Mmp9 (NM_013599) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	AW743869; B/MMP; B/MMP9; Clg4; Clg4b; Gel B; MMP-; MMP-9; pro-MMP-9
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR226712 representing NM_013599 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTCCCTGGCAGCCCCTGCTCCTGGCTCTCCTGGCTTTCGGCTGCAGCTCTGCTGCCCCTTACCAGC GCCAGCCGACTTTTGTGGTCTTCCCCAAAGACCTGAAAACCTCCAACCTCACGGACACCCAGCTGGCAGA GGCATACTTGTACCGCTATGGTTACACCCGGGCCGCCCAGATGATGGGAGAGAAGCAGTCTCTACGGCCG GCTTTGCTGATGCTTCAGAAGCAGCTCTCCCTGCCCCAGACTGGTGAGCTGGACAGCCAGACACTAAAGG CCATTCGAACACCACGCTGTGGTGTCCCAGACGTGGGTCGATTCCAAACCTTCAAAGGCCTCAAGTGGGA CCATCATAACATCACATACTGGATCCAAAACTACTCTGAAGACTTGCCGCGAGACATGATCGATGACGCC TTCGCGCGCGCCTTCGCGGTGTGGGGCGAGGTGGCACCCCTCACCTTCACCCGCGTGTACGGACCCGAAG CGGACATTGTCATCCAGTTTGGTGTCGCGGAGCACGGAGACGGGTATCCCTTCGACGGCAAGGACGGCCT TCTGGCACACGCCTTTCCCCCTGGCGCCGGCGTTCAGGGAGATGCCCATTTCGACGACGACGAGTTGTGG TCGCTGGGCAAAGGCGTCGTGATCCCCACTTACTATGGAAACTCAAATGGTGCCCCATGTCACTTTCCCT TCACCTTCGAGGGACGCTCCTATTCGGCCTGCACCACAGACGGCCGCAACGACGGCACGCCTTGGTGTAG CACAACAGCTGACTACGATAAGGACGGCAAATTTGGTTTCTGCCCTAGTGAGAGACTCTACACGGAGCAC GGCAACGGAGAAGGCAAACCCTGTGTGTTCCCGTTCATCTTTGAGGGCCGCTCCTACTCTGCCTGCACCA CTAAAGGCCGCTCGGATGGTTACCGCTGGTGCGCCACCACAGCCAACTATGACCAGGATAAACTGTATGG CTTCTGCCCTACCCGAGTGGACGCGACCGTAGTTGGGGGCAACTCGGCAGGAGAGCTGTGCGTCTTCCCC TTCGTCTTCCTGGGCAAGCAGTACTCTTCCTGTACCAGCGACGGCCGCAGGGATGGGCGCCTCTGGTGTG CGACCACATCGAACTTCGACACTGACAAGAAGTGGGGTTTCTGTCCAGACCAAGGGTACAGCCTGTTCCT GGTGGCAGCGCACGAGTTCGGCCATGCACTGGGCTTAGATCATTCCAGCGTGCCGGAAGCGCTCATGTAC CCGCTGTATAGCTACCTCGAGGGCTTCCCTCTGAATAAAGACGACATAGACGGCATCCAGTATCTGTATG GTCGTGGCTCTAAGCCTGACCCAAGGCCTCCAGCCACCACCACAACTGAACCACAGCCGACAGCACCTCC CACTATGTGTCCCACTATACCTCCCACGGCCTATCCCACAGTGGGCCCCACGGTTGGCCCTACAGGCGCC CCCTCACCTGGCCCCACAAGCAGCCCGTCACCTGGCCCTACAGGCGCCCCCTCACCTGGCCCTACAGCGC CCCCTACTGCGGGCTCTTCTGAGGCCTCTACAGAGTCTTTGAGTCCGGCAGACAATCCTTGCAATGTGGA TGTTTTTGATGCTATTGCTGAGATCCAGGGCGCTCTGCATTTCTTCAAGGACGGTTGGTACTGGAAGTTC CTGAATCATAGAGGAAGCCCATTACAGGGCCCCTTCCTTACTGCCCGCACGTGGCCAGCCCTGCCTGCAA CGCTGGACTCCGCCTTTGAGGATCCGCAGACCAAGAGGGTTTTCTTCTTCTCTGGACGTCAAATGTGGGT GTACACAGGCAAGACCGTGCTGGGCCCCAGGAGTCTGGATAAGTTGGGTCTAGGCCCAGAGGTAACCCAC GTCAGCGGGCTTCTCCCGCGTCGTCTCGGGAAGGCTCTGCTGTTCAGCAAGGGGCGTGTCTGGAGATTCG ACTTGAAGTCTCAGAAGGTGGATCCCCAGAGCGTCATTCGCGTGGATAAGGAGTTCTCTGGTGTGCCCTG GAACTCACACGACATCTTCCAGTACCAAGACAAAGCCTATTTCTGCCATGGCAAATTCTTCTGGCGTGTG AGTTTCCAAAATGAGGTGAACAAGGTGGACCATGAGGTGAACCAGGTGGACGACGTGGGCTACGTGACCT ACGACCTCCTGCAGTGCCCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR226712 representing NM_013599 Red=Cloning site Green=Tags(s) MSPWQPLLLALLAFGCSSAAPYQRQPTFVVFPKDLKTSNLTDTQLAEAYLYRYGYTRAAQMMGEKQSLRP ALLMLQKQLSLPQTGELDSQTLKAIRTPRCGVPDVGRFQTFKGLKWDHHNITYWIQNYSEDLPRDMIDDA FARAFAVWGEVAPLTFTRVYGPEADIVIQFGVAEHGDGYPFDGKDGLLAHAFPPGAGVQGDAHFDDDELW SLGKGVVIPTYYGNSNGAPCHFPFTFEGRSYSACTTDGRNDGTPWCSTTADYDKDGKFGFCPSERLYTEH GNGEGKPCVFPFIFEGRSYSACTTKGRSDGYRWCATTANYDQDKLYGFCPTRVDATVVGGNSAGELCVFP FVFLGKQYSSCTSDGRRDGRLWCATTSNFDTDKKWGFCPDQGYSLFLVAAHEFGHALGLDHSSVPEALMY PLYSYLEGFPLNKDDIDGIQYLYGRGSKPDPRPPATTTTEPQPTAPPTMCPTIPPTAYPTVGPTVGPTGA PSPGPTSSPSPGPTGAPSPGPTAPPTAGSSEASTESLSPADNPCNVDVFDAIAEIQGALHFFKDGWYWKF LNHRGSPLQGPFLTARTWPALPATLDSAFEDPQTKRVFFFSGRQMWVYTGKTVLGPRSLDKLGLGPEVTH VSGLLPRRLGKALLFSKGRVWRFDLKSQKVDPQSVIRVDKEFSGVPWNSHDIFQYQDKAYFCHGKFFWRV SFQNEVNKVDHEVNQVDDVGYVTYDLLQCP TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_013599
ORF Size	2190 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_013599.4, NP_038627.1
RefSeq Size	3185 bp
RefSeq ORF	2193 bp
Locus ID	17395
UniProt ID	P41245
MW	81 kDa
Gene Summary	This gene encodes a member of the matrix metalloproteinase family of extracellular matrix-degrading enzymes that are involved in tissue remodeling, wound repair, progression of atherosclerosis and tumor invasion. The encoded preproprotein undergoes proteolytic processing to generate a mature, zinc-dependent endopeptidase enzyme that degrades collagens of type IV, V and XI, and elastin. Mice lacking the encoded protein exhibit an abnormal pattern of skeletal growth plate vascularization and ossification, reduced keratinocyte hyperproliferation at all neoplastic stages, a decreased incidence of invasive tumors, and resistance to experimental autoimmune encephalomyelitis. [provided by RefSeq, Feb 2016]

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