Il18r1 (NM_008365) Mouse Tagged ORF Clone

CAT#: MR225765

  • TrueORF®

Il18r1 (Myc-DDK-tagged) - Mouse interleukin 18 receptor 1 (Il18r1), transcript variant 1

ORF Plasmid: DDK tGFP



  "NM_008365" in other vectors (4)

Need custom modification / cloning service?
Get a free quote

Mouse Protein / Lysate Request

CNY 6,000.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (3)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms Il1rrp; Il18ralpha
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR225765 representing NM_008365
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCATCATGAAGAATTAATCTTGACACTCTGCATTCTCATTGTTAAAAGTGCCTCAAAAAGTTGTATTC
ACCGATCACAAATTCATGTGGTAGAGGGAGAACCTTTTTATCTGAAGCCATGTGGCATATCTGCACCAGT
GCACAGGAATGAAACAGCCACCATGAGATGGTTCAAAGGCAGTGCTTCACATGAGTATAGAGAGCTGAAC
AACAGAAGCTCGCCCAGAGTCACTTTTCATGATCACACCTTGGAATTCTGGCCAGTTGAGATGGAGGATG
AGGGAACGTACATTTCTCAAGTCGGAAATGATCGTCGCAATTGGACCTTAAATGTCACCAAAAGAAACAA
ACACAGCTGTTTCTCTGACAAGCTCGTGACAAGCAGAGATGTTGAAGTTAACAAATCTCTGCATATCACT
TGTAAGAATCCTAACTATGAAGAGCTGATCCAGGACACATGGCTGTATAAGAACTGTAAGGAAATATCCA
AAACCCCAAGGATCCTGAAGGATGCCGAGTTTGGAGATGAGGGCTACTACTCCTGCGTGTTTTCTGTCCA
CCATAATGGGACACGGTACAACATCACCAAGACTGTCAATATAACAGTTATTGAAGGAAGGAGTAAAGTA
ACTCCAGCTATTTTAGGACCAAAGTGTGAGAAGGTTGGTGTAGAACTAGGAAAGGATGTGGAGTTGAACT
GCAGTGCTTCATTGAATAAAGACGATCTGTTTTATTGGAGCATCAGGAAAGAGGACAGCTCAGACCCTAA
TGTGCAAGAAGACAGGAAGGAGACGACAACATGGATTTCTGAAGGCAAACTGCATGCTTCAAAAATACTG
AGATTTCAGAAAATTACTGAAAACTATCTCAATGTTTTATATAATTGCACCGTGGCCAACGAAGAAGCCA
TAGACACCAAGAGCTTCGTCTTGGTGAGAAAAGAAATACCTGATATCCCAGGCCATGTCTTTACAGGAGG
AGTAACTGTGCTTGTTCTCGCCTCTGTGGCAGCAGTGTGTATAGTGATTTTGTGTGTCATTTATAAAGTT
GACTTGGTTCTGTTCTATAGGCGCATAGCGGAAAGAGACGAGACACTAACAGATGGTAAAACATATGATG
CCTTTGTGTCTTACCTGAAAGAGTGTCATCCTGAGAATAAAGAAGAGTATACTTTTGCTGTGGAGACGTT
ACCCAGGGTCCTGGAGAAACAGTTTGGGTATAAGTTATGCATATTTGAAAGAGATGTGGTGCCTGGCGGA
GCTGTTGTCGAGGAGATCCATTCACTGATAGAGAAAAGCCGGAGGCTAATCATCGTTCTCAGCCAGAGTT
ACCTGACTAACGGAGCCAGGCGTGAGCTCGAGAGTGGACTCCACGAAGCACTGGTAGAGAGGAAGATTAA
GATCATCTTAATTGAGTTTACTCCAGCCAGCAACATCACCTTTCTCCCCCCGTCGCTGAAACTCCTGAAG
TCCTACAGAGTTCTAAAATGGAGGGCTGACAGTCCCTCCATGAACTCAAGGTTCTGGAAGAATCTTGTTT
ACCTGATGCCCGCAAAAGCCGTCAAGCCATGGAGAGAGGAGTCGGAGGCGCGGTCTGTTCTCTCAGCACC
T


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR225765 representing NM_008365
Red=Cloning site Green=Tags(s)

MHHEELILTLCILIVKSASKSCIHRSQIHVVEGEPFYLKPCGISAPVHRNETATMRWFKGSASHEYRELN
NRSSPRVTFHDHTLEFWPVEMEDEGTYISQVGNDRRNWTLNVTKRNKHSCFSDKLVTSRDVEVNKSLHIT
CKNPNYEELIQDTWLYKNCKEISKTPRILKDAEFGDEGYYSCVFSVHHNGTRYNITKTVNITVIEGRSKV
TPAILGPKCEKVGVELGKDVELNCSASLNKDDLFYWSIRKEDSSDPNVQEDRKETTTWISEGKLHASKIL
RFQKITENYLNVLYNCTVANEEAIDTKSFVLVRKEIPDIPGHVFTGGVTVLVLASVAAVCIVILCVIYKV
DLVLFYRRIAERDETLTDGKTYDAFVSYLKECHPENKEEYTFAVETLPRVLEKQFGYKLCIFERDVVPGG
AVVEEIHSLIEKSRRLIIVLSQSYLTNGARRELESGLHEALVERKIKIILIEFTPASNITFLPPSLKLLK
SYRVLKWRADSPSMNSRFWKNLVYLMPAKAVKPWREESEARSVLSAP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_008365
ORF Size 1611 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_008365.2, NP_032391.1
RefSeq Size 4158 bp
RefSeq ORF 1614 bp
Locus ID 16182
UniProt ID Q61098
MW 62.1 kDa
Gene Summary Within the IL18 receptor complex, responsible for the binding of the proinflammatory cytokine IL18, but not IL1A nor IL1B. Involved in IL18-mediated IFNG synthesis from T-helper 1 (Th1) cells (By similarity). Contributes to IL18-induced cytokine production, either independently of SLC12A3, or as a complex with SLC12A3 (PubMed:26099046).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Other Versions

Customer Reviews 
Loading...