Spp1 (NM_001204201) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	2AR; Apl-1; BNSP; Bsp; BSPI; Eta; ETA-1; OP; Opn; Opnl; Ric; Spp-1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR229168 representing NM_001204201 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGCTGCAGTTCTCCTGGCTGAATTCTGAGGGACTAACTACGACCATGAGATTGGCAGTGATTTGCT TTTGCCTGTTTGGCATTGCCTCCTCCCTCCCGGTGAAAGTGACTGATTCTGGCAGCTCAGAGGAGAAGAA GCTTTACAGCCTGCACCCAGATCCTATAGCCACATGGCTGGTGCCTGACCCATCTCAGAAGCAGAATCTC CTTGCGCCACAGAATGCTGTGTCCTCTGAAGAAAAGGATGACTTTAAGCAAGAAACTCTTCCAAGCAATT CCAATGAAAGCCATGACCACATGGACGACGATGATGACGATGATGATGACGATGGAGACCATGCAGAGAG CGAGGATTCTGTGGACTCGGATGAATCTGACGAATCTCACCATTCGGATGAGTCTGATGAGACCGTCACT GCTAGTACACAAGCAGACACTTTCACTCCAATCGTCCCTACAGTCGATGTCCCCAACGGCCGAGGTGATA GCTTGGCTTATGGACTGAGGTCAAAGTCTAGGAGTTTCCAGGTTTCTGATGAACAGTATCCTGATGCCAC AGATGAGGACCTCACCTCTCACATGAAGAGCGGTGAGTCTAAGGAGTCCCTCGATGTCATCCCTGTTGCC CAGCTTCTGAGCATGCCCTCTGATCAGGACAACAACGGAAAGGGCAGCCATGAGTCAAGTCAGCTGGATG AACCAAGTCTGGAAACACACAGACTTGAGCATTCCAAAGAGAGCCAGGAGAGTGCCGATCAGTCGGATGT GATCGATAGTCAAGCAAGTTCCAAAGCCAGCCTGGAACATCAGAGCCACAAGTTTCACAGCCACAAGGAC AAGCTAGTCCTAGACCCTAAGAGTAAGGAAGATGATAGGTATCTGAAATTCCGAATTTCTCATGAATTAG AGAGTTCATCTTCTGAGGTCAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR229168 representing NM_001204201 Red=Cloning site Green=Tags(s) MRLQFSWLNSEGLTTTMRLAVICFCLFGIASSLPVKVTDSGSSEEKKLYSLHPDPIATWLVPDPSQKQNL LAPQNAVSSEEKDDFKQETLPSNSNESHDHMDDDDDDDDDDGDHAESEDSVDSDESDESHHSDESDETVT ASTQADTFTPIVPTVDVPNGRGDSLAYGLRSKSRSFQVSDEQYPDATDEDLTSHMKSGESKESLDVIPVA QLLSMPSDQDNNGKGSHESSQLDEPSLETHRLEHSKESQESADQSDVIDSQASSKASLEHQSHKFHSHKD KLVLDPKSKEDDRYLKFRISHELESSSSEVN TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001204201
ORF Size	933 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001204201.1, NP_001191130.1
RefSeq Size	1475 bp
RefSeq ORF	936 bp
Locus ID	20750
MW	34.9 kDa
Gene Summary	Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.[UniProtKB/Swiss-Prot Function]

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