Dlg1 (NM_001252436) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	B130052P05Rik; Dlgh1; E-dlg/SAP97; mKIAA4187; SAP-97; SAP97
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231227 representing NM_001252436 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAATTACATTTTCGGAAACAACACACTTCTGTACTCTCGCGCCAGTCGAGGAGGCAATGCTAGCTCTA GCCATGGCTCAGTAGGCCCTAAGCAGAAACACTGGGCAAAAAAGGGCTCGTCAGATGAACTGCAAGCCGA GCCAGAGCCTTCCCGCTGGCAGCAGATAGTTGCATTCTTCACTCGAAGACACAGCTTTATTGACTGCATC TCGGTAGCCACCAGCTCCACCCAGCCCACAGAAGCTGTTCCTCCCTCCTCTCCCATTGTCCCTGTGACCC CTGCCCTGCCAGTCCCTGCTGAGAGTACTGTCGTCCTGCCCTCCGCACCACAGGCAAATCCTCCTCCAGT GCTGGTCAACACAGACAGCTTAGAGACACCAACTTATGTTAATGGCACTGATGCAGATTATGAATATGAG GAAATCACACTTGAAAGGGGAAATTCGGGTCTTGGTTTCAGCATTGCAGGAGGTACAGACAACCCACACA TTGGAGATGACTCAAGTATTTTCATCACCAAAATTATCACAGGCGGAGCGGCTGCCCAGGATGGAAGATT GCGGGTAAATGACTGTATACTGAGAGTAAATGAAGCAGACGTTCGTGATGTAACCCACAGCAAAGCAGTG GAGGCATTAAAAGAAGCTGGATCTATTGTGCGATTGTATGTGAAAAGGCGGAAGCCAGCATCAGAAAAAA TCATGGAAATAAAGCTCATTAAAGGTCCTAAAGGTCTTGGGTTCAGCATTGCTGGAGGTGTTGGAAATCA GCACATTCCTGGTGATAACAGCATCTATGTAACCAAAATAATTGAAGGAGGTGCAGCACACAAGGATGGC AAGCTTCAGATTGGAGATAAGCTTCTAGCAGTGAACAGTGTGTGTTTAGAAGAAGTTACTCATGAAGAAG CAGTGACTGCCTTAAAGAATACATCTGATTTTGTTTATTTGAAAGTGGCAAAACCAACAAGTATGTATAT AAATGATGGCTATGCACCACCTGACATCACTAATTCTTCTTCTCAATCTGTTGACAACCATGTCAGCCCG TCCTCCTGCTTGGGCCAGACGCCAACGTCACCAGCCAGGTACTCACCCATTTCTAAAGCTGTGCTCGGAG ATGACGAGATCACTAGGGAACCTAGAAAAGTTGTTCTTCATCGTGGCTCAACAGGACTTGGTTTTAACAT TGTGGGAGGTGAAGATGGAGAAGGGATTTTTATCTCCTTCATCCTTGCTGGCGGACCTGCTGATCTAAGT GGAGAGCTCAGAAAAGGAGATCGCATCATATCGGTGAACAGTGTTGACCTCAGAGCTGCAAGTCACGAAC AAGCAGCAGCTGCACTAAAGAACGCAGGCCAAGCCGTCACCATCGTTGCGCAATATCGACCCGAAGAGTA CAGTCGTTTTGAAGCTAAAATCCATGACTTACGGGAGCAGATGATGAATAGCAGTGTCAGTTCAGGGTCA GGGTCTCTTCGAACCAGCCAGAAGCGCTCCCTCTATGTCAGAGCCCTCTTTGATTATGACAAGACTAAGG ACAGCGGGCTTCCCAGTCAAGGACTGAACTTCCGCTTTGGAGACATCCTCCATGTCATCAATGCTTCTGA CGACGAGTGGTGGCAAGCCAGGCAGGTCACCCCAGACGGGGAGAGTGACGAAGTCGGAGTGATTCCTAGT AAACGAAGAGTTGAGAAGAAGGAACGAGCCCGATTAAAAACGGTCAAATTCAATTCTAAAACAAGAGGAG ATAAAGGGGAGATCCCTGACGACATGGGATCAAAAGGCCTGAAGCACGTAACTTCTAATGCCAGCGATAG TGAAAGTAGTTACCTAATCTTGATTACAGATGAATATGGCTGCTCAAAAGGTGGTCAAGAAGAATATGTT TTATCTTATGAGCCAGTGAATCAACAAGAAGTTAATTATACCCGACCAGTCATCATATTAGGACCTATGA AAGACAGAGTAAATGATGACTTAATCTCAGAATTTCCTGACAAATTTGGATCCTGTGTCCCTCATACAAC TAGACCGAAGCGTGACTATGAGGTGGATGGACGAGATTATCATTTTGTGACTTCAAGGGAACAGATGGAA AAGGATATTCAGGAGCATAAGTTCATTGAAGCCGGCCAGTATAACAACCATCTGTATGGGACGAGCGTGC AGTCCGTGCGAGCAGTGGCAGAGAAGGGCAAACATTGTATCCTTGATGTGTCTGGAAATGCCATAAAGAG GTTGCAGATTGCACAGCTTTATCCAATATCTATTTTTATTAAACCCAAATCCATGGAAAATATCATGGAA ATGAACAAGCGCCTAACAGAAGAGCAGGCCAGAAAAACATTTGAGAGAGCCATGAAGCTGGAGCAGGAGT TCACTGAGCATTTCACAGCTATTGTCCAGGGAGACACGCTGGAGGACATTTACAACCAAGTGAAACAGAT CATCGAAGAACAGTCTGGGCCTTACATCTGGGTCCCAGCGAAAGAAAAGCTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231227 representing NM_001252436 Red=Cloning site Green=Tags(s) MNYIFGNNTLLYSRASRGGNASSSHGSVGPKQKHWAKKGSSDELQAEPEPSRWQQIVAFFTRRHSFIDCI SVATSSTQPTEAVPPSSPIVPVTPALPVPAESTVVLPSAPQANPPPVLVNTDSLETPTYVNGTDADYEYE EITLERGNSGLGFSIAGGTDNPHIGDDSSIFITKIITGGAAAQDGRLRVNDCILRVNEADVRDVTHSKAV EALKEAGSIVRLYVKRRKPASEKIMEIKLIKGPKGLGFSIAGGVGNQHIPGDNSIYVTKIIEGGAAHKDG KLQIGDKLLAVNSVCLEEVTHEEAVTALKNTSDFVYLKVAKPTSMYINDGYAPPDITNSSSQSVDNHVSP SSCLGQTPTSPARYSPISKAVLGDDEITREPRKVVLHRGSTGLGFNIVGGEDGEGIFISFILAGGPADLS GELRKGDRIISVNSVDLRAASHEQAAAALKNAGQAVTIVAQYRPEEYSRFEAKIHDLREQMMNSSVSSGS GSLRTSQKRSLYVRALFDYDKTKDSGLPSQGLNFRFGDILHVINASDDEWWQARQVTPDGESDEVGVIPS KRRVEKKERARLKTVKFNSKTRGDKGEIPDDMGSKGLKHVTSNASDSESSYLILITDEYGCSKGGQEEYV LSYEPVNQQEVNYTRPVIILGPMKDRVNDDLISEFPDKFGSCVPHTTRPKRDYEVDGRDYHFVTSREQME KDIQEHKFIEAGQYNNHLYGTSVQSVRAVAEKGKHCILDVSGNAIKRLQIAQLYPISIFIKPKSMENIME MNKRLTEEQARKTFERAMKLEQEFTEHFTAIVQGDTLEDIYNQVKQIIEEQSGPYIWVPAKEKL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001252436
ORF Size	2502 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001252436.1, NP_001239365.1
RefSeq Size	4513 bp
RefSeq ORF	2505 bp
Locus ID	13383
MW	92.1 kDa
Gene Summary	Essential multidomain scaffolding protein required for normal development. Recruits channels, receptors and signaling molecules to discrete plasma membrane domains in polarized cells. Regulates the excitability of cardiac myocytes by modulating the functional expression of Kv4 channels (By similarity). Functional regulator of Kv1.5 channel (By similarity). May play a role in adherens junction assembly, signal transduction, cell proliferation, synaptogenesis and lymphocyte activation.[UniProtKB/Swiss-Prot Function]

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