United States
Germany
Japan
United Kingdom
China
Nlrc4 (NM_001033367) Mouse Tagged ORF Clone
CAT＃: MR227430
- TrueORF®
Nlrc4 (Myc-DDK-tagged) - Mouse NLR family, CARD domain containing 4 (Nlrc4)
ORF Plasmid: tGFP
"NM_001033367" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 7,128.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 9530011P19Rik; Card12; CLAN; CLAN1; CLANA; CLANB; CLANC; CLAND; IPAF |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR227430 representing NM_001033367
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACTTTATAAGGAACAACAGACGAGCCCTTATTCAAAGGATGGGCTTAACAGTTACCAAGCAAATCT GCGATGACCTCTTTGCATTGAACGTTCTCAACAATCAAGAAGCTAATGTCATTTACTGTGAGCCCTTGGA GCAGGAAGCCGCCCGAAAGATCATCCATATGACTATGCAGAAGGGCTCAGCGGCCTGCAACCTCTTTCTT AAGAGTCTTGAAAACTGGGACTATTTTGTGTATCAGGACTTAACTGGACAAAATCTTTCTTATCAGGTCA CAGAAGAAGACCTGAATGTTTTGGCCCAGAATTTAAAGGACTTGTACAACAGCCCTGCTTTTCTGAACTT CTACCCCCTGGGTGAAGATATCGACATAATTTTTAATCTGGAGAAAACCTTCACAGAACCTATCATGTGG AAGAAGGACCATCGTCATCACCGTGTGGAGCAGCTGACTTTGGGCAGCCTGCTCGAGGCTCTGAAGAGCC CCTGCCTGATTGAAGGCGAGTCTGGCAAAGGGAAGTCCACCCTGCTGCAGAGAATCGCTATGCTCTGGGC CTCTGGGGGCTGCAGGGCTCTGAAGGGGTTCAGATTAGTCTTCTTCATCCACCTGAGAAGCGCCAGGGGG GGACTATTCGAAACACTGTACGATCAGCTCCTGAACATACCCGACTTCATCAGCAAGCCGACCTTCAAGG CTCTGCTGCTGAAGCTACACAAGGAGGTCCTCTTTCTTCTCGATGGTTACAATGAATTCCATCCCCAGAA CTGCCCAGAAATTGAAGCCCTGATAAAGGAAAACCATCGCTTCAAGAACATGGTCATTGTCACCACCACC ACGGAGTGCCTGAGGCATATCAGACATGTTGGCGCCCTGACTGCGGAGGTGGGAGATATGACCGAAGACA GTGCCAAAGATCTCATCGAGGCAGTGCTGGTACCTGATCAGGTTGAACGCCTGTGGGCCCAAATCCAGGA GTCCAGGTGCCTGAGAAATCTGATGAAGACCCCTCTCTTCGTGGTGATCACCTGTGCAATTCAGATGGGC AGACAGGAATTCCAAGCTCACACCCAAACCATGCTGTTCCAAACCTTCTACGACCTCCTGATACAGAAAA ACAGCCACAGATATAGAGGTGGAGCTTCAGGTGATTTTGCCAGGAGCCTAGACTACTGTGGAGACCTGGC CCTAGAAGGTGTGTTCGCCCACAAATTTGATTTTGAACCCGAGCATGGGTCCAGCATGAACGAGGACGTC CTGGTGACAATAGGGCTCCTCTGTAAGTACACAGCTCAGAGGCTGAAGCCCACGTATAAATTCTTTCATA AATCATTTCAGGAGTACACGGCAGGTCGGAGACTCAGCAGTTTGCTGACGTCCAAAGAGCCAGAGGAGGT GAGCAAAGGGAACAGCTACTTAAACAAAATGGTTTCCATCTCTGACATCACATCCCTATATGGCAATCTG CTCCTCTACACGTGTGGGTCGTCCACAGAAGCAACCAGGGCGGTCATGAGGCACCTTGCAATGGTTTATC AGCACGGCAGCCTACAAGGACTTTCAGTCACCAAGAGGCCTCTCTGGAGGCAGGAATCAATCCAGAGTCT GAGAAATACCACTGAGCAAGATGTTCTGAAAGCCATCAATGTAAATTCCTTCGTAGAGTGTGGCATCAAT TTGTTCTCAGAGAGTATGTCTAAATCAGACCTGAGCCAAGAATTTGAAGCTTTCTTTCAAGGTAAAAGTT TATACATCAACTCAGAGAACATCCCTGACTATTTATTTGACTTCTTTGAATACTTGCCTAATTGTGCAAG CGCATTGGACTTCGTGAAGTTGGATTTCTATGAAAGAGCTACAGAGTCACAGGACAAGGCAGAAGAGAAT GTCCCTGGAGTTCACACAGAAGGGCCCTCAGAAACCTACATTCCCCCCAGGGCTGTGTCTTTGTTCTTCA ACTGGAAGCAGGAATTCAAGACTCTAGAGGTCACACTCCGAGATATTAACAAGTTGAATAAGCAAGATAT CAAATATCTGGGGAAGATATTCAGCTCTGCCACCAACCTCCGGCTGCATATCAAGAGATGTGCAGCCATG GCTGGAAGACTCAGCTCAGTCCTCAGAACCTGCAAGAACATGCATACCCTCATGGTGGAAGCCAGTCCCC TCACCACGGATGACGAACAGTACATCACATCTGTGACAGGCCTCCAGAACTTAAGTATTCACCGCTTGCA CACTCAACAGCTGCCAGGTGGTCTGATTGACAGCTTGGGTAATCTGAAGAACCTCGAGAGACTCATACTG GATGACATCAGGATGAACGAGGAAGATGCTAAAAACCTAGCCGAAGGCCTACGAAGCCTGAAGAAGATGC GTTTACTCCATTTGACTCATTTGTCTGACATTGGGGAGGGGATGGACTACATAGTCAAGTCTCTCTCAGA AGAATCCTGTGATCTCCAAGAGATGAAGTTGGTGGCCTGCTGTCTGACTGCAAACTCTGTGAAAGTTCTA GCACAGAATCTTCACAATTTGATCAAGCTGAGCATTCTTGATATATCAGAAAATTACCTGGAAAAGGATG GGAATGAAGCTCTACAGGAACTGATCGGCAGGCTTGGCGTTCTGGGAGAGCTCACTACATTGATGCTGCC TTGGTGCTGGGATGTGCACACCAGCCTGCCCAAGCTGTTGAAGCAGTTGGAGGGGACCCCAGGACTTGCC AAACTTGGATTGAAAAACTGGAGACTCAGAGACGAAGAGATTAAAAGTTTAGGTGAATTTCTGGAGATGA ATCCTCTGAGAGACTTGCAGCAGTTGGATTTAGCGGGGCACTGTGTGAGCAGTGACGGATGGCTTTACTT CATGAATGTGTTTGAGAATCTGAAGCAGTTAGTGTTTTTTGACTTTAGCACTGAGGAGTTCTTACCGGAT GCAGCACTGGTGAGGAAACTTAGTCAAGTGTTATCCAAGTTAACTCTTCTGCAAGAGGTAAAGCTCACGG GCTGGGAGTTTGATGACTATGATATTAGCGCTATTAAAGGCACCTTTAAACTAGTGACTGCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR227430 representing NM_001033367
Red=Cloning site Green=Tags(s) MNFIRNNRRALIQRMGLTVTKQICDDLFALNVLNNQEANVIYCEPLEQEAARKIIHMTMQKGSAACNLFL KSLENWDYFVYQDLTGQNLSYQVTEEDLNVLAQNLKDLYNSPAFLNFYPLGEDIDIIFNLEKTFTEPIMW KKDHRHHRVEQLTLGSLLEALKSPCLIEGESGKGKSTLLQRIAMLWASGGCRALKGFRLVFFIHLRSARG GLFETLYDQLLNIPDFISKPTFKALLLKLHKEVLFLLDGYNEFHPQNCPEIEALIKENHRFKNMVIVTTT TECLRHIRHVGALTAEVGDMTEDSAKDLIEAVLVPDQVERLWAQIQESRCLRNLMKTPLFVVITCAIQMG RQEFQAHTQTMLFQTFYDLLIQKNSHRYRGGASGDFARSLDYCGDLALEGVFAHKFDFEPEHGSSMNEDV LVTIGLLCKYTAQRLKPTYKFFHKSFQEYTAGRRLSSLLTSKEPEEVSKGNSYLNKMVSISDITSLYGNL LLYTCGSSTEATRAVMRHLAMVYQHGSLQGLSVTKRPLWRQESIQSLRNTTEQDVLKAINVNSFVECGIN LFSESMSKSDLSQEFEAFFQGKSLYINSENIPDYLFDFFEYLPNCASALDFVKLDFYERATESQDKAEEN VPGVHTEGPSETYIPPRAVSLFFNWKQEFKTLEVTLRDINKLNKQDIKYLGKIFSSATNLRLHIKRCAAM AGRLSSVLRTCKNMHTLMVEASPLTTDDEQYITSVTGLQNLSIHRLHTQQLPGGLIDSLGNLKNLERLIL DDIRMNEEDAKNLAEGLRSLKKMRLLHLTHLSDIGEGMDYIVKSLSEESCDLQEMKLVACCLTANSVKVL AQNLHNLIKLSILDISENYLEKDGNEALQELIGRLGVLGELTTLMLPWCWDVHTSLPKLLKQLEGTPGLA KLGLKNWRLRDEEIKSLGEFLEMNPLRDLQQLDLAGHCVSSDGWLYFMNVFENLKQLVFFDFSTEEFLPD AALVRKLSQVLSKLTLLQEVKLTGWEFDDYDISAIKGTFKLVTA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001033367 |
| ORF Size | 3072 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001033367.3, NP_001028539.1 |
| RefSeq Size | 3838 bp |
| RefSeq ORF | 3075 bp |
| Locus ID | 268973 |
| UniProt ID | Q3UP24 |
| MW | 117.2 kDa |
| Gene Summary | Key component of inflammasomes that indirectly senses specific proteins from pathogenic bacteria and fungi and responds by assembling an inflammasome complex that promotes caspase-1 activation, cytokine production and macrophage pyroptosis. The NLRC4 inflammasome is activated as part of the innate immune response to a range of intracellular bacteria. It senses pathogenic proteins of the type III secretion system (T3SS) and type IV secretion system (T4SS) such as flagellin and PrgJ-like rod proteins via the Naip proteins (Naip1, Naip2 or Naip5): specific Naip proteins recognize and bind pathogenic proteins, driving assembly and activation of the NLRC4 inflammasome. The NLRC4 inflammasome senses Gram-negative bacteria such as L.pneumophila and P.aeruginosa, enteric pathogens S.typhimurium (Salmonella) and S.flexneri and fungal pathogen C.albicans. In intestine, the NLRC4 inflammasome is able to discriminate between commensal and pathogenic bacteria and specifically drives production of interleukin-1 beta (IL1B) in response to infection by Salmonella or P.aeruginosa. In case of L.pneumophila infection the inflammasome acts by activating caspase-7.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223257 | Nlrc4 (untagged) - Mouse NLR family, CARD domain containing 4 (Nlrc4), (10ug) |
CNY 13,210.00 |
|
| MG227430 | Nlrc4 (tGFP-tagged) - Mouse NLR family CARD domain containing 4 (Nlrc4), (10ug) |
CNY 9,220.00 |
|
| MR227430L3 | Lenti ORF clone of Nlrc4 (Myc-DDK-tagged) - Mouse NLR family, CARD domain containing 4 (Nlrc4) |
CNY 10,260.00 |
|
| MR227430L4 | Lenti ORF clone of Nlrc4 (mGFP-tagged) - Mouse NLR family, CARD domain containing 4 (Nlrc4) |
CNY 10,260.00 |
