Bcl11b (NM_001286343) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	9130430L19Rik; AI604821; B630002E05Rik; BCL-11B; Ctip2; Rit1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR230897 representing NM_001286343 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCCGCCGCAAACAGGGCAACCCGCAGCACTTGTCCCAGAGGGAACTCATCACGCGTAAAGATGAGC CTTCCAGCTACATTTGCACAACATGCAAGCAGCCCTTCAACAGCGCCTGGTTCCTGCTGCAGCACGCACA GAACACACATGGCTTCCGAATCTACCTGGAGCCTGGGCCGGCCAGCACCTCGCTCACGCCCAGGCTCACC ATCCCGCCACCGCTCGGGCCGGAGACCGTGGCGCAGTCCCCACTCATGAATTTCCTGGGGGACAGCAATC CTTTCAACCTGCTGCGCATGACGGGCCCCATCCTGCGGGACCACCCTGGCTTCGGTGAGGGCCGCTTGCC AGGTACGCCACCGCTCTTCAGCCCACCGCCACGCCATCACTTGGACCCACACCGCCTCAGTGCAGAGGAG ATGGGGCTCGTGGCCCAGCACCCCAGTGCCTTCGACCGAGTCATGCGCCTGAACCCCATGGCCATAGACT CTCCTGCCATGGACTTCTCCCGGCGGCTGCGAGAACTGGCCGGCAACAGCTCCACGCCGCCGCCCGTGTC CCCAGGCCGTGGCAACCCTATGCACCGGCTGCTGAACCCTTTCCAGCCCAGTCCCAAGTCCCCGTTCCTC AGCACGCCACCGCTGCCACCCATGCCTGCGGGCACACCGCCACCGCAGCCGCCTGCCAAGAGCAAGTCCT GTGAGTTCTGCGGCAAGACCTTCAAGTTCCAGAGCAATCTCATCGTGCACCGGCGCAGCCACACGGGCGA GAAGCCCTACAAGTGCCAGCTGTGCGACCATGCGTGCTCGCAGGCGAGCAAGCTCAAGCGCCACATGAAG ACGCACATGCACAAGGCGGGCTCTCTGGCTGGCCGCTCAGACGACGGGCTCTCAGCTGCCAGCTCCCCTG AGCCGGGCACCAGCGAGCTGCCAGGTGACCTGAAAGCGGCCGATGGCGACTTCCGCCACCATGAGAGCGA CCCATCTCTGGGCCCCGAGCCTGAGGACGACGAGGACGAGGAGGAGGAAGAAGAGGAGCTGCTGCTGGAG AACGAGAGCCGGCCTGAGTCGAGCTTCAGCATGGACTCGGAGCTGGGCCGTGGCCGCGAGAACGGAGGTG GCGTGCCACCGGGGGTGGCGGGCGCAGGGGCTGCAGCTGCGGCTCTGGCGGATGAGAAGGCTCTGGCCCT GGGCAAGGTGATGGAGGACGCAGGGCTGGGCGCACTGCCGCAGTATGGGGAGAAGCGGGGCGCCTTCCTG AAGCGTGCAGGCGACACGGGTGATGCCGGAGCTGTTGGCTGTGGGGACGCGGGTGCACCGGGTGCAGTGA ACGGGCGCGGCGGGGCCTTCGCGCCAGGCGCAGAGCCCTTTCCAGCTCTCTTCCCACGCAAGCCAGCACC GCTGCCCAGCCCTGGGCTCGGTGGTCCCGCGCTGCACGCGGCCAAGCGCATCAAGGTGGAGAAAGACCTG GAGCTGCCACCTGCCGCCCTCATCCCATCTGAGAACGTGTACTCGCAGTGGCTCGTGGGCTACGCAGCAT CGCGCCACTTCATGAAGGACCCATTCCTGGGCTTCACGGATGCGCGCCAGTCGCCTTTCGCCACATCGTC GGAACATTCCTCTGAGAACGGCAGCCTGCGCTTCTCAACGCCACCCGGGGACCTGCTGGACGGCGGGCTG TCCGGGCGCAGTGGCACGGCGAGCGGGGGCAGCACACCTCACCTGGGTGGTCCGGGTCCTGGGAGGCCGA GCTCCAAGGAGGGCCGCCGCAGCGACACATGTGAGTACTGCGGCAAGGTCTTCAAGAACTGTAGCAACCT GACGGTGCACCGGAGGAGCCACACCGGCGAGCGGCCTTACAAGTGCGAGCTGTGCAACTACGCGTGCGCG CAGAGCAGCAAGCTCACGCGCCACATGAAGACGCACGGGCAGATCGGCAAGGAGGTGTACCGCTGCGACA TCTGCCAGATGCCCTTCAGCGTCTACAGCACCCTGGAGAAACACATGAAAAAGTGGCACGGTGAACACTT GCTGACTAATGATGTCAAAATCGAGCAGGCTGAGAGGAGC AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR230897 representing NM_001286343 Red=Cloning site Green=Tags(s) MSRRKQGNPQHLSQRELITRKDEPSSYICTTCKQPFNSAWFLLQHAQNTHGFRIYLEPGPASTSLTPRLT IPPPLGPETVAQSPLMNFLGDSNPFNLLRMTGPILRDHPGFGEGRLPGTPPLFSPPPRHHLDPHRLSAEE MGLVAQHPSAFDRVMRLNPMAIDSPAMDFSRRLRELAGNSSTPPPVSPGRGNPMHRLLNPFQPSPKSPFL STPPLPPMPAGTPPPQPPAKSKSCEFCGKTFKFQSNLIVHRRSHTGEKPYKCQLCDHACSQASKLKRHMK THMHKAGSLAGRSDDGLSAASSPEPGTSELPGDLKAADGDFRHHESDPSLGPEPEDDEDEEEEEEELLLE NESRPESSFSMDSELGRGRENGGGVPPGVAGAGAAAAALADEKALALGKVMEDAGLGALPQYGEKRGAFL KRAGDTGDAGAVGCGDAGAPGAVNGRGGAFAPGAEPFPALFPRKPAPLPSPGLGGPALHAAKRIKVEKDL ELPPAALIPSENVYSQWLVGYAASRHFMKDPFLGFTDARQSPFATSSEHSSENGSLRFSTPPGDLLDGGL SGRSGTASGGSTPHLGGPGPGRPSSKEGRRSDTCEYCGKVFKNCSNLTVHRRSHTGERPYKCELCNYACA QSSKLTRHMKTHGQIGKEVYRCDICQMPFSVYSTLEKHMKKWHGEHLLTNDVKIEQAERS SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001286343
ORF Size	2070 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001286343.1, NP_001273272.1
RefSeq Size	7341 bp
RefSeq ORF	2073 bp
Locus ID	58208
UniProt ID	Q99PV8
MW	74.7 kDa
Gene Summary	Key regulator of both differentiation and survival of T-lymphocytes during thymocyte development in mammals (PubMed:12717433). Essential in controlling the responsiveness of hematopoietic stem cells to chemotactic signals by modulating the expression of receptors CCR7 and CCR9, which direct the movement of progenitor cells from the bone marrow to the thymus (By similarity). Is a regulator of IL2 promoter and enhances IL2 expression in activated CD4(+) T-lymphocytes (PubMed:16809611). Tumor-suppressor protein involved in T-cell lymphomas. May function on the P53-signaling pathway. Repress transcription through direct, TFCOUP2-independent binding to a GC-rich response element.[UniProtKB/Swiss-Prot Function]

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