Smarca1 (NM_001290708) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	5730494M04Rik; Snf2l
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231538 representing NM_001290708 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCCGGACACGGCCACAGAGGCAGCCACCGTGGCAGTTTCGGATGCCAGAGCCACAGTCGTGGTTG TAGAGGATGAGCAGCCCGGGCCGTCCACCTTTAAAGAGGAGGGAGCGGCTGCTGCCGCCACCGAAGGCAC CACGGCCACTGAGAAGGGCGAGAAGAAGGAGAAAATCACTTCTCCATTTCAACTCAAACTTGCTGCTAAA GCTTCCAAATCTGAAAAGGAAATGGATCCAGAATATGAAGAGAAAATGGTAAATATGCCCTTGAAAGCAG ACCGCGCAAAGAGATTTGAGTTTTTACTGAAGCAGACGGAACTTTTTGCACATTTCATTCAGCCTTCAGC ACAGAAGTCTCCAACATCTCCTCTCAACATGAAACTGGCTCGTCCTCGAGTAAAGAAAGATGACAAGCAG AGCTTGATTTCCGTTGGAGACTATCGCCACAGGCGTACAGAACAAGAAGAGGATGAGGAGCTTCTCTCAG AGAGTAGGAAAACATCGAATGTGTGTGTCAGATTCGAGGTCTCCCCTTCCTATGTGAAAGGAGGACCGCT GAGAGATTATCAGATCCGAGGACTAAACTGGTTGATTTCTTTGTATGAAAATGGAGTCAATGGTATTTTA GCTGATGAAATGGGTCTTGGGAAGACTTTACAAACCATTGCCTTGCTTGGTTACCTGAAGCACTACAGAA ACATTCCTGGACCCCACATGGTTTTAGTTCCCAAGTCTACTTTACATAACTGGATGAATGAATTTAAACG ATGGGTTCCATCTCTCCGTGTTATTTGTTTCGTTGGAGACAAAGATGTGAGAGCAGCTTTTATTCGTGAT GAAATGATGCCAGGAGAGTGGGATGTTTGCGTGACTTCTTATGAGATGGTAATTAAAGAAAAATCTGTGT TTAAAAAGTTTCACTGGCGATACTTAGTCATCGATGAAGCTCACAGAATAAAAAATGAAAAATCTAAGCT TTCAGAGATTGTTCGTGAGTTCAAGTCAACTAATCGCTTACTTCTAACTGGGACACCTTTGCAGAATAAC TTGCATGAGCTCTGGGCATTACTTAATTTTTTATTGCCGGATGTCTTTAATTCTGCAGATGACTTTGATT CTTGGTTTGACACTAAAAATTGCCTTGGTGATCAAAAGCTTGTGGAAAGACTCCATGCAGTTTTAAAACC ATTTTTGTTACGCCGGATAAAAACCGATGTAGAGAAGAGTCTGCCTCCTAAGAAGGAAATAAAGATTTAC TTGGGCCTGAGTAAAATGCAACGAGAATGGTATACAAAAATTCTGATGAAAGATATTGATGTCTTAAACT CTTCTGGCAAGATGGACAAGATGCGACTCTTGAATATTCTGATGCAGCTCCGAAAGTGTTGTAATCATCC TTATCTGTTTGATGGAGCAGAGCCTGGTCCACCTTATACGACAGATGAGCACATTGTCGGCAATAGTGGT AAAATGGTCGCTCTGGATAAGCTATTGGCGAGAATTAAAGAACAGGGATCAAGAGTTCTCATCTTTAGCC AGATGACTCGACTGTTAGACATTTTGGAGGATTATTGTATGTGGCGTGGTTATGAGTATTCTCGACTGGA TGGACAAACCCCACATGAAGAAAGAGAGGAAGCGATAGATGCCTTCAATGCTCCAAATAGCAGCAAATTC ATCTTTATGCTGAGTACTAGGGCTGGAGGACTCGGAATTAACTTGGCAAGTGCTGATGTGGTCATCCTAT ATGATTCAGACTGGAATCCACAGGTTGATTTACAAGCTATGGATCGGGCACATCGCATTGGTCAGAAGAA GCCAGTACGAGTCTTCCGTCTCATTACAGACAACACAGTGGAAGAGAGGATTGTAGAAAGAGCTGAGATA AAACTGAGACTTGATTCAATTGTTATACAGCAAGGAAGACTCATTGACCAACAATCTAACAAGCTGGCAA AAGAGGAAATGCTACAAATGATCCGTCATGGAGCTACACATGTTTTTGCCTGTAAAGAAAGTGAGCTGAC AGATGAGGACATTGTAACTATTCTGGAAAGAGGAGAAAAGAAGACTGCAGAGATGAATGAACGCATGCAG AAAATGGGAGAGTCATCTCTAAGAAATTTTAGAATGGACCTTGAACAAAGTTTGTACAAGTTTGAAGGAG AAGATTACAGAGAAAAACAAAAGCTGGGTACAGTGGAATGGATTGAACCTCCTAAACGAGAACGCAAAGC CAACTATGCAGTGGATGCCTACTTTAGAGAGGCTTTACGTGTCAGCGAGCCAAAGATCCCAAAGGCTCCT CGGCCTCCAAAACAGCCAAATGTTCAAGATTTTCAATTTTTTCCACCGCGCTTATTTGAGCTCCTGGAAA AGGAAATTCTTTATTATCGGAAGACTATAGGTTACAAGGTCCCAAGAAATCCTGAGATCCCCAACCCAGC TATAGCTCAAAGAGAAGAGCAAAAAAAGATTGATGGAGCGGAACCTCTTACACCACAAGAGACTGAAGAG AAGGATAAACTTCTCACACAAGGTTTCACAAACTGGACTAAGCGAGATTTTAACCAGTTTATTAAAGCTA ATGAGAAGTATGGGAGAGATGACATTGATAATATAGCTCGAGAGGTGGAGGGCAAGTCCCCTGAGGAGGT CATGGAGTATTCAGCTGTATTTTGGGAACGCTGCAATGAATTACAGGACATTGAGAAAATTATGGCTCAA ATTGAACGTGGAGAAGCAAGAATTCAGCGAAGGATCAGTATTAAGAAAGCACTGGATGCCAAAATTGCAA GATACAAGGCTCCATTTCATCAGTTGCGTATTCAGTATGGAACCAGTAAAGGAAAGAACTATACTGAAGA GGAAGACAGATTCTTGATCTGTATGTTACACAAAATGGGTTTTGACAGAGAAAATGTATATGAAGAATTA AGGCAGTGTGTACGGAATGCTCCCCAGTTTAGATTTGACTGGTTCATCAAGTCGAGAACTGCCATGGAAT TTCAGAGACGCTGTAATACTTTGATTTCATTGATTGAAAAAGAAAATATGGAAATTGAGGAAAGAGAGAG AGCAGAAAAGAAGAAACGGGCAACTAAAACTCCAATGTCACAGAAAAGAAAAGCAGAGTCAGCTACTGAG AGCTCTGGAAGGAAGGATGTCAAGAAGGTGAAATCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231538 representing NM_001290708 Red=Cloning site Green=Tags(s) MEPDTATEAATVAVSDARATVVVVEDEQPGPSTFKEEGAAAAATEGTTATEKGEKKEKITSPFQLKLAAK ASKSEKEMDPEYEEKMVNMPLKADRAKRFEFLLKQTELFAHFIQPSAQKSPTSPLNMKLARPRVKKDDKQ SLISVGDYRHRRTEQEEDEELLSESRKTSNVCVRFEVSPSYVKGGPLRDYQIRGLNWLISLYENGVNGIL ADEMGLGKTLQTIALLGYLKHYRNIPGPHMVLVPKSTLHNWMNEFKRWVPSLRVICFVGDKDVRAAFIRD EMMPGEWDVCVTSYEMVIKEKSVFKKFHWRYLVIDEAHRIKNEKSKLSEIVREFKSTNRLLLTGTPLQNN LHELWALLNFLLPDVFNSADDFDSWFDTKNCLGDQKLVERLHAVLKPFLLRRIKTDVEKSLPPKKEIKIY LGLSKMQREWYTKILMKDIDVLNSSGKMDKMRLLNILMQLRKCCNHPYLFDGAEPGPPYTTDEHIVGNSG KMVALDKLLARIKEQGSRVLIFSQMTRLLDILEDYCMWRGYEYSRLDGQTPHEEREEAIDAFNAPNSSKF IFMLSTRAGGLGINLASADVVILYDSDWNPQVDLQAMDRAHRIGQKKPVRVFRLITDNTVEERIVERAEI KLRLDSIVIQQGRLIDQQSNKLAKEEMLQMIRHGATHVFACKESELTDEDIVTILERGEKKTAEMNERMQ KMGESSLRNFRMDLEQSLYKFEGEDYREKQKLGTVEWIEPPKRERKANYAVDAYFREALRVSEPKIPKAP RPPKQPNVQDFQFFPPRLFELLEKEILYYRKTIGYKVPRNPEIPNPAIAQREEQKKIDGAEPLTPQETEE KDKLLTQGFTNWTKRDFNQFIKANEKYGRDDIDNIAREVEGKSPEEVMEYSAVFWERCNELQDIEKIMAQ IERGEARIQRRISIKKALDAKIARYKAPFHQLRIQYGTSKGKNYTEEEDRFLICMLHKMGFDRENVYEEL RQCVRNAPQFRFDWFIKSRTAMEFQRRCNTLISLIEKENMEIEERERAEKKKRATKTPMSQKRKAESATE SSGRKDVKKVKS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001290708
ORF Size	3186 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001290708.2
RefSeq Size	4024 bp
RefSeq ORF	3189 bp
Locus ID	93761
UniProt ID	Q6PGB8
MW	123.9 kDa
Gene Summary	Energy-transducing component of the NURF (nucleosome-remodeling factor) and CERF (CECR2-containing-remodeling factor) complexes, which facilitate the perturbation of chromatin structure in an ATP-dependent manner.[UniProtKB/Swiss-Prot Function]

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