Trip10 (NM_001242391) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	AI646975; Cip4
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR230459 representing NM_001242391 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATTGGGGTACCGAGTTGTGGGATCAGTTTGAGGTGCTGGAACGCCACACGCAGTGGGGGCTGGATT TGTTGGACAAATACGTGAAGTTCGTGAAAGAACGCGCGGAGGTGGAGCAGGCTTATGCTAAGCAACTCCG GAGCCTGGTGAAAAAGTATCTTCCCAAGAGACCTACCAAAGATGACCCTGAAGTCAAGTTCAGCCAGCAG CAGTCATTTGTCCAGCTTCTCCAGGAGGTCAATGACTTCGCAGGCCAGAGAGAGCTGGTGGCTGAGAGCC TCGGTATCCGAGTGTGTCTGGAGCTGGCTAAGTATTCACAGGAGATGAAGCAGGAGAGGAAGATGCACTT CCAGGAAGGTCGTCGGGCCCAGCAGCAGCTGGAGAATGGCTTCAAACAGCTGGAGAATAGTAAGCGGAAG TTTGAACGAGATTGCCGGGAGGCTGAGAAAGCGGCTCACACTGCTGAGCGCCTAGATCAGGACATTAATG CCACCAAGGCGGATGTGGAGAAGGCCAAGCAGCAAGCTCACCTTCGGAACCACATGGCAGAAGAGAGCAA GAATGAATATGCGGCCCAGCTGCAGCGCTTCAACCGAGACCAGGCTCACTTCTACTTCTCACAGATGCCC CAGATATTCGATAAGCTGCAGGACATGGACGAACGCCGGGCCACCCGCCTGGGGGCCGGGTATGGGCTCT TATCAGAGGCTGAACTGCAGGTGGTTCCCATTATTGGCAAATGCTTGGAGGGCATGAAGGTGGCCGCCGA GTCTGTGGATGCTAAGAACGACTCACAGGTCCTCATCGAATTACACAAGTCCGGGTTTGCCCGCCCAGGG GACTTGGAATTTGAAGACTTTAGCCAAGTTATCAACCGAGTGCCTTCGGACAGCAGCCTGGGCACCCCGG ATGGCAGGCCTGAGCTCCGAGCAGCCTCCAGCCGTAGCCGCGCCAAGCGTTGGCCTTTCGGGAAAAAGAA CAAGACCGTGGCTACCGAAGATTTCAGTCACTTGCCCCCGGAGCAGCAGAGAAAGCGACTTCAGCAACAG CTGGAAGAGCGGAACCGAGAGTTGCAGAAGGAGGAGGACCAGAGGGAGGCCCTGAAGAAGATGAAAGATG TATATGAGAAAACACCACAAATGGGGGACCCTGCCAGCTTAGAGCCCCGCATTGCAGAGACCCTGGGCAA CATTGAGAGGCTGAAGTTGGAAGTGCAGAAGTATGAGGCTTGGTTGGCAGAAGCTGAAAGCCGGGTCCTC AGTAACCGAGGGGACAGCCTAAGCCGTCACGCTAGGCCCCCTGATCCCCCAACTACTGCCCCACCTGATA GCAGCAGTAGCAGCACCAACAGTGGATCCCAGGACAATAAGGAGAGCTCAGAAGAGCCCCCTTCAGAAGG CCAGGACACCCCCATCTATACTGAGTTCGATGAGGACTTTGAGGAGCCTGCATCCCCTATCGGCCAGTGT GTGGCTATCTACCATTTTGAAGGATCCAGCGAGGGAACCGTCTCCATGTCCGAGGGGGAAGACCTCAGCC TGATGGAGGAAGACAAGGGTGATGGATGGACGCGGGTCAGGAGGAAACAGGGAGCTGAGGGCTACGTGCC CACCTCTTACCTCCGAGTCACACTCAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR230459 representing NM_001242391 Red=Cloning site Green=Tags(s) MDWGTELWDQFEVLERHTQWGLDLLDKYVKFVKERAEVEQAYAKQLRSLVKKYLPKRPTKDDPEVKFSQQ QSFVQLLQEVNDFAGQRELVAESLGIRVCLELAKYSQEMKQERKMHFQEGRRAQQQLENGFKQLENSKRK FERDCREAEKAAHTAERLDQDINATKADVEKAKQQAHLRNHMAEESKNEYAAQLQRFNRDQAHFYFSQMP QIFDKLQDMDERRATRLGAGYGLLSEAELQVVPIIGKCLEGMKVAAESVDAKNDSQVLIELHKSGFARPG DLEFEDFSQVINRVPSDSSLGTPDGRPELRAASSRSRAKRWPFGKKNKTVATEDFSHLPPEQQRKRLQQQ LEERNRELQKEEDQREALKKMKDVYEKTPQMGDPASLEPRIAETLGNIERLKLEVQKYEAWLAEAESRVL SNRGDSLSRHARPPDPPTTAPPDSSSSSTNSGSQDNKESSEEPPSEGQDTPIYTEFDEDFEEPASPIGQC VAIYHFEGSSEGTVSMSEGEDLSLMEEDKGDGWTRVRRKQGAEGYVPTSYLRVTLN TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001242391
ORF Size	1638 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001242391.1, NP_001229320.1
RefSeq Size	2287 bp
RefSeq ORF	1641 bp
Locus ID	106628
UniProt ID	Q8CJ53
MW	63 kDa
Gene Summary	Required to coordinate membrane tubulation with reorganization of the actin cytoskeleton during endocytosis. Binds to lipids such as phosphatidylinositol 4,5-bisphosphate and phosphatidylserine and promotes membrane invagination and the formation of tubules. Also promotes CDC42-induced actin polymerization by recruiting WASL/N-WASP which in turn activates the Arp2/3 complex. Actin polymerization may promote the fission of membrane tubules to form endocytic vesicles. Required for the formation of podosomes, actin-rich adhesion structures specific to monocyte-derived cells. May be required for the lysosomal retention of FASLG/FASL (By similarity). Required for translocation of GLUT4 to the plasma membrane in response to insulin signaling.[UniProtKB/Swiss-Prot Function]

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