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Esr1 (NM_007956) Mouse Tagged ORF Clone
CAT＃: MR227304
- TrueORF®
Esr1 (Myc-DDK-tagged) - Mouse estrogen receptor 1 (alpha) (Esr1)
ORF Plasmid: tGFP
"NM_007956" in other vectors (6)
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CNY 6,688.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | E; ER; ER-; ER-alpha; ERa; ERalpha; ER[; Es; ESR; Estr; Estra; Nr; Nr3a1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR227304 representing NM_007956
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCATGACCCTTCACACCAAAGCCTCGGGAATGGCCTTGCTGCACCAGATCCAAGGGAACGAGCTGG AGCCCCTCAACCGCCCGCAGCTCAAGATGCCCATGGAGAGGGCCCTGGGCGAGGTATACGTGGACAACAG CAAGCCCACTGTGTTCAACTACCCCGAGGGCGCCGCCTACGAGTTCAACGCCGCCGCCGCCGCCGCCGCC GCCGCCTCGGCGCCGGTCTACGGCCAGTCGGGCATCGCCTACGGCCCCGGGTCGGAGGCGGCCGCCTTCA GTGCCAACAGCCTGGGGGCTTTCCCCCAGCTCAACAGCGTGTCGCCTAGCCCGCTGATGCTGCTGCACCC GCCGCCGCAGCTGTCTCCTTTCCTGCACCCGCACGGCCAGCAGGTGCCCTACTACCTGGAGAACGAGCCC AGCGCCTACGCCGTGCGCGACACCGGCCCTCCCGCCTTCTACAGGTCTAATTCTGACAATCGACGCCAGA ATGGCCGAGAGAGACTGTCCAGCAGTAACGAGAAAGGAAACATGATCATGGAGTCTGCCAAGGAGACTCG CTACTGTGCCGTGTGCAATGACTATGCCTCTGGCTACCATTATGGGGTCTGGTCCTGCGAAGGCTGCAAG GCTTTCTTTAAGAGAAGCATTCAAGGACACAATGACTACATGTGTCCAGCTACAAACCAATGCACCATTG ACAAGAACCGGAGGAAGAGTTGCCAGGCCTGTCGGCTGCGCAAGTGTTACGAAGTGGGCATGATGAAAGG CGGCATACGGAAAGACCGCCGAGGAGGGAGAATGTTGAAGCACAAGCGTCAGAGAGATGACTTGGAAGGC CGAAATGAAATGGGTGCTTCAGGAGACATGAGGGCTGCCAACCTTTGGCCAAGCCCTCTTGTGATTAAGC ACACTAAGAAGAATAGCCCTGCCTTGTCCTTGACAGCTGACCAGATGGTCAGTGCCTTGTTGGATGCTGA ACCGCCCATGATCTATTCTGAATATGATCCTTCTAGACCCTTCAGTGAAGCCTCAATGATGGGCTTATTG ACCAACCTAGCAGATAGGGAGCTGGTTCATATGATCAACTGGGCAAAGAGAGTGCCAGGCTTTGGGGACT TGAATCTCCATGATCAGGTCCACCTTCTCGAGTGTGCCTGGCTGGAGATTCTGATGATTGGTCTCGTCTG GCGCTCCATGGAACACCCGGGGAAGCTCCTGTTTGCTCCTAACTTGCTCCTGGACAGGAATCAAGGTAAA TGTGTGGAAGGCATGGTGGAGATCTTTGACATGTTGCTGGCTACGTCAAGTCGGTTCCGCATGATGAACC TGCAGGGAGAAGAGTTTGTGTGCCTCAAATCCATCATTTTGCTTAATTCCGGAGTGTACACGTTTCTGTC CAGCACCTTGAAGTCTCTGGAAGAGAAGGACCACATCCACCGTGTCCTGGACAAGATCACAGACACTTTG ATCCACCTGATGGCCAAAGCTGGCCTGACTCTGCAGCAGCAGCATCGCCGCCTAGCTCAGCTCCTTCTCA TTCTTTCCCATATCCGGCACATGAGTAACAAAGGCATGGAGCATCTCTACAACATGAAATGCAAGAACGT TGTGCCCCTCTATGACCTGCTCCTGGAGATGTTGGATGCCCACCGCCTTCATGCCCCAGCCAGTCGCATG GGAGTGCCCCCAGAGGAGCCCAGCCAGACCCAGCTGGCCACCACCAGCTCCACTTCAGCACATTCCTTAC AAACCTACTACATACCCCCGGAAGCAGAGGGCTTCCCCAACACGATC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR227304 representing NM_007956
Red=Cloning site Green=Tags(s) MTMTLHTKASGMALLHQIQGNELEPLNRPQLKMPMERALGEVYVDNSKPTVFNYPEGAAYEFNAAAAAAA AASAPVYGQSGIAYGPGSEAAAFSANSLGAFPQLNSVSPSPLMLLHPPPQLSPFLHPHGQQVPYYLENEP SAYAVRDTGPPAFYRSNSDNRRQNGRERLSSSNEKGNMIMESAKETRYCAVCNDYASGYHYGVWSCEGCK AFFKRSIQGHNDYMCPATNQCTIDKNRRKSCQACRLRKCYEVGMMKGGIRKDRRGGRMLKHKRQRDDLEG RNEMGASGDMRAANLWPSPLVIKHTKKNSPALSLTADQMVSALLDAEPPMIYSEYDPSRPFSEASMMGLL TNLADRELVHMINWAKRVPGFGDLNLHDQVHLLECAWLEILMIGLVWRSMEHPGKLLFAPNLLLDRNQGK CVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTL IHLMAKAGLTLQQQHRRLAQLLLILSHIRHMSNKGMEHLYNMKCKNVVPLYDLLLEMLDAHRLHAPASRM GVPPEEPSQTQLATTSSTSAHSLQTYYIPPEAEGFPNTI TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_007956 |
| ORF Size | 1797 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_007956.5 |
| RefSeq Size | 4319 bp |
| RefSeq ORF | 1800 bp |
| Locus ID | 13982 |
| UniProt ID | P19785 |
| MW | 67.4 kDa |
| Gene Summary | This gene encodes an estrogen receptor, a member of the nuclear hormone family of intracellular receptors. The encoded protein, activated by the sex hormone estrogen, is a transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Similar genes in human have been implicated in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC219495 | Esr1 (untagged) - Mouse estrogen receptor 1 (alpha) (Esr1), (10ug) |
CNY 6,696.00 |
|
| MG227304 | Esr1 (tGFP-tagged) - Mouse estrogen receptor 1 (alpha) (Esr1), (10ug) |
CNY 8,288.00 |
|
| MR227304L1 | Lenti ORF clone of Esr1 (Myc-DDK-tagged) - Mouse estrogen receptor 1 (alpha) (Esr1) |
CNY 7,890.00 |
|
| MR227304L2 | Lenti ORF clone of Esr1 (mGFP-tagged) - Mouse estrogen receptor 1 (alpha) (Esr1) |
CNY 9,088.00 |
|
| MR227304L3 | Lenti ORF clone of Esr1 (Myc-DDK-tagged) - Mouse estrogen receptor 1 (alpha) (Esr1) |
CNY 7,890.00 |
|
| MR227304L4 | Lenti ORF clone of Esr1 (mGFP-tagged) - Mouse estrogen receptor 1 (alpha) (Esr1) |
CNY 7,890.00 |
