Kcnab2 (NM_001252655) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	F5; I2rf5; Kcnb3; kv-beta-2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR229445 representing NM_001252655 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTATCCGGAATCAACCACGGGGTCCCCAGCTCGACTCTCCCTGCGGCAGACAGGCTCCCCCGGGATGA TCTACAGGAATCTGGGCAAATCTGGCCTTCGGGTCTCCTGCCTGGGGCTTGGAACATGGGTGACCTTCGG GGGCCAGATCACGGATGAGATGGCAGAGCACCTAATGACCTTGGCCTACGATAATGGCATCAACCTGTTC GATACGGCGGAGGTCTACGCTGCTGGAAAAGCTGAAGTGGTATTAGGGAACATCATTAAGAAGAAGGGAT GGAGACGGTCCAGCCTTGTCATCACCACCAAGATCTTCTGGGGTGGAAAAGCGGAGACTGAGAGAGGCCT TTCCAGGAAGCACATAATTGAAGGACTGAAAGCGTCCCTGGAGCGGCTGCAGCTGGAGTACGTGGATGTG GTTTTTGCCAACCGCCCAGACCCCAACACGCCCATGGAAGAGACCGTGCGGGCCATGACCCATGTCATCA ACCAGGGGATGGCCATGTACTGGGGCACATCACGCTGGAGCTCCATGGAGATCATGGAGGCCTACTCGGT GGCTCGGCAGTTCAACCTGATCCCGCCCATCTGCGAGCAAGCGGAATATCACATGTTCCAGAGGGAGAAG GTGGAGGTCCAGCTGCCAGAGCTGTTCCACAAGATAGGAGTAGGTGCCATGACCTGGTCCCCTCTGGCGT GCGGCATCGTCTCAGGGAAGTATGACAGCGGGATCCCACCCTACTCCAGAGCCTCCCTGAAGGGCTACCA GTGGTTGAAGGACAAGATCCTGAGTGAGGAGGGTCGCCGCCAGCAGGCCAAGCTGAAGGAACTGCAGGCC ATTGCCGAACGCCTGGGCTGCACCCTACCCCAGCTGGCCATAGCCTGGTGCCTGAGGAATGAGGGTGTCA GCTCCGTGCTTCTGGGTGCTTCCAATGCAGAACAACTTATGGAGAACATTGGAGCAATACAGGTCCTTCC AAAATTGTCGTCTTCCATCGTCCACGAGATCGACAGCATTCTGGGCAATAAACCCTACAGCAAAAAGGAC TATAGATCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR229445 representing NM_001252655 Red=Cloning site Green=Tags(s) MYPESTTGSPARLSLRQTGSPGMIYRNLGKSGLRVSCLGLGTWVTFGGQITDEMAEHLMTLAYDNGINLF DTAEVYAAGKAEVVLGNIIKKKGWRRSSLVITTKIFWGGKAETERGLSRKHIIEGLKASLERLQLEYVDV VFANRPDPNTPMEETVRAMTHVINQGMAMYWGTSRWSSMEIMEAYSVARQFNLIPPICEQAEYHMFQREK VEVQLPELFHKIGVGAMTWSPLACGIVSGKYDSGIPPYSRASLKGYQWLKDKILSEEGRRQQAKLKELQA IAERLGCTLPQLAIAWCLRNEGVSSVLLGASNAEQLMENIGAIQVLPKLSSSIVHEIDSILGNKPYSKKD YRS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001252655
ORF Size	1059 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001252655.2
RefSeq Size	3591 bp
RefSeq ORF	1062 bp
Locus ID	16498
MW	39.8 kDa
Gene Summary	Cytoplasmic potassium channel subunit that modulates the characteristics of the channel-forming alpha-subunits (PubMed:8576199). Contributes to the regulation of nerve signaling, and prevents neuronal hyperexcitability (PubMed:11825900, PubMed:21209188). Promotes expression of the pore-forming alpha subunits at the cell membrane, and thereby increases channel activity (By similarity). Promotes potassium channel closure via a mechanism that does not involve physical obstruction of the channel pore (PubMed:8576199). Modulates the functional properties of KCNA4 (By similarity). Modulates the functional properties of KCNA5 (PubMed:8576199). Enhances KCNB2 channel activity (PubMed:8824288). Modulates the functional properties of KCNA5 (PubMed:8576199). Binds NADPH and has NADPH-dependent aldoketoreductase activity (By similarity). Has broad substrate specificity and can catalyze the reduction of methylglyoxal, 9,10-phenanthrenequinone, prostaglandin J2, 4-nitrobenzaldehyde, 4-nitroacetophenone and 4-oxo-trans-2-nonenal (in vitro) (By similarity).[UniProtKB/Swiss-Prot Function]

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