Gfra1 (NM_001285457) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	AU042498; GDNFR; GFRalpha-1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR230039 representing NM_001285457 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTCCTAGCCACTCTGTACTTCGTGCTGCCACTCCTGGATTTGCTGATGTCGGCCGAGGTGAGTGGTG GGGACCGCCTGGACTGTGTGAAAGCCAGTGATCAGTGCCTGAAGGAACAGAGCTGCAGCACCAAGTACCG CACACTGAGGCAGTGCGTGGCGGGCAAGGAAACCAACTTCAGCCTGACATCCGGCCTCGAGGCCAAGGAT GAGTGCCGCAGCGCTATGGAGGCCTTGAAGCAGAAGTCTCTCTACAACTGCCGCTGCAAGCGGGGCATGA AGAAAGAGAAGAATTGTCTGCGTATCTACTGGAGCATGTACCAGAGCCTGCAGGGAAATGACCTACTGGA AGATTCCCCATATGAGCCGGTTAACAGCAGGCTGTCAGATATATTCCGGGCAGTCCCGTTCATATCAGTG GAACACATTTCCAAAGGGAACAACTGCCTCGATGCAGCCAAGGCCTGCAACCTGGATGACACCTGCAAGA AGTACAGGTCCGCCTACATCACCCCCTGTACCACCAGCATGTCCAATGAAGTCTGCAACCGCCGCAAGTG CCACAAAGCCCTCAGGCAGTTCTTCGACAAAGTTCCAGCCAAGCACAGCTACGGGATGCTCTTCTGCTCC TGCCGGGACGTCGCCTGCACCGAGAGGCGGCGACAGACTATCGTCCCTGTGTGCTCCTATGAAGAACGAG AGAGGCCCAACTGCCTGAATCTGCAAGACTCCTGCAAGACAAATTACATCTGCAGATCTCGCCTTGCAGA TTTTTTTACCAACTGCCAGCCAGAGTCAAGGTCTGTCAGCAACTGTCTTAAGGAGAACTACGCAGACTGC CTCCTGGCCTACTCGGGACTGATTGGCACAGTCATGACTCCTAACTACATAGACTCCAGCAGCCTCAGTG TGGCGCCGTGGTGCGATTGCAGCAACAGTGGCAATGACCTGGAAGATTGCCTGAAGTTTCTGAATTTTTT TAAGGACAATACGTGTCTCAAAAATGCAATTCAAGCCTTTGGCAATGGCTCGGATGTGACCATGTGGCAG CCAGCCCCCCCAGTCCAGACCACCACTGCCACGACTACCACTGCCTTCCGGATCAAGAACAAGCCTCTAG GGCCAGCAGGCTCTGAGAATGAGATTCCCACACACGTTTTACCACCGTGTGCTAATTTGCAGGCACAGAA GCTGAAATCCAATGTATCGGGCAGTACACATCTCTGTCTTTCTGATAATGATTACGGAAAGGATGGTCTC GCTGGTGCCTCCAGCCACATAACCACAAAATCAATGGCTGCTCCTCCCAGCTGCGGTCTGAGCTCACTGC CGGTGATGGTGTTCACCGCTCTGGCTGCCCTGTTGTCTGTATCATTGGCAGAAACATCG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR230039 representing NM_001285457 Red=Cloning site Green=Tags(s) MFLATLYFVLPLLDLLMSAEVSGGDRLDCVKASDQCLKEQSCSTKYRTLRQCVAGKETNFSLTSGLEAKD ECRSAMEALKQKSLYNCRCKRGMKKEKNCLRIYWSMYQSLQGNDLLEDSPYEPVNSRLSDIFRAVPFISV EHISKGNNCLDAAKACNLDDTCKKYRSAYITPCTTSMSNEVCNRRKCHKALRQFFDKVPAKHSYGMLFCS CRDVACTERRRQTIVPVCSYEERERPNCLNLQDSCKTNYICRSRLADFFTNCQPESRSVSNCLKENYADC LLAYSGLIGTVMTPNYIDSSSLSVAPWCDCSNSGNDLEDCLKFLNFFKDNTCLKNAIQAFGNGSDVTMWQ PAPPVQTTTATTTTAFRIKNKPLGPAGSENEIPTHVLPPCANLQAQKLKSNVSGSTHLCLSDNDYGKDGL AGASSHITTKSMAAPPSCGLSSLPVMVFTALAALLSVSLAETS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001285457
ORF Size	1389 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001285457.2
RefSeq Size	4425 bp
RefSeq ORF	1392 bp
Locus ID	14585
UniProt ID	P97785
MW	51.6 kDa
Gene Summary	This gene encodes a transmembrane protein that functions as the receptor for glial cell line derived neurotrophic factor (GDNF). The encoded protein undergoes proteolytic processing to generate a glycosylphosphatidylinositol-anchored cell surface coreceptor that forms a complex with the Ret tyrosine kinase in GDNF signaling pathway. Mice lacking the encoded protein exhibit deficits in the kidneys, the enteric nervous system, and spinal motor and sensory neurons similar mice deficient in GDNF or Ret. [provided by RefSeq, Jul 2016]

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