Phlpp1 (NM_133821) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	AI836256; mKIAA0606; Phlpp; Plekhe1; SCOP
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR226995 representing NM_133821 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCCCGCCGCTGCAGCCCCGGCACAGCGACTCGCGGACCCCACGGGTGAGGACCAAGCCCTGGCGG CGGCGGCGGCCGAGGGCGGCCGGTGTCCGGATCCCGCGCTGAGCGCGGCGGCCCCGAGCGGCGGGAACGG CGGCGCGGCGCGAGAGGAGGCCCCCTGCGAGGCGCCGCCGGGGCCGCTGCCCGGCCGCGCCGGAGGCACC GGGCGCAGGCGGCGGCGGGGAGCTCCCCAGCCCGCGGCCGGTGGGGCCGCTCCGGTGCCCGCGGCCGGCG GCGGCGCCAACTCGCTGCTGCTGAAGAGAGGGCGGCTGAAGAGGAATCTGTCGGCCGCCGCCGCCGCCTC GTCGTCCTCGTCGCCGTCCTCGGCGTCTTCGGCCGCTGGCGGCCTCCCGGCGTCCTGCTCGGCCTCGGCG TCGCTGTGCACCCGGAGCCTGGACAGGAAGACGCTGCTCCTGAAGCACCGGCAGCTGCTTCAGCTGCAGC CGTCGGACAGGGACTGGGTGAGGCACCAGCTCCAGCGGGGCTGTGTGCACGTCTTTGACCGCCACATGGC CTCGTCCTACCTGCGCCCAGTGCTCTGCACGCTGGACACCACGGCCGCCGAGGTGGCGGCCCGGCTTCTG CAGCTGGGCCACAAAGGCGGCGGGGTGGTGAAGGTGCTGGGCTACGGGCCGCCTCCCGCCGCCGCCCCTG CCGCCAGCGATCAGACCCTGGACGGAGAGCACGGAAGGGACGTGGAGCCACCGCCCTCGAGCGGCACTGT TGGTGCTGTCCGGGGCCCTGCGCGCGCGCCTCCCGCCGATCTGCCGCTGCCCGGGGGCGCCTGGACGCGC TGTGCACCCCGCATCAGCCCCGCGCCTTCGGACTCCAGCCCCGGAGAGCTGTTCGCGGGCGGCCCCGGGT CCCCTCCCCGCGCCCCGCGCCCGGCCTCTGACACCGAGAGCTTCAGCCTGAGCCCCAGCGCCGAGAGCGT GTCAGACCGGCTGGACCCCTACAGCAGCGGCGGCGGCGGCTCCTCGTCGTCGTCGGAGGAGTTAGAGGCT GATCCGGCCATGCCCCACCGCCCCGGCCGCCCCGCGCAGCCTCGGCCGCCTTCCCCGAAGACCTCGGCTT TACTGCAGCCGAAAGCCCCGACGGGCGTAGACAGCACTGGCGTGATAGCGGGCGAGGGTCCCGGAGACGA TAAGGCGATGGCAGCCGCAGCTCCGGACGTTCCTCTGTCGACTTCGGGGAGGATACGGGAGACCGTGCAA AAGACATCTCCGCCCTCCCTGTATGTACAGCTCCATGGAGAGACTACCCGGCGCCTGGAGGCGGACGAGA AGCCATTGCAGATCCAAAATGACTACCTCTTCCAACTGGGATTTGGGGAGCTGTGGAGGGTGCAGGAGGA AGGCATGGACTCAGAGATTGGCTGTCTCATTCGATTCTATGCAGGAAAGCCTCATAGCACTGGGAGCTCT GAGCGGATTCAGCTCTCCGGAATGTACAATGTCCGAAAAGGCAAAATGCAGTTGCCAGTGAACCGATGGA CAAGACGACAGGTCATTCTGTGCGGGACGTGCTTGATAGTGTCGTCCGTGAAAGACAGCGTGAGTGGGAA GATGCATGTCCTGCCCCTCATTGGTGGAAAAGTGGAGGAAGTGAAAAAGCACCAGCACTGTTTAGCGTTC AGCTCTTCGGGGCCTCAAAGCCAGACTTACTACATCTGCTTTGATACTTTCACGGAGTACTTACGGTGGC TGCGGCAAGTCTCCAAGGTTGCATCACAGCGTATAAGCTCAGTAGACCTCTCCTGTTGTAGCCTTGAACA TCTGCCTGCCAACCTCTTTTACAGCCAAGACCTTACTCATCTCAACTTAAAACAGAACTTCCTAAGGCAA ACCCCCACCCTCCCAGCTGCCAGAGGACTTGGTGAACTGCAAAGGTTCACCAAATTGAAGAGCCTTAACC TTTCCAATAACCACCTAGGAGCCTTTCCGTCAGCAGTCTGCAGCATTCCAACCCTAGCAGAGCTGAATGT ATCTTGCAATGCCCTGCGAGAAGTCCCAGCAGCTGTTGGAGATATGCAGAACTTGCAGACGTTTCTGCTG GATGGAAATTTTCTCCAGTCCCTTCCTGCTGAACTGGAGAGCATGCACCAGCTCAGCTATCTGGGTCTTT CCTTTAATGAATTCACTGACATTCCAGAGGTATTGGAGAAGCTGACTGCTGTGGATAAGCTGTGTATGGC TGGGAACTGTGTGGAGACCCTCAGACTACAGGCCTTAAGAAGAATGCCTCATATTAAACATGTGGACCTA AGACTGAACATACTCAGAAAGCTTATGGCAGATGAGGTGGACTTTGTGCAACATGTCACTCAGCTTGACC TGCGAGACAATAAGCTTGGTGATCTAGATGCTATGATCTTCAACAACATAGAAGTTCTGCACTGTGAGAG GAATCAGCTGGTGACATTGAACGTTTGTGGCTATTTCCTAAAGGCACTCTATGCTTCTTCTAATGAACTT GCTCAACTTGATGTCTACCCAGTTCCGAATTATCTCTCTTACATGGATGTCTCAAGAAACTGCCTAGAAA GTGTGCCTGAGTGGGTATGTGAAAGCCGGAAATTAGAAGTTTTGGATATTGGCCATAATCAAATATGTGA ACTTCCTGCCCGCCTGTTTTGTAATAGTAGTCTCCGGAAATTGCTAGCAGGACACAACCGGTTGGCAAGG CTTCCTGAAAGGCTGGAGAGAACTTCTGTGGAGGTCTTGGATGTCCAGCACAACCAGATCACTGAGCTCC CACCAAACCTCCTCATGAAGGCTGACAGCCTGAGATTCCTGAATGCATCTGCAAACAAACTGGAAACCCT GCCTCCAGCCACACTTTCTGAGGAGACGAGCAGTATATTACAGGAGTTGTACCTGACAAACAACTGCCTC ACGGATAAGTGTGTGCCCTTGTTAACAGGGCACCCCCGTCTGAAGATCCTACACATGGCCTATAACCGGC TTCAGAGCTTCCCGGCAAGTAAAATGGCAAAACTGGAGGAACTTGAAGAAATTGATATCAGTGGGAATAA GCTGAAAGCCATCCCCACAACAATCATGAACTGCAGACGCATGCACACCGTGATTGCTCACTCCAATTGT ATCGAAGTCTTTCCTGAAGTTATGCAGCTCCCAGAAGTCAAGTGTGTAGATCTGAGCTGTAATGAGCTGA GTGAAATCACGTTACCAGAAAACCTGCCACCTAAGCTGCAGGAGCTAGACCTGACTGGAAATCCACGCCT TGCCCTGGACCACAAAAGCCTGGAGCTGCTCAATAACATTCGCTGCTTCAAGATTGACCAGCCGTCAGCA GGAGATGCATCAGGAGCCCCAGCAGTATGGAGTCATGGTTACACTGAAGCATCGGGAGTAAAAAACAAGC TGTGTGTCGCAGCCCTGTCTGTAAATAACTTCCGTGACAACCGAGAGGCCCTCTATGGTGTGTTTGATGG AGACCGGAATGTGGAGGTGCCCTACCTTCTCCAGTGCACCATGAGTGACATTTTGGCTGAAGAGCTTCAG AAAACGAAAAATGAAGAAGAATACATGGTCAATACATTCATAGTCATGCAAAGGAAACTGGGAACTGCTG GGCAGAAACTTGGTGGTGCTGCTGTCCTCTGCCACATCAAGCCTGACCCTGTGGACCTGGGAGGATCCTT TACCCTGACCTCTGCTAATGTTGGCAAGTGCCAAACGGTTCTCTGTCGAAATGGGAAGCCACTGTCTCTG TCCAGGTCATACATCATGAGCTGTGAAGAAGAGCGGAAGAGGATTAAGCAGCACAAAGCCATCATCACTG AGGATGGCAAGGTCAATGGAGTGACAGAGTCCACGCGCATCCTGGGCTACACCTTCCTCCATCCCAGTGT GGTGCCTCGCCCCCATGTGCAGTCGGTGCTTCTGACTCCACAGGATGAGTTTTTCATCCTGGGCAGCAAA GGGCTGTGGGACAGCCTATCCATTGACGAGGCTGTGGAAGCTGTACGCAATGTGCCAGATGCTCTGGCTG CTGCTAAGAAGCTCTGCACCCTGGCTCAGAGCTATGGCTGCCATGACAGCATCAGTGCTGTGGTGGTACA GCTAAGTGTCACCGAAGACAGCTTCTGCTGCTGTGAGCTCAGTGCTGGAGGGAGCATGCCACCACCCAGC CCCGGAATCTTCCCACCATCCGTCAACATGGTCATCAAGGACCGACCCTCAGATGGGCTGGGTGTGCCAT CCTCCAGCAGTGGCATGGCATCTGAGATCAGCAGTGAACTGTCTACCTCCGAGATGAGTAGTGAGGTGGG CTCCACAGCCTCTGATGAGCCCCCGTCTGGAGTCCTGAATGAGAGCAGCCCTGCCTACCCCAACGAGCAG CGCTGCATGCTCCACCCTGTCTGCCTGTCTAACTCCTTCCAACGTCAGCTGTCCAGCGCTACTTTCTCCA GCGCGTTCTCTGACAACGGCCTTGACAGTGACGATGAAGAACCCATTGAGGGGGTGTTCAGCAACGGCAG CCGGGTTGAGGTGGAAGTAGACATCCACTGCAGCAGGGCCAAGGAAAAGGAGAGACAGCAGCACCTACTT CAGGTGCCAGCTGAGGCCAGTGATGAGGGCATTGTCATCAGTGCCAATGAGGATGAGTCAGGTCTGTCCA AGAAGGCAGACTTTTCTGCTGTGGGAACCATTGGACGACGCAGGGCTAATGGCTCTGTAGCTCCCCAGGA AAGGAGCCATAATGTGATAGAGGTTGCTGCAGATGCGCCTCTCCGGAAGCCAGGAGGCTATTTTGCAGCC CCTGCTCAGCCAGATCCAGATGATCAGTTTATCATCCCCCCAGAGCTGGAAGAGGAAGTCAAAGAAATCA TGAAACATCACCAGGAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCTGCCACCACCACCCCAGCCGCCACA GCCACAGCCACAGCCACAGCCACAGCCACAGCCACAGCCACAGCGGCACTTCCAAATGGATCACCTGCCA GACTGTTACGATACACCGCTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR226995 representing NM_133821 Red=Cloning site Green=Tags(s) MEPAAAAPAQRLADPTGEDQALAAAAAEGGRCPDPALSAAAPSGGNGGAAREEAPCEAPPGPLPGRAGGT GRRRRRGAPQPAAGGAAPVPAAGGGANSLLLKRGRLKRNLSAAAAASSSSSPSSASSAAGGLPASCSASA SLCTRSLDRKTLLLKHRQLLQLQPSDRDWVRHQLQRGCVHVFDRHMASSYLRPVLCTLDTTAAEVAARLL QLGHKGGGVVKVLGYGPPPAAAPAASDQTLDGEHGRDVEPPPSSGTVGAVRGPARAPPADLPLPGGAWTR CAPRISPAPSDSSPGELFAGGPGSPPRAPRPASDTESFSLSPSAESVSDRLDPYSSGGGGSSSSSEELEA DPAMPHRPGRPAQPRPPSPKTSALLQPKAPTGVDSTGVIAGEGPGDDKAMAAAAPDVPLSTSGRIRETVQ KTSPPSLYVQLHGETTRRLEADEKPLQIQNDYLFQLGFGELWRVQEEGMDSEIGCLIRFYAGKPHSTGSS ERIQLSGMYNVRKGKMQLPVNRWTRRQVILCGTCLIVSSVKDSVSGKMHVLPLIGGKVEEVKKHQHCLAF SSSGPQSQTYYICFDTFTEYLRWLRQVSKVASQRISSVDLSCCSLEHLPANLFYSQDLTHLNLKQNFLRQ TPTLPAARGLGELQRFTKLKSLNLSNNHLGAFPSAVCSIPTLAELNVSCNALREVPAAVGDMQNLQTFLL DGNFLQSLPAELESMHQLSYLGLSFNEFTDIPEVLEKLTAVDKLCMAGNCVETLRLQALRRMPHIKHVDL RLNILRKLMADEVDFVQHVTQLDLRDNKLGDLDAMIFNNIEVLHCERNQLVTLNVCGYFLKALYASSNEL AQLDVYPVPNYLSYMDVSRNCLESVPEWVCESRKLEVLDIGHNQICELPARLFCNSSLRKLLAGHNRLAR LPERLERTSVEVLDVQHNQITELPPNLLMKADSLRFLNASANKLETLPPATLSEETSSILQELYLTNNCL TDKCVPLLTGHPRLKILHMAYNRLQSFPASKMAKLEELEEIDISGNKLKAIPTTIMNCRRMHTVIAHSNC IEVFPEVMQLPEVKCVDLSCNELSEITLPENLPPKLQELDLTGNPRLALDHKSLELLNNIRCFKIDQPSA GDASGAPAVWSHGYTEASGVKNKLCVAALSVNNFRDNREALYGVFDGDRNVEVPYLLQCTMSDILAEELQ KTKNEEEYMVNTFIVMQRKLGTAGQKLGGAAVLCHIKPDPVDLGGSFTLTSANVGKCQTVLCRNGKPLSL SRSYIMSCEEERKRIKQHKAIITEDGKVNGVTESTRILGYTFLHPSVVPRPHVQSVLLTPQDEFFILGSK GLWDSLSIDEAVEAVRNVPDALAAAKKLCTLAQSYGCHDSISAVVVQLSVTEDSFCCCELSAGGSMPPPS PGIFPPSVNMVIKDRPSDGLGVPSSSSGMASEISSELSTSEMSSEVGSTASDEPPSGVLNESSPAYPNEQ RCMLHPVCLSNSFQRQLSSATFSSAFSDNGLDSDDEEPIEGVFSNGSRVEVEVDIHCSRAKEKERQQHLL QVPAEASDEGIVISANEDESGLSKKADFSAVGTIGRRRANGSVAPQERSHNVIEVAADAPLRKPGGYFAA PAQPDPDDQFIIPPELEEEVKEIMKHHQEQQQQQQQQQLPPPPQPPQPQPQPQPQPQPQPQRHFQMDHLP DCYDTPL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_133821
ORF Size	5061 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_133821.3, NP_598582.3
RefSeq Size	6123 bp
RefSeq ORF	5064 bp
Locus ID	98432
UniProt ID	Q8CHE4
MW	182.8 kDa
Gene Summary	Protein phosphatase involved in regulation of Akt and PKC signaling. Mediates dephosphorylation in the C-terminal domain hydrophobic motif of members of the AGC Ser/Thr protein kinase family; specifically acts on 'Ser-473' of AKT2 and AKT3, 'Ser-660' of PRKCB and 'Ser-657' of PRKCA (By similarity). Isoform 2 seems to have a major role in regulating Akt signaling in hippocampal neurons (By similarity). Akt regulates the balance between cell survival and apoptosis through a cascade that primarily alters the function of transcription factors that regulate pro- and antiapoptotic genes. Dephosphorylation of 'Ser-473' of Akt triggers apoptosis and suppression of tumor growth. Dephosphorylation of PRKCA and PRKCB leads to their destabilization and degradation. Dephosphorylates STK4 on 'Thr-387' leading to STK4 activation and apoptosis. Dephosphorylates RPS6KB1 and is involved in regulation of cap-dependent translation. Inhibits cancer cell proliferation and may act as a tumor suppressor. Dephosphorylates RAF1 inhibiting its kinase activity. May act as a negative regulator of K-Ras signaling in membrane rafts (By similarity). Involved in the hippocampus-dependent long-term memory formation (PubMed:17382888). Involved in circadian control by regulating the consolidation of circadian periodicity after resetting (PubMed:20080691). Involved in development and function of regulatory T-cells (PubMed:21498666).[UniProtKB/Swiss-Prot Function]

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