Map4 (NM_001205332) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	AA407148; MAP-4; Mtap-4; Mtap4
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231562 representing NM_001205332 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGACCTCAGTCTTGTGGATGCGTTGACAGAACCACCTCCAGAAATTGAGGGAGAAATAAAGCGAG ACTTCATGGCTGCGCTGGAGGCAGAGCCCTATGATGACATCGTGGGAGAAACTGTGGAGAAAACTGAGTT TATTCCTCTCCTGGATGGTGATGAGAAAACCGGGAACTCAGAGTCCAAAAAGAAACCCTGCTTAGACACT AGCCAGGTTGAAGGTATCCCATCTTCTAAACCAACACTCCTAGCCAATGGTGATCATGGAATGGAGGGGA ATAACACTGCAGGGTCTCCAACTGACTTCCTTGAAGAGAGAGTGGACTATCCGGATTATCAGAGCAGCCA GAACTGGCCAGAAGATGCAAGCTTTTGTTTCCAGCCTCAGCAAGTGTTAGATACTGACCAGGCTGAGCCC TTTAACGAGCACCGTGATGATGGTTTGGCAGATCTGCTCTTTGTCTCCAGTGGACCCACGAACGCTTCTG CATTTACAGAGCGAGACAATCCTTCAGAAGACAGTTACGGTATGCTTCCCTGTGACTCATTTGCTTCCAC GGCTGTTGTATCTCAGGAGTGGTCTGTGGGAGCCCCAAACTCTCCATGTTCAGAGTCCTGTGTCTCCCCA GAGGTTACTATAGAAACCCTACAGCCAGCAACAGAGCTCTCCAAGGCAGCAGAAGTGGAATCAGTGAAAG AGCAGCTGCCAGCTAAAGCATTGGAAACGATGGCAGAGCAGACCACTGATGTGGTGCACTCTCCATCCAC AGACACAACACCAGGCCCAGACACAGAGGCAGCACTGGCTAAAGACATAGAAGAGATCACCAAGCCAGAT GTGATATTGGCAAATGTCACGCAGCCATCTACTGAATCGGATATGTTCCTGGCCCAGGACATGGAACTAC TCACAGGAACAGAGGCAGCCCACGCTAACAATATCATATTGCCTACAGAACCAGACGAATCTTCAACCAA GGATGTAGCACCACCTATGGAAGAAGAAATTGTCCCAGGCAATGATACGACATCCCCCAAAGAAACAGAG ACAACACTTCCAATAAAAATGGACTTGGCACCACCTGAGGATGTGTTACTTACCAAAGAAACAGAACTAG CCCCAGCCAAGGGCATGGTTTCACTCTCAGAAATAGAAGAGGCTCTGGCAAAGAATGATGAGTCCTCTGC AGAAATACCTGTGGCTCAGGAGACAGTGGTCTCAGAAACAGAGGTGGTCCTGGCAACAGAAGTGGTACTG CCCTCAGATCCCATAACAACATTGACAAAGGATGTGACACTCCCCTTAGAAGCAGAGAGACCGTTGGTGA CGGACATGACTCCATCTCTGGAAACAGAAATGACCCTAGGCAAAGAGACAGCTCCACCCACAGAAACAAA TTTGGGCATGGCCAAAGACATGTCTCCACTCCCAGAATCAGAAGTGACTCTGGGCAAGGACGTGGTTATA CTTCCAGAAACAAAGGTGGCTGAGTTTAACAATGTGACTCCACTTTCAGAAGAAGAGGTAACCTCAGTCA AGGACATGTCTCCGTCTGCAGAAACAGAGGCTCCCCTGGCTAAGAATGCTGATCTGCACTCAGGAACAGA GCTGATTGTGGACAACAGCATGGCTCCAGCCTCCGATCTTGCACTGCCCTTGGAAACAAAAGTAGCAACA GTTCCAATTAAAGACAAAGGAACTGTACAGACTGAAGAAAAACCACGTGAAGACTCCCAGTTAGCATCTA TGCAGCACAAGGGACAGTCAACAGTACCTCCTTGCACGGCTTCACCAGAACCAGTCAAAGCTGCAGAACA AATGTCTACCTTACCAATAGATGCACCTTCTCCATTAGAGAACTTAGAGCAGAAGGAAACGCCTGGCAGC CAGCCTTCTGAGCCTTGCTCAGGAGTATCCCGGCAAGAAGAAGCAAAGGCTGCTGTAGGTGTGACTGGAA ATGACATCACTACCCCGCCAAACAAGGAGCCACCACCAAGCCCAGAAAAGAAAGCAAAGCCTTTGGCCAC CACTCAACCTGCAAAGACTTCAACATCGAAAGCCAAAACACAGCCCACTTCTCTCCCTAAGCAACCAGCT CCCACCACCTCTGGTGGGTTGAATAAAAAACCCATGAGCCTCGCCTCAGGCTCAGTGCCAGCTGCCCCAC ACAAACGCCCTGCTGCTGCCACTGCTACTGCCAGGCCTTCCACCCTACCTGCCAGAGACGTGAAGCCAAA GCCAATTACAGAAGCTAAGGTTGCCGAAAAGCGGACCTCTCCATCCAAGCCTTCATCTGCCCCAGCCCTC AAACCTGGACCTAAAACCACCCCAACCGTTTCAAAAGCCACATCTCCCTCAACTCTTGTTTCCACTGGAC CAAGTAGTAGAAGTCCAGCTACAACTCTGCCTAAGAGGCCAACCAGCATCAAGACTGAGGGGAAACCTGC TGATGTCAAAAGGATGACTGCTAAGTCTGCCTCAGCTGACTTGAGTCGCTCAAAGACCACCTCTGCCAGT TCTGTGAAGAGAAACACCACTCCCACTGGGGCAGCACCCCCAGCAGGGATGACTTCCACTCGAGTCAAGC CCATGTCTGCACCTAGCCGCTCTTCTGGGGCTCTTTCTGTGGACAAGAAGCCCACTTCCACTAAGCCTAG CTCCTCTGCTCCCAGGGTGAGCCGCCTGGCCACAACTGTTTCTGCCCCTGACCTGAAGAGTGTTCGCTCC AAGGTCGGCTCTACAGAAAACATCAAACACCAGCCTGGAGGAGGCCGGGTCCAGATAGTATCCAAAAAAG TGAGCTACAGCCATATTCAATCCAAGTGTGGTTCCAAGGACAATATTAAGCATGTCCCTGGAGGTGGCAA TGTTCAGATTCAGAACAAGAAAGTGGACATATCCAAGGTCTCCTCCAAGTGTGGGTCCAAAGCTAATATC AAGCACAAGCCTGGTGGAGGAGATGTCAAGATTGAAAGTCAGAAGTTGAACTTCAAGGAGAAGGCCCAAG CCAAAGTGGGATCCCTTGATAACGTGGGCCACTTGCCTGCAGGAGGTGCCGTGAAGACTGAGGGCGGTGG CAGTGAGGCCCTTCCGTGTCCAGGCCCCCCCGCTGGGGAGGAGCCAGTCATCCCTGAGGCTGCGCCTGAC GCTGGCGCCCCTACTTCAGCCAGTGGCCTCAGTGGCCACACCACCCTGTCAGGGGGTGGTGACCAAAGGG AGCCCCAGACCTTGGACAGCCAGATCCAGGAGACAAAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231562 representing NM_001205332 Red=Cloning site Green=Tags(s) MADLSLVDALTEPPPEIEGEIKRDFMAALEAEPYDDIVGETVEKTEFIPLLDGDEKTGNSESKKKPCLDT SQVEGIPSSKPTLLANGDHGMEGNNTAGSPTDFLEERVDYPDYQSSQNWPEDASFCFQPQQVLDTDQAEP FNEHRDDGLADLLFVSSGPTNASAFTERDNPSEDSYGMLPCDSFASTAVVSQEWSVGAPNSPCSESCVSP EVTIETLQPATELSKAAEVESVKEQLPAKALETMAEQTTDVVHSPSTDTTPGPDTEAALAKDIEEITKPD VILANVTQPSTESDMFLAQDMELLTGTEAAHANNIILPTEPDESSTKDVAPPMEEEIVPGNDTTSPKETE TTLPIKMDLAPPEDVLLTKETELAPAKGMVSLSEIEEALAKNDESSAEIPVAQETVVSETEVVLATEVVL PSDPITTLTKDVTLPLEAERPLVTDMTPSLETEMTLGKETAPPTETNLGMAKDMSPLPESEVTLGKDVVI LPETKVAEFNNVTPLSEEEVTSVKDMSPSAETEAPLAKNADLHSGTELIVDNSMAPASDLALPLETKVAT VPIKDKGTVQTEEKPREDSQLASMQHKGQSTVPPCTASPEPVKAAEQMSTLPIDAPSPLENLEQKETPGS QPSEPCSGVSRQEEAKAAVGVTGNDITTPPNKEPPPSPEKKAKPLATTQPAKTSTSKAKTQPTSLPKQPA PTTSGGLNKKPMSLASGSVPAAPHKRPAAATATARPSTLPARDVKPKPITEAKVAEKRTSPSKPSSAPAL KPGPKTTPTVSKATSPSTLVSTGPSSRSPATTLPKRPTSIKTEGKPADVKRMTAKSASADLSRSKTTSAS SVKRNTTPTGAAPPAGMTSTRVKPMSAPSRSSGALSVDKKPTSTKPSSSAPRVSRLATTVSAPDLKSVRS KVGSTENIKHQPGGGRVQIVSKKVSYSHIQSKCGSKDNIKHVPGGGNVQIQNKKVDISKVSSKCGSKANI KHKPGGGDVKIESQKLNFKEKAQAKVGSLDNVGHLPAGGAVKTEGGGSEALPCPGPPAGEEPVIPEAAPD AGAPTSASGLSGHTTLSGGGDQREPQTLDSQIQETN TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001205332
ORF Size	3258 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001205332.1, NP_001192261.1
RefSeq Size	5556 bp
RefSeq ORF	3261 bp
Locus ID	17758
UniProt ID	P27546
MW	114.1 kDa
Gene Summary	Non-neuronal microtubule-associated protein. Promotes microtubule assembly.[UniProtKB/Swiss-Prot Function]

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