Ano1 (NM_001242349) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Tmem16a
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231486 ORF sequence Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGGACGCGCAGGACAGCGACATCGGCCTGGAGGGCTTGACCCCAGAGGGAACCTCTGCGGACCGAG AGTGCCAGCGGGGACCGGAAACAATCGCGCACGAGGCACAGGACGCAGGAACCCCCAACTCAGGTGACGC CACCGGGGTTGTGGATGGGGAGCGCGAGGCCACCATGAGGGTCCCCGAGAAGTACTCGACGCTCCCGGCG GAGGACCGCAGCGTCCACATCGTGAACATCTGCGCCATCGAGGACCTGGGCTACCTGCCGTCCGAGGGCA CGTTGCTGAACTCTCTGTCCGTGGACCCCGACGCCGAATGCAAGTATGGACTATACTTCAGGGATGGCAA ACGGAAGGTGGACTACATCTTGGTATACCATCACAAGAGAGCCTCAGGGAGCAGGACTCTGGCCAGGAGG GGACTACAAAATGACATGGTCCTGGGGACCCGCAGCGTCAGGCAGGACCAGCCCCTTCCCGGGAAGGGGA GCCCTGTGGATGCAGGCTCACCGGAAGTCCCCATGGATTACCATGAAGATGACAAACGCTTCAGACGGGA GGAATATGAGGGCAACCTGTTGGAGGCAGGCCTGGAGTTGGAGAATGACGAGGATACCAAAATCCATGGT GTCGGGTTTGTGAAGATCCATGCGCCCTGGCATGTGCTCTGTAGGGAAGCTGAGTTTTTGAAACTAAAGA TGCCCACAAAGAAGGTGTACCACATCAGTGAGACGCGAGGCCTCCTGAAAACCATCAACTCGGTTCTGCA GAAGATCACAGACCCCATCCAGCCCAAGGTGGCTGAGCACAGGCCACAGACCACAAAGAGGCTCTCCTAT CCCTTCTCCCGGGAGAAGCAACACCTATTCGACCTGACTGACAGGGACTCTTTTTTCGACAGCAAAACCC GGAGCACAATAGTCTATGAAATCCTGAAGAGAACAACGTGCACCAAGGCCAAGTACAGCATGGGTATCAC CAGCCTCCTGGCCAATGGCGTATACTCAGCTGCATACCCTCTGCACGATGGGGACTATGAGGGTGACAAC GTTGAGTTCAACGACAGGAAACTCCTGTATGAGGAATGGGCAAGTTACGGAGTCTTCTACAAATACCAGC CCATTGACCTGGTCAGGAAATACTTTGGTGAGAAGGTTGGCCTGTACTTTGCCTGGCTTGGAGCCTACAC CCAGATGCTCATCCCTGCCTCGATCGTGGGTGTCATTGTCTTTCTCTATGGATGTGCCACTGTGGACGAA AACATCCCCAGTATGGAGATGTGTGACCAGAGATACAACATCACCATGTGTCCTCTGTGTGACAAGACCT GCAGCTACTGGAAGATGAGCTCAGCCTGTGCCACAGCCCGTGCCAGTCACCTTTTTGATAACCCTGCCAC CGTCTTCTTCTCTGTGTTTATGGCCCTCTGGGCTGCCACTTTCATGGAGCACTGGAAACGGAAGCAGATG AGGCTCAACTACCGATGGGACCTCACAGGCTTCGAGGAGGAGGAGGATCATCCCAGAGCAGAGTATGAAG CCAGAGTCTTAGAGAAGTCACTGAGAAAAGAATCCAGAAACAAAGAGACCGACAAGGTGAAGCTGACCTG GAGGGACCGATTCCCAGCCTATTTCACCAATCTTGTCTCCATCATCTTCATGATCGCAGTGACATTTGCA ATCGTCCTCGGAGTTATCATCTATAGAATCTCCACAGCTGCAGCCTTGGCCATGAACTCCTCCCCGTCTG TGCGGTCCAACATCCGGGTTACAGTCACGGCCACCGCTGTTATCATCAACCTCGTGGTCATCATTCTGCT GGATGAAGTTTACGGCTGCATTGCCAGGTGGCTCACCAAGATTGAGGTCCCAAAGACAGAGAAGAGCTTT GAGGAGAGGCTAACCTTCAAGGCCTTCCTGCTCAAGTTTGTGAACTCTTACACTCCCATCTTCTATGTCG CCTTCTTCAAAGGCCGGTTTGTTGGTCGGCCCGGTGACTACGTGTACATCTTCCGCTCTTTCCGGATGGA GGAGTGTGCCCCGGGCGGCTGCCTCATGGAGCTCTGTATCCAGCTGAGCATCATTATGCTGGGCAAGCAG CTAATCCAGAACAATCTCTTCGAGATTGGCATCCCGAAGATGAAAAAGTTCATCCGCTACCTGAAGCTGC GCAGACAGAGCCCCTCAGACCGTGAAGAGTACGTGAAGCGGAAGCAGCGCTATGAGGTGGACTTCAACCT CGAACCTTTCGCCGGCCTCACGCCCGAGTACATGGAAATGATCATTCAGTTCGGCTTTGTCACCCTGTTT GTTGCGTCCTTCCCTCTGGCTCCACTCTTCGCCCTGCTAAACAACATCATTGAGATCCGCCTGGATGCCA AAAAGTTTGTCACCGAGCTACGGAGGCCAGTAGCCATCAGAGCCAAAGACATCGGCATCTGGTATAACAT CCTCAGAGGTGTTGGGAAGCTGGCTGTCATCATTAATGCCTTTGTGATCTCCTTCACGTCTGACTTCATC CCTCGCCTGGTGTACCTCTACATGTACAGTCAGAATGGGACCATGCACGGCTTCGTCAATCACACGCTCT CTTCCTTCAATGTCAGCGACTTCCAGAATGGCACAGCACCCAATGACCCACTGGACCTGGGCTATGAGGT TCAGATCTGCAGGTATAAAGATTACCGGGAACCCCCATGGTCAGAACACAAGTATGACATCTCCAAAGAC TTCTGGGCTGTCCTGGCCGCCCGACTGGCATTTGTCATTGTCTTCCAGAACCTGGTGATGTTCATGAGTG ACTTTGTGGACTGGGTGATCCCTGATATCCCCAAAGACATCAGCCAGCAGATCCACAAAGAGAAGGTTCT CATGGTGGAGCTGTTTATGCGTGAGGAGCAGGGCAAGCAGCAGCTACTGGACACATGGATGGAGAAGGAG AAGCCAAGGGATGTGCCTTGTAACAACCACAGCCCCACAACCCACCCAGAGGCAGGCGACGGCAGCCCAG TCCCCAGCTACGAGTACCATGGGGACGCGCTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231486 protein sequence Red=Cloning site Green=Tags(s) MQDAQDSDIGLEGLTPEGTSADRECQRGPETIAHEAQDAGTPNSGDATGVVDGEREATMRVPEKYSTLPA EDRSVHIVNICAIEDLGYLPSEGTLLNSLSVDPDAECKYGLYFRDGKRKVDYILVYHHKRASGSRTLARR GLQNDMVLGTRSVRQDQPLPGKGSPVDAGSPEVPMDYHEDDKRFRREEYEGNLLEAGLELENDEDTKIHG VGFVKIHAPWHVLCREAEFLKLKMPTKKVYHISETRGLLKTINSVLQKITDPIQPKVAEHRPQTTKRLSY PFSREKQHLFDLTDRDSFFDSKTRSTIVYEILKRTTCTKAKYSMGITSLLANGVYSAAYPLHDGDYEGDN VEFNDRKLLYEEWASYGVFYKYQPIDLVRKYFGEKVGLYFAWLGAYTQMLIPASIVGVIVFLYGCATVDE NIPSMEMCDQRYNITMCPLCDKTCSYWKMSSACATARASHLFDNPATVFFSVFMALWAATFMEHWKRKQM RLNYRWDLTGFEEEEDHPRAEYEARVLEKSLRKESRNKETDKVKLTWRDRFPAYFTNLVSIIFMIAVTFA IVLGVIIYRISTAAALAMNSSPSVRSNIRVTVTATAVIINLVVIILLDEVYGCIARWLTKIEVPKTEKSF EERLTFKAFLLKFVNSYTPIFYVAFFKGRFVGRPGDYVYIFRSFRMEECAPGGCLMELCIQLSIIMLGKQ LIQNNLFEIGIPKMKKFIRYLKLRRQSPSDREEYVKRKQRYEVDFNLEPFAGLTPEYMEMIIQFGFVTLF VASFPLAPLFALLNNIIEIRLDAKKFVTELRRPVAIRAKDIGIWYNILRGVGKLAVIINAFVISFTSDFI PRLVYLYMYSQNGTMHGFVNHTLSSFNVSDFQNGTAPNDPLDLGYEVQICRYKDYREPPWSEHKYDISKD FWAVLAARLAFVIVFQNLVMFMSDFVDWVIPDIPKDISQQIHKEKVLMVELFMREEQGKQQLLDTWMEKE KPRDVPCNNHSPTTHPEAGDGSPVPSYEYHGDAL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001242349
ORF Size	3042 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001242349.1, NP_001229278.1
RefSeq Size	4605 bp
RefSeq ORF	3045 bp
Locus ID	101772
UniProt ID	Q8BHY3
MW	116.4 kDa
Gene Summary	Calcium-activated chloride channel (CaCC) which plays an important role in transepithelial anion transport and smooth muscle contraction. Required for the normal functioning of the interstitial cells of Cajal (ICCs) which generate electrical pacemaker activity in gastrointestinal smooth muscles. Acts as a major contributor to basal and stimulated chloride conductance in airway epithelial cells and plays an important role in tracheal cartilage development.[UniProtKB/Swiss-Prot Function]

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