Recql (NM_001204906) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	RecQ1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR230749 representing NM_001204906 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCATCCGTCTCAGCTTTGACAGAGGAACTGGAATCCGTAGCCAGTGAGCTTCATGCCATTGACATCC AGATTCAGGAACTCACAGAGAGGCGGCAAGAGCTCCTTCAGAGAAAGTCAGTCCTGACAGGGAAAATCAA ACAGTACTTGGAGGACTCTTCGGCTGAGGCGAGCAGCGACTTGGACACATCACCAGCTGCTTGGAATAAA GAAGATTTTCCATGGTTCGGAAAGGTAAAAGATGTGCTGCAAAATGTTTTTAAACTGCAAAAATTCAGAC CTCTGCAGCTGGAAACCATCAATGTCACCATGGCTAGAAAGGATATCTTTCTTGTCATGCCCACAGGAGG AGGGAAGAGCTTATGCTACCAGCTACCTGCGCTGTGCTCAGACGGTTTTACACTTGTGATTTGCCCACTC ATCTCTCTTATGGAAGATCAGCTGATGGTTTTAAAACAGTTGGGAATTTCAGCTACTATGTTGAATGCTT CCAGCTCTAAGGAACATGTAAAATGGGTGCACGCTGAAATGGTGAATAAAAACTCCCAGTTAAAGCTGAT TTATGTAACTCCAGAGAAAATTGCAAAAAGCAAGATGTTCATGTCAAGACTGGAGAAAGCCTATGAAGCC GGGAGGCTGACGGGAGCGGCAGTGGATGAAGTGCATTGCTGCAGTCAGTGGGGTCATGACTTCAGGCCTG ATTATAAGGCTCTTGGCATCTTGAAGCGCCAGTTTCCCAACGCCTCACTGATGGGGCTGACTGCAACAGC AACCAACCATGTTCTGAAGGATGTACAGAAAATCCTGTGTGTGGGAAAGTGTTTGACTTTTACAGCGTCT TTCAACCGGCCAAATCTTTTTTATGAGGTTCGGCAAAAGCCCTCAAGTGCTGAAGACTTTACTGAGGACA TTGTCAAGCTAATTAATGGACGGTACAAAGGACAATCAGGAATCATATACTGTTTCTCTCAGAAAGACTC TGAACAAATTACCATCAGTTTACAGAAGTTGGGAATTCATGCAGGCACTTACCATGCCAATATGGAACCA GAAGATAAGACCAAGGTTCATACACAGTGGTCAGCCAATGAGCTTCAGGTGGTAGTGGCAACAGTCGCAT TTGGCATGGGAATTGATAAGCCAGACGTGAGGTTTGTCATCCATCATTCAATGAGCAAATCCATGGAGAA TTACTATCAAGAGAGCGGGCGTGCCGGTCGCGATGACTCGAGAGCAGACTGTATTCTGTATTACGGCTTT GGGGACATATTCAGGATCAGCTCGATGGTGGTCATGGAGAACGTGGGGCAGCAGAAGCTGTATGAGATGG TGTCATACTGCCAGAATGTCAGCAAGTGTCGCCGTGTGTTAATAGCACAGCATTTTGATGAAGTGTGGAA CGCAGATGCCTGTAACAAAATGTGTGACAACTGCTGTAAAGACGTTTCGTTTGAGAAAAAGAATGTGACA CAGCACTGCCGGGACCTGATAAAGATTCTGAAGCAGGCTGAAGGCCTGAATGAAAAGCTGACCCCGCTGA AGCTGATTGATGCTTGGATGGGAAAGGGAGCAGCCAAGTTGAGAGTGGCTGGCGTTGTTGCCCCGGCCCT TCCCCGGGAAGACCTGGAGAGAATCGTCGCCCACGCCCTCCTGCAGCAATATCTCAAAGAAGACTACAGC TTCACGGCTTACGCCACCATCTCCTATCTGAAGGTGGGACCCAGAGCTTGTCTTCTCAGCAACGAAGCCC ATGCTGTCACTATGCAAGTGAAGAAGTCAGCACAGAGCAGCGTTAGGGGTGCACTGTCTGAAGCTCGTCA GGTGGAACAAGTGGATTCGAAGGGGGAAGAGCAAAGCTCAGACACACCAGACAAGAGCATCAGATCCCAT TACAGATGGTTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR230749 representing NM_001204906 Red=Cloning site Green=Tags(s) MASVSALTEELESVASELHAIDIQIQELTERRQELLQRKSVLTGKIKQYLEDSSAEASSDLDTSPAAWNK EDFPWFGKVKDVLQNVFKLQKFRPLQLETINVTMARKDIFLVMPTGGGKSLCYQLPALCSDGFTLVICPL ISLMEDQLMVLKQLGISATMLNASSSKEHVKWVHAEMVNKNSQLKLIYVTPEKIAKSKMFMSRLEKAYEA GRLTGAAVDEVHCCSQWGHDFRPDYKALGILKRQFPNASLMGLTATATNHVLKDVQKILCVGKCLTFTAS FNRPNLFYEVRQKPSSAEDFTEDIVKLINGRYKGQSGIIYCFSQKDSEQITISLQKLGIHAGTYHANMEP EDKTKVHTQWSANELQVVVATVAFGMGIDKPDVRFVIHHSMSKSMENYYQESGRAGRDDSRADCILYYGF GDIFRISSMVVMENVGQQKLYEMVSYCQNVSKCRRVLIAQHFDEVWNADACNKMCDNCCKDVSFEKKNVT QHCRDLIKILKQAEGLNEKLTPLKLIDAWMGKGAAKLRVAGVVAPALPREDLERIVAHALLQQYLKEDYS FTAYATISYLKVGPRACLLSNEAHAVTMQVKKSAQSSVRGALSEARQVEQVDSKGEEQSSDTPDKSIRSH YRWL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001204906
ORF Size	1902 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001204906.1, NP_001191835.1
RefSeq Size	3067 bp
RefSeq ORF	1905 bp
Locus ID	19691
MW	71.8 kDa
Gene Summary	Isoform alpha is a DNA helicase that may play a role in the repair of DNA that is damaged by ultraviolet light or other mutagens. Isoform beta may have important roles in the meiotic process. Both isoforms exhibit a magnesium-dependent ATP-dependent DNA-helicase activity that unwinds single- and double-stranded DNA in a 3'-5' direction.[UniProtKB/Swiss-Prot Function]

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