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Ube2l3 (NM_009456) Mouse Tagged ORF Clone
CAT＃: MR223806
- TrueORF®
Ube2l3 (Myc-DDK-tagged) - Mouse ubiquitin-conjugating enzyme E2L 3 (Ube2l3)
ORF Plasmid: tGFP
"NM_009456" in other vectors (4)
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CNY 1,200.00
CNY 3,705.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | C79827; Ubce7; UbcM4 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR223806 representing NM_009456
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCCAGCAGGAGGCTGATGAAGGAGCTTGAAGAGATCCGCAAATGTGGAATGAAAAACTTCCGTA ACATCCAGGTTGATGAAGCTAATTTATTGACTTGGCAAGGGCTTATTGTTCCTGACAACCCTCCATATGA TAAGGGAGCCTTCAGAATTGAAATCAACTTTCCAGCAGAGTATCCATTCAAACCACCCAAGATCACATTT AAAACAAAGATCTACCACCCTAACATCGATGAGAAGGGGCAGGTCTGTCTGCCAGTCATTAGTGCTGAAA ACTGGAAGCCAGCCACCAAGACTGACCAAGTAATCCAGTCCCTCATAGCACTGGTGAATGACCCCCAGCC TGAGCACCCACTCCGGGCTGACCTAGCTGAAGAATACTCTAAGGACCGTAAAAAATTCTGTAAGAATGCT GAAGAGTTTACAAAGAAATATGGGGAAAAGCGACCTGTGGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR223806 representing NM_009456
Red=Cloning site Green=Tags(s) MAASRRLMKELEEIRKCGMKNFRNIQVDEANLLTWQGLIVPDNPPYDKGAFRIEINFPAEYPFKPPKITF KTKIYHPNIDEKGQVCLPVISAENWKPATKTDQVIQSLIALVNDPQPEHPLRADLAEEYSKDRKKFCKNA EEFTKKYGEKRPVD TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_009456 |
| ORF Size | 462 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_009456.2, NP_033482.1 |
| RefSeq Size | 2609 bp |
| RefSeq ORF | 465 bp |
| Locus ID | 22195 |
| UniProt ID | P68037 |
| MW | 18.3 kDa |
| Gene Summary | Ubiquitin-conjugating enzyme E2 that specifically acts with HECT-type and RBR family E3 ubiquitin-protein ligases. Does not function with most RING-containing E3 ubiquitin-protein ligases because it lacks intrinsic E3-independent reactivity with lysine: in contrast, it has activity with the RBR family E3 enzymes, such as PRKN and ARIH1, that function like function like RING-HECT hybrids. Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'-linked polyubiquitination. Involved in the selective degradation of short-lived and abnormal proteins. Down-regulated during the S-phase it is involved in progression through the cell cycle. Regulates nuclear hormone receptors transcriptional activity. May play a role in myelopoiesis.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC209609 | Ube2l3 (untagged) - Mouse ubiquitin-conjugating enzyme E2L 3 (Ube2l3), (10ug) |
CNY 3,990.00 |
|
| MG223806 | Ube2l3 (tGFP-tagged) - Mouse ubiquitin-conjugating enzyme E2L 3 (Ube2l3), (10ug) |
CNY 2,850.00 |
|
| MR223806L3 | Lenti ORF clone of Ube2l3 (Myc-DDK-tagged) - Mouse ubiquitin-conjugating enzyme E2L 3 (Ube2l3) |
CNY 4,750.00 |
|
| MR223806L4 | Lenti ORF clone of Ube2l3 (mGFP-tagged) - Mouse ubiquitin-conjugating enzyme E2L 3 (Ube2l3) |
CNY 4,750.00 |
