Usp8 (NM_001252580) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	AI574262; AW557536; mKIAA0055; Ubpy
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231571 representing NM_001252580 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCTGCTGTAGCTTCAGTTCCTAAAGAACTCTACCTCAGTTCTTCACTAAAAGACCTCAATAAGAAGA CCGAAGTTAAACCTGAGAAAACCAGCACCAAGAATACCAAGTTTTGTAAATTCTCTTCGTTTCTAAGTTA TATACACAGCGCGCAGAAGATCTTCAAGACAGCAGAAGAATGCAGACTAGATCGTGATGAGGAAAGGGCC TATGTGCTTTATATGAAATATGTGGCAGTTTATAATCTTATCAAAAAGAGACCTGATTTCAAGCAACAGC AGGATTATTATCTTTCAATACTTGGACCTGCAAACATCAAAAAAGCTATTGAAGAAGCTGAAAGACTCTC CGAAAGCCTTAAACTAAGATACGAAGAGGCTGAAGTTCGGAAACAACTTGAAGAAAAGGACAGACGGGAG GAAGAGCAGCTGCAGCAACAGAAAAGGCAGGAGATGGGGAGAGAGGACAGCGGTGCGGCAGCCAAACGCT CTGTGGAAAATTTACTGGATTCCAAAACCAAAACCCAAAGGATTAACGGCGAGAAGAGTGAAGGAGCTGC AGCTGCAGAGAGAGGAGCCATCACAGCAAAGGAACTATATACGATGATGATGGATAAAAACACAAGCTTA ATTATAATGGATGCTCGAAAAATACAGGATTATCAGCATTCCTGTATCTTGGATTCTCTCAGTGTTCCTG AAGAAGCTATCAGTCCAGGAGTCACTGCTAGTTGGATTGAAGCAAACCTCTCAGATGATTCTAAAGACAC ATGGAAAAAGAGGGGGAGTGTGGACTATGTGGTCCTTCTCGACTGGTTTAGTTCAGCGAAAGATTTGCTG CTTGGGACCACTCTACGGAGTCTGAAAGATGCACTTTTCAAGTGGGAAAGTAAAACTGTCCTGCGCCATG AGCCTTTGGTGTTGGAGGGCGGCTATGAAAACTGGCTGCTTTGCTACCCGCAGTTTACAACCAATGCTAA GGTCACTCCACCCCCTCGGAGCAGAGCTGAAGAGGTGTCTGTCTCATTGGATTTTACTTATCCCTCATTG GAAGAACCAGTTCCTTCCAAACTTCCTACCCAGATGCCACCTCCTCCTATAGAAACAAATGAAAAGGCAC TGTTGGTAACTGATCAAGATGAAAAGCTGAGACTGTCAACCCAGCCAGCTCTAGCTGGACCTGGTGCGGC TCCCAGAGCTGAAGCCTCACCCATAATTCAGCCAGCGCCTGCTACAAAGAGTGTTCCACAGGTTGATCGT ACGAAAAAACCGTCAGTCAAGTTGCCTGAAGATCATAGAATAAAATCTGAAAATACAGATCAGAGTGGAA GAGTTCTTTCTGATCGATCCACCAAGCCAGTATTTCCCTCTCCAACCACCATGTTAACAGATGAAGAAAA GGCTCGTATTCATCAAGAAACTGCCCTTCTTATGGAAAAGAATAAACAGGAGAAGGAACTTTGGGACAAG CAGCAGAAGGAACAGAAAGAGAAGCTGAGAAGGGAGGAACAAGAGCGCAAAGCTGGAAAGACACAGGATG CAGATGAACGTGACTCCACTGAGAATCAGCACAAAGCAAAGGATGGACAAGAGAAAAAAGACAGCAAACA GACCAAGACGGAAGACAGAGAGCTTTCAGCAGACGGGGCCCAGGAAGCCACAGGAACACAAAGACAAAGT AAGAGTGAGCATGAAGCTTCTGATGCTAAGGTACCTGTGGAAGGTAAAAGGTGTCCCACGTCAGAGGCGC AGAAGAGGCCAGCAGATGTGTCCCCTGCATCCGTGTCAGGAGAGCTGAATGCAGGCAAGGCTCAGCGAGA ACCTTTGACGAGAGCACGAAGTGAAGAAATGGGGAGAATTGTGCCGGGACTGCCTTTGGGCTGGGCCAAG TTTCTTGATCCAATCACCGGGACCTTTCGTTACTACCATTCCCCCACAAACACAGTTCATATGTATCCAC CTGAAATGGCTCCTTCGTCTGCACCTCCTTCCACCCCGCCCACTCACAAAGTCAAGCCCCAGGTCCCTGC CGAGCGGGACAGGGAGCCATCGAAACTGAAGCGCTCCTACTCCTCACCAGATATCACTCAGGCCCTGCAG GAGGAGGAGAAGAGGAGGCCGGCAGTGACCCCGATGGTCAACCGGGAAAACAAGCCACCATGTTACCCTA AAGCTGAGATTTCGAGGCTTTCTGCTTCTCAGATTCGGAACCTCAATCCTGTATTTGGAGGATCAGGACC AGCTCTTACTGGACTTCGTAATTTGGGAAATACTTGTTACATGAACTCAATCTTGCAGTGCCTGTGTAAT GCTCCACATCTGGCTGATTATTTCAACCGAAACTGCTACCAGGATGATATCAACAGGTCAAATTTGTTGG GGCATAAAGGTGAAGTGGCAGAAGAATTTGGTATAATCATGAAAGCACTGTGGACAGGACAGTATAGATA CATCAGTCCAAAGGACTTTAAAGTCACCATTGGTAAGATTAATGACCAGTTTGCAGGCTCCAGCCAACAG GATTCACAAGAGCTGCTTCTGTTCCTCATGGATGGCCTGCATGAGGATCTGAATAAGGCTGACAATCGGA AGAGGCACAAGGAAGAGAACAACGAGCACCTGGATGACCTGCAGGCGGCCGAGCACGCCTGGCAGAAGCA CAAGCAGCTCAACGAGTCCATCATCGTGGCCCTGTTCCAGGGCCAGTTCAAGTCCACAGTGCAGTGCCTC ACCTGCCGCAGGAGGTCGCGCACCTTCGAGGCCTTCATGTACTTGTCTTTGCCGCTAGCATCCACAAGTA AATGTACTCTACAGGACTGCCTTAGATTATTTTCCAAAGAAGAAAAGCTTACAGATAACAACAGATTTTA CTGCAGCCATTGCCGAGCTCGGCGGGATTCTCTAAAGAAAATAGAAATCTGGAAATTACCTCCTGTGCTG TTAGTGCACCTGAAACGATTTTCCTATGACGGCAGGTGGAAGCAGAAGCTGCAAACATCCGTGGATTTCC CATTGGAAAATCTTGACCTGTCACAGTACGTTATTGGTCCAAAAAACAGCTTGAAGAAATATAACTTGTT TTCTGTTTCAAACCACTACGGCGGGCTAGACGGAGGCCACTACACAGCCTACTGTAAGAACGCGGCAAGG CAGCGCTGGTTTAAGTTTGATGACCATGAAGTTTCTGATATCTCTGTGTCTTCTGTGAGGTCATCAGCAG CTTATATCCTCTTTTATACTTCCCTGGGACCACGCATAACTGATGTAGCCACA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231571 representing NM_001252580 Red=Cloning site Green=Tags(s) MPAVASVPKELYLSSSLKDLNKKTEVKPEKTSTKNTKFCKFSSFLSYIHSAQKIFKTAEECRLDRDEERA YVLYMKYVAVYNLIKKRPDFKQQQDYYLSILGPANIKKAIEEAERLSESLKLRYEEAEVRKQLEEKDRRE EEQLQQQKRQEMGREDSGAAAKRSVENLLDSKTKTQRINGEKSEGAAAAERGAITAKELYTMMMDKNTSL IIMDARKIQDYQHSCILDSLSVPEEAISPGVTASWIEANLSDDSKDTWKKRGSVDYVVLLDWFSSAKDLL LGTTLRSLKDALFKWESKTVLRHEPLVLEGGYENWLLCYPQFTTNAKVTPPPRSRAEEVSVSLDFTYPSL EEPVPSKLPTQMPPPPIETNEKALLVTDQDEKLRLSTQPALAGPGAAPRAEASPIIQPAPATKSVPQVDR TKKPSVKLPEDHRIKSENTDQSGRVLSDRSTKPVFPSPTTMLTDEEKARIHQETALLMEKNKQEKELWDK QQKEQKEKLRREEQERKAGKTQDADERDSTENQHKAKDGQEKKDSKQTKTEDRELSADGAQEATGTQRQS KSEHEASDAKVPVEGKRCPTSEAQKRPADVSPASVSGELNAGKAQREPLTRARSEEMGRIVPGLPLGWAK FLDPITGTFRYYHSPTNTVHMYPPEMAPSSAPPSTPPTHKVKPQVPAERDREPSKLKRSYSSPDITQALQ EEEKRRPAVTPMVNRENKPPCYPKAEISRLSASQIRNLNPVFGGSGPALTGLRNLGNTCYMNSILQCLCN APHLADYFNRNCYQDDINRSNLLGHKGEVAEEFGIIMKALWTGQYRYISPKDFKVTIGKINDQFAGSSQQ DSQELLLFLMDGLHEDLNKADNRKRHKEENNEHLDDLQAAEHAWQKHKQLNESIIVALFQGQFKSTVQCL TCRRRSRTFEAFMYLSLPLASTSKCTLQDCLRLFSKEEKLTDNNRFYCSHCRARRDSLKKIEIWKLPPVL LVHLKRFSYDGRWKQKLQTSVDFPLENLDLSQYVIGPKNSLKKYNLFSVSNHYGGLDGGHYTAYCKNAAR QRWFKFDDHEVSDISVSSVRSSAAYILFYTSLGPRITDVAT TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001252580
ORF Size	3273 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001252580.1, NP_001239509.1
RefSeq Size	4210 bp
RefSeq ORF	3276 bp
Locus ID	84092
MW	124.3 kDa
Gene Summary	Hydrolase that can remove conjugated ubiquitin from proteins and therefore plays an important regulatory role at the level of protein turnover by preventing degradation. Converts both 'Lys-48' an 'Lys-63'-linked ubiquitin chains. Catalytic activity is enhanced in the M phase. Involved in cell proliferation. Required to enter into S phase in response to serum stimulation. May regulate T-cell anergy mediated by RNF128 via the formation of a complex containing RNF128 and OTUB1. Probably regulates the stability of STAM2 and RASGRF1. Regulates endosomal ubiquitin dynamics, cargo sorting, membrane traffic at early endosomes, and maintenance of ESCRT-0 stability. The level of protein ubiquitination on endosomes is essential for maintaining the morphology of the organelle. Deubiquitinates EPS15 and controles tyrosine kinase stability. Removes conjugated ubiquitin from EGFR thus regulating EGFR degradation and downstream MAPK signaling. Involved in acrosome biogenesis through interaction with the spermatid ESCRT-0 complex and microtubules. Deubiquitinates BIRC6/bruce and KIF23/MKLP1 (By similarity). Deubiquitinates BACE1 which inhibits BACE1 lysosomal degradation and modulates BACE-mediated APP cleavage and amyloid-beta formation (By similarity).[UniProtKB/Swiss-Prot Function]

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