Cdh3 (NM_007665) Mouse Tagged ORF Clone
CAT＃: MR227345
- TrueORF®
Cdh3 (Myc-DDK-tagged) - Mouse cadherin 3 (Cdh3), transcript variant 2
ORF Plasmid: tGFP
"NM_007665" in other vectors (4)
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CNY 6016.00
CNY 7220.00
| Cited in 1 publication. |
Product images
CNY 6840.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AI385538; Ca; Cadp; Cdhp; P-cad; P-cadherin; Pc; Pcad |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR227345 representing NM_007665
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCTTCTTAGTGGGCCTCACGCCTTCCTGCTCCTCCTGCTCCAGGTTTGCTGGCTACGCAGCGTGG TCTCCGAGCCCTACCGAGCGGGCTTCATCGGGGAGGCTGGAGTGACCTTGGAGGTGGAAGGAACTGACCT GGAGCCGAGCCAAGTTCTGGGGAAAGCCTTGGCTGGACAGGGCATGCACCATGCAGACAATGGAGACATC ATTATGCTGACTAGGGGGACAGTTCAGGGAGGGAAGGATGCGATGCACTCCCCACCCACCCGCATCTTAA GGAGACGAAAGAGAGAGTGGGTGATGCCACCAATATTCGTCCCCGAGAATGGCAAGGGTCCCTTCCCTCA GAGGCTGAATCAGCTCAAATCTAATAAGGACAGAGGCACCAAGATTTTCTACAGCATCACAGGGCCTGGC GCAGACAGTCCCCCCGAAGGAGTCTTCACCATAGAGAAGGAGTCGGGCTGGCTGTTGTTGCATATGCCAC TGGACAGGGAGAAGATTGTCAAGTACGAGCTTTATGGCCACGCTGTATCTGAGAATGGTGCCTCTGTAGA GGAGCCCATGAACATATCCATCATTGTGACAGACCAGAATGACAACAAGCCCAAGTTCACTCAAGACACC TTCAGAGGGAGTGTTCTGGAGGGAGTAATGCCTGGCACTTCTGTGATGCAGGTGACAGCCACAGATGAGG ACGATGCTGTCAACACTTACAATGGGGTGGTGGCTTACTCCATCCATAGCCAAGAGCCGAAGGAGCCACA CGACCTCATGTTCACCATCCATAAAAGCACGGGAACCATTAGCGTCATATCCAGTGGCCTGGACCGAGAG AAAGTCCCTGAGTACAGACTGACCGTCCAGGCCACAGACATGGATGGAGAGGGCTCTACCACGACGGCAG AGGCCGTTGTGCAAATCCTTGATGCCAACGATAACGCTCCGGAGTTTGAGCCGCAGAAGTATGAGGCTTG GGTGCCTGAGAACGAAGTGGGCCATGAGGTACAGAGGCTGACAGTGACTGATCTCGATGTCCCCAACTCG CCAGCGTGGCGTGCCACCTACCACATCGTGGGAGGTGATGATGGGGACCATTTCACCATCACCACTCACC CAGAGACCAACCAAGGCGTCCTGACAACCAAGAAGGGTTTGGATTTTGAGGCTCAGGACCAACACACCCT GTATGTAGAAGTGACCAACGAGGCTCCCTTTGCAGTGAAGCTCCCGACAGCCACTGCCACCGTGGTGGTC CATGTGAAAGATGTCAACGAAGCCCCTGTGTTTGTTCCACCTTCCAAGGTCATTGAGGCCCAGGAAGGCA TCTCTATTGGGGAACTGGTCTGCATCTATACCGCACAGGACCCAGACAAGGAGGACCAGAAGATCAGCTA CACCATCTCGAGAGATCCAGCCAACTGGCTTGCTGTGGACCCAGACAGTGGTCAGATAACTGCCGCAGGC ATCTTGGATCGTGAGGACGAGCAGTTTGTGAAAAACAATGTCTACGAAGTCATGGTTTTGGCCACAGACA GTGGAAACCCTCCCACCACCGGCACTGGGACCCTCCTGCTTACACTTACTGACATCAACGACCATGGCCC GATCCCTGAACCCAGGCAGATCATCATCTGTAACCAAAGCCCTGTGCCTCAAGTGCTGAACATCACTGAC AAGGACCTGTCCCCCAACTCCTCCCCTTTCCAGGCCCAGCTAACACATGACTCAGATATCTACTGGATGG CAGAAGTCAGCGAGAAAGGAGACACCGTGGCCTTGTCCCTGAAGAAGTTCCTGAAACAAGACACGTATGA CTTGCATCTTTCTTTGTCTGACCATGGCAACAGGGAACAGCTAACCATGATCAGGGCCACTGTGTGTGAC TGCCATGGCCAAGTGTTCAATGACTGCCCCAGACCCTGGAAGGGTGGTTTCATCCTCCCCATCCTGGGTG CTGTCCTGGCACTGCTGACCCTTCTACTGGCACTCCTCCTGTTGGTGAGGAAGAAGAGGAAGGTCAAAGA GCCCCTTCTGCTCCCAGAAGATGACACGCGAGACAATGTCTTCTATTATGGAGAAGAGGGTGGTGGTGAA GAGGACCAGGACTATGACATCACCCAACTCCACCGGGGACTGGAGGCCAGGCCTGAGGTGGTTCTCCGAA ACGATGTAGTGCCAACCTTCATCCCCACCCCCATGTACCGACCCCGGCCCGCCAACCCAGATGAAATCGG GAACTTCATCATCGAGAACCTGAAGGCTGCCAACACTGACCCTACTGCCCCGCCCTACGACTCCCTGCTG GTTTTTGACTACGAGGGCAGCGGCTCTGATGCCGCCTCCCTGAGCTCCCTCACCACCTCCGCCTCCGACC AGGATCAGGACTACAACTACCTTAACGAGTGGGGAAGTCGATTCAAGAAACTGGCGGACATGTATGGTGG CGGTGAGGATGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR227345 representing NM_007665
Red=Cloning site Green=Tags(s) MELLSGPHAFLLLLLQVCWLRSVVSEPYRAGFIGEAGVTLEVEGTDLEPSQVLGKALAGQGMHHADNGDI IMLTRGTVQGGKDAMHSPPTRILRRRKREWVMPPIFVPENGKGPFPQRLNQLKSNKDRGTKIFYSITGPG ADSPPEGVFTIEKESGWLLLHMPLDREKIVKYELYGHAVSENGASVEEPMNISIIVTDQNDNKPKFTQDT FRGSVLEGVMPGTSVMQVTATDEDDAVNTYNGVVAYSIHSQEPKEPHDLMFTIHKSTGTISVISSGLDRE KVPEYRLTVQATDMDGEGSTTTAEAVVQILDANDNAPEFEPQKYEAWVPENEVGHEVQRLTVTDLDVPNS PAWRATYHIVGGDDGDHFTITTHPETNQGVLTTKKGLDFEAQDQHTLYVEVTNEAPFAVKLPTATATVVV HVKDVNEAPVFVPPSKVIEAQEGISIGELVCIYTAQDPDKEDQKISYTISRDPANWLAVDPDSGQITAAG ILDREDEQFVKNNVYEVMVLATDSGNPPTTGTGTLLLTLTDINDHGPIPEPRQIIICNQSPVPQVLNITD KDLSPNSSPFQAQLTHDSDIYWMAEVSEKGDTVALSLKKFLKQDTYDLHLSLSDHGNREQLTMIRATVCD CHGQVFNDCPRPWKGGFILPILGAVLALLTLLLALLLLVRKKRKVKEPLLLPEDDTRDNVFYYGEEGGGE EDQDYDITQLHRGLEARPEVVLRNDVVPTFIPTPMYRPRPANPDEIGNFIIENLKAANTDPTAPPYDSLL VFDYEGSGSDAASLSSLTTSASDQDQDYNYLNEWGSRFKKLADMYGGGEDD TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
| ACCN | NM_007665 |
| ORF Size | 2463 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_007665.3, NP_031691.1 |
| RefSeq Size | 4034 bp |
| RefSeq ORF | 2466 bp |
| Locus ID | 12560 |
| MW | 91 kDa |
| Gene Summary | This gene encodes a calcium-dependent cell-cell adhesion protein containing five cadherin domains. The encoded protein plays a role in epithelial outgrowth, such as that which occurs during the development of hair follicles and limb buds. Loss of function of the related gene in humans results in ectodermal dysplasia, ectrodactyly, and macular dystrophy and congential hypotrichosis with juvenile macular dystrophy. This gene is located in the vicinity of similar cadherin genes on chromosome 8. The proprotein is further cleaved into a functional chain. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2013] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Stem cell-derived synthetic embryos self-assemble by exploiting cadherin codes and cortical tension.
,null,
Nature cell biology
,PubMed ID 36100738
[Cdh3]
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Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC221847 | Cdh3 (untagged) - Mouse cadherin 3 (Cdh3), transcript variant 2, (10ug) |
CNY 10550.00 |
|
| MG227345 | Cdh3 (tGFP-tagged) - Mouse cadherin 3 (Cdh3) transcript variant 2, (10ug) |
CNY 7980.00 |
|
| MR227345L3 | Lenti ORF clone of Cdh3 (Myc-DDK-tagged) - Mouse cadherin 3 (Cdh3), transcript variant 2 |
CNY 9120.00 |
|
| MR227345L4 | Lenti ORF clone of Cdh3 (mGFP-tagged) - Mouse cadherin 3 (Cdh3), transcript variant 2 |
CNY 9120.00 |
