Nprl3 (NM_001284359) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Aag; CGTHBA; HS-26; HS-40; m(alpha)RE; Mare; Phg; Prox1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR230584 representing NM_001284359 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGGACAATACCAGCCCCATCAGCGTGATCCTGGTGAGCTCGGGCAGCAGGGGCAATAAGCTGCTCT TCAGGTACCCCTTCCAGAGGAGCCAGGAGCACCCCGCGTCCCAGACAAATAAACCACGGAGCAGATATGC TGTCAACAACACTGGCGAACATGCTGATGACCAGGACGGTGACTCCAGCAAGCCATGTCCTCCAACCGAT GAGCAATTGGTTGCAGGGTTCTCGGATGTTATTCTGGCAACAATTTTGGCAACCAAATCTGAAATGTGCG GCCAAAAATTTGAACTGAAGATTGATAATGTACGGTTTGTTGGGCACCCAACACTGCTGCAGCATGCTCT GGGGCAGGTCTCCAAAACAGATCCATCTCCAAAGCGGGAAGCTCCGACCATGATTCTTTTTAACGTGGTA TTTGCACTGAGGGCCAATGCTGATCCATCAGTGATAAACTGTCTACACAACTTGTCCCGCCGAATTGCTA CTGTCCTGCAGCATGAGGAGCGCCGCTGCCAGTACCTCACTCGAGAGGCCAAGCTGATCCTAGCACTTCA GGATGAGGTGTCTGCTATGGCTGATGCAAATGAAGGTCCTCAGTCCCCATTCCAGCACATCCTACCCAAG TGCAAGCTGGCTCGGGACCTCAAAGAAGCTTATGACAGCCTGTGCACATCTGGTGTGGTACGGCTTCACA TTAACAGCTGGCTGGAAGTGAGTTTCTGTCTGCCCCACAAGATCCACTATGCAGCTTCAAGTCTGATTCC TCCTGAGGCTATTGAACGGAGCCTGAAAGCCATCCGCCCGTACCATGCCTTGCTACTTCTCAGTGACGAG AAGTCTCTGCTGAGTGAGCTTCCCATTGACTGCTCCCCGGCTCTGGTGCGGGTGATCAAGACCACGTCTG CTGTGAAGAACCTGCAGCAGCTAGCCCAGGATGCCGATCTGGCCTTGCTGCAGGTCTTCCAGTTGGCAGC TCACCTGGTATACTGGGGCAAGGCTGTCATCATCTACCCACTGTGTGAGAACAACGTCTATGTCATGTCT CCCAATGCCAGTGTGTGTCTGTACTCCCCGCTAGCTGAACAGTTCTCCCGGCAGTTCCCGTCCCATGACC TGCCATCTGTCCTGGCCAAGTTTTCCTTGCCTGTTTCTTTATCGGAGTTCAGGAGCCCTCTGGCTCCCCC TGCACAGGAGACCCAGCTCATCCAGATGGTGGTGTGGATGCTGCAGCGCCGGCTCCTCATCCAGCTGCAT ACCTATGTTTGCCTAATGGCCTCACCCAGCGAAGAGGAGCCCCGACTGCGAGAGGATGATGTCCCCTTCA CAGCCAGAGTTGGTGGCCGCAGCCTCAGCACACCCAATGCTCTAAGCTTTGGCTCCCCAACCAGCAGTGA CGACATGACCCTTACCAGCCCCAGTATGGACAACTCAAGTGCTGAGCTGCTCCCCAGTGGGGACTCACCA CTGAACAAGAGGATGACAGAGAACCTGCTGGCTAGCCTCTCAGAGCATGAGCGGGCTGCTATCCTCAATG TGCCTGCAGCCCAAAACCCTGAGGACCTCCGCATGTTTGCCAGGCTCCTTCACTACTTCCGTGGCCGCCA CCATCTGGAGGAGATCATGTACAATGAGAACACTCGGCGCTCCCAGCTACTCATGCTCTTTGACAAATTC CGCAGTGTGCTGGTGGTGACCACCCATGAGGACCCTGTTATTGCCGTCTTCCAGGCACTGCTCACA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR230584 representing NM_001284359 Red=Cloning site Green=Tags(s) MGDNTSPISVILVSSGSRGNKLLFRYPFQRSQEHPASQTNKPRSRYAVNNTGEHADDQDGDSSKPCPPTD EQLVAGFSDVILATILATKSEMCGQKFELKIDNVRFVGHPTLLQHALGQVSKTDPSPKREAPTMILFNVV FALRANADPSVINCLHNLSRRIATVLQHEERRCQYLTREAKLILALQDEVSAMADANEGPQSPFQHILPK CKLARDLKEAYDSLCTSGVVRLHINSWLEVSFCLPHKIHYAASSLIPPEAIERSLKAIRPYHALLLLSDE KSLLSELPIDCSPALVRVIKTTSAVKNLQQLAQDADLALLQVFQLAAHLVYWGKAVIIYPLCENNVYVMS PNASVCLYSPLAEQFSRQFPSHDLPSVLAKFSLPVSLSEFRSPLAPPAQETQLIQMVVWMLQRRLLIQLH TYVCLMASPSEEEPRLREDDVPFTARVGGRSLSTPNALSFGSPTSSDDMTLTSPSMDNSSAELLPSGDSP LNKRMTENLLASLSEHERAAILNVPAAQNPEDLRMFARLLHYFRGRHHLEEIMYNENTRRSQLLMLFDKF RSVLVVTTHEDPVIAVFQALLT TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001284359
ORF Size	1746 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001284359.1, NP_001271288.1
RefSeq Size	2904 bp
RefSeq ORF	1749 bp
Locus ID	17168
UniProt ID	Q8VIJ8
MW	65.3 kDa
Gene Summary	As a component of the GATOR1 complex functions as an inhibitor of the amino acid-sensing branch of the TORC1 pathway. The GATOR1 complex strongly increases GTP hydrolysis by RRAGA and RRAGB within RRAGC-containing heterodimers, thereby deactivating RRAGs, releasing mTORC1 from lysosomal surface and inhibiting mTORC1 signaling. The GATOR1 complex is negatively regulated by GATOR2 the other GATOR subcomplex in this amino acid-sensing branch of the TORC1 pathway.[UniProtKB/Swiss-Prot Function]

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