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Adcy7 (NM_001109756) Mouse Tagged ORF Clone
CAT＃: MR226308
- TrueORF®
Adcy7 (Myc-DDK-tagged) - Mouse adenylate cyclase 7 (Adcy7), transcript variant 4
ORF Plasmid: tGFP
"NM_001109756" in other vectors (4)
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CNY 7,656.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AA407758 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR226308 representing NM_001109756
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCAGCCAAGGGGCGCTACTTCCTAAATGAGGGTGATGAAGGCCCCGACCAGGCAGCGCTCTATGAGA AGTACCGGCTCACCAGCTTGCACGGGCCACTGCTGCTCTTGCTCCTCCTGGTGGCCGCGGCCACCTGCAT TGCGCTCATCAGCATCGCCTTCAGTCATGAGGATCTCCGCAGACACCAGGTTGTCCTGGGCACTGCGTTC CTCATGCTGACGCTGTTTGTGGCTCTCTATGTGCTGGTGTATGTCGAGTGCCTGGTGCAGCGGTGGCTGC GGGCCTTGGCGCTACTCACCTGGGCTTGCCTCATGGTACTAGGCTCCGTGCTGATGTGGGACTCTTTGGA GAATGAAGCCCATGCGTGGGAGCAGGTGCCTTTCTTCCTGTTTGTCGTCTTTGTGGTGTATGCACTACTG CCTCTCAGCAGGAGGGCAGCCATCGTGGCAGGCGTGACCTCCACGGTCTCCCATCTCCTGGTGTTTGGAG CTGTGACAAGAGCCTTCCAGACGTCCATGTCTAGCACTCAACTGGGGCTGCAGCTCCTGGCCAATGCCGT TATCCTCCTGGGTGGGAACTTCACGGGTGCCTTCCACAAGCACCAGCTGCAGGACGCGTCCAGGGATCTC TTTATCTACACCGTCAAATGCATCCAGATCCGTCGGAAGCTTCGTGTGGAGAAGCGCCAGCAGGAGAACC TGCTTCTGTCAGTGCTCCCAGCACACATCTCCATGGGTATGAAGCTGGCCATCATTGAGCGCCTCAAAGA GGGTGGTGACCGACACTACATGCCCGACAACAACTTTCACAGCCTCTATGTCAAGCGGCACCAGAATGTC AGCATCTTGTATGCAGACATCGTGGGCTTCACGAGGCTGGCCAGCGACTGCTCTCCCAAGGAGCTGGTGG TGGTGCTCAACGAGCTGTTTGGGAAGTTTGACCAGATTGCTAAGGCCAATGAGTGCATGCGGATCAAGAT CCTGGGTGACTGTTACTACTGCGTGTCAGGCCTGCCCGTGTCGCTGCCCACACATGCCCGCAACTGTGTG AAGATGGGTCTGGACATCTGCGAGGCCATTAAGCAGGTGCGTGAGGCCACGGGCGTGGACATCAGCATGC GTGTGGGCATTCACTCCGGGAATGTGCTATGTGGGGTCATCGGGCTCCGTAAGTGGCAGTATGATGTGTG GTCCCATGATGTGTCCCTGGCCAACAGGATGGAGGCAGCTGGAGTCCCTGGCCGGGTGCACATCACAGAG GCAACATTGAATCACCTGGACAAGGCATATGAGGTGGAGGATGGGCATGGGGAGCAGCGAGACCCCTATC TGAAAGAGATGAACATCCGAACCTACCTGGTGATCGATCCCCGGAGCCAGCAGCCACCCCCACCCAGCCA CCACCTCTCCAAGCCCAAGGGGGACGCAACTCTGAAGATGCGGGCTTCAGTGCGTGTAACCCGCTATCTG GAGTCTTGGGGGGCAGCAAGGCCCTTTGCACACCTCAACCACCGGGAGAGTGTGAGCAGCAGTGAGACCC CCATCTCCAATGGACGGAGGCAGAAGGCCATTCCTCTGCGTCGACACCGTGCCCCTGATAGGAGTGCATC TCCCAAGGGGCGCTTGGAAGATGACTGTGATGACGAGATGCTGTCAGCCATTGAGGGTCTCAGCTCCACC AGGCCCTGCTGCTCCAAGTCTGATGACTTCCACACCTTTGGTCCCATCTTCTTGGAGAAGGGCTTTGAGC GTGAGTACCGCCTGGTGCCCATCCCCCGGGCTCGCTACGACTTCGCCTGTGCCAGCCTTGTCTTCGTCTG CATCCTGCTTGTCCACCTTCTAGTGATGCCCAGGATGGCAACTCTGGGTGTGTCCTTTGGGTTGGTGGCC TGCCTGCTGGGGCTGGTTCTGAGTTTCTGCTTTGCTACTGAGTTCTCGAGGTGCTTTCCATCCCGAAGTA CACTCCAGGCCATCTCGGAGAGCGTGGAGACGCAGCCCCTGGTCAGGCTTGTCCTGGTTGTGCTGACTGT TGGCAGCCTACTGACTGTCGCCATCATTAACATGCCACTGACGCTTAACCCAGGCCCAGAGCAGCCTGGA GACAACAAGACAAGCCCACTGGCTGCACAGAACAGAGTTGGGACCCCATGTGAGCTCCTCCCGTACTACA CCTGCAGCTGCATCCTGGGCTTCATTGCATGCTCTGTTTTCCTGCGGATGAGCCTAGAGCTGAAGGCCAT GCTGCTGACAGTGGCCTTGGTGGCCTACCTGCTGCTCTTCAACCTCTCCCCATGCTGGCACGTCTCAGGC AACAGCACTGAGACCAACGGGACACAAAGGACACGGCTGCTCCTGTCTGATGCACAAAGCATGCCCAGCC ACACCCTTGCTCCGGGGGCTCGGGAGACTGCCCCTTCTCCCAGTTATTTAGAGAGAGACCTGAAGATCAT GGTTAACTTCTACCTGATCCTGTTCTATGCCACCCTCATCTTGCTGTCTAGACAGATTGACTACTACTGC CGCTTGGACTGTCTGTGGAAGAAGAAGTTCAAAAAGGAGCACGAGGAGTTTGAAACAATGGAGAATGTGA ACCGCCTCCTCCTGGAGAATGTGCTGCCGGCGCACGTGGCTGCCCACTTCATTGGGGACAAGGCAGCAGA GGATTGGTACCATCAATCTTATGACTGTGTCTGTGTCATGTTTGCATCCGTTCCGGACTTCAAAGTGTTC TACACTGAGTGTGATGTCAACAAAGAAGGACTGGAGTGCCTTCGACTGCTGAATGAGATAATTGCTGATT TTGACGAGCTCCTGCTGAAGCCCAAGTTTAGTGGTGTGGAGAAGATCAAGACCATTGGCAGCACCTACAT GGCGGCAGCAGGGCTCAGTGCCCCCTCAGGACATGAGAACCAGGACCTGGAGCGGAAGCACGTGCACATC GGAGTCTTGGTAGAATTTAGCATGGCCCTGATGAGCAAGCTGGATGGGATCAACAGGCACTCCTTCAACT CCTTCCGCCTCCGAGTCGGCATAAACCACGGGCCTGTGATTGCTGGAGTGATTGGAGCACGCAAGCCTCA GTATGACATCTGGGGAAACACAGTCAATGTTGCCAGCCGCATGGAGAGCACCGGAGAGCTTGGGAAAATC CAGGTTACCGAAGAGACATGCACTATCCTCCAGGGACTCGGATATTCGTGTGAATGCCGTGGGCTGATCA ACGTCAAAGGCAAAGGGGAACTGCGGACTTACTTTGTATGTACAGACACTGCCAAGTTTCAAGGGCTGGG GCTAAAC AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >MR226308 representing NM_001109756
Red=Cloning site Green=Tags(s) MPAKGRYFLNEGDEGPDQAALYEKYRLTSLHGPLLLLLLLVAAATCIALISIAFSHEDLRRHQVVLGTAF LMLTLFVALYVLVYVECLVQRWLRALALLTWACLMVLGSVLMWDSLENEAHAWEQVPFFLFVVFVVYALL PLSRRAAIVAGVTSTVSHLLVFGAVTRAFQTSMSSTQLGLQLLANAVILLGGNFTGAFHKHQLQDASRDL FIYTVKCIQIRRKLRVEKRQQENLLLSVLPAHISMGMKLAIIERLKEGGDRHYMPDNNFHSLYVKRHQNV SILYADIVGFTRLASDCSPKELVVVLNELFGKFDQIAKANECMRIKILGDCYYCVSGLPVSLPTHARNCV KMGLDICEAIKQVREATGVDISMRVGIHSGNVLCGVIGLRKWQYDVWSHDVSLANRMEAAGVPGRVHITE ATLNHLDKAYEVEDGHGEQRDPYLKEMNIRTYLVIDPRSQQPPPPSHHLSKPKGDATLKMRASVRVTRYL ESWGAARPFAHLNHRESVSSSETPISNGRRQKAIPLRRHRAPDRSASPKGRLEDDCDDEMLSAIEGLSST RPCCSKSDDFHTFGPIFLEKGFEREYRLVPIPRARYDFACASLVFVCILLVHLLVMPRMATLGVSFGLVA CLLGLVLSFCFATEFSRCFPSRSTLQAISESVETQPLVRLVLVVLTVGSLLTVAIINMPLTLNPGPEQPG DNKTSPLAAQNRVGTPCELLPYYTCSCILGFIACSVFLRMSLELKAMLLTVALVAYLLLFNLSPCWHVSG NSTETNGTQRTRLLLSDAQSMPSHTLAPGARETAPSPSYLERDLKIMVNFYLILFYATLILLSRQIDYYC RLDCLWKKKFKKEHEEFETMENVNRLLLENVLPAHVAAHFIGDKAAEDWYHQSYDCVCVMFASVPDFKVF YTECDVNKEGLECLRLLNEIIADFDELLLKPKFSGVEKIKTIGSTYMAAAGLSAPSGHENQDLERKHVHI GVLVEFSMALMSKLDGINRHSFNSFRLRVGINHGPVIAGVIGARKPQYDIWGNTVNVASRMESTGELGKI QVTEETCTILQGLGYSCECRGLINVKGKGELRTYFVCTDTAKFQGLGLN SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001109756 |
| ORF Size | 3297 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001109756.1, NP_001103226.1 |
| RefSeq Size | 5937 bp |
| RefSeq ORF | 3300 bp |
| Locus ID | 11513 |
| UniProt ID | P51829 |
| MW | 123.2 kDa |
| Gene Summary | Catalyzes the formation of cAMP in response to activation of G protein-coupled receptors (Probable). Functions in signaling cascades activated namely by thrombin and sphingosine 1-phosphate and mediates regulation of cAMP synthesis through synergistic action of the stimulatory G alpha protein with GNA13 (PubMed:18541530). Also, during inflammation, mediates zymosan-induced increase intracellular cAMP, leading to protein kinase A pathway activation in order to modulate innate immune responses through heterotrimeric G proteins G(12/13) (PubMed:23178822). Functions in signaling cascades activated namely by dopamine and C5 alpha chain and mediates regulation of cAMP synthesis through synergistic action of the stimulatory G protein with G beta:gamma complex (By similarity). Functions, through cAMP response regulation, to keep inflammation under control during bacterial infection by sensing the presence of serum factors, such as the bioactive lysophospholipid (LPA) that regulate LPS-induced TNF-alpha production. However, it is also required for the optimal functions of B and T cells during adaptive immune responses by regulating cAMP synthesis in both B and T cells (PubMed:20505140).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223539 | Adcy7 (untagged) - Mouse adenylate cyclase 7 (Adcy7), transcript variant 4, (10ug) |
CNY 14,060.00 |
|
| MG226308 | Adcy7 (tGFP-tagged) - Mouse adenylate cyclase 7 (Adcy7) transcript variant 4, (10ug) |
CNY 9,220.00 |
|
| MR226308L3 | Lenti ORF clone of Adcy7 (Myc-DDK-tagged) - Mouse adenylate cyclase 7 (Adcy7), transcript variant 4 |
CNY 10,260.00 |
|
| MR226308L4 | Lenti ORF clone of Adcy7 (mGFP-tagged) - Mouse adenylate cyclase 7 (Adcy7), transcript variant 4 |
CNY 10,260.00 |
