Slc17a5 (NM_001276452) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	4631416G20Rik; 4732491M05; AST; ISSD; NSD; SD; SIALIN; SIASD; SLD
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR230068 representing NM_001276452 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGCCCCTGCTTCGGGGTCCGGCGGGAAACGACGATGAGGAGAGCTCGGACAGCACCCCGCTCCTGC CGGGCGCCCGGCAGACCGAAGCGGCTCCAGTGTGCTGCTCTGCTCGGTACAACTTAGCGATTTTGGCGTT CTGTGGTTTCTTCGTTCTCTATGCCTTACGGGTGAACCTGAGTGTTGCGTTAGTGGACATGGTAGATTCA AATACAACTCTGACTGATAATAGAACGTCTAAGGAGTGTGCGGAACATTCTGCCCCCATAAAAGTTCACC ACAATCACACAATTCCCGGTGGGTACATTGCCAGCAGGGTCGGAGGGAAGCTGCTGCTGGGCCTGGGCAT CTTAGGCACCTCCGTCTTCACCCTGTTCACACCGCTGGCCGCAGACTTAGGCGTGGTGACTCTCGTTGTG CTTAGAGCGCTGGAAGGACTGGGAGAGGGTGTTACGTTTCCAGCTATGCACGCCATGTGGTCTTCCTGGG CTCCCCCTCTGGAAAGAAGCAAGCTTCTTACCATTTCCTATGCGGGAGCACAGCTTGGGACAGTGATCTC ACTTCCTCTTTCCGGAATAATATGCTACTATATGAACTGGACTTACGTCTTCTATCTTTTTGGTATAGTT GGAATTGTCTGGTTTATTTTATGGATGTGGATAGTCAGTGATACACCAGAAACTCACAAGACAATCTCCC ATTATGAAAAAGAATACATTGTTTCATCATTAAAAAATCAGCTTTCTTCGCAGAAGGTGGTGCCGTGGGG GTCCATCTTGAAGTCACTGCCACTTTGGGCAATTGTGGTAGCACATTTCTCCTACAACTGGTCTTTTTAC ACCTTATTGACGCTACTGCCAACTTATATGAAGGAAATCCTAAGGTTCAATGTTCAAGAGAACGGGTTTT TATCTGCATTGCCTTATTTTGGCTGTTGGTTATGCATGATCCTCTGTGGTCAAGCCGCTGACTATTTAAG GGTCAAGTGGAACTTTTCAACTATAAGCGTTCGGAGAATTTTTAGCCTCGTAGGAATGGTTGGCCCTGCG GTTTTCCTAGTCGCGGCTGGATTTATAGGCTGTGACTATTCCTTGGCCGTTGCGTTCCTAACCATATCCA CGACGCTGGGAGGCTTCGCCTCTTCTGGATTTAGCATCAACCATCTGGATATCGCTCCTTCGTATGCTGG CATCCTCTTGGGCATCACAAACACGTTTGCCACTATTCCCGGGATGACGGGACCCATCATTGCTAAAAGT CTGACCCCTGATAACACTATTAGGGAATGGCAGACTGTCTTCTGTATCGCTGCTGCTATTAACGTGTTTG GGGCCATTTTCTTCACGCTGTTTGCCAAAGGGGAAGTGCAGAGCTGGGCTCTCAGTGACCACCACGGACA CAGAAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR230068 representing NM_001276452 Red=Cloning site Green=Tags(s) MRPLLRGPAGNDDEESSDSTPLLPGARQTEAAPVCCSARYNLAILAFCGFFVLYALRVNLSVALVDMVDS NTTLTDNRTSKECAEHSAPIKVHHNHTIPGGYIASRVGGKLLLGLGILGTSVFTLFTPLAADLGVVTLVV LRALEGLGEGVTFPAMHAMWSSWAPPLERSKLLTISYAGAQLGTVISLPLSGIICYYMNWTYVFYLFGIV GIVWFILWMWIVSDTPETHKTISHYEKEYIVSSLKNQLSSQKVVPWGSILKSLPLWAIVVAHFSYNWSFY TLLTLLPTYMKEILRFNVQENGFLSALPYFGCWLCMILCGQAADYLRVKWNFSTISVRRIFSLVGMVGPA VFLVAAGFIGCDYSLAVAFLTISTTLGGFASSGFSINHLDIAPSYAGILLGITNTFATIPGMTGPIIAKS LTPDNTIREWQTVFCIAAAINVFGAIFFTLFAKGEVQSWALSDHHGHRN TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001276452
ORF Size	1407 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001276452.1, NP_001263381.1
RefSeq Size	3163 bp
RefSeq ORF	1410 bp
Locus ID	235504
UniProt ID	Q8BN82
MW	51.8 kDa
Gene Summary	Transports glucuronic acid and free sialic acid out of the lysosome after it is cleaved from sialoglycoconjugates undergoing degradation, this is required for normal CNS myelination. Mediates aspartate and glutamate membrane potential-dependent uptake into synaptic vesicles and synaptic-like microvesicles. Also functions as an electrogenic 2NO(3)(-)/H(+) cotransporter in the plasma membrane of salivary gland acinar cells, mediating the physiological nitrate efflux, 25% of the circulating nitrate ions is typically removed and secreted in saliva (By similarity).[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.