Abca3 (NM_013855) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	1810036E22Rik; ABC-C; Abc3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR224548 representing NM_013855 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGTGCTGCGGCAGCTCACCCTCCTACTCTGGAAGAACTATACCCTGAAGAAACGGAAGGTTCTAG TGACAGTCCTGGAGCTCTTCCTGCCCCTGCTGTTTTCTGGGATCCTGATTTGGCTTCGCTTGAAGATCCA GTCGGAGAATGTGCCCAATGCCACTGTTTACCCGGACCAGTCCATCCAGGAGCTGCCCCTGTTTTTCTCA TTCCCTCCACCTGGGGGGACCTGGGAGCTCGCCTATGTCCCTTCCCACAGCGATGCTGCCAGGACCATCA CGGAGACGGTGAAAAGGGAATTCATGATCAAGATGAGAGTGCACGGCTTCTCCTCTGAGAAGGACTTCGA GGACTACATTCGCTATGACAACCACTCCTCCAGCGTGCTGGCAGCTGTGGTATTTGAGCACAGCTTCAAC CACAGCCAGGACCCCCTGCCGCTGGCGGTGAAATACCACCTGCGCTTCAGTTACACACGAAGAAATTACA TGTGGACCCAAACAGGAAATATTTTCCTAAAAGAGACTGAAGGCTGGCATACCACTTCACTTTTTCCACT TTTTCCAAGCCCCGGACCGCGGGAACCTTCATCACCTGATGGCGGTGAACCTGGATACATTCGGGAAGGC TTCCTGGCCATGCAACATGCTGTAGACAAAGCCATCATGCGTTACCATGCCAACACCTCTGCACAGCAGC TGTTCCAGAAGCTCATGGTGATCACCAAAAGGTTCCCGTTCCCACCATACATCTCAGATCCATTCCTCAT TGCCATCCAGTACCAGCTTCCTCTGCTGCTCATGCTGAGTTTCACCTACACCTCCCTCACCATCATCCGG GCTGTGGTGCAGGAGAAGGAGAAAAAACTAAAGGAGTACATGCGCATGATGGGGCTTAACAGCTGGCTAC ACTGGAGCGCTTGGTTCCTCATGTTCTTCCTATTCTTCCTCATCGTGGTCTCCTTCATGACCCTCCTGTT CTGTGTCAAAGTGAAGAAGGACATAGCAGTCCTTTCCAACAGCGACCCCTCCCTGGTCCTGGCGTTCCTG CTGTGTTTTGCAATCTCCTCCATTTCCTTCAGCTTCATGGTCAGCACCTTCTTCAGTAAAGCCAACATAG CAGCAGCCGTGGGTGGCTTCCTGTACTTCTTCACCTATACCCCCTACTTTTTCGTGGCTCCTCGGTACAA CTGGATGACACTGAGTCAAAAGCTCTTGTCCTGTCTCCTATCTAATGTTGCCATGGCAATGGGAGCCCAG CTCATAGGAAAATTCGAAGCCAAAGGCACTGGCATCCAGTGGCGGGACCTCCTCAACCCTGTCAACGTGG ACGACAATTTCTGCTTTGGACAAGTGCTGGGGATGCTGCTGCTGGACTCAGCACTCTATGGCCTGGTGAC CTGGTACGTGGAGGCTGTCTTCCCAGGCCAGTTTGGCGTACCCCAGCCCTGGCACTTCTTCCTTATGCCC TCCTACTGGTGTGGGAACCCAAGAACTGTCGTAGGGAAGGAAGAGGAAGGCAGCGACCCAGAGAAAGCTC TCAGGAACGAGTACTTTGAAGCCGAGCCAGAGGACTTGGTAGCTGGAATCAAGATCAAGCATCTCTCCAA GGTGTTCCAAGTGGGCAATAAGGACAAGATGGGCATCAGAGACCTGACCCTGAACCTCTACGAAGGACAG ATAACAGTCCTCCTGGGCCACAATGGTGCTGGGAAGACCACGACCATGTCTCTGCTGACAGGCCTCTTCC CCCCTACCAGTGGACATGCTTATATTCATGGGTACGAAATTTCCCAAGACATGGCTCAGATCCGCAAGAG TTTGGGCCTGTGTCCCCAGCATGATGTACTGTTTGACAACCTGACAGTTGCCGAACATCTTTACTTCTAT GCACAGCTGAAAGGCCTCTCTCTCCAGAAGTGTCCTGAGGAAGTCAAGCAGATGCTACACATCCTCAGCC TGGAGGACAAGCGTGACTTACGGTCCAAGTTCCTGAGTGGGGGCATGAAGCGCAAGCTCTCCATTGGCAT CGCCCTCATAGCGGGCTCTAAGGTGCTGATGTTAGATGAGCCCACCTCAGGCATGGATGCAGTGTCCAGA AGGGCCATCTGGGACCTTCTCCAGCAGCAGAAGAGTGACCGCACCGTCCTGCTGACCACTCACTTCATGG ACGAAGCTGACCTGCTGGGAGACCGCATCGCCATCTTGGCCAAGGGGGAGCTCCAGTGCTGCGGGTCTTC ACTATTTCTCAAGCAGAAATACGGAGCTGGCTACCACATGACGCTGGTAAAAGAACCGCACTGCAATCCT GAAGGAATCTCTCAGCTGGTGCACCACCATGTCCCCAATGCCATGCTGGAGAGCCATGCTGGGGCTGAGC TGTCCTTTATTCTTCCCAAGGAGAGCACGCACAGGTTTGAAAGTCTGTTTGCTAAACTGGAAAAGAAGCA GAAGGAGTTGGGCATCGCAAGCTTCGGGGCCTCGGTCACCACCATGGAGGAAGTCTTCCTTCGGGTTGGT AAGCTGGTGGACACCAGCATGGACATTCAAGCCATTCAGCTTCCTGCTCTGCAGTACCAGCATGAGAGGC GGGCAAGCGACTGGGCTTTGGACAGTAACCTGTGTGGGGTGATGGACCCAACGAATGGCATTGGAGCCCT CATAGAGGAAGAGGAAGTTTTGGTCAAGCTCAACACAGGGCTTGCCCTCCACTGCCAGCAGTTCTGGGCC ATGTTCCTGAAGAAGGCTGCATACAGCTGGCGGGAATGGAAGATGGTGGCAGCTCAGGTCCTGGTCCCCC TGACCTGCCTCACCCTGGCCCTCCTGGCTATCCACTACACCTCAGAGATCTTTGATGACCCTCTCCTGAA GCTAAGCCTGAACGAGTATGGTAGAACGGTTGTGCCCTTCTCGGTCCCAGGCACATCCAGGCTGGCTCAG CAGCTGTCTGAGAATCTAAGGGACATGCTGCAGGCTGAGAGGCAGGAGCCCCGGGAAGTGCTAGGTGACC TAGAGGAGTTCCTGGTTTTCAGGGCCTCAGTGGAAGGAGGCGGCTTTAATGAGCGGTGCCTAGTAGCAAC ATCCTTCAAAGACAGAGGAGAGCTGACCGTGGTCACTGCCCTGTTCAACAACCAGGCATACCACTCCCCA GCCACTGCCCTGGCCATCGTGGACAACCTTCTGTTCAAGCTATTGTGTGGCCCTCAGGCCTCCATTGAGA TCTCCAACTACCCCCAGCCCCGGAACACCCTGCAGGTCGCCAAGGACCACTTCAATGAGGGCCGGAAGGG ATTTGACATTGCCCTCAATTTGCTCATCGCCATGGCATTTCTGGCCAGCACCTTTTCCATCCTGGCTGTC AGTGAGAGGGCCGTCCAGGCCAAGCACGTTCAGTTTGTGAGCGGTGTCCATGTGGCTACTTTCTGGTTCT CCGCTCTGTTGTGGGACCTCATCTCCTTCCTCGTTCCCAGTCTACTACTTCTGGTGGTGTTCCAAGCCTT CAATGTGCACGCCTTCACGAGGGATGGTCACATGGCTGACCTGCTGCTGCTGCTCATGCTCTACGGCTGG GCCATCATCCCCCTCATGTACCTCATGAGCTTCTTCTTCTCGGCTGCGTCCACAGCTTATACCAGGCTGA CCATATTCAACATCCTGTCGGGCATCGCTACCTTCATCATGGTCACTATCATGCGTATCCCAGCTGTGAA GTTAGAAGAACTTTCCAGAACCTTAGATCATGTGTTCTTGGTGCTGCCAAACCACTGCCTGGGGATGGCG GTCAGCAACTTCTATGAGAACTATGAGACTCGGCGATACTGCACTTCCTCAGAGCTTGCTGCCCACTACT GCAAGAAGTACAACATCCAGTACCAGGAGAGCTTCTATGCCTGGAGCACCCCAGGCGTGGGCAAGTTTGT GACTTCCATGGCTGCCTCAGGGGGCATCTACCTCACCCTGCTGTTCCTCATTGAGACCAACCTGCTGTGG CGACTGAGAACCTTCATCTGTGCCTTCCGGAGGAGGTGGACTCTGGCAGAACTGCAGAACCGGACATCAG TGCTGCCCGAGGACCAGGATGTAGCTGAGGAGAGGAGCCGAATCCTGGTCCCTAGCTTGGACTCCATGCT CGACACACCACTGATCATCAACGAGCTCTCCAAGGTGTATGACCAGCGAGCACCGCTCCTTGCCGTGGAC AGGATCTCCCTTGCGGTCCAGAAAGGGGAGTGCTTCGGCCTGTTGGGTTTCAATGGAGCTGGAAAAACCA CAACATTCAAAATGCTGACTGGGGAGGAGACCATCACCTCAGGGGATGCCTTTGTTGGTGGTTACAGCAT CAGTTCTGACATCGGGAAGGTGCGGCAGCGGATGGGCTACTGCCCCCAGTTTGATGCACTGCTTGATCAC ATGACTGGCAGGGAGATGCTGGTTATGTATGCACGGCTCCGAGGCATCCCAGAGCGGCTCATCAATGCCT GTGTGGAGAATACTCTGCGGGGTCTGCTGCTGGAACCGCACGCCAACAAACTAGTCAAGACTTACAGTGG TGGTAACAAACGCAAGCTAAGCACTGGCATTGCCCTCATTGGAGAGCCTGCAGTCATCTTCCTGGATGAG CCATCGACCGGCATGGACCCAGTGGCCCGGCGTCTACTCTGGGATACTGTAGCCCGGGCCCGAGAGTCTG GCAAAGCCATTGTCATCACCTCCCACAGTATGGAGGAGTGTGAAGCCTTATGTACCAGGCTGGCCATCAT GGTACAGGGCCAGTTCAAGTGCCTGGGCAGCCCACAGCACCTCAAGAGCAAGTTTGGCAGCGGCTACTCC CTGCAGGCCAAGGTCCGGAGCGAGGGGAAGCAAGACGCGCTAGAGGAGTTCAAGGCCTTTGTGGACCTGA CCTTCCCAGGCAGCATCCTAGAAGATGAGCACCAAGACATGGTCCATTATCATCTGCCTGGCTGTGACCT CAGCTGGGCCAAGGTATTTGGTATCCTGGAGAAAGCCAAGGAGAAGTATGGAGTGGATGATTACTCTGTG AGCCAGATCTCCCTGGAGCAGGTCTTTCTGAGCTTTGCCCACCTACAACCCCCCACCACTGAGGATGGCC GG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR224548 representing NM_013855 Red=Cloning site Green=Tags(s) MAVLRQLTLLLWKNYTLKKRKVLVTVLELFLPLLFSGILIWLRLKIQSENVPNATVYPDQSIQELPLFFS FPPPGGTWELAYVPSHSDAARTITETVKREFMIKMRVHGFSSEKDFEDYIRYDNHSSSVLAAVVFEHSFN HSQDPLPLAVKYHLRFSYTRRNYMWTQTGNIFLKETEGWHTTSLFPLFPSPGPREPSSPDGGEPGYIREG FLAMQHAVDKAIMRYHANTSAQQLFQKLMVITKRFPFPPYISDPFLIAIQYQLPLLLMLSFTYTSLTIIR AVVQEKEKKLKEYMRMMGLNSWLHWSAWFLMFFLFFLIVVSFMTLLFCVKVKKDIAVLSNSDPSLVLAFL LCFAISSISFSFMVSTFFSKANIAAAVGGFLYFFTYTPYFFVAPRYNWMTLSQKLLSCLLSNVAMAMGAQ LIGKFEAKGTGIQWRDLLNPVNVDDNFCFGQVLGMLLLDSALYGLVTWYVEAVFPGQFGVPQPWHFFLMP SYWCGNPRTVVGKEEEGSDPEKALRNEYFEAEPEDLVAGIKIKHLSKVFQVGNKDKMGIRDLTLNLYEGQ ITVLLGHNGAGKTTTMSLLTGLFPPTSGHAYIHGYEISQDMAQIRKSLGLCPQHDVLFDNLTVAEHLYFY AQLKGLSLQKCPEEVKQMLHILSLEDKRDLRSKFLSGGMKRKLSIGIALIAGSKVLMLDEPTSGMDAVSR RAIWDLLQQQKSDRTVLLTTHFMDEADLLGDRIAILAKGELQCCGSSLFLKQKYGAGYHMTLVKEPHCNP EGISQLVHHHVPNAMLESHAGAELSFILPKESTHRFESLFAKLEKKQKELGIASFGASVTTMEEVFLRVG KLVDTSMDIQAIQLPALQYQHERRASDWALDSNLCGVMDPTNGIGALIEEEEVLVKLNTGLALHCQQFWA MFLKKAAYSWREWKMVAAQVLVPLTCLTLALLAIHYTSEIFDDPLLKLSLNEYGRTVVPFSVPGTSRLAQ QLSENLRDMLQAERQEPREVLGDLEEFLVFRASVEGGGFNERCLVATSFKDRGELTVVTALFNNQAYHSP ATALAIVDNLLFKLLCGPQASIEISNYPQPRNTLQVAKDHFNEGRKGFDIALNLLIAMAFLASTFSILAV SERAVQAKHVQFVSGVHVATFWFSALLWDLISFLVPSLLLLVVFQAFNVHAFTRDGHMADLLLLLMLYGW AIIPLMYLMSFFFSAASTAYTRLTIFNILSGIATFIMVTIMRIPAVKLEELSRTLDHVFLVLPNHCLGMA VSNFYENYETRRYCTSSELAAHYCKKYNIQYQESFYAWSTPGVGKFVTSMAASGGIYLTLLFLIETNLLW RLRTFICAFRRRWTLAELQNRTSVLPEDQDVAEERSRILVPSLDSMLDTPLIINELSKVYDQRAPLLAVD RISLAVQKGECFGLLGFNGAGKTTTFKMLTGEETITSGDAFVGGYSISSDIGKVRQRMGYCPQFDALLDH MTGREMLVMYARLRGIPERLINACVENTLRGLLLEPHANKLVKTYSGGNKRKLSTGIALIGEPAVIFLDE PSTGMDPVARRLLWDTVARARESGKAIVITSHSMEECEALCTRLAIMVQGQFKCLGSPQHLKSKFGSGYS LQAKVRSEGKQDALEEFKAFVDLTFPGSILEDEHQDMVHYHLPGCDLSWAKVFGILEKAKEKYGVDDYSV SQISLEQVFLSFAHLQPPTTEDGR TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_013855
ORF Size	5112 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_013855.3, NP_038883.2
RefSeq Size	6453 bp
RefSeq ORF	5115 bp
Locus ID	27410
UniProt ID	Q8R420
MW	192.4 kDa
Gene Summary	Plays an important role in the formation of pulmonary surfactant, probably by transporting lipids such as cholesterol.[UniProtKB/Swiss-Prot Function]

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