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Robo1 (NM_019413) Mouse Tagged ORF Clone
CAT＃: MR224013
- TrueORF®
Robo1 (Myc-DDK-tagged) - Mouse roundabout homolog 1 (Drosophila) (Robo1)
ORF Plasmid: tGFP
"NM_019413" in other vectors (4)
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CNY 11,032.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AW494633; AW742721; DUTT1; Gm310 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR224013 representing NM_019413
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATCGCGGAGCCTGCTCGCTTTTACCTCTTTGGATTAATATGTCTCTGTTCAGGCTCCCGTCTTCGTC AGGAAGATTTTCCACCCCGCATTGTTGAACACCCTTCAGACCTGATCGTCTCCAAAGGAGAACCTGCCAC TCTGAACTGTAAAGCCGAAGGCCGCCCCACCCCGACGATAGAATGGTACAAAGGTGGGGAAAGAGTGGAG ACGGACAAAGATGACCCGCGCTCACACCGGATGCTGCTGCCAAGCGGGTCTTTATTTTTCTTACGCATAG TCCATGGACGGAAGAGTAGGCCCGACGAAGGGGTCTACATCTGTGTGGCTAGGAATTACCTCGGAGAGGC TGTGAGCCACAACGCATCGCTGGAGGTAGCTATACTACGGGATGACTTCAGACAAAACCCTTCAGATGTC ATGGTGGCAGTAGGCGAACCTGCAGTCATGGAATGCCAGCCTCCACGGGGCCATCCAGAGCCCACCATCT CCTGGAAAAAGGATGGCTCACCCCTGGATGATAAAGATGAAAGAATCACTATACGAGGAGGAAAACTCAT GATCACTTATACGCGTAAAAGCGATGCTGGCAAGTATGTTTGCGTTGGCACCAACATGGTGGGGGAACGG GAGAGTGAAGTCGCAGAGCTAACCGTTTTAGAGAGACCATCGTTTGTGAAGAGACCCAGTAATTTGGCAG TGACTGTGGATGACAGTGCAGAATTTAAATGTGAGGCCCGAGGAGACCCTGTGCCTACAGTTCGATGGAG GAAAGATGACGGAGAGCTGCCCAAATCCAGATATGAAATCCGAGATGATCATACTTTGAAAATTCGGAAG GTGACTGCTGGCGACATGGGATCATACACTTGTGTGGCAGAAAATATGGTCGGGAAAGCTGAAGCTTCCG CTACTTTGACAGTTCAAGAGCCGCCACATTTCGTTGTAAAACCTCGGGACCAGGTGGTTGCCTTGGGAAG AACTGTGACGTTTCAGTGCGAAGCAACTGGAAATCCTCAACCAGCTATCTTTTGGAGGAGAGAGGGGAGT CAGAATCTACTTTTCTCCTACCAGCCTCCACAGTCCTCTAGCCGATTTTCAGTTTCGCAGACAGGAGACC TCACCATTACGAACGTCCAGCGGTCTGATGTCGGCTACTACATCTGTCAGACTTTAAATGTTGCTGGAAG TATCATCACAAAGGCCTATTTGGAAGTTACTGATGTGATTGCAGACCGGCCTCCCCCAGTCATTCGGCAA GGTCCTGTGAATCAGACTGTAGCAGTGGATGGCACTTTAATCCTCAGCTGTGTGGCCACAGGCAGTCCAG CACCCACAATTCTGTGGAGGAAGGATGGAGTCCTTGTTTCAACCCAAGATTCTCGGATCAAACAGCTGGA GAGTGGCGTGCTGCAGATCCGCTACGCTAAGCTGGGTGACACGGGTCGGTACACCTGCACCGCATCGACC CCCAGCGGTGAGGCCACGTGGAGCGCTTACATCGAAGTCCAGGAATTTGGGGTTCCAGTTCAACCCCCAA GACCCACCGACCCCAACTTAATCCCCAGCGCCCCCTCAAAGCCCGAAGTTACAGATGTCAGCAAAAACAC GGTCACTTTATCGTGGCAGCCAAACTTGAACTCAGGAGCAACCCCAACATCTTATATTATAGAAGCCTTC AGCCATGCATCTGGTAGCAGCTGGCAGACGGCGGCAGAAAATGTGAAAACAGAAACATTTGCCATCAAAG GACTCAAACCTAACGCCATTTACCTTTTCCTGGTGAGGGCAGCAAATGCCTATGGAATTAGTGATCCAAG CCAAATATCAGATCCAGTGAAAACGCAAGATGTCCCACCAACCAGCCAAGGGGTGGACCACAAGCAGGTC CAAAGAGAACTGGGGAATGTTGTCTTACATCTGCACAACCCGACCATCCTTTCTTCCTCCTCAGTCGAAG TGCACTGGACGGTGGACCAACAATCTCAATATATTCAAGGCTATAAAATTCTCTACCGCCCATCTGGAGC CAGCCATGGTGAATCGGAGTGGTTAGTTTTTGAAGTGAGGACGCCAACGAAAAACAGCGTGGTGATCCCC GATCTCAGAAAAGGCGTCAACTACGAGATTAAGGCTCGCCCATTTTTTAATGAGTTTCAAGGAGCAGACA GTGAAATCAAATTTGCCAAAACCTTAGAAGAAGCACCAAGTGCCCCTCCCCGAAGTGTAACTGTATCCAA GAATGATGGAAATGGGACTGCAATCCTGGTCACTTGGCAACCGCCTCCTGAAGACACACAAAATGGAATG GTCCAAGAATATAAGGTTTGGTGTCTCGGTAATGAAACGAAGTATCACATAAACAAGACAGTCGATGGCT CGACCTTCTCGGTGGTCATCCCCTCTCTGGTTCCTGGGATCCGATACAGTGTGGAGGTGGCAGCGAGCAC TGGGGCGGGTCCCGGGGTGAAGAGCGAACCTCAGTTCATCCAGTTAGATTCTCACGGAAACCCCGTGTCT CCCGAGGACCAAGTCAGCCTTGCTCAGCAGATCTCCGACGTGGTGAGGCAGCCGGCGTTCATTGCAGGCA TTGGGGCAGCCTGTTGGATTATCCTCATGGTCTTCAGCATCTGGCTTTACAGACACCGCAAGAAGAGAAA CGGACTCACCAGCACGTATGCGGGTATCCGGAAAGTCCCGTCCTTTACCTTCACGCCAACAGTAACTTAT CAAAGAGGAGGCGAAGCCGTCAGCAGTGGAGGGAGGCCGGGACTCCTCAACATTAGTGAACCTGCCACGC AGCCATGGCTGGCAGACACGTGGCCCAACACAGGCAACAACCACAATGACTGCTCCATCAACTGCTGCAC GGCCGGCAATGGCAACAGCGACAGCAACCTGACAACCTACAGTCGCCCAGCTGATTGTATAGCCAATTAT AACAACCAACTGGATAACAAACAAACAAATCTGATGCTCCCTGAGTCAACTGTTTATGGTGATGTGGACC TTAGTAACAAAATCAATGAGATGAAAACCTTCAATAGCCCAAATCTGAAGGACGGGCGTTTTGTCAATCC ATCAGGGCAGCCCACTCCCTATGCCACCACTCAACTCATCCAGGCGAACCTCAGCAACAATATGAATAAT GGCGCCGGGGACTCCAGCGAGAAGCACTGGAAGCCTCCGGGACAGCAGAAACCGGAAGTGGCACCAATTC AGTATAACATCATGGAGCAAAACAAGCTGAACAAAGATTATCGGGCAAATGACACGATCCCTCCAACCAT CCCATACAACCAGTCCTATGACCAGAACACAGGAGGGTCTTATAACAGCTCAGATCGGGGCAGTAGTACA TCCGGGAGTCAAGGCCACAAGAAAGGGGCACGGACACCAAAGGCACCCAAGCAGGGTGGCATGAACTGGG CAGACTTGCTGCCCCCTCCTCCGGCGCATCCTCCCCCACATAGCAACAGTGAAGAATACAACATGTCCGT AGATGAAAGCTATGATCAAGAAATGCCCTGTCCAGTGCCACCTGCGCGGATGTATTTGCAACAGGATGAA TTAGAGGAAGAGGAAGATGAAAGAGGCCCCACTCCCCCTGTCCGGGGGGCAGCTTCTTCTCCAGCTGCTG TGTCCTACAGCCACCAATCCACGGCCACTCTCACCCCTTCTCCCCAGGAAGAGCTCCAGCCCATGTTACA GGATTGTCCGGAGGACCTCGGGCACATGCCCCACCCACCAGACAGGAGGCGGCAGCCCGTGAGTCCTCCT CCACCGCCACGGCCAATTTCCCCACCTCATACTTACGGCTACATTTCAGGACCCCTTGTCTCAGATATGG ATACCGATGCCCCAGAAGAGGAGGAGGACGAAGCTGACATGGAAGTTGCCAAAATGCAGACCCGAAGGCT GTTGTTACGTGGGCTGGAGCAGACCCCAGCATCCAGTGTTGGGGACCTTGAGAGCTCCGTCACTGGGTCC ATGATCAATGGCTGGGGCTCAGCCTCGGAAGAGGACAACATTTCCAGTGGGCGCTCCAGTGTCAGTTCAT CCGATGGCTCCTTTTTCACTGACGCTGATTTTGCCCAGGCAGTTGCTGCAGCTGCGGAGTATGCGGGCCT GAAAGTGGCTCGCCGCCAAATGCAAGATGCTGCTGGCCGCCGCCACTTCCATGCCTCTCAGTGCCCAAGG CCCACGAGTCCTGTGTCCACAGACAGCAACATGAGTGCTGTTGTGATCCAGAAAGCCAGACCCGCCAAGA AGCAGAAACACCAGCCAGGACATCTGCGCAGGGAAGCCTACGCAGATGATCTTCCACCCCCTCCAGTGCC ACCACCTGCTATAAAATCGCCCACTGTCCAGTCCAAGGCACAGCTGGAGGTACGGCCTGTCATGGTGCCA AAACTCGCGTCTATAGAAGCAAGGACAGATAGATCGTCAGACAGAAAAGGAGGCAGTTACAAGGGGAGAG AAGCTCTGGATGGAAGACAAGTCACTGACCTGCGAACAAATCCAAGTGACCCCAGAGAGGCACAGGAACA GCCAAATGACGGGAAAGGCCGAGGAACAAAGGCAGCCAAGCGAGACCTTCCACCAGCAAAGACGCACCTC GGCCAAGAGGATATTCTTCCGTACTGTAGACCTACTTTCCCAACGTCAAACAACCCTAGAGACCCCAGTT CCTCCAGCTCTATGTCATCAAGAGGATCAGGGAGCCGACAAAGAGAGCAAGCAAATGTGGGCCGAAGGAA TATGGCAGAAATGCAAGTACTTGGGGGATTTGAAAGAGGAGATGAGAATAATGAAGAATTAGAGGAAACT GAAAGC AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >MR224013 representing NM_019413
Red=Cloning site Green=Tags(s) MIAEPARFYLFGLICLCSGSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGERVE TDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPDEGVYICVARNYLGEAVSHNASLEVAILRDDFRQNPSDV MVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGER ESEVAELTVLERPSFVKRPSNLAVTVDDSAEFKCEARGDPVPTVRWRKDDGELPKSRYEIRDDHTLKIRK VTAGDMGSYTCVAENMVGKAEASATLTVQEPPHFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWRREGS QNLLFSYQPPQSSSRFSVSQTGDLTITNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPVIRQ GPVNQTVAVDGTLILSCVATGSPAPTILWRKDGVLVSTQDSRIKQLESGVLQIRYAKLGDTGRYTCTAST PSGEATWSAYIEVQEFGVPVQPPRPTDPNLIPSAPSKPEVTDVSKNTVTLSWQPNLNSGATPTSYIIEAF SHASGSSWQTAAENVKTETFAIKGLKPNAIYLFLVRAANAYGISDPSQISDPVKTQDVPPTSQGVDHKQV QRELGNVVLHLHNPTILSSSSVEVHWTVDQQSQYIQGYKILYRPSGASHGESEWLVFEVRTPTKNSVVIP DLRKGVNYEIKARPFFNEFQGADSEIKFAKTLEEAPSAPPRSVTVSKNDGNGTAILVTWQPPPEDTQNGM VQEYKVWCLGNETKYHINKTVDGSTFSVVIPSLVPGIRYSVEVAASTGAGPGVKSEPQFIQLDSHGNPVS PEDQVSLAQQISDVVRQPAFIAGIGAACWIILMVFSIWLYRHRKKRNGLTSTYAGIRKVPSFTFTPTVTY QRGGEAVSSGGRPGLLNISEPATQPWLADTWPNTGNNHNDCSINCCTAGNGNSDSNLTTYSRPADCIANY NNQLDNKQTNLMLPESTVYGDVDLSNKINEMKTFNSPNLKDGRFVNPSGQPTPYATTQLIQANLSNNMNN GAGDSSEKHWKPPGQQKPEVAPIQYNIMEQNKLNKDYRANDTIPPTIPYNQSYDQNTGGSYNSSDRGSST SGSQGHKKGARTPKAPKQGGMNWADLLPPPPAHPPPHSNSEEYNMSVDESYDQEMPCPVPPARMYLQQDE LEEEEDERGPTPPVRGAASSPAAVSYSHQSTATLTPSPQEELQPMLQDCPEDLGHMPHPPDRRRQPVSPP PPPRPISPPHTYGYISGPLVSDMDTDAPEEEEDEADMEVAKMQTRRLLLRGLEQTPASSVGDLESSVTGS MINGWGSASEEDNISSGRSSVSSSDGSFFTDADFAQAVAAAAEYAGLKVARRQMQDAAGRRHFHASQCPR PTSPVSTDSNMSAVVIQKARPAKKQKHQPGHLRREAYADDLPPPPVPPPAIKSPTVQSKAQLEVRPVMVP KLASIEARTDRSSDRKGGSYKGREALDGRQVTDLRTNPSDPREAQEQPNDGKGRGTKAAKRDLPPAKTHL GQEDILPYCRPTFPTSNNPRDPSSSSSMSSRGSGSRQREQANVGRRNMAEMQVLGGFERGDENNEELEET ES SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_019413 |
| ORF Size | 4836 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_019413.2, NP_062286.2 |
| RefSeq Size | 7568 bp |
| RefSeq ORF | 4839 bp |
| Locus ID | 19876 |
| UniProt ID | O89026 |
| MW | 176.8 kDa |
| Gene Summary | Receptor for SLIT1 and SLIT2 that mediates cellular responses to molecular guidance cues in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development (PubMed:10433822, PubMed:24560577). Interaction with the intracellular domain of FLRT3 mediates axon attraction towards cells expressing NTN1 (PubMed:24560577). In axon growth cones, the silencing of the attractive effect of NTN1 by SLIT2 may require the formation of a ROBO1-DCC complex (By similarity). Plays a role in the regulation of cell migration via its interaction with MYO9B; inhibits MYO9B-mediated stimulation of RHOA GTPase activity, and thereby leads to increased levels of active, GTP-bound RHOA (By similarity). May be required for lung development (PubMed:11734623).[UniProtKB/Swiss-Prot Function] |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC224754 | Robo1 (untagged) - Mouse roundabout homolog 1 (Drosophila) (Robo1), (10ug) |
CNY 11,040.00 |
|
| MG224013 | Robo1 (tGFP-tagged) - Mouse roundabout homolog 1 (Drosophila) (Robo1), (10ug) |
CNY 15,580.00 |
|
| MR224013L3 | Lenti ORF clone of Robo1 (Myc-DDK-tagged) - Mouse roundabout homolog 1 (Drosophila) (Robo1) |
CNY 16,060.00 |
|
| MR224013L4 | Lenti ORF clone of Robo1 (mGFP-tagged) - Mouse roundabout homolog 1 (Drosophila) (Robo1) |
CNY 16,060.00 |
