Rora (NM_001289916) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	9530021D13Rik; nmf267; Nr1f1; ROR1; ROR2; ROR3; sg; staggerer; tmgc26
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR230057 representing NM_001289916 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTATTTTGTGATCGCAGCGATGAAAGCTCAAATTGAAATTATTCCATGCAAGATCTGTGGAGACAAAT CGTCAGGAATCCATTATGGTGTCATTACGTGTGAAGGCTGCAAGGGCTTTTTCAGGAGAAGTCAGCAGAG CAATGCCACCTACTCCTGTCCTCGTCAGAAGAACTGTTTGATTGATCGGACCAGCAGAAACCGCTGCCAG CATTGTCGGCTGCAGAAATGCCTGGCCGTGGGGATGTCTCGAGATGCTGTCAAGTTTGGTCGGATGTCCA AGAAGCAGAGAGACAGCTTGTACGCCGAGGTGCAGAAGCACCGGATGCAGCAGCAGCAGCGAGACCACCA GCAGCAGCCTGGGGAGGCGGAGCCGCTGACGCCCACCTACAACATCTCAGCCAATGGGCTGACGGAACTG CATGATGACCTCAGCACCTATATGGATGGGCACACCCCCGAGGGCAGCAAGGCCGACTCAGCCGTCAGCA GCTTCTACCTGGACATCCAGCCCTCCCCAGACCAGTCGGGATTGGACATCAATGGGATCAAACCCGAACC CATATGTGACTACACACCAGCATCTGGCTTCTTCCCCTACTGTTCCTTCACCAACGGAGAGACTTCCCCA ACCGTGTCCATGGCAGAACTAGAACACCTTGCCCAGAACATATCCAAATCCCACCTGGAAACCTGCCAGT ACTTGCGGGAAGAGCTCCAGCAGATAACGTGGCAGACCTTCCTGCAGGAGGAGATTGAAAACTACCAGAA CAAGCAGAGAGAGGTGATGTGGCAGCTGTGTGCCATCAAGATTACAGAAGCTATCCAGTATGTGGTGGAG TTTGCCAAACGCATTGATGGATTTATGGAGCTGTGTCAAAATGATCAAATTGTGCTTCTAAAAGCAGGCT CGCTAGAGGTGGTGTTTATTAGGATGTGCCGTGCCTTTGACTCTCAGAACAACACCGTGTACTTTGACGG GAAGTATGCGAGCCCCGATGTCTTCAAGTCCCTAGGTTGTGAAGACTTCATCAGCTTTGTGTTTGAATTT GGGAAGAGTTTGTGTTCTATGCACCTGACCGAAGACGAAATCGCGTTATTTTCTGCATTCGTACTGATGT CAGCGGATCGCTCGTGGCTTCAGGAAAAGGTAAAAATAGAAAAGCTGCAACAGAAAATTCAGCTGGCCCT TCAGCACGTCCTACAGAAGAACCACCGAGAAGATGGAATTCTAACCAAGCTAATATGCAAGGTGTCTACG TTAAGAGCCCTATGTGGACGACATACGGAAAAGCTAATGGCATTTAAAGCAATATACCCAGACATTGTGC GACTCCATTTTCCTCCATTATACAAGGAATTGTTCACTTCAGAATTTGAGCCAGCCATGCAAATCGATGG G ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR230057 representing NM_001289916 Red=Cloning site Green=Tags(s) MYFVIAAMKAQIEIIPCKICGDKSSGIHYGVITCEGCKGFFRRSQQSNATYSCPRQKNCLIDRTSRNRCQ HCRLQKCLAVGMSRDAVKFGRMSKKQRDSLYAEVQKHRMQQQQRDHQQQPGEAEPLTPTYNISANGLTEL HDDLSTYMDGHTPEGSKADSAVSSFYLDIQPSPDQSGLDINGIKPEPICDYTPASGFFPYCSFTNGETSP TVSMAELEHLAQNISKSHLETCQYLREELQQITWQTFLQEEIENYQNKQREVMWQLCAIKITEAIQYVVE FAKRIDGFMELCQNDQIVLLKAGSLEVVFIRMCRAFDSQNNTVYFDGKYASPDVFKSLGCEDFISFVFEF GKSLCSMHLTEDEIALFSAFVLMSADRSWLQEKVKIEKLQQKIQLALQHVLQKNHREDGILTKLICKVST LRALCGRHTEKLMAFKAIYPDIVRLHFPPLYKELFTSEFEPAMQIDG TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001289916
ORF Size	1401 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001289916.1, NP_001276845.1
RefSeq Size	10869 bp
RefSeq ORF	1404 bp
Locus ID	19883
UniProt ID	P51448
MW	53.9 kDa
Gene Summary	The protein encoded by this gene is a member of the NR1 subfamily of nuclear hormone receptors. It can bind as a monomer or as a homodimer to hormone response elements upstream of several genes to enhance the expression of those genes. The encoded protein has been shown to interact with NM23-2, a nucleoside diphosphate kinase involved in organogenesis and differentiation, as well as with NM23-1, the product of a tumor metastasis suppressor candidate gene. Also, it has been shown to aid in the transcriptional regulation of some genes involved in circadian rhythm. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2014]

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