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Phc1 (NM_001042623) Mouse Tagged ORF Clone
CAT＃: MR223712
- TrueORF®
Phc1 (Myc-DDK-tagged) - Mouse polyhomeotic-like 1 (Drosophila) (Phc1), transcript variant 2
ORF Plasmid: tGFP
"NM_001042623" in other vectors (3)
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CNY 9,600.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AW557034; Edr; Edr1; Mph1; Rae-28; rae28 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR223712 representing NM_001042623
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAACGGAGAGTGAGCAGAACTCTAGTTCGACCAATGGGAGTTCCAGTTCAGGGGCCAGCTCTCGGC CCCAGATAGCACAGATGTCCCTGTATGAACGGCAAGCAGTGCAGGCTCTGCAAGCACTGCAGCGGCAACC TAATGCGGCTCAATATTTCCACCAGTTCATGCTCCAGCAACAGCTCAGCAATGCCCAACTGCACAGCCTG GCAGCTGTCCAGCAGGCCACGATTGCTGCCAGTCGACAGGCTAGCTCCCCAAACAGCAGCACTGCACAGC AGCAGACCGCTACCACCCAGGCCTCGATGAATCTCGCGACCACGTCAGCTGCTCAGCTCATCAGCCGATC GCAGAGTGTGAGCTCCCCCAGTGCTACCACCCTGACGCAGTCGGTGCTGCTGGGGAACACCACTTCCCCA CCTCTCAACCAGTCCCAGGCCCAGATGTATCTGCGGGTGCAGAACTTGGCAGTGAGGAACCAACAGGCTT CAGCTCAAGGACCCCAAATGCCAGGCTCCACTCAGAAGGCCATCCCTCCTGGAGCTTCTCCTGTTTCTGG CCTCTCCCAGACTTCTAGCCAGGCCCTAGCCGTGGCACAGGCTTCTTCTGGGGCCTCAGGCCAGTCACTC AACCTTAGTCAAGCCGGTGGAGGCAGTGGGAATAGCCTCCCAGGCTCCATGGGTCCAGGTGGAGGTGGCC AGGCGCCTGGGGGTTTAGGTCAGTTGCCTTCTTCAGGATTGACTGGTGGAAGCTGTCCCCGGAAGGGCAC AGGAGTGGTGCAGCCCTTACCTGCAGCTCAAACAGTGACTGTGAGCCAAGGAAGCCAAACAGAAGCAGAA AGTGCAGCAGCCAAGAAGGCAGAAGCAGATGGGAGTGGCCAGCAGAGTGTGGGCATGAACCTGACTCGGA CAGCCACACCTGCCCCCAGCCAGACGCTTATTAGCTCAGCCACATACACACAGATCCAGCCCCATTCACT GATTCAGCAACAGCAGCAGATCCACCTCCAGCAGAAGCAAGTGGTGATCCAGCAGCAGATTGCCATCCAC CACCAGCAACAGTTCCAACACCGCCAGTCCCAGCTGCTTCACACAGCCACACACCTCCAGTTGGCCCAGC AGCAGCAGCAGCAACAGCAGCAACAGCAGCAACAACAGCAACAGCAGCAGCAGCAGCAGCAAGGAACAAC CCTCACTGCCCCTCAGCCACCCCAGGTCCCACCTACTCAGCAGGTCCCACCTTCCCAATCACAACAGCAA GCCCAGACTCTGGTGGTCCAACCCATGCTTCAGTCTTCACCCCTGACTCTTCCACCTGAACCAACCTCTA AACCACCCATCCCCATTCAGTCCAAACCTCCTGTAGCGCCTATCAAACCTCCTCAACTAGGAGCTGCTAA GATGTCAGCTACACAGCAGCCACCACCACATATCCCCGTGCAAGTTGTCGGTACCCGGCAGCCAGGTTCA GCCCAAGCACAGGCTTTGGGATTAGCACAGCTGGCTGCTGCTGTACCTACTCCCCGTGGAATAACAGGAG CAGTCCAGCCTGGCCAGGCTCATCTGGCCTCCTCGCCACCTTCATCACAGGCGGCTCCTGGTGCACTTCA AGAATGTCCTCCTGCATTGGCTGCTGGGATGACCCTTGCTCCTGTGCAGGGGACAGCACATGTGGTAAAG GGTGGGCCTACAGCCTCCTCTCCTGTTGTGGCCCAGGTCCCTGCTGCTTTCTACATGCAGTCGGTGCACC TGCCGGGTAAAGCCCAGACCTTGGCTGTGAAACGCAAAGCTGAGTCTGAGGAGGAGAGAGATGACCTCTC CGCATTGGCCTCTGTGCTTCCTACAAAGGCCTCCCCAGCAGCAGAGAGCCCAAAGGTAATAGAGGAGAAG AATAGTCTTGGAGAGAAAGCTGAACCAGTGGCCAGTTTGAATGCTAATCCTCCAAACAGTGATCTAGTAG CCTTGGCCCCTACCCCATCAGCACCACCTCCTACTCTGGCCTTGGTGTCCAGACAGATGGGTGACTCAAA ACCCCCACAGGCCATTGTGAAGCCCCAGATTCTCACGCACATCATTGAAGGCTTTGTTATCCAGGAAGGA GCAGAGCCTTTTCCGGTGGGATGTTCTCAGTTCCTGAAAGAAACCAAGAAGCCACTGCAGGCTGGCCTTC CGACCGGGCTGAATGAGAGTCAGCCAAGTGGCCCTTTGGGGGGAGACAGCCCCTCTGTAGAGTTAGAGAA GAAGGCAAATCTCCTCAAGTGTGAGTACTGTGGCAAGTACGCCCCAGCAGAGCAGTTTCGAGGCTCTAAG AGGTTCTGCTCCATGACGTGTGCAAAGAGGTACAATGTGAGCTGCAGCCACCAGTTCCGCTTGAAGAGGA AAAAAATGAAAGAGTTTCAGGAAGCCAGCTATGCACGTGTTCGGAGGCGTGGACCCCGCCGCAGCTCCTC TGATATTGCTCGTGCTAAGATCCAGGGCAAGCGCCATCGGGGTCAAGAGGACTCTAGCCGGGGATCAGAT AATTCCAGCTATGATGAAGCACTCTCTCCAACATCTCCTGGGCCACTGTCAGTAAGAGCCGGGCACGGAG AACGTGATCTAGGAAACACCATTACAACCCCATCCACGCCAGAGTTACAGGGCATCAATCCCGTGTTCTT GTCCAGTAATCCTAGCCAATGGAGCGTCGAGGAGGTCTACGAGTTTATTGCTTCTCTGCAAGGCTGCCAA GAGATTGCAGAAGAGTTTCGTTCCCAGGAGATTGATGGACAGGCCCTTTTATTACTTAAAGAAGAACATC TGATGAGTGCCATGAACATCAAATTAGGTCCTGCTCTCAAGATCTGTGCCAAGATCAATGTCCTCAAGGA GACC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR223712 representing NM_001042623
Red=Cloning site Green=Tags(s) METESEQNSSSTNGSSSSGASSRPQIAQMSLYERQAVQALQALQRQPNAAQYFHQFMLQQQLSNAQLHSL AAVQQATIAASRQASSPNSSTAQQQTATTQASMNLATTSAAQLISRSQSVSSPSATTLTQSVLLGNTTSP PLNQSQAQMYLRVQNLAVRNQQASAQGPQMPGSTQKAIPPGASPVSGLSQTSSQALAVAQASSGASGQSL NLSQAGGGSGNSLPGSMGPGGGGQAPGGLGQLPSSGLTGGSCPRKGTGVVQPLPAAQTVTVSQGSQTEAE SAAAKKAEADGSGQQSVGMNLTRTATPAPSQTLISSATYTQIQPHSLIQQQQQIHLQQKQVVIQQQIAIH HQQQFQHRQSQLLHTATHLQLAQQQQQQQQQQQQQQQQQQQQQQGTTLTAPQPPQVPPTQQVPPSQSQQQ AQTLVVQPMLQSSPLTLPPEPTSKPPIPIQSKPPVAPIKPPQLGAAKMSATQQPPPHIPVQVVGTRQPGS AQAQALGLAQLAAAVPTPRGITGAVQPGQAHLASSPPSSQAAPGALQECPPALAAGMTLAPVQGTAHVVK GGPTASSPVVAQVPAAFYMQSVHLPGKAQTLAVKRKAESEEERDDLSALASVLPTKASPAAESPKVIEEK NSLGEKAEPVASLNANPPNSDLVALAPTPSAPPPTLALVSRQMGDSKPPQAIVKPQILTHIIEGFVIQEG AEPFPVGCSQFLKETKKPLQAGLPTGLNESQPSGPLGGDSPSVELEKKANLLKCEYCGKYAPAEQFRGSK RFCSMTCAKRYNVSCSHQFRLKRKKMKEFQEASYARVRRRGPRRSSSDIARAKIQGKRHRGQEDSSRGSD NSSYDEALSPTSPGPLSVRAGHGERDLGNTITTPSTPELQGINPVFLSSNPSQWSVEEVYEFIASLQGCQ EIAEEFRSQEIDGQALLLLKEEHLMSAMNIKLGPALKICAKINVLKET TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001042623 |
| ORF Size | 2874 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001042623.2, NP_001036088.1 |
| RefSeq Size | 3743 bp |
| RefSeq ORF | 2877 bp |
| Locus ID | 13619 |
| MW | 101.1 kDa |
| Gene Summary | Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. Required for proper control of cellular levels of GMNN expression (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG223712 | Phc1 (tGFP-tagged) - Mouse polyhomeotic-like 1 (Drosophila) (Phc1) transcript variant 2, (10ug) |
CNY 10,550.00 |
|
| MR223712L3 | Lenti ORF clone of Phc1 (Myc-DDK-tagged) - Mouse polyhomeotic-like 1 (Drosophila) (Phc1), transcript variant 2 |
CNY 11,500.00 |
|
| MR223712L4 | Lenti ORF clone of Phc1 (mGFP-tagged) - Mouse polyhomeotic-like 1 (Drosophila) (Phc1), transcript variant 2 |
CNY 11,500.00 |
