Sntg1 (NM_001290393) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	4933426D16Rik; G1SYN; SYN4
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR230344 representing NM_001290393 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATTTCAGAACTACCTGTGAGGAGACGAAGACAGGTGTTTGCTTGCTACAAGATGGTAATCAAGAAC CTTTTAAAGTCCGGTTACACCTTGCCAGAGACCTGTTGATGCTACAGGAGCAGGATGTGCTGTGTGTGTC TGGTGAGCCTTTCTATTCTGGTGAAAGAACGGTGACCATCAGAAGACAGACAGTAGGAGGATTCGGATTA AGCATAAAGGGAGGTGCAGAACATAACATCCCGGTTGTCATTTCCAAAATATCTAAGGAGCAAAGAGCAG AGCTGTCGGGGCTGCTTTTTATTGGAGATGCAATTCTACAGATAAATGGAATTAATGTGAGGAAATGCAG ACATGAGGAGGTGGTTCAGGTTCTTCGGAATGCTGGGGAGGAAGTGACCCTGACAGTGTCATTCTTAAAA CGAGCACCTGCTTTCCTCAAACTCCCAGTTAATGAAGACTGTGCATGTGCTCCAAGTGACCAAAGCAGTG GTACCTCCTCTCCTCTTTGTGACAGTGGCTTACACCTCAACTACCATCCAAATAACACAGACACGCTTTC CTGCTCTTCGTGGCCCACATCTCCAGGCCTGAGGTGGGAGAAGCGGTGGTGTGACCTCAGGCTCATTCCC CTACTTCATGCCCGCTTCTCTCAGTATGTGCCCGGGACTGACCTGAGTCGGCAGAACGCTTTCCAAGTTG TTGCTGTGGATGGAGTCTGCAGTGGAATTCTTCAGTGCCTCTCTGCTGAAGACTGCATGGATTGGCTCCA AGCAATAGCATCTAACATTTCAAACCTCACAAAGCACAATATCAAAAAAATCAACAGAAATTTCCCTGTA AACCAGCAGATAGTCTACATGGGCTGGTGTGAAGCCCGGGAGCAGGAATCCCTCCAAGACCGTGTGTATA CACCTGTGTTTCTTGCCCTGAGGGGCTCCTGTCTTTACAGGTTCCTGTCACCTCCAGTGACCACCTGGGA CTGGACTCGAGCAGAGAAAACCTTCTCCGTGTGTGAGATCATGTGCAAGGTTCTCAAGGACAGTGACCTG CTGGATCGGCGGAAACATTGCTTCACTATGCAGTCTGAGTGTGGGGAGGACCTCTACTTCTCTGTGGAGC TAGAGAGTGACCTTGCCCAATGGGAACGAGCCTTCCAAACAGCCACCTTTCTTGAAGTGGAGCGCATACA GTGCAAGACCTATGCATGTGTGTTGGAGAGTCACTTAATGGGCCTCACTATTGACTTCAGCACAGGCTTC ATCTGCTTTGACGCAGCAACAAAGGCTGTACTTTGGAGGTATAAATTTTCTCAGCTTAAAGGCTCATCAG ATGACGGCAAAAGCAAAATCAAATTTCTGTTTCAGAATCCAGATACGAAACAGATTGAAGCAAAGGAGTT GGAATTTTCAAATTTATTTGCTGTTCTTCACTGTATTCATTCATTTTTTGCTGCCAAAGTAGCTTGTTTG GACCCTCTGTTTTTAGGTAATCAGGCTGCCACTACTGCTGCTGTCAGCTCTGCTTCTACAAGCAAGGCAA AGCACCTGGCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR230344 representing NM_001290393 Red=Cloning site Green=Tags(s) MDFRTTCEETKTGVCLLQDGNQEPFKVRLHLARDLLMLQEQDVLCVSGEPFYSGERTVTIRRQTVGGFGL SIKGGAEHNIPVVISKISKEQRAELSGLLFIGDAILQINGINVRKCRHEEVVQVLRNAGEEVTLTVSFLK RAPAFLKLPVNEDCACAPSDQSSGTSSPLCDSGLHLNYHPNNTDTLSCSSWPTSPGLRWEKRWCDLRLIP LLHARFSQYVPGTDLSRQNAFQVVAVDGVCSGILQCLSAEDCMDWLQAIASNISNLTKHNIKKINRNFPV NQQIVYMGWCEAREQESLQDRVYTPVFLALRGSCLYRFLSPPVTTWDWTRAEKTFSVCEIMCKVLKDSDL LDRRKHCFTMQSECGEDLYFSVELESDLAQWERAFQTATFLEVERIQCKTYACVLESHLMGLTIDFSTGF ICFDAATKAVLWRYKFSQLKGSSDDGKSKIKFLFQNPDTKQIEAKELEFSNLFAVLHCIHSFFAAKVACL DPLFLGNQAATTAAVSSASTSKAKHLA TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001290393
ORF Size	1551 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001290393.1, NP_001277322.1
RefSeq Size	7213 bp
RefSeq ORF	1554 bp
Locus ID	71096
UniProt ID	Q925E1
MW	58 kDa
Gene Summary	Adapter protein that binds to and probably organizes the subcellular localization of a variety of proteins. May link various receptors to the actin cytoskeleton and the dystrophin glycoprotein complex. May participate in regulating the subcellular location of diacylglycerol kinase-zeta to ensure that diacylglycerol is rapidly inactivated following receptor activation (By similarity).[UniProtKB/Swiss-Prot Function]

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