Klrk1 (NM_001286018) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	D6H12S2489E; NKG2-D; Nkg2d
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR228611 representing NM_001286018 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCAAATGCCATAATTACGACCTCAAGCCAGCAAAGTGGGATACTTCTCAAGAACAACAGAAACAAA GATTAGCACTAACTACCAGTCAACCTGGAGAAAATGGTATCATAAGAGGAAGATACCCTATAGAAAAACT CAAAATATCTCCAATGTTCGTTGTTCGAGTCCTTGCTATAGCCTTGGCAATTCGATTCACCCTTAACACA TTGATGTGGCTTGCCATTTTCAAAGAGACGTTTCAGCCAGTTATAATTATCTTGACAGTATTGTGCAACA AGGAAGTCCCAGTTTCCTCAAGAGAGGGCTACTGTGGCCCATGCCCTAACAACTGGATATGTCACAGAAA CAACTGTTACCAATTTTTTAATGAAGAGAAAACCTGGAACCAGAGCCAAGCTTCCTGTTTGTCTCAAAAT TCCAGCCTTCTGAAGATATACAGTAAAGAAGAACAGGATTTCTTAAAGCTGGTTAAGTCCTATCACTGGA TGGGACTGGTCCAGATCCCAGCAAATGGCTCCTGGCAGTGGGAAGATGGCTCCTCTCTCTCATACAATCA GTTAACTCTGGTGGAAATACCAAAAGGATCCTGTGCTGTCTATGGCTCAAGCTTTAAGGCTTACACAGAA GACTGTGCAAATCTAAACACGTACATCTGCATGAAAAGGGCGGTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR228611 representing NM_001286018 Red=Cloning site Green=Tags(s) MSKCHNYDLKPAKWDTSQEQQKQRLALTTSQPGENGIIRGRYPIEKLKISPMFVVRVLAIALAIRFTLNT LMWLAIFKETFQPVIIILTVLCNKEVPVSSREGYCGPCPNNWICHRNNCYQFFNEEKTWNQSQASCLSQN SSLLKIYSKEEQDFLKLVKSYHWMGLVQIPANGSWQWEDGSSLSYNQLTLVEIPKGSCAVYGSSFKAYTE DCANLNTYICMKRAV TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001286018
ORF Size	675 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001286018.1, NP_001272947.1
RefSeq Size	3197 bp
RefSeq ORF	678 bp
Locus ID	27007
MW	26.3 kDa
Gene Summary	Function as an activating and costimulatory receptor involved in immunosurveillance upon binding to various cellular stress-inducible ligands displayed at the surface of autologous tumor cells and virus-infected cells. Provides both stimulatory and costimulatory innate immune responses on activated killer (NK) cells, leading to cytotoxic activity. Acts as a costimulatory receptor for T-cell receptor (TCR) in CD8(+) T-cell-mediated adaptive immune responses by amplifying T-cell activation. Stimulates perforin-mediated elimination of ligand-expressing tumor cells. Signaling involves calcium influx, culminating in the expression of TNF-alpha. Participates in NK cell-mediated bone marrow graft rejection. May play a regulatory role in differentiation and survival of NK cells. Binds to ligands belonging to various subfamilies of MHC class I-related glycoproteins including RAET1A, RAET1B, RAET1C, RAET1D, RAET1E, H60 and MULT1.[UniProtKB/Swiss-Prot Function]

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