Abl1 (NM_001283047) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Abl; AI325092; c-Abl; E430008G22Rik
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231317 representing NM_001283047 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCGCACCGACATCACCATGAAGCACAAGTTGGGTGGAGGCCAGTACGGGGAGGTGTACGAGGGCG TTTGGAAGAAGTACAGCCTCACTGTGGCCGTGAAGACCTTGAAGGAGGACACCATGGAGGTGGAGGAGTT CCTGAAGGAAGCGGCGGTGATGAAGGAGATCAAACACCCTAACCTGGTGCAGCTGCTAGGGGTGTGTACC CGGGAACCACCATTCTACATAATCACTGAGTTCATGACCTATGGGAACCTGCTGGACTACCTGAGGGAGT GTAACCGGCAGGAGGTGAGCGCCGTGGTACTGCTCTACATGGCCACACAGATCTCATCAGCCATGGAGTA CTTGGAGAAGAAGAACTTCATCCACAGAGACCTTGCTGCCCGGAACTGCCTGGTAGGGGAAAACCACTTG GTGAAGGTGGCTGATTTTGGCCTGAGCAGGTTGATGACAGGGGACACCTACACGGCCCATGCTGGAGCCA AATTCCCCATCAAATGGACCGCACCTGAGAGCCTGGCCTACAACAAGTTCTCCATCAAGTCGGACGTGTG GGCATTTGGAGTATTGCTCTGGGAGATTGCTACCTATGGCATGTCACCTTACCCGGGAATTGACCTGTCT CAGGTTTATGAGCTGCTGGAAAAAGACTACCGCATGGAGCGCCCTGAAGGCTGCCCGGAGAAGGTCTACG AGCTCATGCGAGCATGTTGGCAGTGGAACCCCTCTGACCGGCCCTCCTTTGCTGAAATCCACCAAGCCTT TGAAACCATGTTCCAGGAATCCAGTATCTCAGATGAGGTGGAGAAGGAGCTGGGGAAACGAGGCACGAGA GGAGGTGCTGGGAGTATGCTGCAGGCCCCAGAGCTGCCCACCAAGACCAGAACCTGCAGGAGAGCAGCTG AGCAGAAAGATGCGCCTGACACCCCTGAGCTGCTCCACACGAAGGGCCTGGGAGAAAGCGATGCACTGGA CAGTGAGCCTGCTGTATCGCCACTGCTTCCTCGGAAAGAGCGCGGGCCCCCAGACGGCAGCCTAAATGAA GATGAGCGCCTTCTCCCCAGAGACAGAAAGACCAACCTGTTCAGCGCTTTGATCAAGAAGAAGAAGAAAA TGGCGCCGACGCCCCCTAAGCGCAGCAGTTCCTTCCGAGAGATGGATGGCCAGCCAGACCGCAGAGGGGC TAGTGAGGATGACAGCAGGGAACTCTGCAATGGACCACCAGCTCTCACCTCAGACGCAGCAGAGCCTACC AAGTCCCCAAAGGCCAGCAATGGGGCTGGCGTCCCTAATGGAGCCTTCCGGGAGCCGGGCAACTCAGGCT TCCGTTCTCCCCACATGTGGAAAAAGTCCAGCACACTGACCGGGAGCCGCCTGGCTGCTGCCGAAGAGGA GAGCGGCATGAGCTCCAGTAAGCGCTTCCTGCGTTCTTGTTCGGCCTCCTGCATGCCCCATGGGGCAAGG GACACAGAGTGGCGGTCGGTCACGCTGCCTCGAGACCTGCCGTCTGCTGGCAAGCAGTTTGACTCATCCA CCTTTGGAGGGCACAAAAGCGAAAAGCCAGCTCTGCCTCGGAAACGCACCAGTGAGAGCAGGTCTGAGCA GGTGGCCAAAAGCACGGCGATGCCCCCTCCCCGGCTGGTGAAGAAGAACGAGGAGGCTGCTGAAGAAGGC TTCAAAGACACAGAATCCAGCCCTGGCTCCAGCCCTCCCAGCTTGACTCCCAAACTCCTCCGCAGGCAGG TCACTGCCTCTCCTTCCTCTGGCCTCTCTCACAAGGAAGAGGCCACCAAGGGCAGTGCCTCAGGCATGGG GACTCCGGCCACTGCAGAGCCAGCACCCCCCAGCAACAAAGTGGGCCTCAGCAAGGCCTCCTCTGAGGAG ATGCGCGTAAGGAGGCACAAGCACAGCTCGGAGTCCCCAGGGAGAGACAAGGGGCGACTGGCTAAGCTCA AGCCTGCCCCGCCGCCTCCTCCTGCCTGCACAGGAAAAGCAGGCAAGCCCGCACAGAGCCCCAGCCAAGA GGCCGGGGAGGCAGGGGGGCCCACAAAGACAAAATGCACGAGTCTGGCTATGGATGCTGTGAACACTGAC CCCACCAAGGCCGGCCCACCTGGAGAAGGACTGAGAAAGCCTGTGCCCCCATCTGTGCCAAAGCCCCAGT CGACGGCTAAGCCTCCAGGGACTCCCACCAGCCCGGTCTCCACCCCCTCCACAGCACCAGCTCCTTCACC CCTGGCTGGGGACCAGCAGCCATCTTCTGCCGCCTTCATCCCCCTCATATCAACCCGTGTGTCTCTTAGG AAGACCCGCCAGCCGCCAGAGCGCATTGCCAGTGGCACCATCACCAAGGGTGTGGTTCTGGACAGTACTG AGGCCCTGTGCCTTGCCATCTCCCGGAACTCAGAGCAGATGGCCAGCCACAGTGCTGTACTGGAGGCTGG CAAGAACCTGTACACTTTCTGTGTGAGCTATGTGGACTCTATCCAGCAGATGAGGAACAAGTTTGCCTTC CGTGAGGCTATCAACAAGCTGGAGAGCAACCTCCGAGAGCTGCAGATCTGCCCTGCCACAGCCTCCAGTG GGCCAGCTGCCACCCAAGACTTCAGCAAGCTGCTCAGCTCTGTGAAGGAGATCAGCGACATTGTCCGGAG G ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231317 representing NM_001283047 Red=Cloning site Green=Tags(s) MERTDITMKHKLGGGQYGEVYEGVWKKYSLTVAVKTLKEDTMEVEEFLKEAAVMKEIKHPNLVQLLGVCT REPPFYIITEFMTYGNLLDYLRECNRQEVSAVVLLYMATQISSAMEYLEKKNFIHRDLAARNCLVGENHL VKVADFGLSRLMTGDTYTAHAGAKFPIKWTAPESLAYNKFSIKSDVWAFGVLLWEIATYGMSPYPGIDLS QVYELLEKDYRMERPEGCPEKVYELMRACWQWNPSDRPSFAEIHQAFETMFQESSISDEVEKELGKRGTR GGAGSMLQAPELPTKTRTCRRAAEQKDAPDTPELLHTKGLGESDALDSEPAVSPLLPRKERGPPDGSLNE DERLLPRDRKTNLFSALIKKKKKMAPTPPKRSSSFREMDGQPDRRGASEDDSRELCNGPPALTSDAAEPT KSPKASNGAGVPNGAFREPGNSGFRSPHMWKKSSTLTGSRLAAAEEESGMSSSKRFLRSCSASCMPHGAR DTEWRSVTLPRDLPSAGKQFDSSTFGGHKSEKPALPRKRTSESRSEQVAKSTAMPPPRLVKKNEEAAEEG FKDTESSPGSSPPSLTPKLLRRQVTASPSSGLSHKEEATKGSASGMGTPATAEPAPPSNKVGLSKASSEE MRVRRHKHSSESPGRDKGRLAKLKPAPPPPPACTGKAGKPAQSPSQEAGEAGGPTKTKCTSLAMDAVNTD PTKAGPPGEGLRKPVPPSVPKPQSTAKPPGTPTSPVSTPSTAPAPSPLAGDQQPSSAAFIPLISTRVSLR KTRQPPERIASGTITKGVVLDSTEALCLAISRNSEQMASHSAVLEAGKNLYTFCVSYVDSIQQMRNKFAF REAINKLESNLRELQICPATASSGPAATQDFSKLLSSVKEISDIVRR TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001283047
ORF Size	2661 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001283047.1, NP_001269976.1
RefSeq Size	7306 bp
RefSeq ORF	2664 bp
Locus ID	11350
MW	96.9 kDa
Gene Summary	Non-receptor tyrosine-protein kinase that plays a role in many key processes linked to cell growth and survival such as cytoskeleton remodeling in response to extracellular stimuli, cell motility and adhesion, receptor endocytosis, autophagy, DNA damage response and apoptosis. Coordinates actin remodeling through tyrosine phosphorylation of proteins controlling cytoskeleton dynamics like WASF3 (involved in branch formation); ANXA1 (involved in membrane anchoring); DBN1, DBNL, CTTN, RAPH1 and ENAH (involved in signaling); or MAPT and PXN (microtubule-binding proteins). Phosphorylation of WASF3 is critical for the stimulation of lamellipodia formation and cell migration. Involved in the regulation of cell adhesion and motility through phosphorylation of key regulators of these processes such as BCAR1, CRK, CRKL, DOK1, EFS or NEDD9. Phosphorylates multiple receptor tyrosine kinases and more particularly promotes endocytosis of EGFR, facilitates the formation of neuromuscular synapses through MUSK, inhibits PDGFRB-mediated chemotaxis and modulates the endocytosis of activated B-cell receptor complexes. Other substrates which are involved in endocytosis regulation are the caveolin (CAV1) and RIN1. Moreover, ABL1 regulates the CBL family of ubiquitin ligases that drive receptor down-regulation and actin remodeling. Phosphorylation of CBL leads to increased EGFR stability. Involved in late-stage autophagy by regulating positively the trafficking and function of lysosomal components. ABL1 targets to mitochondria in response to oxidative stress and thereby mediates mitochondrial dysfunction and cell death. In response to oxidative stress, phosphorylates serine/threonine kinase PRKD2 at 'Tyr-717' (By similarity). ABL1 is also translocated in the nucleus where it has DNA-binding activity and is involved in DNA-damage response and apoptosis. Many substrates are known mediators of DNA repair: DDB1, DDB2, ERCC3, ERCC6, RAD9A, RAD51, RAD52 or WRN. Activates the proapoptotic pathway when the DNA damage is too severe to be repaired. Phosphorylates TP73, a primary regulator for this type of damage-induced apoptosis. Phosphorylates the caspase CASP9 on 'Tyr-191' and regulates its processing in the apoptotic response to DNA damage. Phosphorylates PSMA7 that leads to an inhibition of proteasomal activity and cell cycle transition blocks. Regulates T-cell differentiation in a TBX21-dependent manner (PubMed:21690296). Phosphorylates TBX21 on tyrosine residues leading to an enhancement of its transcriptional activator activity (PubMed:21690296).[UniProtKB/Swiss-Prot Function]

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