Pitx2 (NM_001287048) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	9430085M16Rik; Brx1; Brx1b; Munc30; Otlx2; Ptx2; Rieg
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR229026 representing NM_001287048 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTTCGGTCCTAGCTCCTGGCCAGCCCCGCTCCTTGGACTCCTCCAAACATAGACTGGAGGTGCATA CAATCTCCGATACTTCCAGCCCTGAAGTCGCAGAGAAAGATAAGGGCCAGCAAGGAAAGAATGAGGATGT GGGCGCCGAGGACCCGTCCAAGAAGAAGCGGCAACGCCGGCAGAGGACTCATTTCACTAGCCAGCAGCTG CAGGAGCTGGAAGCCACTTTCCAGAGAAACCGCTACCCAGACATGTCCACTCGCGAAGAAATCGCCGTGT GGACCAACCTTACGGAAGCCCGAGTCCGGGTTTGGTTCAAGAATCGCCGGGCCAAATGGAGAAAGCGGGA ACGCAACCAGCAGGCCGAGCTGTGCAAGAATGGCTTTGGGCCGCAGTTCAACGGGCTCATGCAGCCCTAC GATGACATGTACCCCGGCTATTCGTACAACAATTGGGCTGCCAAGGGCCTCACGTCAGCGTCTCTGTCCA CCAAGAGCTTCCCCTTCTTCAACTCCATGAACGTCAATCCCCTGTCCTCTCAGAGTATGTTTTCCCCGCC CAACTCCATCTCATCTATGAGTATGTCGTCCAGCATGGTGCCCTCCGCGGTGACCGGCGTCCCGGGCTCC AGCCTCAATAGCCTGAATAACTTGAACAACCTGAGCAGCCCGTCGCTGAATTCCGCGGTGCCCACGCCCG CCTGTCCTTACGCGCCGCCGACTCCTCCGTACGTTTATAGGGACACATGTAACTCGAGCCTGGCCAGCCT GAGACTGAAAGCAAAGCAGCACTCCAGCTTCGGCTACGCCAGCGTGCAGAACCCGGCCTCCAACCTGAGT GCTTGCCAGTATGCAGTCGACCGGCCGGTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR229026 representing NM_001287048 Red=Cloning site Green=Tags(s) MASVLAPGQPRSLDSSKHRLEVHTISDTSSPEVAEKDKGQQGKNEDVGAEDPSKKKRQRRQRTHFTSQQL QELEATFQRNRYPDMSTREEIAVWTNLTEARVRVWFKNRRAKWRKRERNQQAELCKNGFGPQFNGLMQPY DDMYPGYSYNNWAAKGLTSASLSTKSFPFFNSMNVNPLSSQSMFSPPNSISSMSMSSSMVPSAVTGVPGS SLNSLNNLNNLSSPSLNSAVPTPACPYAPPTPPYVYRDTCNSSLASLRLKAKQHSSFGYASVQNPASNLS ACQYAVDRPV TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001287048
ORF Size	870 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001287048.1, NP_001273977.1
RefSeq Size	2317 bp
RefSeq ORF	873 bp
Locus ID	18741
MW	32.7 kDa
Gene Summary	Controls cell proliferation in a tissue-specific manner and is involved in morphogenesis. During embryonic development, exerts a role in the expansion of muscle progenitors. May play a role in the proper localization of asymmetric organs such as the heart and stomach. Isoform Ptx2c is involved in left-right asymmetry the developing embryo.[UniProtKB/Swiss-Prot Function]

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