGAGA CAGCAGCTGGAAGAACAGAAAAATGCACCCATGTTCCAGCGCATGGAGCCCTCATCACTGCCTCAGGAGA TCATTGCTAATGCCAAAGCCCTGCCTTATCTCCAGCAGGACCCTGTTTCTGGCCTGAGTGGTCGGAGTGG ATACCCTTCAATGAGTCCACTCTCCCCTCAAGAAATATTCCAGTTGGCTTGTATGGACCCAGCGGATGAT GGACAATTCCAGGAACAACAATCTCTGGTGGTAACAGACCCTAGCTCTATGCGACGTTCATTTTCTACAA TTCGGGATAAGCGTTCAAATTCCTCCTGGTTGGAGGAGTTCTCCATGGAACGCAGCAGTGAAAACACTTA CAAGTCGCGGCGCAGAAGTTACCATTCCTCCCTGAGGCTGTCAGCACACCGCCTGAATTCTGACTCTGGT CACAAGTCTGACACTCACCGATCAGGAGGCAGGGAGAGAGGCCGATCCAAAGAACGAAAGCACCTCCTCT CTCCAGATGTCTCCCGCTGCAATTCTGAAGAGCGAGGGACCCAGGCCGACTGGGAGTCCCCAGAACGTCG CCAGTCCAGGTCACCCAGTGAAGGAAGGTCACAGACCCCCAATAGACAGGGTACCGGTTCCCTAAGTGAG AGCTCCATCCCCTCTATTTCCGACACCAGCACCCCAAGACGAAGCCGTCGCCAGCTCCCACCTGTGCCAC CAAAGCCTCGTCCCCTCCTCTCCTACAGCTCCCTGATGAGACACACTGGCGGCATCTCTCCACCTCCTGA TGGAAGTGAGGGTGGATCCCCACTGGCCTCTCAAGCTCTGGAGAGCAACAGCGCTTGCCTGACCGAGTCT TCCAACTCCTTGCACCCCCAGCAGGGCCAGCATCCTTCCCCACAGCACTACATCTCCGAGCCCTATCTGG CCCTCCATGAAGACTCCCACGCCTCAGACTGTGGCGAGGAGGAGACACTCACCTTTGAGGCAGCCGTAGC CACTAGCTTGGGCCGGTCCAATACCATTGGCTCTGCGCCACCCCTACGGCACAGCTGGCAGATGCCTAAT GGGCACTATCGGCGGCGGAGGCGTGGGGGGCCTGGGCCTGGCATGATGTGTGGAGCTGTCAGTGACCTCC TGAGTGACACGGAAGAAGACGATAAGTGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR223012 representing NM_009782 Red=Cloning site Green=Tags(s) MARFGEAVVVGRPGSGDGDSDQSRNRQGTPVPASGPAAAYKQSKAQRARTMALYNPIPVRQNCFTVNRSL FIFGEDNIVRKYAKKLIDWPPFEYMILATIIANCIVLALEQHLPEDDKTPMSRRLEKTEPYFIGIFCFEA GIKIVALGFIFHKGSYLRNGWNVMDFIVVLSGILATAGTHFNTHVDLRTLRAVRVLRPLKLVSGIPSLQI VLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYSGKLHRACFMNNSGILEGFDPPHPCGVQGCPAGYEC KDWIGPNDGITQFDNILFAVLTVFQCITMEGWTTVLYNTNDALGATWNWLYFIPLIIIGSFFVLNLVLGV LSGEFAKERERVENRRAFMKLRRQQQIERELNGYRAWIDKAEEVMLAEENKNSGTSALEVLRRATIKRSR TEAMTRDSSDEHCVDISSVGTPLARASIKSTKVDGASYFRHKERLLRISIRHMVKSQVFYWIVLSVVALN TACVAIVHHNQPQWLTHLLYYAEFLFLGLFLLEMSLKMYGMGPRLYFHSSFNCFDFGVTVGSIFEVVWAI FRPGTSFGISVLRALRLLRIFKITKYWASLRNLVVSLMSSMKSIISLLFLLFLFIVVFALLGMQLFGGRF NFNDGTPSANFDTFPAAIMTVFQILTGEDWNEVMYNGIRSQGGVSSGMWSAIYFIVLTLFGNYTLLNVFL AIAVDNLANAQELTKDEQEEEEAFNQKHALQKAKEVSPMSAPNMPSIERDRRRRHHMSMWEPRSSHLRER RRRHHMSVWEQRTSQLRRHMQMSSQEALNKEEAPPMNPLNPLNPLSPLNPLNAHPSLYRRPRPIEGLALG LGLEKCEEERISRGGSLKGDIGGLTSALDNQRSPLSLGKREPPWLPRSCHGNCDPIQQEAGGGETVVTFE DRARHRQSQRRSRHRRVRTEGKDSASASRSRSASQERSLDEGVSVEGEKEHEPHSSHRSKEPTIHEEERT QDLRRTNSLMVPRGSGLVGALDEAETPLVQPQPELEVGKDAALTEQEAEGSSEQALLGDVQLDVGRGISQ SEPDLSCMTANMDKATTESTSVTVAIPDVDPLVDSTVVNISNKTDGEASPLKEAETKEEEEEVEKKKKQK KEKRETGKAMVPHSSMFIFSTTNPIRRACHYIVNLRYFEMCILLVIAASSIALAAEDPVLTNSERNKVLR YFDYVFTGVFTFEMVIKMIDQGLILQDGSYFRDLWNILDFVVVVGALVAFALANALGTNKGRDIKTIKSL RVLRVLRPLKTIKRLPKLKAVFDCVVTSLKNVFNILIVYKLFMFIFAVIAVQLFKGKFFYCTDSSKDTEK ECIGNYVDHEKNKMEVKGREWKRHEFHYDNIIWALLTLFTVSTGEGWPQVLQHSVDVTEEDRGPSRSNRM EMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEECSLEKNERACIDFAISAKPLTRYMPQNRHTF QYRVWHFVVSPSFEYTIMAMIALNTVVLMMKYYSAPCTYELALKYLNIAFTMVFSLECVLKVIAFGFLNY FRDTWNIFDFITVIGSITEIILTDSKLVNTSGFNMSFLKLFRAARLIKLLRQGYTIRILLWTFVQSFKAL PYVCLLIAMLFFIYAIIGMQVFGNIKLDEESHINRHNNFRSFFGSLMLLFRSATGEAWQEIMLSCLGEKG CEPDTTAPSGQNESERCGTDLAYVYFVSFIFFCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRV WAEYDRAACGRIHYTEMYEMLTLMSPPLGLGKRCPSKVAYKRLVLMNMPVAEDMTVHFTSTLMALIRTAL DIKIAKGGADRQQLDSELQKETLAIWPHLSQKMLDLLVPMPKASDLTVGKIYAAMMIMDYYKQSKVKKQR QQLEEQKNAPMFQRMEPSSLPQEIIANAKALPYLQQDPVSGLSGRSGYPSMSPLSPQEIFQLACMDPADD GQFQEQQSLVVTDPSSMRRSFSTIRDKRSNSSWLEEFSMERSSENTYKSRRRSYHSSLRLSAHRLNSDSG HKSDTHRSGGRERGRSKERKHLLSPDVSRCNSEERGTQADWESPERRQSRSPSEGRSQTPNRQGTGSLSE SSIPSISDTSTPRRSRRQLPPVPPKPRPLLSYSSLMRHTGGISPPPDGSEGGSPLASQALESNSACLTES SNSLHPQQGQHPSPQHYISEPYLALHEDSHASDCGEEETLTFEAAVATSLGRSNTIGSAPPLRHSWQMPN GHYRRRRRGGPGPGMMCGAVSDLLSDTEEDDKC TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_009782
ORF Size	6819 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_009782.3, NP_033912.2
RefSeq Size	12697 bp
RefSeq ORF	6822 bp
Locus ID	12290
MW	257.7 kDa
Gene Summary	This gene encodes an integral membrane protein that belongs to the calcium channel alpha-1 subunits family. Voltage-sensitive calcium channels mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes. Voltage-dependent calcium channels are multi-subunit complexes, comprised of alpha-1, alpha-2, beta and delta subunits in a 1:1:1:1 ratio. The isoform alpha-1E gives rise to R-type calcium currents and belongs to the high-voltage activated group. Calcium channels containing the alpha-1E subunit may be involved in the modulation of neuronal firing patterns, an important component of information processing. [provided by RefSeq, Jul 2008]

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