Sox7 (NM_011446) Mouse Tagged ORF Clone

CAT#: MR223853

  • TrueORF®

Sox7 (Myc-DDK-tagged) - Mouse SRY-box containing gene 7 (Sox7)

ORF Plasmid: DDK tGFP



  "NM_011446" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 2,850.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR223853 representing NM_011446
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCTCGCTGCTGGGCGCCTATCCGTGGACCGAGGGACTGGAGTGTCCCGCCCTGGAAGCCGAGCTGT
CGGATGGGCTGTCGCCGCCCGCCGTCCCCCGACCTTCAGGGGACAAGAGTTCGGAAAGCCGGATCCGGCG
GCCCATGAATGCCTTCATGGTGTGGGCCAAGGATGAGAGGAAACGTCTGGCAGTGCAGAACCCGGACCTG
CACAACGCGGAGCTCAGCAAGATGCTGGGAAAGTCATGGAAGGCGCTGACACTGTCCCAGAAGAGACCCT
ATGTGGATGAGGCAGAGCGGCTGCGCCTGCAGCACATGCAGGATTACCCCAACTACAAGTACCGGCCCCG
CAGGAAGAAACAAGGCAAGCGCCTCTGCAAGCGCGTGGACCCTGGCTTCCTCCTCAGCTCCCTCTCTCGT
GACCAGAACACGCTGCCTGAGAAAAACGGCATTGGCAGGGGGGAGAAGGAGGACAGGGGTGAGTACTCCC
CAGGGGCCACCTTGCCTGGACTGCACAGCTGCTACCGCGAAGGTGCAGCTGCTGCCCCTGGCAGTGTGGA
CACGTATCCCTACGGGCTGCCCACACCTCCGGAGATGTCGCCCCTGGATGCGCTGGAGCCAGAGCAGACC
TTCTTCTCGTCCTCATGTCAGGAGGAGCATGGTCACCCCCATCACCTCCCCCATCTACCAGGGCCCCCTT
ACTCACCGGAGTTCACACCTAGTCCCCTCCACTGCAGCCACCCTCTAGGTTCTTTAGCCCTTGGCCAATC
CCCAGGGGTTTCTATGATGTCCTCTGTTTCTGGATGTCCCCCATCTCCAGCCTATTACTCCCATGCCACC
TACCACCCTCTCCACCCCAACCTCCAGGCCCACCTGGGCCAGCTGTCCCCACCTCCGGAGCACCCTGGCT
TTGACACCTTGGATCAGCTAAGCCAGGTGGAACTTCTGGGAGACATGGATCGCAATGAATTTGATCAGTA
TTTGAACACTCCCGGCCACCCTGACTCTGCTGCAGGGGTTGGAACCCTCACTGGGCATGTCCCGCTCTCC
CAGGGGACTCCCACAGGCCCTACAGAGACCAGCCTCATCTCAGTCCTGGCTGATGCCACGGCCACGTATT
ACAACAGCTACAGTGTGTCA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR223853 representing NM_011446
Red=Cloning site Green=Tags(s)

MASLLGAYPWTEGLECPALEAELSDGLSPPAVPRPSGDKSSESRIRRPMNAFMVWAKDERKRLAVQNPDL
HNAELSKMLGKSWKALTLSQKRPYVDEAERLRLQHMQDYPNYKYRPRRKKQGKRLCKRVDPGFLLSSLSR
DQNTLPEKNGIGRGEKEDRGEYSPGATLPGLHSCYREGAAAAPGSVDTYPYGLPTPPEMSPLDALEPEQT
FFSSSCQEEHGHPHHLPHLPGPPYSPEFTPSPLHCSHPLGSLALGQSPGVSMMSSVSGCPPSPAYYSHAT
YHPLHPNLQAHLGQLSPPPEHPGFDTLDQLSQVELLGDMDRNEFDQYLNTPGHPDSAAGVGTLTGHVPLS
QGTPTGPTETSLISVLADATATYYNSYSVS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_011446
ORF Size 1140 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_011446.1, NP_035576.1
RefSeq Size 3266 bp
RefSeq ORF 1143 bp
Locus ID 20680
UniProt ID P40646
MW 41.9 kDa
Gene Summary Binds to and activates the CDH5 promoter, hence plays a role in the transcriptional regulation of genes expressed in the hemogenic endothelium and blocks further differentiation into blood precursors. May be required for the survival of both hematopoietic and endothelial precursors during specification. May play a role in skeletal myogenesis and up-regulate the expression of muscle markers, such as PAX3/PAX7 and Meox1. Competes with GATA4 for binding and activation of the FGF3 promoter. Represses Wnt/beta-catenin-stimulated transcription. Probably acts by targeting CTNNB1 to proteasomal degradation. Binds the DNA sequence 5'-AACAAT-3'.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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