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Opa1 (NM_133752) Mouse Tagged ORF Clone
CAT＃: MR225663
- TrueORF®
Opa1 (Myc-DDK-tagged) - Mouse optic atrophy 1 homolog (human) (Opa1), nuclear gene encoding mitochondrial protein, transcript variant 2
ORF Plasmid: tGFP
"NM_133752" in other vectors (4)
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CNY 7,040.00
CNY 7,130.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 1200011N24Rik; AI225888; AI847218; lilr3; mKIAA0567 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR225663 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGGCGAGCAGGTCGGGCGGCCGTGGCCTGTGAAGTCTGCCAATCCTTAGTGAAACACAGCTCTGGAA TACAAAGAAACGTACCGCTCCAAAAACTCCATCTGGTTTCACGAAGTATTTATCGTTCACATCATCCTGC CCTCAAGCTTCAAAGACCCCAACTAAGGACACCATTTCAGCAGTTCTCTTCTCTAACTCACCTTTCATTA CATAAATTGAAACTTTCTCCAATTAAATATGGCTACCAGCCCCGCAGGAACTTTTGGCCAGCAAGGTTAG CTGCAAGACTCTTAAAACTTCGATATATCATACTGGGATCTGCTGTTGGAGGTGGCTATACAGCCAAAAA AACCTTCGATGAATGGAAAGATATGATACCAGACCTTAGTGACTATAAGTGGATTGTGCCTGACTTTATA TGGGAAATTGATGAGTATATTGATTTGGAGAAAATTAGAAAAGCCCTTCCCAGCTCAGAAGACCTTGCCA GTTTAGCTCCCGACTTGGACAAGATTACTGAGAGCCTCAGCTTGTTGAAGGACTTCTTCACTGCAGGTTC ACCTGGAGAAACAGCATTTCGAGCAACAGATCATGGATCTGAAAGTGACAAGCATTACAGGAAGGTGTCA GACAAAGAAAAGATTGACCAACTTCAAGAAGAACTTCTGCATACTCAGTTAAAGTATCAGAGGATCTTGG AGCGCCTGGAAAAGGAGAACAAAGAGCTGAGGAAGCTGGTGCTGCAGAAGGACGACAAAGGCATCCACCA CAGGAAGCTCAAGAAATCTTTGATTGATATGTATTCTGAAGTTCTTGATGTTCTTTCTGATTATGATGCC AGTTACAATACACAAGATCACCTACCACGGGTTGTTGTGGTTGGAGATCAGAGTGCTGGGAAAACCAGTG TGCTGGAAATGATTGCTCAGGCCCGGATCTTCCCGAGAGGGTCCGGCGAGATGATGACACGCTCTCCAGT GAAGGTGACTCTCAGTGAAGGCCCTCACCATGTGGCCTTGTTTAAAGATAGCTCTCGGGAATTTGATCTC ACCAAGGAGGAAGATCTTGCAGCATTAAGACATGAAATCGAACTCCGAATGAGGAAAAATGTGAAAGAAG GTTGTACTGTTAGCCCCGAGACCATATCTCTAAATGTCAAAGGCCCTGGGCTGCAGAGGATGGTGCTCGT GGACTTGCCTGGTGTCATCAACACTGTGACATCAGGCATGGCTCCCGACACAAAGGAAACTATTTTCAGT ATCAGCAAAGCTTACATGCAGAATCCTAACGCCATCATCCTGTGCATCCAAGACGGATCCGTAGATGCTG AGCGCAGTATTGTTACAGACTTGGTCAGTCAAATGGATCCTCATGGAAGAAGAACCATATTTGTTTTGAC CAAAGTAGACCTGGCAGAAAAAAATGTAGCCAGTCCAAGCAGGATACAACAGATAATTGAAGGCAAGCTC TTCCCAATGAAAGCTCTGGGTTATTTTGCTGTCGTAACAGGAAAAGGAAACAGCTCTGAAAGTATTGAAG CTATAAGAGAGTATGAAGAGGAATTTTTTCAGAATTCAAAACTGCTAAAGACAAGCATGCTAAAGGCACA CCAGGTCACCACGAGAAATCTCAGCCTTGCTGTGTCAGACTGCTTTTGGAAAATGGTTCGAGAGTCAGTT GAACAACAGGCTGATAGTTTTAAAGCCACGCGCTTTAACCTAGAGACGGAATGGAAGAATAACTACCCGC GCCTGCGAGAGCTCGACAGGAATGAACTCTTTGAAAAAGCTAAAAATGAGATCCTCGATGAGGTCATCAG TCTGAGCCAGGTCACGCCAAAGCACTGGGAGGAAATCCTGCAGCAATCCCTGTGGGAACGAGTGTCAACA CATGTGATTGAGAACATCTACCTTCCAGCTGCCCAGACCATGAATTCGGGAACATTTAACACCACAGTAG ACATCAAGCTTAAACAGTGGACTGACAAGCAGCTTCCTAATAAAGCAGTCGAGGTTGCCTGGGAGACTCT ACAAGAGGAATTTTCCCGCTTCATGACAGAACCCAAAGGAAAGGAACACGACGACATATTTGACAAACTT AAGGAGGCTGTGAAGGAGGAGAGTATCAAGCGGCACAAGTGGAACGACTTTGCCGAGGATAGCTTGAGGG TTATTCAGCACAATGCTTTGGAAGACCGGTCCATATCAGATAAGCAACAGTGGGATGCAGCCATTTACTT CATGGAAGAGGCGCTTCAAGGTCGTCTCAAGGATACTGAAAATGCTATTGAAAACATGATTGGGCCAGAC TGGAAAAAGAGGTGGATGTACTGGAAGAATCGGACCCAAGAGCAGTGTGTTCACAACGAAACCAAGAACG AGTTGGAGAAGATGCTGAAGGTTAATGATGAGCACCCAGCTTACCTGGCAAGTGATGAGATTACCACAGT CCGGAAGAACCTGGAGTCTCGAGGAGTGGAAGTCGATCCAAGCTTGATTAAGGATACTTGGCATCAAGTT TATAGAAGACATTTCTTAAAAACAGCTCTAAATCATTGTAACCTTTGTCGCAGAGGTTTTTATTACTACC AGAGGCATTTTATAGATTCTGAGCTGGAATGCAATGACGTGGTCCTGTTTTGGCGAATACAGCGCATGCT CGCTATCACTGCCAATACATTAAGGCAGCAGCTTACAAACACTGAAGTTAGGCGACTAGAGAAAAACGTT AAAGAGGTATTAGAAGATTTTGCAGAAGACGGTGAGAAGAAGGTTAAATTGCTCACTGGCAAACGAGTTC AGCTGGCAGAAGATCTCAAGAAAGTTAGAGAAATTCAAGAAAAGCTTGATGCTTTCATTGAAGCTCTTCA CCAGGAGAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR225663 protein sequence
Red=Cloning site Green=Tags(s) MWRAGRAAVACEVCQSLVKHSSGIQRNVPLQKLHLVSRSIYRSHHPALKLQRPQLRTPFQQFSSLTHLSL HKLKLSPIKYGYQPRRNFWPARLAARLLKLRYIILGSAVGGGYTAKKTFDEWKDMIPDLSDYKWIVPDFI WEIDEYIDLEKIRKALPSSEDLASLAPDLDKITESLSLLKDFFTAGSPGETAFRATDHGSESDKHYRKVS DKEKIDQLQEELLHTQLKYQRILERLEKENKELRKLVLQKDDKGIHHRKLKKSLIDMYSEVLDVLSDYDA SYNTQDHLPRVVVVGDQSAGKTSVLEMIAQARIFPRGSGEMMTRSPVKVTLSEGPHHVALFKDSSREFDL TKEEDLAALRHEIELRMRKNVKEGCTVSPETISLNVKGPGLQRMVLVDLPGVINTVTSGMAPDTKETIFS ISKAYMQNPNAIILCIQDGSVDAERSIVTDLVSQMDPHGRRTIFVLTKVDLAEKNVASPSRIQQIIEGKL FPMKALGYFAVVTGKGNSSESIEAIREYEEEFFQNSKLLKTSMLKAHQVTTRNLSLAVSDCFWKMVRESV EQQADSFKATRFNLETEWKNNYPRLRELDRNELFEKAKNEILDEVISLSQVTPKHWEEILQQSLWERVST HVIENIYLPAAQTMNSGTFNTTVDIKLKQWTDKQLPNKAVEVAWETLQEEFSRFMTEPKGKEHDDIFDKL KEAVKEESIKRHKWNDFAEDSLRVIQHNALEDRSISDKQQWDAAIYFMEEALQGRLKDTENAIENMIGPD WKKRWMYWKNRTQEQCVHNETKNELEKMLKVNDEHPAYLASDEITTVRKNLESRGVEVDPSLIKDTWHQV YRRHFLKTALNHCNLCRRGFYYYQRHFIDSELECNDVVLFWRIQRMLAITANTLRQQLTNTEVRRLEKNV KEVLEDFAEDGEKKVKLLTGKRVQLAEDLKKVREIQEKLDAFIEALHQEK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_133752 |
| ORF Size | 2883 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_133752.1 |
| RefSeq Size | 5948 bp |
| RefSeq ORF | 2883 bp |
| Locus ID | 74143 |
| UniProt ID | P58281 |
| MW | 111.3 kDa |
| Gene Summary | Dynamin-related GTPase that is essential for normal mitochondrial morphology by regulating the equilibrium between mitochondrial fusion and mitochondrial fission (PubMed:11847212, PubMed:24616225, PubMed:26785494, PubMed:28746876). Coexpression of isoform 1 with shorter alternative products is required for optimal activity in promoting mitochondrial fusion (By similarity). Binds lipid membranes enriched in negatively charged phospholipids, such as cardiolipin, and promotes membrane tubulation. The intrinsic GTPase activity is low, and is strongly increased by interaction with lipid membranes (By similarity). Plays a role in remodeling cristae and the release of cytochrome c during apoptosis (PubMed:16839884, PubMed:16839885). Proteolytic processing in response to intrinsic apoptotic signals may lead to disassembly of OPA1 oligomers and release of the caspase activator cytochrome C (CYCS) into the mitochondrial intermembrane space (PubMed:16839884, PubMed:16839885). Plays a role in mitochondrial genome maintenance (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC222838 | Opa1 (untagged) - Mouse optic atrophy 1 homolog (human) (Opa1), nuclear gene encoding mitochondrial protein, transcript variant 2, (10ug) |
CNY 12,260.00 |
|
| MG225663 | Opa1 (tGFP-tagged) - Mouse optic atrophy 1 homolog (human) (Opa1) nuclear gene encoding mitochondrial protein, (10ug) |
CNY 7,890.00 |
|
| MR225663L3 | Lenti ORF clone of Opa1 (Myc-DDK-tagged) - Mouse optic atrophy 1 homolog (human) (Opa1), nuclear gene encoding mitochondrial protein, transcript variant 2 |
CNY 9,030.00 |
|
| MR225663L4 | Lenti ORF clone of Opa1 (mGFP-tagged) - Mouse optic atrophy 1 homolog (human) (Opa1), nuclear gene encoding mitochondrial protein, transcript variant 2 |
CNY 9,030.00 |
