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Pappa (NM_021362) Mouse Tagged ORF Clone
CAT＃: MR227070
- TrueORF®
Pappa (Myc-DDK-tagged) - Mouse pregnancy-associated plasma protein A (Pappa)
ORF Plasmid: tGFP
"NM_021362" in other vectors (4)
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CNY 11,112.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 8430414N03Rik; IGFBP-4ase; PAG1; PAPP-A |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR227070 representing NM_021362
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGGCTCTGGAGTTGGGTGCTGCGCCTGGGGCTGCTGAGCGCCGCGCTGGGCTGCGGGCTGGCCGAGC GCCCCCGCCGGGTCCGAAGAGACCCTCGGGCCGTGCGCCCCCCGCGCCCCGCCGCTGGACCGGCCACCTG CGCCACCCGGGCGGCCCGCGGTCGCCGCGCCTCGCCGCCGCCGCCTCCGGGCGGTGCCTGGGAAGCCGTG CGCGTCCCCCGGCGGCGGCAGCAGCGGGCGGCGAGGGGCGCCGAGGAGCCGAGCCCGCCTAGCCGGGCGC TCTATTTCAGCGGGCGAGGGGAGCAGCTGCGCCTCCGGGCCGACCTGGAGCTACCCCGCGACGCCTTTAC ACTGCAAGTGTGGCTGCGAGCCGAGGGTGGCCAGAAGTCTCCAGCAGTGATCACAGGGCTGTATGACAAA TGTTCTTATACCTCACGTGATCGAGGATGGGTCATGGGCATTCACACCACCAGTGATCAAGGCAACAGAG ATCCACGCTACTTTTTCTCCTTGAAGACAGACCGGGCCAGGAAAGTGACCACCATTGATGCCCATCGCAG CTACCTCCCAGGTCAGTGGGTACATCTAGCTGCTACCTATGATGGGCGGCTGATGAAGCTCTATATGAAT GGTGCCCAGGTGGCAACTTCGGCTGAGCAAGTAGGTGGCATATTCAGCCCACTGACCCAGAAGTGTAAAG TGCTCATGTTGGGGGGCAGTGCTCTGAATCACAACTTCCGGGGCCACATTGAACACTTCAGTCTATGGAA AGTAGCAAGAACCCAGCGAGAGATTGTATCCGACATGGAAACCCGTGGCCTCCACACCCCTCTACCTCAG CTCCTCCTCCAGGAGAACTGGGACAACGTGAAGCGCACTTGGTCCCCCATGAAGGATGGCAACAGCCCCC AGGTGGAATTCAGCAATGCCCACGGCTTCCTGTTGGACACTAATTTGGAGCCCCCTCTTTGTGGGCAGAC ACTGTGTGACAACACAGAAGTCATCTCCAGTTACAATCAGCTCCCAAGTTTTCGGCAGCCCAAGGTGGTC CGCTATCGTGTGGTCAACATCTATGATGATCACCATGAGAATCCAACGGTGAGCTGGCAACAGATTGACT TTCAGCACCAACAGCTGGCTGAGGCCTTTCAACACTACAACATCTCCTGGGAGCTGGAGGTACTGAATAT AAACAGTTCCTCTCTGCGTCACCGCCTCATCCTAGCCAACTGTGACATCAGCAAGATTGGGGATGAAAAA TGTGATCCAGAATGTAACCATACACTGACTGGTCATGATGGTGGAGATTGCCGCCAGCTGCGCTACCCTG CGTTCATGAAGAAGCAGCAGAATGGTGTGTGTGACATGGACTGTAACTACGAAAGGTTTAATTTTGATGG TGGAGAGTGCTGTGACCCAGACATCACTGATGTCACTAAGACATGCTTTGATCCTGACTCTCCACACAGA GCCTACTTGGATGTTAATGAGCTAAAGAACATTCTTAGACTGGACGGATCAACACATCTCAATATTTTCT TTGCAAACTCTTCAGAGGAGGAGTTGGCAGGAGTGGCAACTTGGCCATGGGACAAGGAAGCCCTAATGCA CTTGGGCGGTATTGTCTTGAACCCATCTTTCTATGGCATTCCCGGACACACCCACACCATGATTCATGAG ATTGGGCATAGCCTGGGCCTCTATCACATCTTCCGTGGCATCTCAGAAATCCAGTCCTGCAGTGATCCCT GCATGGAGACAGAGCCTTCATTTGAAACTGGAGACCTCTGCAATGATACCAACCCAGCCCCCAAACACAA GTTTTGTGGAGACCCTGGACCAGGGAATGACACTTGTGGCTTTCATGGCTTCTTCAACACTCCTTACAAC AACTTCATGAGCTACGCAGATGACGACTGTACAGACTCTTTCACGCCCAATCAAGTCTCCAGAATGCACT GTTACCTGGACCTCGTATACCAGAGCTGGCAGCCCTCCAGAAAGCCAGCACCTGTAGCTCTTGCGCCCCA GGTTGTGGGGCACACAATGGACTCTGTGATGCTAGAGTGGTTCCCACCCATCGATGGCCACTTCTTTGAA AGAGAATTGGGATCAGCATGTGACCTTTGCCTAGAAGGGAGAATCCTGGTGCAATATGCTTTCAATGCCT CCTCCCCCATGCCCTGTGGACCGTCAGGACACTGGAGTCCTCGGGAAGCAGAAGGTCACCCAGATGTTGA ACAGCCCTGTAAATCCAGTGTCCGAACCTGGAGTCCAAATTCAGCTGTCAACCCACACACAGTTCCTCCA GCCTGCCCTGAGCCACAAGGCTGCTACCTCGAGCTGGAATTTCGCTACCCTTTGGTCCCTGAGTCTCTGA CCATCTGGGTAACCTTTGTCTCCAGTGATTGGGACTCTAGTGGAGCTGTCAATGACATCAAACTCTTGAC TATCAGTGGAAAGAATATCTCTTTGGGTCCTCAGAATGTTTTCTGTGATATCCCACTTACCATCAGACTC CGGGATGTGGGTGAGGAGGTATATGGCATCCAAATCTATACTCTTGATGAGCACCTGGAGATTGATGCAG CAATGCTGACCTCCACTGTAGACAGTCCACTCTGCCTACAGTGTAAACCTCTGCAGTATAAAGTGCTTCG AGACCCACCTCTGCTAGAAGATGTAGCCTCATTACTCCACCTCAACAGAAGATTCATGGACACGGATCTG AAACTTGGCAGTGTGTACCAGTACCGGATTATCACCATTTCAGGAAATGAAGAGAGCGAGCCATCACCTG CTGCCATATACACCCACGGAAGTGGGTACTGTGGTGATGGCGTTATCCAAAAAGACCAAGGAGAAGAATG TGACGACATGAATAAGGTCAATGGGGATGGCTGCTCCCTTTTCTGCAAGCAAGAAGTTTCCTTCAACTGC ATTGATGAACCCAGCCGGTGCTATTTCCATGATGGGGATGGGATGTGTGAAGAGTTTGAACAAAAAACTA GCATTAAAGACTGTGGTGTCTACACGCCCCAGGGTTTCCTGGATCAGTGGGCATCCAATGCTTCAGTATC TCATCAAGACCAGCAGTGCCCAGGTTGGGTTGTCATTGGGCAGCCAGCGGCATCTCAGGTGTGTCGAACC AAGGTGATAGATCTCAGTGAAGGCATTTCCCAGCATGCTTGGTATCCTTGCACCATTACTTACCCATACT ACCATCTGCCTCAGACCACATTCTGGCTCCAGACATATTTCTCTCAGCCAATGGTTGCTGCAGCTGTAAT TATTCACCTGGTGACTGATGGGACATACTATGGGGACCAAAAGCAAGAGACCATCAGTGTGCAGTTGCTT GATACCAAAGATCAAAGCCATGATCTAGGCCTCCATGTCTTGAGCTGCAGAAACAATCCCCTGATTATCC CTGTGGTCCATGACCTCAGCCAGCCCTTCTACCACAGCCAGGCGGTACATGTGAGCTTCAGTTCGCCCCT GGTCGCCATCTCGGGGGTGGCCCTCCGCTCTTTCGACAACTTTGACCCCGTCACCCTGAGCAGCTGCCAG AGAGGAGAGACCTACAGCCCTGCTGAGCAGAGCTGTGTGCATTTTGCCTGTCAAGCTGCCGACTGCCCAG AACTGGCCGTGGGGAATGCTTCTCTCAACTGTTCCAGCAACCACCACTACCATGGTGCCCAGTGCACTGT GAGCTGCCAGACAGGTTATGTGCTGCAGATACAGCGGGACGATGAGCTAATCAAGAGCCAGGTAGGGCCA AGCATCACAGTGACATGTACCGAGGGCAAATGGAACAAGCAGGTGGCATGTGAGCCGGTGGACTGTGGTA TCCCAGATCACCATCACGTCTATGCTGCCTCCTTCTCCTGTCCAGAGGGTACCACCTTTGGTAGAAGATG TTCTTTTCAGTGTCGCCACCCTGCCCAGCTGAAAGGCAACAACAGCTTTCTGACCTGTATGGAAGATGGA CTGTGGTCCTTCCCAGAGGCCTTGTGTGAGCTCATGTGCCTCGCCCCACCCCCAGTTCCCAATGCGGACC TACAGACAGCCCGGTGTCGAGAGAACAAGCACAAGGTGGGCTCCTTCTGCAAGTACAAGTGTAAACCTGG ATACCACGTGCCTGGCTCATCTCGGAAGTCCAAGAAACGGGCTTTCAAGACTCAATGTACTCAAGATGGC AGCTGGCAAGAGGGAACTTGTGTGCCGGTGACTTGTGACCCACCTCCACCCAAATTCCATGGGCTCTATC AATGCACTAATGGCTTCCAGTTCAATAGTGAGTGCAGGATCAAGTGTGAAGACAGTGATGCCTCCCAGGG CCGTGGGAGCAATATCATTCACTGCCGGAAAGATGGCACTTGGAGTGGTTCCTTCCACGTCTGCCGAGAG ATGCAAGGCCAGTGCTCAGCCCCAAACCAACTCAACAGTAACCTCAAATTGCAGTGTCCTGATGGCTATG CAATAGGGTCAGAGTGTGCCATCTCGTGCCTGGACCATAACAGCGAGTCCATCATCCTTCCCGTTAACTT GACAGTGCGTGACATACCCCATTGGATGAACCCCACACGAGTACAGAGGATTGTCTGCACTGCTGGTCTC CAGTGGTATCCCCACCCTGCTCTGATCCACTGTGTCAAAGGCTGTGAGCCATTCATGGGAGACAACTACT GTGATGCCATCAACAATCGAGCCTTCTGCAACTATGATGGTGGGGACTGCTGCACCTCCACAGTAAAGAC CAAAAAGGTCACTCCCTTTCCTATGTCCTGTGACCTACAAAATGACTGCGCCTGTCGAGACCCTGAGGCC CAAGAACACAACCGGAAAGATCTTCGGGGATATAGCCATGGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR227070 representing NM_021362
Red=Cloning site Green=Tags(s) MRLWSWVLRLGLLSAALGCGLAERPRRVRRDPRAVRPPRPAAGPATCATRAARGRRASPPPPPGGAWEAV RVPRRRQQRAARGAEEPSPPSRALYFSGRGEQLRLRADLELPRDAFTLQVWLRAEGGQKSPAVITGLYDK CSYTSRDRGWVMGIHTTSDQGNRDPRYFFSLKTDRARKVTTIDAHRSYLPGQWVHLAATYDGRLMKLYMN GAQVATSAEQVGGIFSPLTQKCKVLMLGGSALNHNFRGHIEHFSLWKVARTQREIVSDMETRGLHTPLPQ LLLQENWDNVKRTWSPMKDGNSPQVEFSNAHGFLLDTNLEPPLCGQTLCDNTEVISSYNQLPSFRQPKVV RYRVVNIYDDHHENPTVSWQQIDFQHQQLAEAFQHYNISWELEVLNINSSSLRHRLILANCDISKIGDEK CDPECNHTLTGHDGGDCRQLRYPAFMKKQQNGVCDMDCNYERFNFDGGECCDPDITDVTKTCFDPDSPHR AYLDVNELKNILRLDGSTHLNIFFANSSEEELAGVATWPWDKEALMHLGGIVLNPSFYGIPGHTHTMIHE IGHSLGLYHIFRGISEIQSCSDPCMETEPSFETGDLCNDTNPAPKHKFCGDPGPGNDTCGFHGFFNTPYN NFMSYADDDCTDSFTPNQVSRMHCYLDLVYQSWQPSRKPAPVALAPQVVGHTMDSVMLEWFPPIDGHFFE RELGSACDLCLEGRILVQYAFNASSPMPCGPSGHWSPREAEGHPDVEQPCKSSVRTWSPNSAVNPHTVPP ACPEPQGCYLELEFRYPLVPESLTIWVTFVSSDWDSSGAVNDIKLLTISGKNISLGPQNVFCDIPLTIRL RDVGEEVYGIQIYTLDEHLEIDAAMLTSTVDSPLCLQCKPLQYKVLRDPPLLEDVASLLHLNRRFMDTDL KLGSVYQYRIITISGNEESEPSPAAIYTHGSGYCGDGVIQKDQGEECDDMNKVNGDGCSLFCKQEVSFNC IDEPSRCYFHDGDGMCEEFEQKTSIKDCGVYTPQGFLDQWASNASVSHQDQQCPGWVVIGQPAASQVCRT KVIDLSEGISQHAWYPCTITYPYYHLPQTTFWLQTYFSQPMVAAAVIIHLVTDGTYYGDQKQETISVQLL DTKDQSHDLGLHVLSCRNNPLIIPVVHDLSQPFYHSQAVHVSFSSPLVAISGVALRSFDNFDPVTLSSCQ RGETYSPAEQSCVHFACQAADCPELAVGNASLNCSSNHHYHGAQCTVSCQTGYVLQIQRDDELIKSQVGP SITVTCTEGKWNKQVACEPVDCGIPDHHHVYAASFSCPEGTTFGRRCSFQCRHPAQLKGNNSFLTCMEDG LWSFPEALCELMCLAPPPVPNADLQTARCRENKHKVGSFCKYKCKPGYHVPGSSRKSKKRAFKTQCTQDG SWQEGTCVPVTCDPPPPKFHGLYQCTNGFQFNSECRIKCEDSDASQGRGSNIIHCRKDGTWSGSFHVCRE MQGQCSAPNQLNSNLKLQCPDGYAIGSECAISCLDHNSESIILPVNLTVRDIPHWMNPTRVQRIVCTAGL QWYPHPALIHCVKGCEPFMGDNYCDAINNRAFCNYDGGDCCTSTVKTKKVTPFPMSCDLQNDCACRDPEA QEHNRKDLRGYSHG TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_021362 |
| ORF Size | 4872 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_021362.1, NP_067337.1 |
| RefSeq Size | 11027 bp |
| RefSeq ORF | 4875 bp |
| Locus ID | 18491 |
| UniProt ID | Q8R4K8 |
| MW | 181.8 kDa |
| Gene Summary | Metalloproteinase which specifically cleaves IGFBP-4 and IGFBP-5, resulting in release of bound IGF. Cleavage of IGFBP-4 is dramatically enhanced by the presence of IGF, whereas cleavage of IGFBP-5 is slightly inhibited by the presence of IGF. Isoform 2 cleaves IGFBP-4 very slowly compared to PAPP-A, but its ability to cleave IGFBP-5 is unaffected.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC224767 | Pappa (untagged) - Mouse pregnancy-associated plasma protein A (Pappa), (10ug) |
CNY 20,810.00 |
|
| MG227070 | Pappa (tGFP-tagged) - Mouse pregnancy-associated plasma protein A (Pappa), (10ug) |
CNY 13,300.00 |
|
| MR227070L3 | Lenti ORF clone of Pappa (Myc-DDK-tagged) - Mouse pregnancy-associated plasma protein A (Pappa) |
CNY 13,970.00 |
|
| MR227070L4 | Lenti ORF clone of Pappa (mGFP-tagged) - Mouse pregnancy-associated plasma protein A (Pappa) |
CNY 13,970.00 |
