Sgk3 (NM_133220) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	2510015P22Rik; A330005P07Rik; Cisk; fy; fz
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR230233 representing NM_133220 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGAGGGATTGTATCATGGACTACAAGGAGAGCTGCCCAAGTGTAAGCATTCCCAGCTCTGACGAAC ACAGAGAGAAAAAGAAGAGGTTCACGGTTTATAAAGTTCTGGTCTCTGTGGGCAGAAGCGAGTGGTTTGT CTTCAGGAGATACGCAGAGTTTGACAAACTTTACAATTCTTTAAAGAAGCAGTTTCCTGCTATGGCTCTG AAGATTCCTGCCAAGAGAATATTTGGTGATAATTTTGATCCAGATTTTATTAAACAAAGAAGAGCAGGAT TGAATGAGTTCATTCAGAACTTGGTCAGATATCCAGAGCTTTACAACCATCCAGATGTCCGAGCATTCCT TCAAATGGACAGCCCAAGACATCAGTCAGATCCATCTGAAGATGAGGATGAAAGAAGTACTTCGAAGCCA CATTCTACCTCACGGAACATCAACCTGGGACCAACTGGAAATCCTCATGCTAAACCAACTGACTTCGATT TTTTAAAAGTTATTGGAAAGGGCAGCTTTGGCAAGGTTCTTCTTGCAAAACGGAAACTGGATGGAAAATT TTATGCTGTCAAAGTGTTACAGAAAAAAATAGTTCTCAACAGAAAAGAGCAAAAACATATTATGGCTGAA CGCAATGTGCTCTTGAAAAATGTGAAGCACCCATTTTTGGTTGGATTGCACTATTCTTTCCAAACAACTG AAAAGCTTTATTTTGTTCTGGATTTTGTTAATGGAGGGGAGCTCTTCTTTCACCTCCAAAGGGAAAGGTC TTTTCCTGAACCCAGAGCGAGGTTTTATGCCGCGGAGATCGCCAGTGCCTTGGGCTACCTGCACTCCATC AAAATAGTGTACAGAGACTTGAAGCCAGAAAATATTCTTTTGGATTCAATGGGACATGTTGTCTTAACGG ATTTTGGACTTTGCAAAGAAGGAATCGCTATTTCTGATACCACCACAACTTTTTGTGGTACACCAGAGTA CCTTGCACCTGAAGTAATCAGAAAACAGCCCTATGACAACACTGTGGACTGGTGGTGCCTGGGCGCTGTT CTGTATGAGATGCTGTACGGGCTGCCTCCTTTTTACTGCCGAGATGTTGCTGAAATGTATGACAATATTC TTCACAAGCCCTTAAACTTGAGACCAGGAGTGAGTCTCACCGCCTGGTCCATTCTGGAAGAACTTCTAGA AAAAAACAGACAAAATCGACTTGGTGCCAAAGAAGACTTTCTTGAAATCCAGAATCATCCTTTTTTTGAG TCACTCAGCTGGACTGACCTCGTACAAAAAAAGATTCCACCTCCATTTAACCCTAATGTGGCTGGACCAG ATGATATCAGAAACTTTGATGCCGTCTTCACTGAAGAAACGGTTCCCTATTCAGTGTGTGTGTCTTCTGA CTATTCCATCGTGAATGCCAGTGTTCTGGAGGCAGATGATGCATTTGTTGGTTTTTCTTACGCCCCTCCT TCGGAAGACTTATTTTTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR230233 representing NM_133220 Red=Cloning site Green=Tags(s) MQRDCIMDYKESCPSVSIPSSDEHREKKKRFTVYKVLVSVGRSEWFVFRRYAEFDKLYNSLKKQFPAMAL KIPAKRIFGDNFDPDFIKQRRAGLNEFIQNLVRYPELYNHPDVRAFLQMDSPRHQSDPSEDEDERSTSKP HSTSRNINLGPTGNPHAKPTDFDFLKVIGKGSFGKVLLAKRKLDGKFYAVKVLQKKIVLNRKEQKHIMAE RNVLLKNVKHPFLVGLHYSFQTTEKLYFVLDFVNGGELFFHLQRERSFPEPRARFYAAEIASALGYLHSI KIVYRDLKPENILLDSMGHVVLTDFGLCKEGIAISDTTTTFCGTPEYLAPEVIRKQPYDNTVDWWCLGAV LYEMLYGLPPFYCRDVAEMYDNILHKPLNLRPGVSLTAWSILEELLEKNRQNRLGAKEDFLEIQNHPFFE SLSWTDLVQKKIPPPFNPNVAGPDDIRNFDAVFTEETVPYSVCVSSDYSIVNASVLEADDAFVGFSYAPP SEDLFL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_133220
ORF Size	1488 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_133220.2, NP_573483.1
RefSeq Size	5517 bp
RefSeq ORF	1491 bp
Locus ID	170755
UniProt ID	Q9ERE3
MW	57.6 kDa
Gene Summary	Serine/threonine-protein kinase which is involved in the regulation of a wide variety of ion channels, membrane transporters, cell growth, proliferation, survival and migration. Up-regulates Na(+) channels: SCNN1A/ENAC and SCN5A, K(+) channels: KCNA3/KV1.3, KCNE1, KCNQ1 and KCNH2/HERG, epithelial Ca(2+) channels: TRPV5 and TRPV6, chloride channel: BSND, creatine transporter: SLC6A8, Na(+)/dicarboxylate cotransporter: SLC13A2/NADC1, Na(+)-dependent phosphate cotransporter: SLC34A2/NAPI-2B, amino acid transporters: SLC1A5/ASCT2 and SLC6A19, glutamate transporters: SLC1A3/EAAT1, SLC1A6/EAAT4 and SLC1A7/EAAT5, glutamate receptors: GRIA1/GLUR1 and GRIK2/GLUR6, Na(+)/H(+) exchanger: SLC9A3/NHE3, and the Na(+)/K(+) ATPase. Plays a role in the regulation of renal tubular phosphate transport and bone density. Phosphorylates NEDD4L and GSK3B. Positively regulates ER transcription activity through phosphorylation of FLII. Negatively regulates the function of ITCH/AIP4 via its phosphorylation and thereby prevents CXCR4 from being efficiently sorted to lysosomes.[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.